反相高效液相色谱测定废水中的酚类

我国标准方法仍采用4-氨基安替比林比色法测定酚类总量。由于显色剂与取代酚不能完全反应,因此总量的测定会引起一定的误差。由于此种方法只能测定酚类的总量,不能选择性地对酚类有害物进行测定,给实际工作带来很大不便。反相高效液相色谱测定酚类化合物可以弥补上述方法的不足。 实验部分 一、主要仪器和设备     

酚类抗氧化剂及其代谢产物对鱼类的毒性研究进展

简述了水环境中合成酚类抗氧化剂(SPAs)及其代谢产物，SPAs对鱼类的急性毒性效应、发育毒性效应及机制、内分泌干扰毒性效应和机制，以及代谢过程和产物毒性预测。指出，为准确实现对SPAs及其代谢物的化学品管理及生态风险评估，未来研究应聚焦于对合成酚类抗氧化剂及其代谢物在水生生物体内的分布及浓度检测，为该类化合物的生物富集和生物转化研究提供更多的背景资料；开展对SPAs潜在毒性分子机制的研究，并建立起分子水平上的毒理学效应与个体乃至种群水平上的不良后果之间的联系；探究并完善SPAs的代谢机制，并对现已明确的代谢产物（如BHT-Q）开展毒性效应评价。     

河流水环境质量综合评价方法比较研究

提出了对河流水环境质量综合评价方法的基本要求，对水环境质量评价方法的现状进行了研究，并通过对各种方法的 比较，探讨了今后水环境质量综合评价方法的发展方向。     

水环境生物监测的发展方向与核心技术

水环境生物监测是环境监测的重要内容,它应重点说清环境胁迫的生物效应。简述了总量管理、流域管理、风险管理、生态管理等环境管理对水环境生物监测有迫切需求,应引入＂生态系统健康＂、＂生物完整性＂、＂环境胁迫＂、＂全排水毒性＂等现代环境生物监测的基本概念,建立水环境生物监测技术发展的理论基础,发展生物完整性、综合毒性等监测与评价核心技术;革新现行监测方法体系,建立包括QA/QC、快速方法等支持系统在内的现代水环境生物监测业务化方法体系;创新评价技术体系,建立水环境生态健康评价及综合毒性评价指标体系、基准及分级管理标准,确立水环境质量管理的生物学目标。     

区域水环境监测网络优化及评价考核方法研究

科学合理地布设水质监测断面是全面准确获取水环境质量监测数据的前提条件。利用研究区域现有断面2014年的监测数据,结合水资源调度方式和水环境功能区划要求,划分为五大片区并采用聚类分析方法进行断面优化,通过F检验和t检验表明优化前后断面无显著差异。同时,提出了一种综合的水环境质量考核办法——区域水环境综合评价考核指标,该指标包括骨干河道、乡村河道两类考核断面,采用综合水质评价与单因子评价相结合的方法,设置适当的权重系数,评价结果较为全面、客观地反映区域水环境质量状况,基本满足对区域水环境质量考核评价的管理需求。     

辽河干流水环境质量监测网络优化研究

通过构建适宜度方法对水环境质量监测网络优化方法进行定量筛选,并以辽河干流为研究区进行水环境监测网络优化方法的筛选、应用和优化结果验证。结果表明,通过适宜度方法的定量筛选,最优分割法更适宜于辽河干流水环境监测网络的优化,优化后辽河干流水环境监测网络由8个断面优化为4个断面。采用T检验和F检验对优化结果验证,优化后的监测网络能很好地代表原监测网络,且相邻断面监测数据相关率降低,监测网络合理性得到提高。     

新疆水环境质量监测数据中存在问题的探讨

根据水环境质量监测数据审核工作实践,总结出目前新疆地、州、市环境监测站上报的水环境质量监测数据中存在的问题,并介绍和分析了几种常用的水质监测数据合理性判断的方法和依据,以期为新疆水环境质量监测工作提供可借鉴的建议。     

