共查询到19条相似文献,搜索用时 78 毫秒
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为了解南京玄武湖蓝藻水华的成因,对玄武湖长期监测资料进行了分析和现场调查。结果表明,玄武湖鲢鳙急剧减少中断了正常的食物链,使浮游植物的牧食压力降低,在东南湖回水的诱因作用下,发生了以微囊藻为优势种群的蓝藻水华。指出,玄武湖蓝藻水华的发生进程表明,东南湖回水可能是蓝藻水华发生的诱因,生态失衡是蓝藻水华发生的主要原因,蓝藻水华的暴发与富营养化程度无显著相关性,其暴发加重了玄武湖富营养化程度。 相似文献
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微囊藻毒素检测方法的研究进展 总被引:1,自引:1,他引:1
随着水体富营养化日益严重,有毒蓝藻水华引起的污染事件在世界范围内频繁发生,其产生的毒素对生物有潜在的危害。所以迫切需要建立一种工作原理简单易行、分析速度快、灵敏度较高的统一的检测方法,对水体中特别是饮用水源中的微囊藻毒素进行检测。文章简要介绍了近年来发展起来的几类微囊藻毒素检测技术,以及微囊藻毒素检测技术尚要解决的问题和未来的发展方向。 相似文献
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以铜绿微囊藻(Microcystis aeruginosa)16S rRNA基因片段为靶序列设计一对特异性引物,采用Real-time PCR法,对铜绿微囊藻进行定性、定量检测。试验表明,仅含铜绿微囊藻DNA模板的样品有特异性扩增,扩增产物熔解曲线平稳,峰尖且窄,熔解温度为(87±1)℃。以重组质粒pMD-18T-16S为标准品,检测区间为1.1×102 copies/mL~1.1×108 copies/mL,所得标准曲线符合制备实时定量PCR标准曲线的要求,对标准品进行测定,方法检出限为11 copies/mL。用该标准曲线对实验室培养获得的铜绿微囊藻DNA样品进行定量检测,与显微镜计数结果基本一致。 相似文献
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太湖蓝藻水华预警监测综合系统的构建 总被引:2,自引:2,他引:0
近年来随着浅水型湖泊的富营养化进程不断加快,蓝藻水华暴发现象也频繁出现,采用科学、全面的手段对太湖蓝藻暴发进行预警十分必要。根据太湖蓝藻预警监测中使用的现场巡视、卫星遥感、实验室分析、自动监测等监测技术手段,分别建立各自监测系统,结合各监测系统特点和相互关系,对太湖蓝藻水华预警监测综合系统的构建进行了探讨,以期能够更好地开展太湖蓝藻水华预警监测工作,为确保太湖地区饮用水安全,提高环保部门应对太湖蓝藻水华暴发的能力,为政府决策提供技术支持和保障。 相似文献
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实时荧光定量聚合酶链反应快速检测产毒铜绿微囊藻 总被引:1,自引:0,他引:1
以铜绿微囊藻(Microcystis aeruginosa)产毒株微囊藻毒素合成酶基因(mcyA)为靶序列设计了一对特异性引物,运用实时荧光定量聚合酶链反应(qPCR)方法,对铜绿微囊藻进行了定性定量检测。结果表明,仅含铜绿微囊藻脱氧核糖核酸(DNA)模板的样品有扩增,对照组小球藻(Chlorella)没有检测到扩增信号;扩增产物熔解曲线平稳,峰尖且窄,熔解温度为(83±1)℃,证明该聚合酶链反应扩增产物极为特异。以重组质粒pMD-18T-mcyA为标准品,所得标准曲线具有高度线性相关性,重复性好,符合制备实时定量聚合酶链反应标准曲线的要求。利用该方法建立的标准曲线对实验室培养获得的铜绿微囊藻DNA样品进行定量检测,与显微镜计数结果基本一致。 相似文献
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Detection of microcystins in Pamvotis lake water and assessment of cyanobacterial bloom toxicity 总被引:1,自引:0,他引:1
Papadimitriou T Armeni E Stalikas CD Kagalou I Leonardos ID 《Environmental monitoring and assessment》2012,184(5):3043-3052
Lake Pamvotis is a shallow, eutrophic Mediterranean lake with ecological significance. This paper deals with the evaluation
of cyanobacterial toxicity in Lake Pamvotis. ELISA and HPLC revealed the presence of significant amounts of MCYST-LR. Danio rerio bioassay confirmed the toxic nature of the bloom. Cyanobacterial extracts had adverse toxic effects on development of D. rerio. Also, it was shown that cyanobacterial extracts containing environmentally detected concentrations of MCYST can cause reduced
survival rate of fish species. The results clearly indicate that cyanobacterial blooms in Lake Pamvotis may be regarded as
human and fish health hazard. Continuous monitoring of the lake is suggested, in order to prevent future possible intoxications. 相似文献
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The dynamics of toxic and nontoxic Microcystis during bloom in the large shallow lake,Lake Taihu,China 总被引:1,自引:0,他引:1
Daming Li Yang Yu Zhen Yang Fanxiang Kong Tongqing Zhang Shengkai Tang 《Environmental monitoring and assessment》2014,186(5):3053-3062
Lake Taihu is a large shallow freshwater lake (surface area 2,338 km2, mean depth 1.9 m) in China, which has experienced toxic cyanobacterial bloom dominated by Microcystis annually during the last few decades. In the present study, the dynamics of toxic and nontoxic Microcystis in three sampling stations (Meiliang Bay (site N2), Gonghu Bay (site N4), and the lake center area (site S4)) were quantified using quantitative real-time PCR (qPCR) during bloom periods from April to September, 2010. Our data showed that the abundance of toxic Microcystis and the toxic proportion gradually increased from April to August in water samples