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1.
近年来,空气污染已成为长三角地区最关键的环境问题之一,气溶胶颗粒物(PM)是最主要的污染物之一。生物气溶胶作为颗粒物的重要组成部分,可能对空气质量和人体健康产生不利影响。利用高通量测序方法研究了江苏省泰州2019年11月至2020年1月期间发生沙尘和霾污染时生物气溶胶中细菌群落结构组成特征。结果表明:冬季沙尘天和霾天气溶胶细菌群落结构及多样性呈现不同的变化特征,霾天细菌群落多样性、均匀度以及基于进化的多样性明显高于夏季优良天,粒径差异增大;沙尘天细菌群落结构变化和霾天正好相反,不仅大气微生物丰富度会降低,细菌群落结构的多样性和均匀度也会降低。优良天粗细颗粒的细菌群落组成和优势种相似度较高,沙尘和霾污染天粗细颗粒间的细菌组成差异较大,病原细菌的种类和相对丰度也明显增加,优势类群中致病菌浓度上升的主要有假单胞菌属(Pseudomonas)、雷氏菌属(Ralstonia)、肠球菌属(Enterococcus)、拟杆菌属(Bacteroides)、埃希氏菌-志贺氏菌属(Escherichia-Shigella)、链球菌属(Streptococcus)等,增加了人类、动物及农业生产活动潜在的暴露风险。基于丰度热图分析污染天各粒径的细菌群落结构相似性,沙尘天大部分粒径聚在一起,粒径间的细菌组成相似性较高;霾天样品的粒径分布总体较分散,随着污染加重,粒径差异有增大的趋势。  相似文献   

2.
结合《生活饮用水卫生标准》(GB 5749-2006),与国外标准(美国环保署的《美国饮用水水质标准》、欧盟的《饮用水水质指令》、世界卫生组织的《饮用水水质准则》及其他某些国家的饮用水标准)中的微生物指标进行了分析比较。综合评价了各水质标准的特点,并对我国的水质标准的微生物指标提出了意见和建议。  相似文献   

3.
2012年1月—2013年12月在鸭河口水库设南河店、安庄、库心、坝下4个监测站,在其下游白河盆窑段面设1个监测站,进行水质的生物监测和理化监测。按水样采集标准方法采样,采用营养状态指数法和污水生物系统法对鸭河口水库水质进行营养状态评价。结果表明,浮游藻类有7门40科90属208种(含变种),其硅藻、绿藻和蓝藻所占的百分比依次为57.79%、23.60%和11.62%;浮游藻类污染指示种5门19科25属36种(含变种),中污型藻类指示种占污染指示种的80.56%;浮游动物有28科30属36种(含变种),仅检出1种β-中污型指示种;除总氮超标外,其他理化指标和微生学指标均符合Ⅰ类水质标准。综合评定,南阳市饮用水水源区水质处于中营养状态。  相似文献   

4.
环境监测是水生态健康监测与评估的重要环节,基于物理、化学监测的传统水质监测通常仅能提供独立的数据信息,不能全面、直观地反映水环境状况。基于生物等生命体导向的水生态监测通过生物对环境的响应,能够直接反应复杂水体状况,在水环境健康监测与评估中占据重要地位。基于病原微生物、指示生物介绍了生物监测中的常规生物指标,总结了包括藻类、无脊椎动物和鱼类在内的常见指示生物在不同类型污染水体中的环境指示作用。从生物毒性效应出发介绍了常用的毒性效应测试方法、分析了污染物在不同生物学水平的响应,从而指明生物毒性效应在水环境健康评估中的发展优势。再从生态完整性角度阐述了生态完整性评价的一般方法和新兴分子生物学技术在水生态健康评估中的应用。重点指出环境毒理学和分子生物学在水环境监测的优势,以期为更加科学精确地进行水生态健康监测预警提供支撑。  相似文献   

5.
生态环境监测人员在工作中面临病原微生物安全风险。调研发现,已颁布的相关法律法规、标准和规定,多侧重于实验室及医务人员的病原微生物安全防护,缺少生态环境监测人员在采样、样品运输和交接、废弃物处置等过程中的病原微生物安全规范,新冠肺炎疫情期间的防护指导文件比较笼统。笔者在已颁布的法律法规和各项标准基础上,识别和评估生态环境监测全过程病原微生物安全风险,提出相应的监测要求和人员防护建议,针对采样、样品运输和交接、实验室分析和废弃物处置过程提出个人防护措施及突发情况下的应急防护措施。  相似文献   

