Tail beat frequency as a predictor of swimming speed and oxygen consumption of saithe (<Emphasis Type="Italic">Pollachius virens</Emphasis>) and whiting (<Emphasis Type="Italic">Merlangius merlangus</Emphasis>) during forced swimming

Oxygen consumption and tail beat frequency were measured on saithe (Pollachius virens) and whiting (Merlangius merlangus) during steady swimming. Oxygen consumption increased exponentially with swimming speed, and the relationship was described by a power function. The extrapolated standard metabolic rates (SMR) were similar for saithe and whiting, whereas the active metabolic rate (AMR) was twice as high for saithe. The higher AMR resulted in a higher scope for activity in accordance with the higher critical swimming speed (U _crit) achieved by saithe. The optimum swimming speed (U _opt) was 1.4 BL s⁻¹ for saithe and 1.0 BL s⁻¹ for whiting with a corresponding cost of transport (COT) of 0.14 and 0.15 J N⁻¹ m⁻¹. Tail beat frequency correlated strongly with swimming speed as well as with oxygen consumption. In contrast to swimming speed and oxygen consumption, measurement of tail beat frequency on individual free-ranging fish is relatively uncomplicated. Tail beat frequency may therefore serve as a predictor of swimming speed and oxygen consumption of saithe and whiting in the field.     

Aerobic metabolic rates of swimming juvenile mako sharks, <Emphasis Type="Italic">Isurus oxyrinchus</Emphasis>

The shortfin mako shark, Isurus oxyrinchus, is a highly streamlined epipelagic predator that has several anatomical and physiological specializations hypothesized to increase aerobic swimming performance. A large swim-tunnel respirometer was used to measure oxygen consumption (MO₂) in juvenile mako sharks (swimming under controlled temperature and flow conditions) to test the hypothesis that the mako shark has an elevated maintenance metabolism when compared to other sharks of similar size swimming at the same water temperature. Specimen collections were conducted off the coast of southern California, USA (32.94°N and 117.37°W) in 2001-2002 at sea-surface temperatures of 16.0–21.0°C. Swimming MO₂ and tail beat frequency (TBF) were measured for nine mako sharks [77–107 cm in total length (TL) and 4.4 to 9.5 kg body mass] at speeds from 28 to 54 cm s⁻¹ (0.27–0.65 TL s⁻¹) and water temperatures of 16.5–19.5°C. Standard metabolic rate (SMR) was estimated from the extrapolation to 0-velocity of the linear regression through the LogMO₂ and swimming speed data. The estimated LogSMR (±SE) for the pooled data was 2.0937 ± 0.058 or 124 mg O₂ kg⁻¹ h⁻¹. The routine metabolic rate (RMR) calculated from seventeen MO₂ measurements from all specimens, at all test speeds was (mean ± SE) 344 ± 22 mg O₂ kg⁻¹h⁻¹ at 0.44 ± 0.03 TL s⁻¹. The maximum metabolic rate (MMR) measured for any one shark in this study was 541 mg O₂ kg⁻¹h⁻¹ at 54 cm s⁻¹ (0.65 TL s⁻¹). The mean (±SE) TBF for 39 observations of steady swimming at all test speeds was 1.00 ± 0.01 Hz, which agrees with field observations of 1.03 ± 0.03 Hz in four undisturbed free-swimming mako sharks observed during the same time period. These findings suggest that the estimate of SMR for juvenile makos is comparable to that recorded for other similar-sized, ram-ventilating shark species (when corrected for differences in experimental temperature). However, the mako RMR and MMR are apparently among the highest measured for any shark species.     