and reached the peak in August. During the study period, toxic Microcystis genotypes comprised between 26.2 and 64.3, between 4.4 and 22.1, and between 10.4 and 20.6 % of the total Microcystis populations in the three sampling sites, respectively. Correlation analysis suggested that there was a strong positive relationship between total Microcystis, toxic Microcystis and the toxic proportion. Chlorophyll a, total phosphorus, and water temperature were positively correlated with the abundances of total Microcystis and toxic Microcystis. Furthermore, the toxic proportion was positively correlated with total phosphorus (P?<?0.05) and water temperature (P?<?0.01), showing that global warming together with eutrophication could promote more frequent toxic blooms. 相似文献
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通过对3S技术整合,提出"MMA"技术路线应用于蓝藻水华监测,包括监测、测绘、分析等关键步骤。对太湖蓝藻水华研究结果表明,总体规律一致,但又有所差异。一方面,太湖蓝藻密度(CBD)、叶绿素a(Chl-a)及水华频率分布图结果均呈现"西高东低"的空间分布规律;另一方面,太湖湖心区CBD和Chl-a浓度亦较高,而遥感监测后的水华频率图显示为无水华或频率小于1%。故应参照"MMA"技术路线,综合应用3S技术,并核验比对,弥补单项技术存在的不足,全面真实反映藻类水华情况。"MMA"技术路线既适用于水华监测,亦可推广至其他环境监测工作。 相似文献
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巢湖富营养化遥感监测 总被引:1,自引:0,他引:1
以巢湖为研究区,通过对蓝藻水华暴发程度与其光谱反射率之间关系的研究,确定MOD IS遥感影像识别水华暴发级别的阈值,对4类不同暴发程度蓝藻水华光谱特征进行遥感识别;进而确立巢湖水体富营养化评价方法,建立巢湖富营养化遥感反演模型,为实时监控巢湖水质,预警蓝藻水华暴发提供技术支持。 相似文献
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Zamyadi A McQuaid N Prévost M Dorner S 《Journal of environmental monitoring : JEM》2012,14(2):579-588
Toxic cyanobacteria threaten the water quality of drinking water sources across the globe. Two such water bodies in Canada (a reservoir on the Yamaska River and a bay of Lake Champlain in Québec) were monitored using a YSI 6600 V2-4 (YSI, Yellow Springs, Ohio, USA) submersible multi-probe measuring in vivo phycocyanin (PC) and chlorophyll-a (Chl-a) fluorescence, pH, dissolved oxygen, conductivity, temperature, and turbidity in parallel. The linearity of the in vivo fluorescence PC and Chl-a probe measurements were validated in the laboratory with Microcystis aeruginosa (r(2) = 0.96 and r(2) = 0.82 respectively). Under environmental conditions, in vivo PC fluorescence was strongly correlated with extracted PC (r = 0.79) while in vivo Chl-a fluorescence had a weaker relationship with extracted Chl-a (r = 0.23). Multiple regression analysis revealed significant correlations between extracted Chl-a, extracted PC and cyanobacterial biovolume and in vivo fluorescence parameters measured by the sensors (i.e. turbidity and pH). This information will help water authorities select the in vivo parameters that are the most useful indicators for monitoring cyanobacteria. Despite highly toxic cyanobacterial bloom development 10 m from the drinking water treatment plant's (DWTP) intake on several sampling dates, low in vivo PC fluorescence, cyanobacterial biovolume, and microcystin concentrations were detected in the plant's untreated water. The reservoir's hydrodynamics appear to have prevented the transport of toxins and cells into the DWTP which would have deteriorated the water quality. The multi-probe readings and toxin analyses provided critical evidence that the DWTP's untreated water was unaffected by the toxic cyanobacterial blooms present in its source water. 相似文献
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典型湖泊水华特征及相关影响因素分析 总被引:1,自引:0,他引:1
通过2011-2015年对太湖、巢湖和滇池水华高发季节的连续监测,以藻类密度和水华面积为判据评价了3个湖体的水华情况及变化趋势,探讨了水华发生的主要影响因素。结果表明:太湖水华程度以"轻度水华"为主,巢湖水华程度以"轻微水华"为主,滇池水华程度以"中度水华"为主;太湖、巢湖和滇池水华规模均以"零星性水华"为主;太湖和巢湖藻类密度与水温、pH、溶解氧、总氮、总磷和高锰酸盐指数均呈显著正相关,与透明度呈显著负相关,与氨氮无显著相关性;滇池藻类密度与水温、总磷和高锰酸盐指数均呈显著正相关,与透明度和氨氮呈显著负相关,与pH、溶解氧和总氮无显著相关性。 相似文献