6.
细菌在近海污染监测及评价中的应用研究   总被引:1,自引:0,他引:1  
通过对天津渤海湾的三个河流入海口(青静黄排水河、独流减河和海河),不同季节实地采集的水样中微生物数量及理化因子如温度、盐度、溶解氧(DO)、化学需氧量(COD)、无机氮等的研究,发现细菌指标与理化指标对污染指示结果有一定的相似性,都指示青静黄排水河河口污染较严重.利用SPSS软件的相关性分析方法对实验结果进行分析,发现水环境中细菌指标与理化因子具有一定的相关性.其中异养菌数与COD呈显著正相关性(P<0.05),与DO呈负相关性(P=0.051);大肠菌群数与理化因子DO呈极显著负相关关系(P<0.01),与COD呈正相关关系(P=0.061),与NO3-N,盐度呈负相关性(P>0.05).其他细菌指标与各理化因子也存在一定的相关性,但不明显.  相似文献   

7.
杭州西湖水体粪大肠菌的监测及环境意义   总被引:4,自引:0,他引:4  
粪大肠菌群指数是综合评价城市污水,尤其是生活污水污染的一个重要指标.作者于1999.1~1999.7时杭州西湖水体的FC进行了监测,结果表明西湖受到了生活污水的污染,特别是三条入湖溪流污染程度十分严重.三条溪流的FC指数超出我国地表水Ⅲ类标准的10倍.水体中FC指数同TN、TP浓度呈高度正相关.  相似文献   

8.
对我国《地表水环境质量标准》(GB 3838-2002)和《地下水质量标准》(GB/T 14848-1993)与美国EPA《清洁水法案》和《安全饮用水法案》中适用范围、分类分级、指标项目、标准限值以及修订周期等方面内容进行比较分析。指出了我国饮用水水源水质标准存在的问题,提出构建和修订我国水源地水质标准的建议。  相似文献   

9.
为了提升扬州市公共场所卫生状况和传染病防控能力,按照国家标准于2019年对扬州市宾馆(酒店)类、沐浴类、理发店类、美容店类和候车室类5类公共场所的健康危害因素(空气质量和公共用品用具)进行监测,按照《公共场所卫生指标及限值要求》(GB 37488—2019)进行评价。结果表明,共监测空气质量样品216份,合格率为84.26%,共监测公共用品用具1 414份,合格率为96.46%,不同场所的空气质量合格率和公共用品用具微生物指标合格率差异均具有统计学意义(P 0.05)。不同类型公共用品用具监测项目中,金黄色葡萄球菌的合格率最高(100%),而真菌总数的合格率最低(93.75%)。各类公共用品用具中,拖鞋合格率最低(91.67%),床单相对较高(99.70%)。扬州市公共卫生状况总体良好,在空气质量监测中,物理项目和微生物项目合格率较低,在公共用品用具监测中,细菌总数和大肠菌群存在不合格现象。  相似文献   

10.
深圳市政污水排海工程排污混合区范围的确定   总被引:6,自引:0,他引:6       下载免费PDF全文
应用全场数学模型模拟了深圳排海的市政污水中悬浮物、化学需氧量、无机氮、活性磷酸盐及大肠菌群5个污染因子在海域大潮和小潮时对海域面积的影响。指出在不同水文条件和23m^3/d排放量下,不同质量浓度的活性磷酸盐和大肠菌群超过Ⅲ类海水水质标准的影响面积在大潮时分别为0.04km^2和7.72km^2,小潮时分别大于0.08km^2和6.16km^2;无机氮由于海水本底值较高,使得这一指标在全海域内超过Ⅲ类海水水质标准;而化学需氧量和总悬浮物质量浓度符合Ⅲ类海水水质标准。通过将现场监测资料用数模计算,提出排海污水混合区面积为1.5km^2。同时还引用部分国外的计算公式,对几种混合区允许范围进行了估算。  相似文献   

