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1.
基于16S rRNA和18S rRNA高通量测序技术,研究了长江南京段21条主要入江支流不同水期底质多营养级微生物的群落结构特征,利用Shannon指数等分析了样品中微生物群落的多样性。结果表明,42个样品的细菌群落归属于285个纲水平上的OTUs (operational taxonomic units),归属于62个门;优势菌门为变形菌门(Proteobacteria)、酸杆菌门(Acidobacteria)、绿弯菌门(Chloroflexi)等,优势菌纲为β-变形菌纲、α-变形菌纲、δ-变形菌纲等。原生动物中的优势类群为不等鞭毛类(Heterknta)、囊泡虫类(Alveolata)和绿色植物(Chloroplastida),后生动物中的优势类群为节肢动物(Arthropoda)、轮虫(Rotifera)和线虫(Nematoda)。平水期底质的细菌、原生及后生动物的多样性相对较低,入江支流的水生态系统相对不稳定,尤其是金川河、七里河等。  相似文献   

2.
主要采用等标污染负荷、营养状态指数、生物综合毒性指标和GIS手段,通过对秦皇岛海域3个陆源入海排污口及邻近海域水质因子调查,考察典型陆源入海排污对邻近海域富营养化及生物毒性的影响。结果表明,大蒲河、人造河和洋河入海污染物的等标污染负荷比分别为36.9%、61.1%和2.0%,其中大蒲河和人造河对该海域污染负荷比的贡献达98%,为主要污染源;邻近海域主要超标污染物为PO3-4和DIN,PO3-4浓度与NQI相关分析(r=0.76,P=0.004 6)和GIS空间对比表明,PO3-4可能是造成该海域富营养化的主要因子。NQI和发光细菌发光抑制率的空间分布特征表明,该海域受陆源排污的影响比较明显。  相似文献   

3.
在兰州市不同室外环境区域布设4个采样点,使用PSW-6型安德森(Andersen)6级筛孔撞击式空气微生物采样器采集大气细菌气溶胶,分析其浓度和粒径日变化特征。结果表明,不同季节各采样点的大气细菌气溶胶浓度上午时段为88 CFU/m3~1 335 CFU/m3,下午时段为78 CFU/m3~865 CFU/m3。除了夏季兴隆山上午时段外,其余不同季节各采样点上、下午时段的大气细菌气溶胶粒径均主要分布于Ⅰ级—Ⅳ级(>2.10μm),占比为 70.54%~93.94%;Ⅴ级(1.10 μm~2.10μm)和Ⅵ级(0.65 μm~1.10 μm)大气细菌粒子占比较低,仅为6.06%~29.46%。  相似文献   

4.
椒江口海域沉积物微生物群落及其对环境因子的响应   总被引:1,自引:0,他引:1  
微生物群落在有机污染物生物地球化学循环中起着十分关键的作用。微生物群落结构和多样性及其变化在一定程度上反映了土壤的质量。宏基因组学能够在整体水平解析微生物群落结构,真实地揭示原位环境中微生物群落的复杂性和多样性。应用Illumina Mi Seq宏基因组高通量测序技术,在椒江口海域沉积物中鉴定得到古菌和细菌共33门类,变形菌门为明显的优势门类,其序列数占总数的62.1%。其他较为丰富的门类包括绿弯菌门、放线菌门、拟杆菌门。采用mothur软件计算Ace、Chao、Shannon、Simpson指数,结果发现,Ace、Chao的均值分别为511、518,Shannon在5.0左右,Simpson接近于0.02,显示出海域沉积物中微生物多样性较高且差异较小。通过微生物门类多样性与环境因子的相关性分析发现,PCB101、盐度和γ-氯丹对微生物门水平的群落分布具有显著性影响。计算微生物门类组成与环境因子间Spearman相关系数并绘图分析,温度、盐度、pH与多个微生物门类的组成和分布呈现明显的相关关系。  相似文献   