Effect of body mass,temperature and food deprivation on oxygen consumption rate of common cuttlefish <Emphasis Type="Italic">Sepia officinalis</Emphasis>

Predictions of short and long term changes in Sepia officinalis metabolism are useful, since this species is both economically important for aquaculture and also is an ideal experimental laboratory organism. In this study standard and routine oxygen consumption rates of newly hatched and juvenile laboratory raised cuttlefish S. officinalis ranging between 0.04 and 18.48 g dry body mass (Dm), were measured over a range of temperatures (10, 15, 20 and 25°C). The mass exponent (b) ranged between 0.706 and 0.992 for standard oxygen consumption and between 0.694 and 0.990 for routine oxygen consumption. Oxygen consumption scaled allometrically (b = 0.7) with body mass for cuttlefish <2 g Dm and isometrically (b = 1) thereafter. No significant differences were apparent amongst the slopes of oxygen consumption and body mass at different temperatures for standard and routine oxygen consumption. However, the intercepts differed significantly amongst the regression lines, indicating a significant effect of temperature on the magnitude of oxygen consumption. The combined effect of temperature (T) and dry body mass (Dm) are best described by the following equations: cuttlefish <2 g, MO₂ = 0.116Dm^0.7111.086 ^T and >2 g, MO₂ = 0.076Dm^0.9831.091 ^T for standard oxygen consumption; cuttlefish <2 g, MO₂ = 0.538Dm^0.7291.057 ^T and >2 g, MO₂ = 0.225Dm^0.9621.081 ^T for routine oxygen consumption. Using these equations it was estimated that a cuttlefish of 1 g Dm held at 20°C, eating 5% Dm day⁻¹ and undergoing standard and routine metabolism consumes 21.3 and 35.4%, respectively of its total daily energy intake. Juvenile cuttlefish (3.32–5.08 g Dm) held at 15°C and deprived of food for 27 days maintained a stable standard oxygen consumption rate for the first 6 days following starvation. By the 18th day without food, oxygen consumption rate had declined by 53% and further declined to 65% below the standard oxygen consumption rate on the 27th day. Upon resumption of feeding, the respiration rate returned immediately to the initial level prior to food deprivation. The present study defines the basic energy requirements and general physiological state of young cuttlefish at temperatures of 10–25°C with and without food.     

Prolonged exposure to low dissolved oxygen affects early development and swimming behaviour in the gastropod Nassarius festivus (Nassariidae)

Effects of low dissolved oxygen on early development and swimming behaviour of veliger larvae of the scavenging gastropod Nassarius festivus were studied. Embryonic development was significantly delayed when dissolved oxygen level was reduced to 3.0 mg O₂ l⁻¹ and no embryo hatched successfully at 0.5 mg O₂ l⁻¹. Veliger larvae hatched at 4.5 mg O₂ l⁻¹ had significantly smaller velar lobe, shell length and shell width. Median 48-h LC₅₀ value of the veliger larvae was estimated at 1.25 mg O₂ l⁻¹ with lower swimming speed (swimming velocity and dispersal velocity) being recorded for the survivors exposed to reduced oxygen levels. The percentage of veliger larvae that developed into crawling juveniles was significantly reduced and metamorphosis was delayed at 4.5 mg O₂ l⁻¹ whereas all larvae at 3.5 mg O₂ l⁻¹ died before they underwent metamorphosis. Juveniles developed at 4.5 mg O₂ l⁻¹ were also smaller than those at 6.0 mg O₂ l⁻¹. Results indicated that dissolved oxygen levels well above hypoxia levels (2.8 mg O₂ l⁻¹) have already had significant impact on the hatching success and larval development in gastropods, which may lead to long-term decreases in population growth.     

Critical swimming speeds of late-stage coral reef fish larvae: variation within species,among species and between locations

The swimming abilities of larval fishes are important for their survival, potentially affecting their ability to avoid predators, obtain food and control dispersal patterns. Near settlement swimming abilities may also influence spatial and temporal patterns of recruitment. We examined Critical speed (U-crit) swimming ability in late stage larvae of 89 species of coral reef fishes from the Great Barrier Reef and the Caribbean. Coefficients of variation in U-crit calculated at the individual level were high (28.4%), and this was not explained by differences in size or condition factor of these same larvae. Among species U-crit ranged from 5.5 cm s⁻¹ to 100.8 cm s⁻¹ (mean=37.3 cm s⁻¹), with 95% of species able to swim faster than the average current speed around Lizard Island, suggesting that most species should be capable of influencing their spatial and temporal patterns of settlement. Inter-specific differences in swimming ability (at both the family and species levels) were significantly correlated with size and larval morphology. Correlations were found between swimming performance and propulsive area, fineness ratio and aspect ratio, and these morphological parameters may prove useful for predicting swimming ability in other taxa. Overall, the swimming speeds of larvae from the same families at the two locations were relatively similar, although the Lutjanidae and Acanthuridae from the Caribbean were significantly slower than those from the great barrier reef. Differences in swimming speed and body form among late stage larvae suggests that they will respond differently to factors influencing survival and transport during their pelagic phase, as well as habitat use following settlement.     