11.
Rodrigo de Freitas Lagoon is an urban ecosystem undergoing accelerated degradation, therefore selected as a model for microbiological quality studies of tropical lagoons. The aim of this study was to evaluate the abundance and the spatial distribution of fecal pollution indicators and pathogenic microorganisms in the lagoon. The relationships between microbial groups and abiotic measurements were also determined to evaluate the influence of environmental conditions on bacterial distribution and to identify the capability of coliforms and Enterococcus to predict the occurrence of Vibrio, Staphylococcus aureus, and Salmonella. Surface water samples were collected monthly, from December 1999 to October 2000. Analyses were performed by traditional culture techniques. A uniform spatial distribution was observed for all bacterial groups. The fecal pollution indicators occurred in low abundances while potentially pathogenic microorganisms were consistently found. Therefore, our study supported the use of counts of coliforms and Enterococcus to indicate only recent fecal contamination.  相似文献   

12.
Water quality monitoring is essential for the provision of safe drinking water. In this study, we compared a selection of fecal indicators with universal Bacteroidales genetic marker to identify fecal pollution of a variety of drinking water sources. A total of 60 samples were collected from water sources. The microbiological parameters included total coliforms, fecal coliforms, Escherichia coli and fecal streptococci as the fecal indicator bacteria (FIB), Clostridium perfringens and H2S bacteria as alternative indicators, universal Bacteroidales genetic marker as a promising alternative fecal indicator, and Salmonella spp., Shigella spp., and E. coli O157 as pathogenic bacteria. From 60 samples analyzed, Bacteroidales was the most frequently detected indicator followed by total coliforms. However, the Bacteroidales assay failed to detect the marker in nine samples positive for FIB and other alternative indicators. The results of our study showed that the absence of Bacteroidales is not necessarily an evidence of fecal and pathogenic bacteria absence and may be unable to ensure the safety of the water. Further research, however, is required for a better understanding of the use of a Bacteroidales genetic marker as an indicator in water quality monitoring programs.  相似文献   

13.
In 2010, a magnitude 7.0 earthquake struck Haiti, severely damaging the drinking and wastewater infrastructure and leaving millions homeless. Compounding this problem, the introduction of Vibrio cholerae resulted in a massive cholera outbreak that infected over 700,000 people and threatened the safety of Haiti’s drinking water. To mitigate this public health crisis, non-government organizations installed thousands of wells to provide communities with safe drinking water. However, despite increased access, Haiti currently lacks the monitoring capacity to assure the microbial safety of any of its water resources. For these reasons, this study was designed to assess the feasibility of using a simple, low-cost method to detect indicators of fecal contamination of drinking water that could be implemented at the community level. Water samples from 358 sources of drinking water in the Léogâne flood basin were screened with a commercially available hydrogen sulfide test and a standard membrane method for the enumeration of thermotolerant coliforms. When compared with the gold standard method, the hydrogen sulfide test had a sensitivity of 65 % and a specificity of 93 %. While the sensitivity of the assay increased at higher fecal coliform concentrations, it never exceeded 88 %, even with fecal coliform concentrations greater than 100 colony-forming units per 100 ml. While its simplicity makes the hydrogen sulfide test attractive for assessing water quality in low-resource settings, the low sensitivity raises concerns about its use as the sole indicator of the presence or absence of fecal coliforms in individual or community water sources.  相似文献   

14.
Concern over the presence of fecal coliform in public drinking water supplies has been expressed in recent years in Pakistan since it has been regarded as pathogenic organism of prime importance in gastroenteritis. Two major drinking water distribution systems in the Cantt area of Rawalpindi district covering the Westridge and Tench areas was monitored over a 2-month period to determine the prevalence of fecal coliform and chlorine residual. The collected samples were examined for total chlorine, free chlorine residual, chloramines, total coliforms, fecal coliforms, and turbidity. The drinking water quality monitoring in the distribution network was performed by collecting samples from water source, overhead reservoir, and residential taps. In the Westridge area, total chlorine varied from the lowest value of 0.27 mg/L at Station # W-5 to the highest value of 0.42 mg/L at Station # W-2, total coliforms varied from 1.1 to 3.6 most probable number (MPN)/100 mL with presence of Escherichia coli in all samples, total dissolved solids (TDS) ranged from 199.5 to 205 mg/L, conductivity fluctuated between 399 and 411 microS/cm, and turbidity varied from 0.43 to 0.73 NTU. In the Tench area, the value of total chlorine ranged from 0.14 mg/L at Station # T-7 to 0.55 mg/L at Station # T-1. Total coliform varied from 3.6 to 5.1 MPN/100 mL and fecal coliform were detected at all the stations except at Station # T-1. TDS ranged from 201.4 to 257 mg/L, conductivity varied from 343 to 513 microS/cm, and turbidity ranged between 0.66 and 1.55 NTU. It is recommended to the respective agencies to ensure that the chlorine residual is available at consumer end.  相似文献   

