共查询到17条相似文献,搜索用时 96 毫秒
1.
基于氧猝灭的有机改性溶胶-凝胶微生物传感器测定BOD的研究 总被引:6,自引:1,他引:6
通过有机改性溶胶凝胶PVA包埋方法固定微生物菌株,以氧光化学传感器作为二次传感,制备了响应良好的光化学BOD微生物传感器.在此基础上,考察了微生物传感膜的活化浓度、温度、pH、无机盐及部分金属离子对传感器响应的影响.并对传感器的重现性、储存稳定性和线性范围进行了考察,实验结果表明BOD含量在0—100mg/L浓度范围内与传感器的荧光值成线性关系,传感膜的响应活性保持较好,具有较长的使用寿命和良好的稳定性. 相似文献
2.
本文探讨了用微生物传感器法测定水中的BOD.进行了BOD标准样品的分析和五日生化需氧量法、微生物传感器法的对比实验.实验表明该方法能满足环境监测的要求.为BOD的测定提供了简便、可行的方法. 相似文献
3.
4.
本文探讨了用微生物传感器快速测定法对水中BOD的测定.实验表明,该方法的各项技术指标均达到国家规范要求,为BOD的测定提供了一种简便、可行的新方法. 相似文献
5.
用微生物传感器快速法和稀释接种法对地表水和污水处理厂出水中的生化需氧量(BOD)进行了测定,用数理统计的方法对两种方法测定的结果进行了比较。 相似文献
6.
《环境与可持续发展》2017,(3)
本文采用220B型BOD快速测定仪对标准样品及水样实际样品进行BOD快速测定,并与五日生化稀释接种法进行了比对分析。在大量样品分析的基础上,阐述了微生物传感器快速测定法测定BOD与五日生化稀释接种法相比存在的优缺点。 相似文献
7.
微生物膜BOD电极的研究 总被引:4,自引:1,他引:4
本文介绍了用四种微生物膜制备的BOD电极及其测定废水BOD的试验情况,结果表明,电极对BOD际准物质线性响应范围为10—60mg/L,响应达到平衡时间为4 min(对低限浓度溶液)到7 min(对高限浓度溶液),连续稳定地工怍寿命在20天以上;用该电极和BOD_s标准法对照测定7种废水的BOD值,测定结果的相关性较好。 相似文献
8.
1前言BOD是水质监测的重要项目之一。它用于评价水质受污染程度,是水质有机污染综合性指标;它用于评价生化处理构筑物的效率,探明废水的可生化降能性,并为设计污水处理厂工艺设备提供参数。BOD测定是一种经验的生物实验方法,BOD测定是在某种指定条件下,由生物化学和化学作用共同产生的结果。为得到可靠BOD值就必须充分理解测定内容,严格按操作规范进行。因为当变更任何一种条件时,都将影响BOD值。这些条件包括PH值、温度、微生物的种类和数量、稀释度,以及妨碍微生物增殖的物质和过剩溶解氧等等。另外,由于BOD实验周期较… 相似文献
9.
10.
《石油化工环境保护》1987,(1)
BOD(生化需氧量)是环境监测及污水处理中评价水质的一项重要指标。然而,BOD 的测定一直沿用传统的稀释法(BOD_5),操作繁杂、重现性差、耗时太长(5天),不能及时指导水质评价和有效控制污水处理。迄今,国际上已推荐的几种BOD 快速测定法中,以微生物传感器法具有真正快速、简便、重复性好等特点,使 BOD 相似文献
11.
12.
生物传感器BOD快速测定仪的研究进展 总被引:1,自引:0,他引:1
生化需氧量(biochemical oxygen demand,BOD)作为表征水体中种类繁多的有机污染物的集合参数,在水质评价、污染监测以及废水处理厂的运行分析等方面发挥着重要作用.然而,传统的BOD测量需要5d,费时费力,不能及时反馈相关消息.因此,研究与开发方便、快速、准确的BOD测定仪受到了国内外学者的广泛关注.此文评述了BOD生物传感器快速测定仅的组成、性能、测定条件、响应速度等方面的国内外研究现状,分析了目前BOD传感器研究与开发中存在的问题和局限,并提出了今后进一步研究的方向. 相似文献
13.