5.
南黄海2007—2017年浮游植物群落结构及多样性变化   总被引:3,自引:0,他引:3  
在2007-2017年秋季,利用专业海洋调查船对整个南黄海海域开展了5个航次的浮游植物调查。结果发现:调查海域共有浮游植物371种,隶属4门108属。其中硅藻门有56属237种,甲藻门有24属98种,硅藻和甲藻是组成南黄海海域浮游植物最主要的两大类群。主要优势种有裸甲藻(Gymnodinium sp.)、柔弱拟菱形藻(Nitz.delicatissima)、蓝隐藻(Chroomonas sp.)、菱形海线藻(Thalassionema nitzschioides)、锥状施克里普藻(Scrippsiella trochoidea)和环沟藻(Gyrodinium spp.)等。2007年以来,南黄海浮游植物种类数呈明显上升趋势。浮游植物细胞密度平均为1.96×104个/L,年际变化较大。将调查结果与众多文献比对,共发现9种藻类为中国新记录藻种,其中有4种硅藻和5种甲藻。  相似文献   

6.
浅谈监测数据审核及异常数据的分析与判断   总被引:3,自引:0,他引:3  
1异常数据的判断与分析发现和判别异常数据,首先要用污染时空分布及其变化来进行判断,如排污口污染物浓度低于远离污染源地区的浓度,污染物浓度明显超越常年水平,浓度时空分布出现反常现象等。因此,采样时要对采样点周围环境状况、气象条件、样品感观及样品采集等情...  相似文献   

7.
采用投加悬浮填料方法和高通量测序技术研究新疆干旱寒冷地区污水处理厂的硝化、反硝化速率及生物膜菌群。结果表明,环境温度与水温具有良好的相关性;悬浮填料对氨氮和NO-3-N均有降解作用,平均去除率分别为75.72%和81.42%;悬浮填料填充率在20%~30%、曝气量在6.0 m3/h~6.5 m3/h时,总氮平均去除率为75%。变形菌门和拟杆菌门为生物膜的优势菌,绿弯菌门和黄杆菌门与污泥膨胀有关,装甲菌门是导致脱氮不佳的细菌。  相似文献   

8.
养马岛后海区域秋季大型底栖动物群落特征   总被引:1,自引:0,他引:1  
2010年9月在烟台养马岛后海海域开展了大型底栖动物调查,并进行了群落特征分析。结果表明,该海域内的大型底栖动物多毛类占绝对优势,群落间差异较小,可划分为4 个群落,长叶索沙蚕(Lumbrinereis longiforlia)、钩毛虫(Sigambra sp.)、中蚓虫(Mediomastus sp.)等多毛类生物的丰度高低是构成群落差异的主要因素。栖息环境的差异是造成群落间差异的主要原因,Shannon-Wiener多样性指数为1.17~2.92,平均值为2.13。人为扰动是影响多样性水平的主要因素,在航道通过、养殖区分布的影响下,群落受到不同程度的扰动,整体稳定性较弱。  相似文献   

9.
为了探究浮游细菌和蓝藻暴发之间的关系,利用实时荧光定量PCR和高通量测序技术,对夏季蓝藻暴发期间太湖竺山湾表层水和底泥中浮游细菌群落结构和多样性进行研究。结果表明,从门水平来看,水样和底泥中平均相对丰度最高的为变形菌门,放线菌门次之,此外蓝藻门也有一定的比例,可为水华暴发提供预警指示;从属水平来看,水样中的优势细菌主要为GpXI和GpIIa,底泥中为Gp6和GpIIa。  相似文献   

10.
利用高通量测序技术,选择16S rRNA V6区作为目标片段,对夏季太湖梅梁湾水体中的细菌群落结构进行了分析,结果表明,共产生了101 427条优质序列,细菌为100 935条,占99.5%;在蓝藻暴发期间,共检测到14门,55属,610个操作分类单位。优势门类为蓝藻门(39.7%),放线菌门(27.2%)和变形菌门(23.4%),微囊藻属(21.0%)和聚球藻属(15.9%)为主要优势种。  相似文献   

