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1.
A serious forest decline of Betula ermanii Cham. has been observed at Mt. Mae-Shirane, Oku-Nikko, Japan, where high ozone (O3) concentration and severe water deficiency have been measured. In order to consider the possibility whether O3 and/or water stresses could have been the causes of the forest decline of B. ermanii, plant growth experiments were conducted in environment-controlled growth cabinets. Two-year-old seedlings of B. ermanii were exposed to either charcoal-filtered air (O3 concentration <5 ppb) or 50 ppb O3 (daily average, ranging between 20–100 ppb) for 123 days at 20.0/12.5 ± 1.0°C (day/night) and 70/80 ± 7% relative humidity (day/night). Simultaneously, seedlings were treated with three watering regime: 1.0 < pF < 1.8 (no water stress), 1.8 < pF < 2.5 (mild water stress) or 2.5 < pF < 3.0 (severe water stress). O3 exposure significantly reduced the dry weights of leaf, root and the whole plant, while water stress significantly reduced the dry weights of each organ and the whole plant. Significant reductions of net photosynthesis, transpiration and stomatal conductance were also observed under O3 and/or water deficiency treatments, while contents of RuBP carboxylase/oxygenase (Rubisco), chlorophyll a+b and some essential nutrient elements (N, P, K, Mg and Ca) were not markedly changed. It was suggested that the decrease in net photosynthetic rate induced mainly by stomatal closure was the major cause of the growth reduction under O3 and/or water stresses. No significant interactions between O3 and water stresses were observed in terms of the depression of dry matter production, which suggested that simultaneous stress treatments of O3 exposure and water deficiency could affect the tree growth of B. ermanii additively.  相似文献   

2.
Pasture selection by livestock is an essential topic for rangeland management, especially in trace element-contaminated soils. We have studied the composition (nutrients and trace elements) of a grass-based diet from soils affected by a mine spill at different growth stages (October 2008 to May 2009). A diet based on other plants (mainly Compositae species) was also studied (May 2009) for comparison. Faeces and mane hair of horses feeding on these pastures were also analysed. Micronutrient (Cu, Fe, Mn and Zn) and potentially toxic trace element (As, Cd, Pb, Tl) concentrations were below the maximum tolerable levels (MTL) for horses, except for Fe (at early growth of pastures) and Cd (in the diet based on ‘other’ plants). Values of potential ingestion of Fe by horses were higher than 10 mg kg body weight − 1 day − 1. Cadmium concentrations in some pasture samples (those composed of Compositae species) were higher than 3 mg kg − 1. Potential toxicity of such Cd levels in pastures is uncertain, since a high disparity of criteria about MTL by cattle exists (between 0.5 and 10 mg kg − 1 diet). Nutrient concentrations were adequate for horses, which could counteract possible harmful effects derived from trace element ingestion. The analyses of excreta and mane hair point to the low risk of toxicity derived from the consumption of these contaminated pastures. However, the ingestion of regenerating pastures (autumnal samples) should be avoided due to the greater risk of ingestion of contaminated soil attached to the plant material. Management of these pastures by grazing requires periodic monitoring. Special attention should be given to Fe and particularly Cd (non-essential element) which accumulates in animal organs, where it could provoke uncertain long-term effects.  相似文献   

3.
Delta-aminolevulinate dehydratase (ALAD) activity has been used in prokaryotes and eukaryotes as a biomarker for environmental lead (Pb) exposure and toxicity. Microorganisms are sensitive indicators of toxicity at the fundamental level of ecological organization, but bacterial biomarker studies are focused on the Pseudomonas strains in Group I and E coli. The objectives of the present work were to determine if Burkholderia gladioli belonging to group II, due to its 16SrRNA similarity, can be used as biomarker in metal contamination and compare its possible usage with Pseudomonas aeruginosa and Citrobacter freundii (previously known as Esherichia freundii) and Bacterium freundii which are classified in Group I. In this study, ALAD activity in an environmental strains of Burkholderia gladioli, Pseudomonas aeruginosa, Citrobacter freundii were investigated to evaluate potential inhibition by Pb and other toxic metals. When the ALAD activity of Burkholderia gladioli was tested, Co and Pb decreased activity by 27 and 71%, respectively. In addition to these findings, Zn increased the activity up to 26%. These effects were found to be statistically meaningful (p < 0.05). It was determined that the increase of lead concentration inhibites the ALAD activity at each of the three strains. There was a statistically significant dose–response relationship between ALAD activity in cells of Burkholderia gladioli and Pb (Pearson correlation coefficent = −0.665; r 2 = 0.665, and p < 0.001). The strongest ALAD inhibition which was measured was 90% at Burkholderia gladioli when protein extracts were incubated with 750 μM of Pb. The relationship between Pb and ALAD activity was statistically described by [ALAD Activity] = 0.476−0.000597 × [Pb]. According to the obtained results, we suggest that the ALAD of Burkholderia gladioli can be used as a biomarker for lead contamination in the environment.  相似文献   