Swimming ability of eels (<Emphasis Type="Italic">Anguilla rostrata,Conger oceanicus</Emphasis>) at estuarine ingress: contrasting patterns of cross-shelf transport?

The transport of eel early life stages may be critical to their population dynamics. This transport from ocean spawning to freshwater, estuarine and coastal nursery areas is a combination of physical and biological processes (including swimming behavior). In New Jersey, USA, the American eel (Anguilla rostrata) enters estuaries as glass eels (48.7–68.1 mm TL) in contrast to the Conger eel (Conger oceanicus) that enters as larger (metamorphosing) leptocephali (68.3–117.8 mm TL). To begin to understand the mechanisms of cross-shelf transport for these species, we measured the potential swimming capability (critical swimming speed, U _crit) under ambient conditions throughout the ingress season. A. rostrata glass eels were collected over many months (January–June) at a range of temperatures (4–21°C), with relative condition declining over the course of the ingress period as temperatures warmed. C. oceanicus occurred later in the season (April–June) and at warmer temperatures (14–24.5°C). Mean U _crit values for A. rostrata (11.7–13.3 cm s⁻¹) and C. oceanicus (14.7–18.6 cm s⁻¹) were comparable, but variable, with portions of the variability explained by water temperature, relative condition, ontogenetic stage, and fish length. Travel times to Little Egg Inlet, New Jersey, estimated using 50% U _crit values, indicate it would take A. rostrata ~30 and ~60 days to swim from the shelf edge and Gulf Stream, respectively. Travel times for C. oceanicus were shorter, ~20 days from the shelf edge, and ~45 days from the Gulf Stream. Despite differences in life stage, our results indicate both species are competent swimmers, and suggest they are capable of swimming from the Gulf Stream and/or edge of the continental shelf to estuarine inlets.     

Energetics of underwater swimming in the great cormorant (Phalacrocorax carbo sinensis)

Resting metabolic rate (RMR), energy requirements and body core temperature were measured during underwater swimming in great cormorants (Phalacrocorax carbo sinensis) at the zoological garden in Neumünster, Germany, using gas respirometry and stomach temperature loggers. We used a 13 m long still water canal equipped with a respiration chamber at each end. Birds swam voluntarily in the canal at a mean speed of 1.51 ms^-1. Power input during underwater swimming averaged 31.4 W kg^-1. Minimal costs of transport of 19.1 J kg^-1 m^-1 were observed at a speed of 1.92 m s^-1. Body core temperature was stable in all birds within the first 60 min spent in the canal. After that, body temperature dropped at a rate of 0.14°C min^-1 until the birds voluntarily left the water. Our data indicate that great cormorants spend 2.7 times more energy than Adélie penguins (Pygoscelis adeliae) during underwater swimming. This can be essentially attributed to their poor insulation, their mode of locomotion underwater and differences in streamlining. RMR on land was related to body mass via VO₂=0.691 M^0.755 (where VO₂ is O₂-consumption in litre h^-1 and M is body mass in kg). In order to quantify the effects of external devices on energy consumption during underwater swimming, we tested a dummy data logger attached to the back of the cormorants as well as a ring on the leg. The ring had no apparent influence on the swimming energetics of the cormorants. In birds equipped with dummy loggers, swimming speed was not significantly influenced, but both power input and costs of transport increased by a mean of 19% for swimming speeds between 1.4 and 1.8 m s^-1.     