11.
Airborne transmission of bacterial pathogens from point sources (e.g., ranches, dairy waste treatment facilities) to areas of food production (farms) has been suspected. Determining the incidence, transport and viability of extremely low levels of pathogens require collection of high volumes of air and characterization of live bacteria from aerosols. We monitored the numbers of culturable bacteria in urban aerosols on 21 separate days during a 9 month period using high volume cyclonic samplers at an elevation of 6 m above ground level. Culturable bacteria in aerosols fluctuated from 3 CFU to 6 million CFU/L of air per hour and correlated significantly with changes in seasonal temperatures, but not with humidity or wind speed. Concentrations of viable bacteria determined by fluorescence staining and flow cytometry correlated significantly with culturable bacteria. Members of the phylum Proteobacteria constituted 98% of the bacterial community, which was characterized using 16S rRNA gene sequencing using DNA from aerosols. Aquabacterium sp., previously characterized from aquatic environments, represented 63% of all clones and the second most common were Burkholderia sp; these are ubiquitous in nature and some are potential human pathogens. Whole genome amplification prior to sequencing resulted in a substantial decrease in species diversity compared to characterizing culturable bacteria sorted by flow cytometry based on scatter signals. Although 27 isolated colonies were characterized, we were able to culture 38% of bacteria characterized by sequencing. The whole genome amplification method amplified DNA preferentially from Phyllobacterium myrsinacearum, a minor member of the bacterial communities, whereas Variovorax paradoxus dominated the cultured organisms.  相似文献   

12.
于2015—2016年在秦山岛海域开展4个航次的大型底栖动物调查,用优势度指数法和多样性指数法对底栖动物优势种和多样性状况进行评价。结果显示,四季共采集鉴定底栖动物7门69种,其中环节动物25种,软体动物19种,节肢动物16种,棘皮动物3种,脊索动物、纽形动物和腔肠动物各2种;优势种共14种。大型底栖动物种数和密度均值均为春季>秋季>夏季>冬季,生物量均值为夏季>冬季>春季>秋季,密度和生物量的空间分布无明显规律。多样性评价结果表明,秦山岛海域底栖动物群落受到轻度干扰,底栖环境处于轻度污染状态;除人为干扰和环境因子外,种间关系及生活史特征也是影响底栖动物群落结构变化的重要因素。  相似文献   

13.
Soil microbial ecosystems are responsive to environmental changes that underpin the biological functions of the soil. The present study was conducted to profile variations in the microbial ecological system of remediated soil (R) and petroleum contaminated soil (P) based on comparisons with soil that had not been contaminated (N), using a cloning library of taxonomic genes (16S rRNA gene for bacteria and 18S rRNA gene for eukaryotes) and functional genes (nifH, amoA and narG). The results showed that N and R had a similar distribution in both the taxonomic genes and functional genes for bacteria and eukaryotes, which were dominated by Proteobacteria and Arthropoda, respectively. Phylogenetic analysis based on the nifH gene showed that the sequences from the three soils were clustered into six taxonomic groups, Actinobacteridae, and Alpha-, Beta-, Gamma- and Delta-proteobacteria, as well as an unclassified group. Evaluation of the amoA gene revealed that all sequences derived from the three samples belonged to Betaproteobacteria. The R and N soil had similar Shannon-Wiener diversity index (H') values, both of which were significantly higher than that of the P soil. The most abundant bacterial phylotype identified in the N and R soils were the same and were related to an uncultured bacterial clone (GAN-SB17, FN423475). None of the narG genes were found in the P soil. Similar results in terms of distribution, composition and the related index were obtained for nifH and amoA. These parameters may comprise a biological ecology index that may be applied to aid the design, implementation and evaluation of soil bioremediation.  相似文献   

