生物传感器在环境监测中的应用分析

简要论述了生物传感器的基本原理及特点,重点剖析了生物传感器在水、大气及其他环境监测中应用,展望了生物传感器未来的发展前景。     

生物传感器在环境监测中的应用分析

简要论述了生物传感器的基本原理及特点,重点剖析了生物传感器在水、大气及其他环境监测中应用,展望了生物传感器未来的发展前景。     

生物传感器在环境监测中的应用

生物传感器作为一类新兴传感器,它是以生物分子敏感元件,将化学信号、热信号、光信号转换成电信号或者直接产生电信号予以放大输出,从而得到检测结果。文章综述了生物传感器在环境监测,包括水环境、大气环境等领域的应用和最新进展,并展望了环境监测生物传感器的发展前景及发展方向。     

用于化学与生物检测的光子传感器研究进展

文章回顾了近年来光子集成化学与生物传感器的一些新进展,主要讨论表面等离子体波生物传感器,基于环形谐振器的化学和生物光学传感器和集成光学干涉生物传感器(主要涉及2种:集成双模波导干涉生物传感器和基于马赫-曾德尔干涉仪(MZI)的生物光子传感器),并特别注意对材料,技术架构,光学传感原则和各自优缺点的简要阐述。此外,还指出了实现上述传感器设计与仿真的软件。     

BOD生物传感器的研究与发展

综述了BOD生物传感器的应用与最新进展。主要介绍了BOD生物传感器的基本原理、特点、研究现状及发展趋势。     

BOD生物传感器的研究

报道了BoD生物传感器的研制过程,考查了温度、pH对生物电极的影响,并确定了最佳工作条件,以及BOD生物传感器的实际应用情况。     

浅议生物传感器在环境监测中的应用

生物传感器是一项综合了多门学科的高新技术,具有特异性好、灵敏度高、分析速度快、能在复杂体系中在线连续监测等特点,被广泛用于多个领域。本文详细介绍了生物传感器相关知识,讨论了生物传感器在环境监测领域中的应用。     

生物传感器在环境污染物检测中的应用

本文介绍了生物传感器的基本组成、工作原理、分类，并重点论述了生物传感器在农药、废水、酸雨等环境污染物检测中的应用。     

生物传感器在渔业监测中的研究进展

论述了生物传感器在监测渔业环境中的BOD、N、P、病原体、农药和重金属等方面的国内外最新进展 ,分析了生物传感器在海洋渔业应用中存在的主要问题及原因 ,同时阐述了利用基因工程微生物技术、分子烙印技术、多成分实时分析技术等先进技术提高生物传感器的性能并扩大其在海洋渔业领域中的应用范围 ,在海洋渔业环境中为实现生物传感器与计算机在线自动监测与控制 ,开拓新的广阔前景。     

一种新的环境监测手段——生物电化学传感器的原理及应用

近年来,随着膜技术的进步,生物传感器的研究和应用得到很大的发展。其中应用得最为广泛的生物传感器就是生物电化学传感器。和其他类型的生物传感器一样,生物电化学传感器也是以生物膜本身所具有的精巧技能作为识别手段的。根据生物膜组成的不同,从分子集合体水平(如固相酶膜)、细胞内生物小器官水平(如固相细胞器膜)、细胞(如固相微生物膜)水平到生物组织(如生物组织切片膜)水平组成不同型式的生物电化学传感器。本文分别作简要介绍。     

Polymer materials in biosensors

Fundamentals and application examples of polymeric materials in different types of biosensors are presented and discussed in view of their molecular structure and biosensor design and construction. The role of a series of polymers with respect to their typical application and their specific properties, like sensitivity and stability, is highlighted. Future trends of polymer materials for biosensors in the area of medical and environmental applications are outlined.     

Long-term stabilization of enzyme activity by immobilizing enzyme colony in polymaleimidostyrene-modified micelles

Amperometric biosensors fabricated by immobilizing enzyme colony in polymaleimidostyrene-modified micelles exhibited good sensitivity and great long-term stability. To our knowledge, there has not been a general design for various enzyme biosensors. Enzyme micelle membrane which is different from any of other conventional immobilization methods, is an innovative way and will be a well-developed biosensor technology to provide rapid and reliable measurements. The potential of using enzyme micelle membrane to fabricate biosensors will be of great hope.     