Respiration rate and cost of swimming for Antarctic krill,<Emphasis Type="Italic"> Euphausia superba</Emphasis>, in large groups in the laboratory

Constructing realistic energy budgets for Antarctic krill, Euphausia superba, is hampered by the lack of data on the metabolic costs associated with swimming. In this study respiration rates and pleopod beating rates were measured at six current speeds. Pleopod beating rates increased linearly with current speed, reaching a maximum of 6 beats s^–1 at 17 cm s^–1. There was a concomitant linear increase in respiration rate, from 1.8 mg O₂ g_D^–1 h^–1 at 3 cm s^–1 to 8.0 mg O₂ g_D^–1 h^–1 at 17 cm s^–1. The size of the group tested (50, 100 and 300 krill) did not have a significant effect on pleopod beating rates or oxygen consumption (ANCOVA, F=0.264; P>0.05). The cost of transport reached a maximum of 75 J g^–1 km^–1 at 5 cm s^–1, and then decreased with increasing current speed to 29 J g^–1 km^–1. When considered in light of energy budgets for E. superba, these data indicate that the cost of swimming could account for up to 73% of total daily metabolic expenditure during early summer.Communicated by G.F. Humphrey, Sydney     

Relationship of oxygen consumption to swimming speed in Euphausia pacifica

Oxygen consumption rate was measured as a function of swimming velocity for the vertically migrating euphausiid Euphausia pacifica at two temperatures (8° and 12°C) and pressures (1 and 40 atm) typical of its bathymetric distribution. Increased swimming speed (x; mh^-1) required increased oxygen consumption (y; μl O₂ mg dry weight^-1 h^-1), described by the equation y = 0.012x + 0.64 at 8°C, and by y = 0.020x + 0.85 at 12° C. The current concept of low swimming costs of zooplankton, based on determinations of dead drag in copepods, is contradicted by our measurements. Temperature had a more profound effect on metabolism at higher swimming speeds (112 m h^-1; Q₁₀=2.8) than on standard metabolism (O m h^-1; Q₁₀=2.0), indicating that activity is more costly at higher temperatures. Pressure caused a small but significant (P>0.05) rise in the relationship of respiratory rate to swimming speed at both temperatures. The energy cost of vertical migration for E. pacifica was estimated by applying our data on oxygen consumption vs swimming speed to published observations on sonic scattering layer movement and the day-night distribution pattern of this species. Results indicate that the cost of a diel migration of 254 m, through a temperature change of 4 °C (8° to 12° C), would cancel any energetic benefit gained by time spent at the lower temperature typical of daytime depth. The act of vertical migration is energetically expensive; its cost should be thoroughly considered in attempts to describe the energetics of vertically migrating species.     

The hypoxia avoidance behaviour of juvenile Atlantic cod (<Emphasis Type="Italic">Gadus morhua</Emphasis> L.) depends on the provision and pressure level of an O<Subscript>2</Subscript> refuge

The frequency of low O₂ (hypoxia) has increased in coastal marine areas but how fish avoid deleterious water masses is not yet clear. To assess whether the presence and oxygen pressure (PO₂) level of an O₂ refuge affects the hypoxia avoidance behaviour of fish, individual Atlantic cod (Gadus morhua L.) were exposed to a range of O₂ choices in a 2-way choice chamber at 11.4°C over two different experiments. Cod in the first experiment were allowed access to a fixed O₂ refuge (fully air-saturated seawater) whilst oxygen pressure (PO₂) on the other side was reduced in steps to a critically low level, i.e. 4.3 kPa—a point where cod can no longer regulate O₂ consumption. Under these conditions, cod did not avoid any level of hypoxia and fish swimming speed also remained unchanged. In contrast, strong avoidance reactions were exhibited in a second experiment when fish were again exposed to 4.3 kPa but the safety, i.e. PO₂, of the refuge was reduced. Fish not only spent less time at 4.3 kPa as a result of fewer sampling visits but they also swam at considerably slower speeds. The presence of an avoidance response was thus strongly related to refuge PO₂ and it is unlikely that cod, and possibly other fish species, would enter low O₂ to feed in the wild if a sufficiently safe O₂ refuge was not available. It is therefore hypothesized that the feeding range of fish may be heavily compressed if hypoxia expands and intensifies in future years.     

