Presence of coelenterazine in mesopelagic fishes from the Strait of Messina

Coelenterazine, a luciferine, and luciferase activity specific to coelenterazine were detected and assayed in various tissues of mid-water fishes, Argyropelecus hemigymnus, Chauliodus sloani, Myctophum punctatum, Vinciguerria attenuata and Cyclothone braueri. Coelenterazine was found mainly in digestive systems and photophores of these fishes. A large species to species variation was found: extremely high levels of coelenterazine and luciferase were found in the M. punctatum digestive system whereas only a very low level of coelenterazine was detected in C. braueri. Coelenterazine was detected in A. hemigymnus eggs supporting the hypothesis of a maternal transfer of luminous capabilities. Luciferase activity specific to coelenterazine was found in photophores as well as in various other tissues suggesting another (besides light emission) biological function for this enzymatic activity. Distribution of coelenterazine in all tissues of the individuals supports the hypothesis of the dietary acquisition of coelenterazine by these fishes.     

Observations on the nocturnal feeding of some mesopelagic decapod crustacea

Feeding in relation to temporal changes in the depth distribution of predator and prey is described for 9 species of mesopelagic decapods from an examination of 268 foreguts. Intensive nighttime feeding appears to be the rule in all species. The smaller decapods Sergestes (Sergestes) atlanticus, Sergestes (Sergestes) sargassi and Sergestes (Sergestes) pectinatus exploit the smaller prey, principally copepods and to a lesser extent ostracods. Larger decapod species Sergestes (Sergestes) henseni, Sergestes (Sergestes) curvatus, Sergestes (Sergia) grandis, Systellaspis debilis, and Acanthephyra purpurea mainly prey on macrozooplankton and micronekton, i.e., chaetognaths, euphausiids, decapods and fish, but copepods also occur in the foreguts. Gennadas valens is exceptional for the high incidence of foraminiferal remains, and a predator-prey relationship seems probable. All 9 decapod species have mixed diets, and pronounced feeding preferences are not evident. However, a high incidence of “secondary” feeding or “dietary contamination” has been deduced from the frequent occurrence of remains of the copepods Pleuromamma spp. and Oncaea spp. in the foreguts of the larger decapod species. Direct feeding cannot have occurred, since the depth distributions of these copepods and decapods are disjunct by day and night. It is concluded that the remains of Pleuromamma probably represent the food of the larger prey such as chaetognaths etc. which are eaten by the decapods. The presence of Oncaea is speculatively attributed to a possible ectoparasitic relationship with the larger prey items, but confirmatory evidence is required. These anomalies suggest that caution must be exercised in deducing predator-prey relationships simply from gut contents without consideration of distributional factors.     

Bioluminescence of deep-sea coronate medusae (Cnidaria: Scyphozoa)

Bioluminescence is the production of visible light by a living organism. The light commonly appears as flashes from point sources (involving one or more cells, usually described as photocytes) or as a glandular secretion. A visible flash usually involves synchronous light emission from a group of cells or, if from a single-celled organism such as a dinoflagellate, from a group of organelles. The number of cells (or organelles) responding synchronously is the main determinant of the flash intensity. Bioluminescence is a common phenomenon in many deep-sea animals and is widespread among the Cnidaria. In this paper, we compare and contrast in situ and laboratory recordings of the bioluminescent responses of specimens of the deep-sea scyphozoans Atolla wyvillei, Atolla vanhoffeni, Atolla parva, Nausithoe rubra, Paraphyllina intermedia, Periphyllopsis braueri and Periphylla periphylla. Displays in all seven species consist of localised flashes and propagated waves of light in the surface epithelium. The first few single waves propagate at rates of up to 60 cm s^-1 but subsequent ones in any sequence of stimuli gradually decrease in speed. After several single wave responses, a subsequent stimulus may elicit multiple waves that persist for several seconds. Following such a frenzy, the specimen becomes temporarily refractory to further stimuli, but if rested will recover its normal responses and may produce further frenzies. The dome area, situated above the coronal groove, of the genera Paraphyllina, Periphylla, and Nausithoe is covered with luminescent point sources. Such point sources are generally absent from the dome of species of Atolla. Captured specimens of A. parva also produce secretory bioluminescence, corroborating prior in situ observations of this ability. Secretory bioluminescence in P. periphylla takes the form of scintillating particles released from the lappet margins. We did not observe secretory displays in specimens of any other species in the laboratory, but one instance of apparent secretory luminescence was recorded in situ in a specimen of A. wyvillei.Communicated by J. P. Thorpe, Port Erin     