14.
近年来,空气污染已成为长三角地区最关键的环境问题之一,气溶胶颗粒物(PM)是最主要的污染物之一。生物气溶胶作为颗粒物的重要组成部分,可能对空气质量和人体健康产生不利影响。利用高通量测序方法研究了江苏省泰州2019年11月至2020年1月期间发生沙尘和霾污染时生物气溶胶中细菌群落结构组成特征。结果表明:冬季沙尘天和霾天气溶胶细菌群落结构及多样性呈现不同的变化特征,霾天细菌群落多样性、均匀度以及基于进化的多样性明显高于夏季优良天,粒径差异增大;沙尘天细菌群落结构变化和霾天正好相反,不仅大气微生物丰富度会降低,细菌群落结构的多样性和均匀度也会降低。优良天粗细颗粒的细菌群落组成和优势种相似度较高,沙尘和霾污染天粗细颗粒间的细菌组成差异较大,病原细菌的种类和相对丰度也明显增加,优势类群中致病菌浓度上升的主要有假单胞菌属(Pseudomonas)、雷氏菌属(Ralstonia)、肠球菌属(Enterococcus)、拟杆菌属(Bacteroides)、埃希氏菌-志贺氏菌属(Escherichia-Shigella)、链球菌属(Streptococcus)等,增加了人类、动物及农业生产活动潜在的暴露风险。基于丰度热图分析污染天各粒径的细菌群落结构相似性,沙尘天大部分粒径聚在一起,粒径间的细菌组成相似性较高;霾天样品的粒径分布总体较分散,随着污染加重,粒径差异有增大的趋势。  相似文献   

15.
为了解永定河流域大型底栖动物群落结构与空间分布状况,2017年春季调查了永定河流域大型底栖动物群落,采集并鉴定出大型底栖动物77个分类单元,其中水生昆虫为绝对优势类群。聚类分析表明,永定河流域底栖动物群落结构在空间上与流域地理格局基本一致,上游区洋河、妫水河、桑干河以直突摇蚊、流水长跗摇蚊、间摇蚊为优势类群,中游区永定河山峡段以钩虾、台湾蜉、近岸细蜉为主,下游区五湖一线段以德永摇蚊、恩菲摇蚊、沼虾为优势类群。直接收集者成为流域内绝对优势功能摄食类群,在不同区域的相对丰度均达到90%以上。单因素方差分析显示,Shannon-Wiener多样性指数和Pielou均匀度指数无组间差异,Margalef丰富度指数和物种数在中游区和下游区差异显著(P0.05)。研究表明流域大型底栖动物群落出现了整体退化,生物多样性下降,重度-中度耐污类群成为优势,多数河段功能摄食类群不完整,永定河流域生态系统保护和修复工作亟待开展。  相似文献   

16.
源头区溪流是河流生态系统最脆弱的部分,也是淡水底栖大型无脊椎动物(简称底栖动物)集中分布的热点区域,在流域物种库的形成和生态系统的多样性维持过程中具有不可忽视的作用。然而,目前国内对源头区溪流底栖动物多样性的系统调查研究较少。笔者对位于浙江省丽水市瓯江源头区的龙泉溪进行了底栖动物多样性调查研究,分别于2021年丰水期和平水期在覆盖龙泉溪主要山溪河流的18个样点采样,共采集获得底栖动物标本3 700余号。通过联合使用传统形态分类和DNA条形码技术,共鉴定出底栖动物165种,隶属5门、9纲、19目、68科、124属。联合使用DNA条形码可使底栖动物科、种水平的分辨力提升28.3%和34.1%。调查研究表明:龙泉溪底栖动物多样性丰富,物种组成以节肢动物为主(占比高达87.9%,145种),其中水生昆虫占绝大多数(共计8目、48科、140种,占84.8%),主要优势种为鞘翅目的狭溪泥甲属1种Stenelmis sp.1、毛翅目的纹石蛾属Hydorpsyche和短脉纹石蛾属Cheumatopsyche各1种;底栖动物群落多样性丰富,且在丰水期和平水期均维持在较高的水平;海拔和底质类型等微生境条件对底栖动物多样性分布的影响分析显示,底质类型对底栖动物分布密度的影响显著。对水生态状况的生物评估结果显示:Biotic Index污染生物指数更适合龙泉溪流域的水质健康状况评估,龙泉溪流域整体的水生态状况健康,水质属清洁或极清洁。该研究使用传统形态学和DNA条形码技术相结合的鉴定方式,提高了底栖动物物种鉴定的精度,所获得的多样性调查和水质评价结果为瓯江源头区溪流乃至整个流域的生态保护和长期监测提供了本底基础资料。  相似文献   