4.
The aim of this work was to monitor the sensitivity of two populations of Corophium orientale (Crustacea: Amphipoda) living at the outfall of two rivers (Magra and Serchio), comparing their responses towards two different toxicant solutions. Sensitivity was monthly checked performing the 96h-LC50 static water only test with Cd(NO3)2 and SDS. If no significant differences were found between the two populations, they could have been employed without distinction to perform sediment toxicity bioassays. As regard to Cd, an increasing in LC50 values from summer to winter was evident in each population (Serchio River: August 2003  =  1,36 mg/l, February 2004  =  7,23 mg/l; Magra River: August  =  1,21 mg/l, April  =  5,01 mg/l). This pattern was correlated to the droop of temperatures in winter period. The responses of the two populations towards the cadmium were compared using the ANOVA. The analysis showed any significant differences between the populations (p  =  0.12). The pattern of sensitivity towards SDS for the population living on Magra River was similar to the same pattern found for Cd; as regard to the population living on Serchio River, data were not enough to describe the annual pattern. Anyway, statistical analysis was performed and no significant differences were found between the two populations (p  =  0.34).  相似文献   

5.
Atmospheric surface aerosol radiative forcing (SARF) ΔF, forcing efficiency ΔFe and fractional forcing efficiency ΔFFe evaluated from cloud-screened narrowband spectral and thermal-offset-corrected radiometric observations during the Asia dust outbreak episodes in Gwangju, Republic of Korea are reported in this study. Columnar aerosol optical properties (aerosol optical depth (AOD), τ , Angstrom exponent α, mass concentration of fine and coarse mode particles) were also reported for the station between January 2000 and May 2001 consisting of 211cloud-free days. Results indicate that majority of the AOD were within the range 0.25–0.45 while some high aerosol events in which AODs ≥ 0.6 were observed during the severe dust episodes. For example, AOD increases from annual average value of 0.34 ± 0.13 at 501 nm to values >0.60 during the major dust events of March 27–30 and April 7–9, 2000, respectively. The α 501–870 nm which is often used as a qualitative indicator of aerosol particle size had values ranging from 0.01 to 1.77. The diurnal forcing efficiency ΔDFe at Gwangju was estimated to be −81.10 ± 5.14 W m −2/τ 501 nm and −47.09 ± 2.20 W m −2/τ 501 nm for the total solar broadband and visible band pass, respectively while the fractional diurnal forcing efficiency ΔFDFe were −15.8 ± 0.64%/τ 501 nm and −22.87 ± 1.13%/τ 501 nm for the same band passes. Analyses of the 5-day air-mass back trajectories were further developed for Gwangju in order to classify the air-mass and types of aerosol reaching the site during the Asia dust episodes.  相似文献   

6.
The present study deals with the effect of fireworks on ambient air quality during Diwali Festival in Lucknow City. In this study, PM10, SO2, NO x and 10 trace metals associated with PM10 were estimated at four representative locations, during day and night times for Pre Diwali (day before Diwali) and Diwali day. On Diwali day 24 h average concentration of PM10, SO2, and NO x was found to be 753.3, 139.1, and 107.3 μg m−3, respectively, and these concentrations were found to be higher at 2.49 and 5.67 times for PM10, 1.95 and 6.59 times for SO2 and 1.79 and 2.69 for NO x , when compared with the respective concentration of Pre Diwali and normal day, respectively. On Diwali day, 24 h values for PM10, SO2, and NO x were found to be higher than prescribed limit of National Ambient Air Quality Standard (NAAQS), and exceptionally high (7.53 times) for PM10. On Diwali night (12 h) mean level of PM10, SO2 and NO x was 1,206.2, 205.4 and 149.0 μg m−3, respectively, which was 4.02, 2.82 and 2.27 times higher than their respective daytime concentrations and showed strong correlations (p < 0.01) with each other. The 24 h mean concentration of metals associated with PM10 was found to be in the order of Ca (3,169.44) > Fe (747.23) > Zn (542.62) > Cu (454.03), > Pb (307.54) > Mn (83.90) > Co (78.69) > Cr (42.10) > Ni (41.47) > Cd (34.69) in ng m−3 and all these values were found to be higher than the Pre Diwali (except Fe) and normal day. The metal concentrations on Diwali day were found to be significantly different than normal day (except Fe & Cu). The concentrations of Co, Ni, Cr and Cd on Diwali night were found to be significantly higher than daytime concentrations for Pre Diwali (control). The inter correlation of metals between Ca with Pb, Zn with Ni and Cr, Cu with Co, Co with Mn, Ni with Cd, Mn with Cd, Ni with Cd and Cr, and Cr with Cd showed significant relation either at p < 0.05 or P < 0.01 levels, which indicated that their sources were the same. The metals Cu, Co, Ni, Cr and Cd showed significant (p < 0.01) association with PM10. These results indicate that fireworks during Diwali festival affected the ambient air quality adversely due to emission and accumulation of PM10, SO2, NO x and trace metals. ITRC Communication Number 2538  相似文献   