KHADRO Basm NAMOUR Philippe BESSUEILLE Franc?ois LEONARD Didier JAFFREZIC-RENAULT Nicole 《环境科学学报(英文版)》2009,21(4):545-551
This article describes a conductometric bi-layer based bienzyme biosensor for the detection of proteins as a marker of organic matter
in rivers. Proteins were chosen to be used as indicators of urban pollution. The working mechanism of the bienzyme biosensor is based
on the enzymatic hydrolysis of proteins into several fractions (peptides and amino acids), which results in a local conductivity change
depending of the concentration of proteins. In this work, we began with the optimization of biosensor response using bovine serum
albumin (BSA) as standard protein. For this objective seven enzymatic biosensors were prepared: four enzymatic sensors with only one
layer of enzyme (proteinase K, trypsin, pronase or protease X) and three other enzymatic sensors with two layers (first layer: membrane
containing proteinase K; second layer: one of the three other enzymes: trypsin, pronase or protease X). The biosensors were obtained
through the deposition of enzymatic layers and the cross-linking process between enzymes and BSA in saturated glutaraldehyde vapour.
The response of the various biosensors, described previously, were compared with the values of total organic carbon (TOC), and those of
organic nitrogen (Norg), as determined by the laboratory accredits (CEMAGREF of Lyon) using the traditional method of analysis (NF
EN 1484, infrared spectroscopy) and (NF EN 25663, mineralization/colorimetry assay) respectively for each water sample obtained
from di erent sites in Lyon (France). The linear correlations obtained with the response of the seven biosensors showed the most
important indices of correlations for the biosensor with two enzymatic layers: proteinase K + pronase (pkp). The optimum conditions
for the preparation of the pkp biosensor increased the sensitivity and gave a limit of quantification of 0.583 g/L for TOC and 0.218
g/L for Norg in water samples. This sensor shows good reproducibility (2.28%), a capacity to be used at temperatures range 10–
30°C (depending on the season) and moreover a long lifetime (5 weeks). 相似文献
14.
15.
16.
A GFP-based bacterial biosensor with chromosomally integrated sensing cassette for quantitative detection of Hg(II) in environment 总被引:3,自引:0,他引:3
Himanshu Priyadarshi Absar Alam Gireesh-Babu P Rekha Das Pankaj Kishore Shivendra Kumar Aparna Chaudhari 《环境科学学报(英文版)》2012,24(5):963-968
A mercury biosensor was constructed by integrating biosensor genetic elements into E. coli JM109 chromosome in a single copy number, using the attP/attB recombination mechanism of λphage. The genetic elements used include a regulatory protein gene (merR) along with operator/promoter (O/P) derived from the mercury resistance operon from pDU1358 plasmid of Serratia marcescens. The expression of reporter gene gfp is also controlled by merR/O/P. Integration of the construct into the chromosome was done to increase the stability and precision of the biosensor. This biosensor could detect Hg(II) ions in the concentration range of 100-1700 nmol/L, and manifest the result as the expression of GFP. The GFP expression was significantly different (P ≤ 0.05) for each concentration of inducing Hg(II) ions in the detection range, which reduces the chances of misinterpretation of results. A model using regression method was also derived for the quantification of the concentration of Hg(II) in water samples. 相似文献
17.
稳定塘的扩散系数D与BOD动力学常数K的研究 总被引:2,自引:0,他引:2
扩散系数D和BOD动力学常数K对稳定塘的设计、运行与评价具有重要意义,本文通过放射性示踪法,利用闭口反应器的离散化理论示踪剂脉冲响应曲线,对试验数据进行逼近,获得扩散系数,并由此提出扩散系数的近似估算公式。稳定塘的BOD动力学常数K目前尚没有较为公认的实验测定方法,本文根据稳定塘的特点,提出了实验测定方法。 相似文献