Deep-water decapod crustacean communities in the Northwestern Mediterranean: influence of submarine canyons and season

The specific composition and abundance of bathyal decapods in the Catalan Sea were investigated. A total of 109 bottom trawls were effected at depths ranging from 141 to 730 m on the continental slope in the Catalan Sea (northwestern Mediterranean) during two sampling cruises in spring and autumn 1991. Multivariate analysis of the samples revealed four groups of the decapod crustacean communities: (1) A shelf-slope transition-zone group at depths between 146 and 296 m, primarily characterized by the presence of Plesionika heterocarpus; (2) an upper-slope community between 245 and 485 m, characterized by the presence of the mesopelagic species Pasiphaea sivado and Sergestes arcticus, with Processa nouveli, Solenocera membranacea and Nephrops norvegicus as secondary species; (3) a middle-slope community below 514 m, with Aristeus antennatus and Calocaris macandreae as the most abundant species; (4) a group at 430 to 515 m, comprising all samples collected exclusively within or in the vicinity of submarine canyons. Mesopelagic decapods were predominant on the slope, while benthopelagic fishes (Merluccius merluccius, Micromesistius poutassou, Gadiculus argenteus) replaced mesopelagic decapods on the shelf. There were seasonal variations, with higher densities of mesopelagic species in spring, which were probably related, among other factors, to variations in the photoperiod. Our surveys also revealed higher species richness in the canyons together with seasonal changes in the megafaunal biomass. Generally, the upper and middle-slope communities both displayed seasonal changes in the composition and abundance of megabenthos.     

Effects of the shoot density of seagrass on fish and decapods: are correlation evident over larger spatial scales?

Two conflicting models for the organisation of assemblages of fish and decapods associated with seagrass over large spatial scales, make contradictory predictions about the relationship between density of seagrass shoots and abundance, or diversity, of animals. We tested the predictions of both models by sampling small lish and decapods associated with two species of seagrass (Zostera capricorni Aschers and Posidonia australis Hook) at up to 16 sites within several estuaries in New South Wales, Australia, for 1.5 yr (December 1988 to March 1990). Variation in density of Z. capricorni shoots explained very little of the variation in abundance of animals. However, abundance of one species, the grass shirmp Macrobrachium intermedium, was more closely related to the density of shoots during non-recruitment seasons, suggesting that predation or emigration of individuals after settlement was greater in sparse beds. The effect of variation in density of P. australis shoots was confounded with consistent distribution patterns of most fish and decapod species. As a result, data from P. australis did not provide good tests of the hypotheses. We conclude that density of seagrass shoots explained very little of the large-scale variation in abundance of associated fish and decapods. The data do, however, support the inodel which predicts that the abundance of animals among separate seagrass beds will follow the supply of new individuals to them.     