Determination of the swimming trajectory and speed of chain-forming dinoflagellate <Emphasis Type="Italic">Cochlodinium polykrikoides</Emphasis> with digital holographic particle tracking velocimetry

The marine dinoflagellate Cochlodinium polykrikoides is a harmful and highly motile algal species. To distinguish between the motility characteristics of solitary and chain-forming cells, the swimming trajectories and speeds of solitary cells and 2- to 8-cell chains of C. polykrikoides were measured using a digital holographic particle tracking velocimetry (PTV) technique. C. polykrikoides cells exhibited helical swimming trajectories similar to other dinoflagellate species. The swimming speed increased as the number of cells in the chain increased, from an average of 391 μm s⁻¹ (solitary cells) to 856 μm s⁻¹ (8-cell chain). The helix radius R and pitch P also increased as the number of cells in the chain increased. R increased from 9.24 μm (solitary cell) to 20.3 μm (8-cell chain) and P increased from 107 μm (solitary cell) to 164 μm (8-cell chain). The free thrust-generating motion of the transverse flagella and large drag reduction in the chain-forming cells seemed to increase the swimming speed compared to solitary cells. The measured swimming speeds agreed with those from field observations. The superior motility of chain-forming C. polykrikoides cells may be an important factor for its bloom, in addition to the factors reported previously.     

Reduced n-3 highly unsaturated fatty acids dietary content expected with global change reduces the metabolic capacity of the golden grey mullet

In this study, we hypothesised that a reduction in n-3 HUFA availability for higher consumers, as expected with global change, would negatively impact the physiological performances of fish. The aim was to experimentally evaluate the effect of n-3 HUFA dietary content on cardio-respiratory performances of the golden grey mullet (Liza aurata), a microalgae grazer of high ecological importance in European coastal areas. These performances were evaluated in terms of critical swimming speed U _crit, associated oxygen consumption MO₂, post-exercise oxygen consumption and calcium fluxes in cardiomyocytes. Two replicated groups of fish were fed on a rich (standard diet, SD diet: 1.2 % n-3 HUFA on dry matter basis, DMB) or a poor n-3 HUFA (low n-3 HUFA diet, LD diet: 0.2 % n-3 HUFA on DMB) diet during 5 months and were called SD and LD groups, respectively. The results showed that the LD diet reduced growth rate as well as the aerobic capacity of L. aurata at 20 °C, suggesting that fish may have to save energy by modifying the proportion of energy allocated to energy-demanding activities, such as digestion or feeding. In addition, this LD diet induced higher levels of haematocrit and plasma osmolality, indicating a stress response at the second and third levels in that group. However, the LD diet caused a massive increase in swimming efficiency. This should improve the capacity of L. aurata to migrate and to forage over a wide area. In turn, these could then compensate for the reduction in growth rate and aerobic metabolism.     

Effects of changes in water salinity upon exercise and cardiac performance in the European seabass (<Emphasis Type="Italic">Dicentrarchus labrax</Emphasis>)

The European seabass is an active euryhaline teleost that migrates and forages in waters of widely differing salinities. Oxygen uptake (M_O2) was measured in seabass (average mass and forklength 510 g and 34 cm, respectively) during exercise at incremental swimming speeds in a tunnel respirometer in seawater (SW) at a salinity of 30 and temperature of 14°C, and their maximal sustainable (critical) swimming speed (U_crit) determined. Cardiac output (Q) was measured via an ultrasound flow probe on their ventral aorta. The fish were then exposed to acute reductions in water salinity, to either SW (control), 10, 5, or freshwater (FW, 0), and their exercise and cardiac performance measured again, 18 h later. Seabass were also acclimated to FW for 3 weeks, and then their exercise performance measured before and at 18 h after acute exposure to SW at 30. In SW, seabass exhibited an exponential increase in M_O2 and Q with increasing swimming speed, to a maximum M_O2 of 339±17 mg kg^–1 h^–1 and maximum Q of 52.0±1.9 ml min^–1 kg^–1 (mean±1 SEM; n=19). Both M_O2 and Q exhibited signs of a plateau as the fish approached a U_crit of 2.25±0.08 bodylengths s^–1. Increases in Q during exercise were almost exclusively due to increased heart rate rather than ventricular stroke volume. There were no significant effects of the changes in salinity upon M_O2 during exercise, U_crit or cardiac performance. This was linked to an exceptional capacity to maintain plasma osmolality and tissue water content unchanged following all salinity challenges. This extraordinary adaptation would allow the seabass to maintain skeletal and cardiac muscle function while migrating through waters of widely differing salinities.Communicated by S.A. Poulet, Roscoff     