17.
Airborne bacteria emissions from oxidation ditch with rotating aeration brushes were investigated in a municipal wastewater treatment plant in Beijing, China. Microbial samples were collected at different distances from the rotating brushes, different heights above the water surface, and different operation state over a 3-month period (April, May, and June) in order to estimate the seasonal variation and site-related distribution characteristics of the microorganisms present. The concentration of bacterial aerosol was analyzed by culture methods, while their dominant species, genetic structure and diversity were assayed using bio-molecular tools. Results showed that total microbial concentrations were highest in June and lowest in April. The mechanical rotation caused remarkable variation in concentration and diversity of culturable airborne bacteria before and after the rotating brushes. The highest concentration was observed near the rotating brushes (931?±?129–3,952?±?730 CFU/m3), with concentration decreasing as distance and height increased. Bacterial community polymerase chain reaction and denaturing gradient gel electrophoresis indicated that diversity decreased gradually with increasing height above the water surface but remained relatively constant at the same height. All dominant bacteria identified by DNA sequence analysis belonged to Firmicutes. Pathogenic species such as Moraxella nonliquefaciens and Flavobacterium odoratum were isolated from the bioaerosols. Due to the serious health risks involved, exposure of sewage workers to airborne microorganisms caused by brush aerators should be monitored and controlled.  相似文献   

18.
An investigation of bacteria found in rubber processing wastes was carried out. Rubber wastes which include effluents from washing tanks and natural rubber waste serum (NRWS) were obtained from Greenpark Rubber Industries Limited, Umutu, Delta State, Nigeria. Five bacterial species were isolated from the wastes. These include Arthrobacter sp., Bacillus sp., Lactobacillus sp., Psuedomonas sp. and Streptococcus sp. Apart from these a number of coliforms were also encountered. Arthrobacter sp. was found to be the dominant species and its potential to utilize rubber hydrocarbon was determined. It was found that the growth of Arthrobacter in both effluent and NRWS was related to pH with the highest growths recorded at pH of 8.5 and 7.5 for effluent and NRWS respectively. It was also found that at controlled pH of 7.5 in NRWS, the growth of Arthrobacter was consistent and was accompanied by a reduction in biological oxygen demand (BOD) which was the the main index for measuring pollution strength of the wastes. It is therefore being recommended that rubber wastes be treated with Arthrobacter under controlled pH to reduce their pollution potentials before disposal. It is however suggested that a combined biological treatment using both Arthrobacter and Mucor as was earlier suggested be used. It is also recommended that chemical flocculants should be used to remove suspended solids in the effluent. A combination of these two cheap methods will go a long way in alleviating the problems of pollution caused by rubber effluents from some tropical rubber processing factories.  相似文献   

19.
东平湖是南水北调东线工程的重要调蓄湖泊,在防洪、灌溉、休闲旅游、水产养殖及水资源供应等方面发挥着至关重要的作用。为了解南水北调东线调水后的东平湖浮游植物群落结构及水体健康状态,于2016年4月(春季)、7月(夏季)、10月(秋季)及2017年1月(冬季)对东平湖不同空间区域(根据调水路线划分为进水区、湖中区和出水区)的浮游植物进行了调查研究,共检出浮游植物8门143种(属),其中,绿藻门种类数最多,蓝藻门次之,硅藻门位居第三。东平湖浮游植物密度呈现出显著的季节差异,秋季(1.65×108cells/L)、夏季(3.06×107cells/L)浮游植物密度明显高于春季(1.68×106cells/L)和冬季(1.27×106cells/L),而出水区、湖中区及进水区之间未呈现出显著的区域差异,年均密度为4.96×107cells/L。浮游植物优势种群共计14种(属),以蓝藻门的伪鱼腥藻(Pseudoanabaena sp.)、拟浮丝藻(Planktothricoides sp.)为主。采用Shannon-Wiener多样性指数、Pielou均匀度指数、Marglef丰富度指数及优势种评价法对东平湖进行的水质生物评价表明,东平湖水质总体处于中污染水平。与历史数据相比,调水后的东平湖浮游植物群落密度、物种多样性均高于调水前,且主要优势种以β-中污染指示种居多,说明东平湖整体水质情况较调水前有所改善。  相似文献   

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