Morphological adaptations to chronic hypoxia in deep-sea decapod crustaceans from hydrothermal vents and cold seeps

Animals inhabiting hydrothermal vents and cold seeps face conditions that are challenging for survival. In particular, these two habitats are characterized by chronic hypoxia, sometimes reaching complete anoxia. The characteristics of the scaphognathite and gills were studied in four species of shrimp and three species of crabs from hydrothermal vents and cold seeps, in order to highlight potential adaptations that could enhance oxygen acquisition in comparison with shallow-water relatives. All the vent and seep species studied here exhibit significantly larger scaphognathites, likely allowing more water to flow over their gills per stroke of this appendage. This is probably more energetically efficient that prolonged hyperventilation. In contrast to annelids, vent and seep decapods usually do not possess enlarged gills, a phenomenon likely due to the physical limitations imposed by the size of the gill chamber. In the vent shrimp Rimicaris exoculata and the vent crab Bythograea thermydron, however, there is a significantly higher specific gill surface area linked to a higher number of lamellae per gram of gill. Again in contrast to annelids, the diffusion distance through the gills is not strikingly different between the vent shrimp Alvinocaris komaii and the shallow-water species Palaemon spp. This may indicate that the epithelium and cuticle of the decapod gills are already optimized for oxygen uptake and that reducing the thickness of these compartments is not physically possible without affecting the physical integrity of the gills.     

Luminescence from non-bioluminescent tissues in oceanic cephalopods

Several tissues (e.g. kidney, blood, digestive gland) in oceanic cephalopods which do not exhibit in vivo bioluminescence, luminesce when homogenized in the presence of air or when simply exposed to air in a vial (blood). The source of the luminescence appears to be a luciferin: treatment of kidney homogenates and blood with a photophore extract presumably containing luciferase resulted in a 20-fold increase in light production. Luminescence was also found in the renal fluid, which may be the source of luminescent clouds produced by squids. The variability in luminescence found in some tissues of cephalopods appeared to be related to feeding. Luminescence was also detected in the digestive glands of midwater octopods.     

Corallum morphology and composition of crustacean cryptofauna of the hermatypic coralMadracis mirabilis

The motile crustacean cryptofauna of the hermatypic coralMadracis mirabilis (Duchassaing and Michelotti) was examined from three sites on reefs along the west coast of Barbados in 1985. In addition to site differences in degree of eutrophication, two distinct with-in-corallum habitats were recognized, based on differences in the growth form of the coral host. One form grows in isolated, hemispherical heads, with short, robust, widely separated branches covered by 50 to 80% live tissue. The second form occurs within large continuous beds of long, thin, tightly spaced branches tipped by 20 to 30% live tissue. Coral habitats consisting of small isolated heads support a crustacean fauna of high decapod and amphipod diversity, with numerous rare and site endemic species and an abundance of relatively large decapods and amphipods. The continuous beds favour a community of lower decapod and amphipod diversity, fewer rare and site endemic species and with an abundance of smaller sized isopods and copepods. It is suggested that spatial separation of branches and colonies as well as food availability associated with corallum morphology are factors influencing the variation in species abundance and faunal composition of the two habitats.     

Providing a common diet to different marine decapods does not standardize the fatty acid profiles of their larvae: a warning sign for experimentation using invertebrate larvae produced in captivity

Larval decapods are commonly produced in captivity and employed in experiments to evaluate interspecific physiological and biochemical differences. Currently, it is still unknown if different decapod species provided a common diet and exposed to identical abiotic conditions produce newly hatched larvae (NHL) with similar fatty acid (FA) profiles. This study analyzed the FA composition of NHL from five marine shrimp species (Lysmata amboinensis, L. boggessi, L. debelius, L. seticaudata and Rhynchocinetes durbanensis) fed a common diet and stocked at constant temperature. FA profiles of NHL differed significantly within and among genera. NHL from species unable to molt from zoea I to zoea II in the absence of food (L. amboinensis, L. debelius and R. durbanensis) displayed the lowest FA contents. Researchers must be aware that providing a common diet to different species, even if closely related, may not standardize the FA profile of NHL produced in captivity.     