水环境中病毒检测技术研究进展

综述了水环境病毒检测技术的研究进展。介绍了过滤法、吸附法、离心法、混凝法等水环境病毒富集方法的原理和优缺点;总结了基于聚合酶链式反应(PCR),包括巢式PCR、多重PCR、实时荧光定量PCR、细胞联合培养PCR和数字PCR,以及高通量测序等分子生物学技术在水环境病毒检测中的应用;指出了水环境中病毒检测技术当前存在的问题和未来发展方向,为水环境中病毒的检测与管控提供技术支撑。     

中国沿海三省主要饮用水源有机物监测

采用固相萃取、吹扫捕集和气相色谱-质谱联用仪(GC-MS)对江苏、浙江、山东省的21个主要地表饮用水源、126份水样中的25种VOCs、38种SVOCs进行定量检测。结果有19种VOCs、10种SVOCs至少在一个水源地多次被检出,其中挥发性氯代烃、苯系物、氯代苯类、酚类、硝基苯类、酞酸酯类、农药为中国或美国环境保护局提出的"水中优先控制污染物",最高检出量为27.79μg/L。三省主要地表水受到有机物轻度污染,水体中VOCs、SVOCs含量尚未超过《地表水环境质量标准》(GB 3838-2002)和《生活饮用水卫生标准》(GB 5749-2006)限值,工业废水排放及农田雨水径流是地表水有机物的重要来源。     

TU-1901紫外可见分光光度计用于污水中酚类和苯胺类的快速测定

介绍了用ＴＵ１９０１紫外可见分光光度计直接、快速测定环境污水中酚类和苯胺类的实验方法。污水样品用１∶１盐酸调ｐＨ为３０±０１,直接测绘吸收光谱,微机４阶微分后即可同时获得酚类和苯胺类的含量,方法简捷、快速、节省试剂且无污染,所用仪器为国产的先进仪器,在环境监测领域有广阔的应用前景     

衍生化吹扫捕集-气相色谱-质谱法测定饮用水中的6种氯酚

建立了饮用水中6种氯酚的检测方法。调节水样pH后,经乙酸酐衍生化、吹扫捕集后经气相色谱-质谱测定,外标法定量。在0.004～0.120mg/L范围内,方法的线性关系良好,相关系数为0.9972～0.9999,加标回收率为94.2%～112%,相对标准偏差为1.88%～5.27%。该方法简便、灵敏度高,完全可以满足饮用水中6种氯酚的痕量检测要求。     

Development of an enzyme-linked immunosorbent assay for screening contamination by chlorophenols in environmental waters

The development of an immunoassay for screening contamination by chlorophenols is presented. Two haptens were synthesized and conjugated to immunizing proteins to raise rabbit polyclonal antibodies. The antibody-coated format (direct) gave better sensitivity than the conjugate-coated format (indirect) if 2,4,6-trichlorophenol is used as target analyte. The measurement range was 86.4 microg l(-1) to 0.7 microg l(-1), with an average I50 of 7.8 microg l(-1) and a detection limit of 0.2 microg l(-1). The assay detects the presence of trichloropyridinol and other chlorophenols such as di-, tetra- and pentachlorophenols constituting thus a suitable tool for the early warning of the presence of such family contaminants. The optimized method permits the detection of the most important chlorophenols in a fast and reproducible way using no more than one antibody and a single assay. The results achieved with water samples spiked with different chlorophenols fit with a multiple linear regression model when expressing the total concentration of chlorophenols as equivalent of 2,4,6-trichlorophenol (P < 0.01), demonstrating the usefulness of the assay as a screening tool to detect contamination by chlorophenols.     

Magnetic microsphere confined ionic liquid as a novel sorbent for the determination of chlorophenols in environmental water samples by liquid chromatography

Magnetic microsphere confined ionic liquid was synthesized by covalently bonding N-methylimidazolium on magnetic microspheres. The functionalized magnetic microspheres were characterized by Fourier transform infrared (FT-IR) spectrometry, thermogravimetric analysis (TGA) and scanning electron microscopy (SEM). The prepared material was used for the preconcentration of three chlorophenols (CPs) in water combined with high-performance liquid chromatography (HPLC). Several conditions that probably affected the extraction efficiency such as standing time, eluent and its volume, sample pH and volume, were optimized. Under the optimal conditions, good recoveries (70.3-88.8%) were achieved with satisfactory relative standard deviations (RSDs) of less than 6.0%. The limits of detection for the three CPs were 0.20-0.35 μg L(-1). The results indicated that the ionic liquid-functionalized magnetic microspheres show significant promise for the analysis of CPs in environmental samples.     