15.
伴随着日本经济的高速发展,其水环境污染问题十分严峻,防治水污染成为日本政府在20—21世纪的重要工作之一。经过几十年的努力,日本水污染治理取得长足进展。目前日本水环境监测已经形成由水和土壤等方面组成的水循环监测体系,包括地表水、近海、湖泊、地下水和土壤等。在日本的重要环保法律法规中均有涉及水环境监测工作内容的明确法律条文,国家行政机关、地方政府和公立的科研单位在具体实施水环境质量监测过程中的职责和义务不同,水环境质量监测管理的国家行政机关以环境省为主,地方政府根据环境省制定的水环境质量标准、监测技术方法及规范,制定所辖地域的监测方案并实施监测,公立的环境科研机构在环境质量监测工作中起到重要的支撑作用。笔者概述了日本开展水环境质量监测的法律依据和监测管理情况等。  相似文献   

16.
Natural spring water has unique properties, as it is rich in minerals that are considered to be beneficial to human health. A survey of the microbiological quality of natural spring water was conducted to assess possible risks from the consumption of the water by visitors in recreational mountain areas located in Seoul, South Korea. The densities of total coliforms and Escherichia coli were measured during the spring and the summer of 2002 to investigate the presence of coliform bacteria in the drinking spring waters. Total coliforms were detected in all samples and the mean density of total coliforms was up to a maximum of 228 CFU/mL. Detectable E. coli was found in 78% of all samples and the mean densities of E. coli varied from a minimum of 0 CFU/mL to a maximum of 15 CFU/mL in all samples. Malfunctioning septic systems and wildlife population appear to be the main source of E. coli contamination. Presence of E. coli in natural spring water indicates potential adverse health effects for individuals or populations exposed to this water. The fecal contaminated spring water may present an unacceptable risk to humans if it is used as raw drinking water.  相似文献   

17.
A regional survey of the microbiological water quality along the shoreline of the Southern California Bight (SCB), from Point Conception south to Ensenada, Mexico, was conducted during August, 1998, by 36 agencies under the coordination of the Southern California Coastal Water Research Project (SCCWRP). Microbiological water quality was assessed by calculating the percentage of shoreline-mile-days that exceeded bacterial indicator thresholds for total and fecal coliforms, total/fecal ratios, and enterococci. Sample sites were selected using a stratified random sampling approach, with the SCB recreational shoreline divided into six strata: high- and low-use sandy beaches, high- and low-use rocky shoreline, and perennial and ephemeral freshwater outlets. Samples were collected on a weekly basis at a total of 253 sites, beginning on August 2nd, 1998 and continuing for five weeks. Samples were analyzed by 22 participating labs using their normal methods (multiple tube fermentation, membrane filtration, Colilert® and/or Enterolert®). All labs met testing criteria established through intercalibration exercises and quality control check samples distributed during the sampling period. Nearly 95% of the shoreline-mile days did not exceed daily and monthly bacterial indicator thresholds, demonstrating good bacteriological water quality along the SCB shoreline. Freshwater outlets, comprised mainly of storm drains, had the poorest water quality with 60% and 40% of the shoreline-miles exceeding monthly and daily thresholds, respectively. Freshwater outlets were also more likely to demonstrate exceedances by multiple indicators at a single site, and repeat exceedances at sites over the five-week period. Compared with the southern California beaches, Mexican beaches had nearly 5 times the number of exceedances for total and fecal coliforms, and nearly 8 times the number of exceedances for total/fecal ratios.  相似文献   

18.
随着环境应急事故处理的规范化要求不断深入,在水污染事件应急监测的不同阶段应做好相关质量控制工作。研究以某突发水环境污染事件为例,探讨跟踪监测阶段分析质量控制措施,包括实验室手工分析和采用自动分析设备分析。质量控制数据统计结果表明:该污染事件跟踪监测期间实验室分析数据处于可控状态,自动分析仪器所得数据与实验室分析数据可比。案例结果表明:在确定主要污染物后的污染事件跟踪监测期内,实验室分析应严格按照相关标准规范要求开展质量控制。建议在水污染事件应急监测中,通过方法比对和规范的质量控制措施,使监测处于受控状态,进一步提高应急监测数据质量,保证监测结果的及时、科学、准确和可靠。  相似文献   

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