Diet and prey selectivity of scyphomedusae from Port Phillip Bay,Australia

Scyphomedusae collected from Port Phillip Bay, Victoria, Australia, between 1984 and 1986, consumed a variety of zooplankton. The percentage composition of gut contents of Cyanea capillata (Linné) in order of decreasing importance was larvaceans 31%, cladocerans 29%, fish eggs 14%, copepods 11%, hydromedusae 9%, and ascidian tadpoles 3%. The percentage composition of gut contents of Pseudorhiza haeckeli Haacke was fish eggs 41%, copepods 33%, larvaceans 8%, cladocerans 4%, crab zoea 4%, and decapod larvae 1%. Both species of scyphomedusae showed strong positive selection for fish eggs and yolk-sac larvae, and negative selection for other prey items. When fish eggs were omitted from the selectivity analyses, C. capillata showed positive selection for amphipods, decapods, crab zoea, Podon spp., larvaceans and ascidian tadpoles, and negative selection for Evadne spp. and all copepod taxa. Pseudorhiza haeckeli showed positive selection for amphipods, decapod larvae, crab zoea and cladocerans, and negative selection for cirripede larvae, larvaceans and hydromedusae. Amongst copepods, P. haeckeli showed positive selection for calanoid and harpacticoid copepods and negative selection for cyclopoid copepods.     

Concentrations of Mn,Fe, Cu,Zn and Cd in the mesopelagic decapod Systellaspis debilis from the East Atlantic Ocean

Specimens of the oceanic decapod Systellaspis debilis were collected from six sites in the East Atlantic Ocean between 1970 and 1984, and were analysed for Mn, Fe, Cu, Zn and Cd. The data confirm that there are small but significant differences in mean metal concentrations from some sites which showed no obvious pattern in relation to geographic location of the samples. As a result, ranges of site means are quoted as baseline levels for each metal (g g^-1 dry wt): 2.3 to 2.9 g Mn g^-1, 31.2 to 77.8 g Fe g^-1, 25.9 to 83.4 g Cu g^-1, 41.9 to 92.9 gZn g^-1, 11.1 to 31.8 g Cd g^-1. The concentration of cadmium in S. debilis from all sites was raised relative to cadmium concentrations reported for coastal decapods, perhaps as a result of dietary enrichment. Metal accumulation may provide useful information for understanding the complex feeding behaviour of many oceanic animals.     

Diets of deep-sea brachyuran crabs in the Western Mediterranean Sea

This study examines the feeding habits of Paromola cuvieri (Risso, 1816) and Geryon longipes A. Milne Edwards, 1881, the only two common deep-sea brachyuran crabs inhabiting the bathyal mud assemblages in the Catalan Sea (Western Mediterranean). Samples were obtained by bottom trawls at depths between 360 and 1871 m during 1983 to 1992. Both species had highly diverse diets, but very low feeding activity, as reflected by the high proportion of empty stomachs. Both characteristics may be important factors enabling deepsea crabs to adapt to bathyal zones, where trophic resources are scarce. The most important food items found in P. cuvieri were fish remains (teleost, sharks) and benthic decapods (Monodaeus couchii, Munida tenuimana). Scavenging activity plays an important role in this species. The diet of G. longipes included a broad range of benthic invertebrates. In the upper middle slope, the bivalve Abra longicallus, decapods (Calocaris macandreae and Monodaeus couchii), echinoderms and polychaetes were the dominant prey, with epibenthic peracarids as a secondary resource. On the lower middle slope, the incidence of decapod crustaceans (C. macandreae, Pontiphilus norvegicus) and peracarids in the diet declined. Small macrobenthic prey (glycerids, cumaceans or amphipods) were rare in the diet of both species, in accordance with the large size of the crab specimens studied. The absence of preferred prey items and the lack of food items of an optimum size on the lower slope may contribute to the progressive decline in abundance of P. cuvieri and G. longipes with increasing depth.     