Performance optimization of a membrane assisted passive sampler for monitoring of ionizable organic compounds in water

A thin-walled silicone rubber hollow fibre membrane has been developed as a passive sampler. The inside of the tube is filled with an aqueous solution at an appropriate pH. The tube is sealed at both ends and then immersed in a water sample. In order for the ionizable permeating compounds to be trapped in the aqueous receiving phase, the pH is adjusted such that the compounds are ionized and trapped. The major advantages are its simplicity, low cost and high selectivity, since only ionizable organic compounds are trapped. Additionally, the sampler uses no organic solvent. By adjusting the pH of the acceptor phase, it is possible to control the extraction process and whether the sampler is used in the kinetic or equilibrium regime. Since it is very selective, no further clean-up of the extract is required. The membrane assisted passive sampler has been tested for extraction of chlorophenols under laboratory conditions. The extraction process was found to be linear over a 72 h sampling period. Selectivity of the passive sampler in river water was demonstrated and the extraction process was independent of sample concentration, even at lower concentration levels of analytes. However, the sample matrix in some river water samples led to incomplete trapping, thereby reducing the amount trapped in the acceptor phase. Detection limits (three times signal to noise ratio) were dependant on sample matrix and type of detection system and ranged from 0.05 microg L(-1) to about 1 microg L(-1) with a UV photodiode detector in water samples from one river and 1.0 microg L(-1) to 20 microg L(-1) in another but with an ordinary UV detector. The enrichment factors in river water were 28 for 2-chlorophenol and 44 for 2,4-dichlorophenol over a 72 h sampling period. 4-chlorophenol was poorly extracted and its enrichment factor was 3.     

Biotreatability and kinetics of UASB reactor to mixtures of chlorophenol pollutants

In most natural ecosystems heterotrophic microorganisms encountercomplex mixtures of carbon sources, each of which is present at aconcentration of few micrograms per litre. This study examined the biotreatability and kinetics of an upflow anaerobic sludge blanket (UASB) reactor to complex mixtures of chlorophenols encountered in environmental conditions using on-line and off-line experimental studies. Results indicate that (1) steady-state concentration was quite lower (98.3 mg L^-1) with complex mixture of chlorophenols than steady-state concentration achieved when only 2.4 dichlorophenol (124 mg L^-1) was studied alone on the same reactor; (2) that toxiceffects of chlorophenols increase with increasing concentrationsof toxicant. (Onset of the inhibitory effect occurred at a lowerconcentration in multi-substrate than in single substrate utilization); (3) addition of alternative utilizable substrate can mitigate toxic effects and enhance degradation; (4) the relative concentration of substrate was critical in determiningutilization patterns. HPLC analysis of off-line experimental samples resulted in a steady-state treatment efficiency of68% for COD, 36% for 2-hlorophenol, 40.5% for 4-chlorophenol, 70.7% for 2,4-dichlorophenol, 53.2% for 2,4,6-trichlorophenol and 42% for pentachlorophenol in presence of glucose. Kinetic constant in terms of V _max and K _s were determined. K _s for the five chlorophenols ranged between 0.016 and 0.117 kg m^-3 day^-1 while V _max ranged between 0.056 and 0.244 kg m^-3 day^-1.     

Investigation of the use of honey bees and honey bee products to assess heavy metals contamination

Forty-four water samples from eleven sampling points were collected from four highly polluted rivers of northern India once in each four seasons during 1988–1989. The samples were analyzed for phenol, chlorophenols, a few bromophenols and other organics. Phenol was found to be absent in all the analyzed samples. Trichlorophenol and pentachlorophenol were frequently detected. Comparatively, the Ganges river was most polluted at Kannauj followed by Narora, Kachala and Fatehgarh. Maximum phenols were found at Mathura downstream of the Yamuna river followed by Mathura upstream, Okhla, ITO and none at Wazirabad. No phenols were detected in the water of the rivers Hindon and Kali at Ghaziabad and Aligarh, respectively. Some other organic pollutants were also identified by their mass spectra and supported by data from the computerized library, but, not quantified.To whom correspondence should be addressed.     