BOD快速测定传感器的微生物膜固定方法研究

分别采用海藻酸钠包埋法、醋酸纤维素夹层法、戊二醛－牛血清白蛋白交联法等几种物理、化学方法制备固定化微生物膜,并采用自制的微生物ＢＯＤ传感器试验了各种膜对电极响应灵敏度,线性范围,响应时间,稳定性和寿命等性能的影响,比较了不同ｐＨ,温度和交联剂,包埋剂用量下该传感器的性能,获得了较满意的结果,实验表明,以交联法制备的传感器性能最佳,电极寿命达４０ｄ,测定ＢＯＤ值时间为１０－１５ｍｉｎ,方法的重视性和     

Applications of biosensors for bacteria and virus detection in food and water–A systematic review

Biosensors for sensitive and specific detection of foodborne and waterborne pathogens are particularly valued for their portability, usability, relatively low cost, and real-time or near real-time response. Their application is widespread in several domains, including environmental monitoring. The main limitation of currently developed biosensors is a lack of sensitivity and specificity in complex matrices. Due to increased interest in biosensor development, we conducted a systematic review, com...     

Biosensoren

By the combination of transducers (thermistors, selective electrodes, field-effect transistors, optical systems) with immobilized enzymes or antibodies specific sensors for biologically relevant substances are obtained. The construction, ranges, of linearity, response times and stability of biosensors are demonstrated. Examples are given for their application in clinical analysis and fermentation control. Finally limits and future possibilities are discussed.     

Biosensors: new approaches in drug discovery

The development of biosensors for analytical purposes has attracted a great deal of attention in recent years. A biosensor is defined as an analytical device consisting of a biological component (e.g., enzyme, antibody, entire cell, DNA) and a physical transducer (e.g., electrode, optical device). Biosensors are mostly designed for routine analysis, such as clinical diagnosis, quality control of food, in-process control of fermentations, and in environmental analysis. Many of these sensors are also suitable for screening purposes in order to find new drugs. Such systems should yield information either about compounds with known bioactivity or about the bioactivity of samples with known or unknown chemical composition. Biosensors intended for the latter purpose are essentially based on whole cells carrying receptors and ion channels at their surfaces. Miniaturization of structures, primarily based on silicon, allows integration of many sensors into arrays, which may be suitable for the screening of natural and chemical products as well as combinatorial libraries. Until now, no commercially available sensors of this kind exist but they are expected in the near future. Different biosensors, based on enzymes, antibodies, cells, artificial membranes and entire animal tissues, which can be used in drug discovery and may lead to efficient screening systems in the future, are described in this review.     

Validation of a conductometric bienzyme biosensor for the detection of proteins as marker of organic matter in river samples

This article describes a conductometric bi-layer based bienzyme biosensor for the detection of proteins as a marker of organic matter in rivers. Proteins were chosen to be used as indicators of urban pollution. The working mechanism of the bienzyme biosensor is based on the enzymatic hydrolysis of proteins into several fractions (peptides and amino acids), which results in a local conductivity change depending of the concentration of proteins. In this work, we began with the optimization of biosensor response using bovine serum albumin (BSA) as standard protein. For this objective seven enzymatic biosensors were prepared: four enzymatic sensors with only one layer of enzyme (proteinase K, trypsin, pronase or protease X) and three other enzymatic sensors with two layers (first layer: membrane containing proteinase K; second layer: one of the three other enzymes: trypsin, pronase or protease X). The biosensors were obtained through the deposition of enzymatic layers and the cross-linking process between enzymes and BSA in saturated glutaraldehyde vapour. The response of the various biosensors, described previously, were compared with the values of total organic carbon (TOC), and those of organic nitrogen (Norg), as determined by the laboratory accredits (CEMAGREF of Lyon) using the traditional method of analysis (NF EN 1484, infrared spectroscopy) and (NF EN 25663, mineralization/colorimetry assay) respectively for each water sample obtained from di erent sites in Lyon (France). The linear correlations obtained with the response of the seven biosensors showed the most important indices of correlations for the biosensor with two enzymatic layers: proteinase K + pronase (pkp). The optimum conditions for the preparation of the pkp biosensor increased the sensitivity and gave a limit of quantification of 0.583 g/L for TOC and 0.218 g/L for Norg in water samples. This sensor shows good reproducibility (2.28%), a capacity to be used at temperatures range 10– 30°C (depending on the season) and moreover a long lifetime (5 weeks).     

传感器及其在水质监测中的应用

介绍了化学传感器，光导纤维传感器及生物传感器的工作原理，论述了它们在水质监测中的应用，对传感器再生性问题，小型化问题，载体材料问题进行了探讨。     

Trends and Perspectives of Biosensors for Food and Environmental Virology

Food and environmental virology has become a very important and interesting area of research because of food safety and public health concerns. During the last few decades, increasing foodborne diseases and environmental generated illnesses are considered to be highly challenging issues. Biosensor technology holds great promise for the healthcare market, and the security sector. Similar to clinical diagnostic tools, biosensors are being developed for the rapid, reliable, yet inexpensive identification and enumeration of pathogenic viruses which are adulterating environment, food and feed commodities. In this modern era, bio-and nano-technologies play a pivotal role in virological diagnostics of food industry, environmental and veterinary samples. This review covers the recent advances and future prospects of nanotechnology-based bioanalytical microsystems for food and environmental virology.