Food division within two sympatric species-pairs of skates (Pisces: Rajidae)

Food habits of two sympatric species-pairs of skates (Raja erinacea-R. ocellata and R. radiata-R. senta), which occur off the east coast of North America were investigated. Stomachs from over 1600 specimens of the 4 species were collected during winter, summer, and autumn of 1969 and the winter of 1970. Diets of R. erinacea and R. ocellata consisted largely of amphipods, decapod crustaceans and polychaetes. However, R. ocellata consumed relatively more fishes and polychaetes and less decapods than R. erinacea. These two skates ate many of the same species but in different proportions; R. ocellata tended to feed on infauna and R. erinacea tended to feed on epifauna. Food preferences of the two species may be correlated to the difference in shape of the mouth and number of tooth rows. R. radiata and R. senta both fed heavily on decapod crustaceans and euphausids, but polychaetes were relatively more important to R. radiata and mysids were relatively more important to R. senta. R. radiata had a very diversified diet and fed on both epifauna and infauna. The diet of R. senta was very restricted and consisted almost entirely of epifauna. Diets of the two species-pairs were similar, but isopods and bivalves were more important to the R. erinacea-R. ocellata pair and euphausids and mysids were more important to the R. radiata-R. senta pair. These differences may reflect differences in the benthic communities with which the species-pairs are associated. Amount of overlap in resource utilization of the pairs of skates was compared with that of some other congeneric organisms.Virginia Institute of Marine Science Contribution No. 727.     

Growth parameters of deep-water decapod crustaceans in the Northwestern Mediterranean Sea: a comparative approach

 Relative and absolute growth were studied in 17 species of deep-water decapod crustaceans, spanning nine families of six different infra-orders, in the Northwestern Mediterranean Sea. The overall maximum abundance of these species lay between 200 m and 750 m (i.e. upper- and mid-slope species). Relative and absolute growth rates were compared by contrasting the slopes of the size–weight relationships for the different species and calculating the von Bertalanffy growth-equation parameters asymptotic length (L _∞ ) and growth rate (k). The size–weight relationships differed significantly as function of the species' life habits. The results revealed a significant decrease in weight relative to size in mesopelagic species (which carry out diel vertical migrations), an almost isometric relationship between size and weight in the less mobile nektobenthic species, and a significant increase in weight relative to size in strictly benthic species. The mean allometric coefficient for each group increased significantly from mesopelagic to benthic species. However, no general trend was observed in the growth-performance index, Φ (an index used to compare absolute growth rates between species, as a function of habit and depth of maximum abundance for all species combined), suggesting that the deep-water decapod crustaceans studied have similar absolute growth rates. Nevertheless, comparison of growth-parameter and growth-performance index values within families did reveal differences. Mesopelagic species of the families Sergestoidae and Pasiphaeidae showed slightly increased growth rates with increasing depth of distribution. Nektobenthic species of the genus Plesionika followed a trend opposite to that shown by mesopelagic species, with a higher growth rate for the shallowest-dwelling species (P. heterocarpus) than the deepest-dwelling species (P. acanthonotus). Taking growth as one of the major components of an organism's energy budget, the growth rates for the decapod crustacean species in this study were significantly lower than those reported in the literature for shallow-water penaeid crustacean species (which are distributed in higher-temperature habitats than deep-water Mediterranean crustaceans) and higher than those reported for mesopelagic myctophid fish species. Hence, the well-defined growth trends shown by deep-water decapod crustacean species in the Northwestern Mediterranean Sea, compared to the less well-defined trends in the other taxa, is discussed in the framework of the overall dynamics of their ecosystem. Received: 25 May 1998 / Accepted: 27 September 1999     

Seasonal changes of benthic megafauna in the Ría de Muros e Noia (Galicia,North-West Spain)