Development of a robust chromatographic method for the detection of chlorophenols in cork oak forest soils

A major concern for the cork and wine industry is ‘cork taint’ which is associated with chloroanisoles, the microbial degradation metabolites of chlorophenols. The use of chlorophenolic compounds as pesticides within cork forests was prohibited in 1993 in the European Union (EU) following the introduction of industry guidance. However, cork produced outside the EU is still thought to be affected and simple, robust methods for chlorophenol analysis are required for wider environmental assessment by industry and local environmental regulators. Soil samples were collected from three common-use forests in Tunisia and from one privately owned forest in Sardinia, providing examples of varied management practice and degree of human intervention. These provided challenge samples for the optimisation of a HPLC-UV detection method. It produced recoveries consistently >75 % against a soil CRM (ERM-CC008) for pentachlorophenol. The optimised method, with ultraviolet (diode array) detection is able to separate and quantify 16 different chlorophenols at field concentrations greater than the limits of detection ranging from 6.5 to 191.3 μg/kg (dry weight). Application to a range of field samples demonstrated the absence of widespread contamination in forest soils at sites sampled in Sardinia and Tunisia.     

固相萃取-高效液相色谱法测定饮用水中酚类化合物

采用固相萃取-高效液相色谱法测定饮用水中酚类化合物,优化了试验条件。方法在0 mg/L~40.0 mg/L范围内线性良好,14种酚类化合物的检出限为0.6μg/L~2.6μg/L,水样平行测定的RSD为0.2%~2.7%,加标回收率为96.3%~99.9%。     

Water quality change in dam reservoir and shallow aquifer: analysis on trend,seasonal variability and data reduction

Change of water quality in dam reservoir and aquifer complicates safe drinking water supply. Few parameters are monitored to control water quality in these sources. Adequate knowledge on the correlation structure, interaction effect, trends and seasonal variability of these parameters is essential to control water quality. This study applied time series and multivariate analyses on 15 water quality parameters, collected from the King Fahd dam reservoir (L1) and aquifer (L2) in Saudi Arabia during April 2010 to February 2012. Moderate to strong correlations were observed between sulfate, hardness, fluoride, chloride, magnesium, conductivity, turbidity and total dissolved solids (TDS), while separate clusters were visible for TDS-chloride-magnesium-conductivity; fluoride-turbidity; chloride-hardness; ammonia-nitrate; and calcium-magnesium-hardness. Four major principal components explained 81.1 % and 83.2 % of the overall variances in L1 and L2, respectively. The factor analysis showed that 53 % and 67 % of the data were necessary to explain 81.3 % and 83.2 % of total variances for L1 and L2, respectively, indicating the possibility of data reduction. Possible degradation of water quality in these sources was highlighted, while such degradation may require enhanced treatment for producing drinking water in future.     

电导和直流安培双检测器离子色谱法测定清洁水样中的碘化物

选用配备了2种不同检测器(电导检测器和直流安培检测器)的离子色谱仪对稀释后过0.22μm滤膜的水样进行分析。配备有直流安培检测器的离子色谱仪测定水中碘化物的方法在0.100~20.0μg/L范围内线性关系良好,相关系数(r)=0.9999,方法检出限为0.030μg/L,测定下限为0.120μg/L,样品加标回收率为95.0%~104%,相对标准偏差为1.06%~1.64%;配备有电导检测器的离子色谱仪测定水中碘化物的方法在20.0~2.00×105μg/L范围内线性关系良好,相关系数(r)=0.9995,方法检出限为2.00μg/L,测定下限为8.00μg/L,样品加标回收率为99.0%~110%,相对标准偏差为0.71%~3.12%。离子色谱-直流安培检测器法测定水中碘化物的方法准确度高、灵敏度高、精密度好,检出限相对较低,适用于测定ρ(碘化物)≤20.0μg/L的清洁水样;离子色谱-电导检测器法主要适用于测定ρ(碘化物)≥20.0μg/L的水样。