Swimming behavior of juvenile anchovies (<Emphasis Type="Italic">Anchoa</Emphasis> spp.) in an episodically hypoxic estuary: implications for individual energetics and trophic dynamics

Diel swimming behaviors of juvenile anchovies (Anchoa spp.) were observed using stationary hydroacoustics and synoptic physicochemical and zooplankton profiles during four unique water quality scenarios in the Neuse River Estuary, NC, USA. Vertical distribution of fish was restricted to waters with DO greater than 2.5 mg O₂ l⁻¹, except when greater than 70% of the water column was hypoxic and a subset of fish were occupying water with 1 mg O₂ l⁻¹. We made the prediction that an individual fish would select a swim speed that would maximize net energy gain given the abundance and availability of prey in the normoxic waters. During the day, fish adopted swim speeds between 7 and 8.8 bl s⁻¹ that were near the theoretical optimum speeds between 7.0 and 8.0 bl s⁻¹. An exception was found during severe hypoxia, when fish were swimming at 60% above the optimum speed (observed speed = 10.6 bl s⁻¹, expected = 6.4 bl s⁻¹). The anchovy is a visual planktivore; therefore, we expected a diel activity pattern characteristic of a diurnal species, with quiescence at night to minimize energetic costs. Under stratified and hypoxic conditions with high fish density coupled with limited prey availability, anchovies sustained high swimming speeds at night. The sustained nighttime activity resulted in estimated daily energy expenditure over 20% greater than fish that adopted a diurnal activity pattern. We provide evidence that the sustained nighttime activity patterns are a result of foraging at night due to a lower ration achieved during the day. During severe hypoxic events, we also observed individual fish making brief forays into the hypoxic hypolimnion. These bottom waters generally contained higher prey (copepod) concentrations than the surface waters. The bay anchovy, a facultative particle forager, adopts a range of behaviors to compensate for the effects of increased conspecific density and reduced prey availability in the presence of stratification-induced hypoxia.     

Energy expenditure of swimming bottlenose dolphins (Tursiops truncatus)

The aim of our investigations was to determine, via oxygen and carbon-dioxide respirometry, how much energy dolphins (Tursiops truncatus) require when swimming at different speeds. Experiments were conducted on two female bottlenose dolphins (mean mass 162 kg) in the dolphinarium in Nuremberg Zoo, Germany, between March and August 1997. Animals were stationed in a respiration chamber for a minimum of 90 s after performing a variety of activities. We measured respiration frequency and oxygen requirements during (1) resting, (2) swimming at various velocities and (3) leaping to various heights. Resting metabolic rate of our bottlenose dolphins (2.15 W kg⁻¹) was comparable to previously published data. Metabolic rate in swimming dolphins increased to 2.47 W kg⁻¹ at 2 m s⁻¹, while leaps to 2.2 and 3 m height required a power input of 3.5 and 4 W kg⁻¹, respectively. Transport costs of swimming dolphins were lowest (1.16 J kg⁻¹ m⁻¹, corresponding to 0.12 J N⁻¹ m⁻¹) at a speed of 2.5 m s⁻¹, yielding an optimal range speed of between 1.9 and 3.2 m s⁻¹ (corresponding to minimum cost of transport ±10%). Breathing rates during all experiments correlated very well with oxygen consumption (r ² > 0.89) and could be used to derive metabolic rates in unencumbered dolphins at sea. Received: 18 December 1998 / Accepted: 27 April 1999     