A community of decapod crustaceans (Brachyura) was sampled seasonally (October 1978–July 1979) from three habitats (raft, middle and beach) in the Ría de Muros e Noia (North-West Spain), with the purpose of studying spatial and temporal changes in the community and comparing with communities in the neighbouring Ría de Arousa, which supports an intense mussel (Mytilus edulis)-raft culture. The Portunidae family dominated the decapod community. Polybius henslowi, a species with pelagic stages, which enters the rías periodically in large numbers, was the dominant species at all the stations throughout the sampling period, attaining densities of 1.6 individuals m^-2 (18.7 g wet wt m^-2) in summer. Macropipus depurator and M. puber were the next most important species at the raft station, M. depurator at middle stations, and M. vernalis and Carcinus maenas at beach stations. The highest population densities were recorded in summer, due to the great abundance of Polybius henslowi at this time. When the data were reconsidered omitting p. henslowi, highest densities were in autumn and winter in the inner ría. In general, the nature of the substratum, the presence of mussel rafts, depth and salinity were the main factors determining the structure of the community. Density and biomass in the Ría de Muros e Noia (P. henslowi omitted) were lower than in the Ría de Arousa (up to six times lower in some areas). The Ría de Muros lacks the extensive number of mussel rafts present in the Ría de Arousa which constitute an important food resource for decapods.     

Some aspects of the ecology of the echiuran worm Bonellia viridis and associated infauna

In inshore waters around Malta, Bonellia viridis inhabit burrows with multiple exits in calcareous rocks, and are most abundant in areas bordering Posidonia oceanica meadows. The associated epiflora and fauna are typical of the Mediterranean hard-substrate infralittoral zone. The larger infaunal species associated with B. viridis include a poriferan, Cliona sp.; an unidentified nemertean worm; various polychaetes; the sipunculans Phascolosoma granulatum and Aspidosiphon muelleri; two species of molluscs-Lithophaga lithophaga and Lepidopleurus cajetanus; 8 species of decapod crustaceans; and the teleost Gobius geniporus. Four types of burrows are found in rocks containing B. viridis. The larger burrows (referred to herein as UBA burrows) contain 3 main species-the decapods Upogebia deltaura and Alpheus dentipes and B. viridis itself. Experimental evidence suggests that U. deltaura excavates the UBA burrows mechanically, although B. viridis may secondarily modify them by secretion of an acidic mucus and gentle mechanical action. There is a definite community of organisms living as commensals in the burrow of U. deltaura. The assemblage of organisms bears a striking parallelism to the assemblages inhabiting the burrows of species of Upogebia, Callianassa and the echiuran Urechis caupo of the Pacific coast of N. America. A food web for the UBA burrow community is suggested.     

Latitudinal trends in size-dependence of bioluminescence in the midshipman fish Porichthys notatus

The bioluminescent fish Porichthys notatus (plainfin midshipman), has a discontinuous distribution along the Pacific coast of North America. The fish is present from Cape Mendocino southward to Baja California, Mexico, absent off the coast of Oregon, USA, and abundant, northward, in Puget Sound, Washington. Interestingly, the population in Puget Sound lacks the substrate (luciferin) necessary for the luminescence reaction and, despite possessing an otherwise fully functional photophore system, is nonluminescent. The California population of P. notatus is uniformly luminescent south of Monterey Bay, but 15% of the speciments tested from San Francisco Bay and the Gulf of the Farallons have been reported to be nonluminescent. Explanations for nonluminescent midshipman in both Puget Sound and the San Francisco Bay area have focussed on a dietary requirement for luciferin. To gain further insight into reasons for nonluminescence in the San Francisco Bay region, the distribution of bioluminescence in P. notatus was studied from Monterey Bay to Cape Mendocino during 1985. A complex pattern of bioluminescence was found, in which nonluminescent individuals reflected neither a local anomaly in the San Francisco Bay region nor a simple gradient of decreasing luminescence towards the northern end of the range of the California population. Instead, a distinct size-dependent component in luminescence capability of the fish was observed. Aspects of the life history of P. notatus and related factors which might influence the bioluminescence characteristics of this population are discussed.Please address all correspondence to Dr. F.I. Tsuji at the Osaka Bioscience Institute     

Caloric values of some North Atlantic invertebrates

Nineteen marine species were analyzed for caloric value; 7 of these were analysed for seasonal trends. Variation in caloric value for the polychaetesNephtys incisa andLumbrineris fragilis was not related to season.Pandalus montagui (decapod shrimp),Leptocheirus pinguis (gammarid amphipod),Astarte undata, Arctica islandica (pelecypods) had summer maxima.Meganyctiphanes norvegica (krill) had more complex seasonal changes involving winter and summer maxima.     