7.
An experiment was conducted under laboratory conditions to investigate the effect of two systemic herbicides viz., pendimethalin and quizalofop, at their recommended field rates (1.0 kg and 50 g active ingredient ha − 1, respectively) on the growth and activities of non-symbiotic N2-fixing bacteria in relation to mineralization and availability of nitrogen in a Typic Haplustept soil. Both the herbicides, either singly or in a combination, stimulated the growth and activities of N2-fixing bacteria resulting in higher mineralization and availability of nitrogen in soil. The single application of quizalofop increased the proliferation of aerobic non-symbiotic N2-fixing bacteria to the highest extent while that of pendimethalin exerted maximum stimulation to their N2-fixing capacity in soil. Both the herbicides, either alone or in a combination, did not have any significant difference in the stimulation of total nitrogen content and availability of exchangeable NH4  +  while the solubility of NO3  −  was highly manifested when the herbicides were applied separately in soil.  相似文献   

8.
Freshwater cladocera such as Daphnia magna and Ceriodaphnia dubia have been used extensively for freshwater toxicity test worldwide. However, these species may not be indigenous in certain geographical regions, which restrict the utility of these organisms as test species. In the present study, we investigated optimal culture and test conditions for an indigenous freshwater macroinvertebrate of Korea, Moina macrocopa. The culture conditions that were evaluated included water temperature (20°C and 25°C), rearing media (moderately hard water or MHW, with or without selenium supplementation, or Elendt M4), and food density (2.5 × 107 and 5 × 107 cells/mL of Selenastrum capricornutum), and their effects on the life history characteristics of M. macrocopa were determined. Population growth rate of M. macrocopa was maximized at 25°C with 5 × 107 cells/mL of algal food density in MHW. A series of chronic three brood reference toxicant tests were conducted under the ideal culture conditions that were identified here, and the results of the tests indicated reliable reproducibility of the test protocol. Optimal culture and test conditions that were identified for M. macrocopa in the present study are suggested for evaluation of chronic toxicity of chemicals and industrial or municipal discharge.  相似文献   

9.
Flow and Pollutant Dispersion in Street Canyons using FLUENT and ADMS-Urban   总被引:1,自引:0,他引:1  
This paper is devoted to the study of flow within a small building arrangement and pollutant dispersion in street canyons starting from the simplest case of dispersion from a simple traffic source. Flow results from the commercial computational fluid dynamics (CFD) code FLUENT are validated against wind tunnel data (CEDVAL). Dispersion results from FLUENT are analysed using the well-validated atmos pheric dispersion model ADMS-Urban. The k − ε turbulence model and the advection-diffusion (AD) method are used for the CFD simulations. Sensitivity of dispersion results to wind direction within street canyons of aspect ratio equal to 1 is investigated. The analysis shows that the CFD model well reproduces the wind tunnel flow measurements and compares adequately with ADMS-Urban dispersion predictions for a simple traffic source by using a slightly modified k − ε model. It is found that a Schmidt number of 0.4 is the most appropriate number for the simulation of a simple traffic source and in street canyons except for the case when the wind direction is perpendicular to the street canyon axis. For this last case a Schmidt number equal to 0.04 gives the best agreement with ADMS-Urban. Overall the modified k − ε turbulence model may be accurate for the simulation of pollutant dispersion in street canyons provided that an appropriate choice for coefficients in the turbulence model and the Schmidt number in the diffusion model are made.  相似文献   

10.
A pilot study was conducted to evaluate the usefulness of salivary cholinesterase and carboxylesterase as biomarkers of exposure to environmental organophosphate pesticides. Ninety samples were obtained from women and 62 samples from their preschool-aged children who live near an agricultural area of the Upper Valley of the Negro River (Patagonia, Argentina) where pesticides are applied 6 months a year. Each participant donated two samples under similar conditions: one in the pre-exposure period and another during the pulverization period. Demographic information, potential confounders, and risk behaviors were registered. Active or passive smoking had no effect on these enzyme activities in either group. During the pulverization period, cholinesterase activity was not detectable in 76% of the children's samples and 23% of the mothers' samples. Comparing samples collected during the pulverization period with respect to the pre-pulverization period, the average mother and child cholinesterase activity decreased by 65.7% (p < 0.001) and 85.8% (p < 0.001), respectively. Also, mother and child carboxylesterase activity decreased by 27.5% (p < 0.001) and 41.9% (p < 0.01), respectively. Child carboxylesterase activity in the pulverization period was associated to the habit of eating dust outdoors (p < 0.01). The most frequent inhibition levels observed for cholinesterase and carboxylesterase activity were between 70–100% and 0–29%, respectively, in both groups studied. This shows that at the same level of exposure, cholinesterase was more sensitive to inhibition than carboxylesterase. Therefore, carboxylesterase might more properly reflect the degree of environmental organophosphate exposure and may have potential as a novel tool for biomonitoring.  相似文献   

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