Planktonic bioluminescence in the pack ice and the marginal ice zone of the Beaufort Sea

An icebreaker cruise into the Beaufort Sea in the fall of 1986 provided a unique opportunity for studying planktonic bioluminescence in ice fields and in the marginal ice zone. Bathyphotometer casts (bioluminescence intensity, seawater temperature, beam attenuation coefficient, and salinity) and biological collections were made to a depth of 100 m. A light budget, which describes the planktonic species responsible for the measured bioluminescence, and a dinoflagellate species budget were constructed from the mean light output from luminescent plankton and plankton counts. The vertical distribution of bioluminescence among the ice stations was similar. The maximum intensities were 2 to 8×10⁶ photons s^-1 cm^-3 in the upper 50 m of the sea-ice interface. The marginal ice zone station (MIZ) exhibited a maximum intensity of 2 to 3×10⁸ photons s^-1 cm^-3 between 5 and 30 m depth. At Ice Station 2, Metridia longa and their nauplii contributed approximately 80% of stimulable bioluminescence in the upper 10 m but, overall, Protoperidinium spp. dinoflagellates contributed most of the light to a depth of 100 m. In the MIZ, Protoperidinium spp. dinoflagellates contributed 90% of the light within the upper 10 m, decreasing to 43% of the contributed light at a depth of 40 m. Below 40 m, dinoflagellate bioluminescence decreased to a few percent of the total to a depth of 90 m. Metridia spp. copepods contributed more than 50% of the light at depths from 40 to 90 m. Ostracods, larvaceans, and euphausiid furcilia contributed <1% of all bioluminescence at all depths sampled. Correlation analyses between measured bioluminescence (photons s^-1 cm^-3), the number of bioluminescent dinoflagellates and the light budget for the MIZ indicated highly significant associations: r=0.919, p=0.001, and r=0.912, p<0.001, respectively (Student's two-tailed t-tests). Bioluminescence was negatively correlated with seawater salinity at all stations (p=0.001). Maximum bioluminescence was measured in the less saline surface waters at all stations.     

Deep sea benthic bioluminescence at artificial food falls, 1,000–4,800 m depth,in the Porcupine Seabight and Abyssal Plain,North East Atlantic Ocean

Natural bioluminescence (that not mechanically stimulated by human intervention) produced by organisms on the seafloor of the northeast Atlantic ocean between 970 and 4,800 m depth was examined using an image intensifying (ISIT) camera mounted on an autonomous lander system. In the absence of bait little or no luminescence was observed but with bait present there was a significant inverse relationship with depth, Log₁₀ (1 + number of events h⁻¹) = 1.7627–0.3235 depth (km) (r ² = 0.8158, P < 0.001) indicating an average of 2.6 events h⁻¹ at 4 km and 28 h⁻¹ at 1 km. But in an area at ca. 1 km depth near carbonate and coral mounds the mean was 133 events h⁻¹, much higher than predicted. In this bioluminescent hot spot 52–483 events h⁻¹ were observed including moving luminescent targets and release of patches of luminescent material into the water around the bait so that on occasions the whole area around the bait was illuminated persisting on a time scale of minutes. At abyssal depths, luminescence was much less than reported at similar depths in the tropical NE Atlantic off Cape Verde. The sources of luminescence could not be determined but in the most active areas were associated with presence of eels Synaphobranchus kaupii which although themselves not luminescent may have stimulated luminescence from prey organisms such as ostracods (Vargula norvegica).