The response of Atlantic cod, <Emphasis Type="Italic">Gadus morhua</Emphasis>, to progressive hypoxia: fish swimming speed and physiological stress

Atlantic cod, Gadus morhua, were exposed to a progressive stepwise decline in water oxygen pressure Fish swimming speed and indicators of primary and secondary stress (e.g. blood cortisol and lactate) were measured to assess whether a severe shift in physiological homeostasis (i.e. stress) preceded any change in behaviour or vice versa. Swimming speed increased by 18% when was reduced rapidly from 19.9 kPa to 13.2 kPa and was interpreted as an initial avoidance response. However, swimming speed was reduced by 21% at a moderate level of steady (8.4 kPa) and continued to drop by 41% under progressively deep hypoxia (4.3 kPa). Elevations in plasma cortisol and blood lactate indicated major physiological stress but only at 4.3 kPa, which corresponds to the critical oxygen tension of this species. We propose that the drop in speed during hypoxia aids to offset major stress and is adaptive for the survival of cod in extensive areas of low oxygen.     

Life in a warm deep sea: routine activity and burst swimming performance of the shrimp<Emphasis Type="Italic"> Acanthephyra eximia</Emphasis> in the abyssal Mediterranean

Measurements of routine swimming speed, tail-flip escape responses, and oxygen consumptions were made of the deep-sea shrimp Acanthephyra eximia using autonomous landers in the Rhodos Basin at depths of up to 4,400 m and temperatures of 13–14.5°C. Routine swimming speeds at 4,200 m averaged 0.18 m s^–1 or 3.09 body lengths s^–1, approximately double those of functionally similar oceanic scavengers. During escape responses peak accelerations of 23 m s^–2 or 630.6 body lengths s^–2 were recorded, with animals reaching speeds of 1.61 m s^–1 or 34.8 body lengths s^–2. When compared to shallow-water decapods at similar temperatures these values are low for a lightly calcified shrimp such as A. eximia despite a maximum muscle mass specific power output of 90.0 W kg^–1. A preliminary oxygen consumption measurement indicated similar rates to those of oceanic crustacean scavengers and shallower-living Mediterranean crustaceans once size and temperature had been taken into account. These animals appear to have high routine swimming speeds but low burst muscle performances. This suite of traits can be accounted for by high competition for limited resources in the eastern Mediterranean, but low selective pressure for burst swimming due to reductions in predator pressure.Communicated by J.P. Thorpe, Port Erin     

Swimming performance of three southwest Pacific fishes

The logarithm of stamina for each of Sardinops sagax (4 to 6 600 s), Scomber japonicus peruanus (16 to 27 000 s) and Odontestes regia (7 to 9 900 s), adjusted to a length of 10 cm, decreased linearly over swimming speeds of 31 to 82, 25 to 78 and 24 to 75 cm s^-1, respectively (19°C). The regression coefficient was -0.064 for both S. j. peruanus and O. regia and -0.049 for S. sagax. Critical swimming speed (60 min, 5 cm s^-1) for S. sagax (10cm), 32 cm s^-1, is within the range found for other species of similar length. The suggestion of a change in regression coefficient as swimming speed increased from prolonged to burst (Brett, 1964) was not supported by the results of this study.     

Scaling relationships,individual variation and the influence of temperature on maximum swimming speed in early settled stages of the turbot Scophthalmus maximus

Escape-swimming speeds (U _max) were studied in settled turbot (Scophthalmus maximus L.) reared at 18°C. Metamorphosis was complete at 4.0 cm total length (TL). U _max scaled in proportion to TL^0.74 in fish of 0.88 5o 8.00 cm TL at 18C. The scaling relationship for U _max was similar for temperatures between 13 and 23°C and could be fitted by the model: . U _max temperature-dependent, with a Q₁₀ of 1.77 over the temperature range studied. Analysis of covariance showed that U _max for farmed turbot was 14% lower than for wild fish filmed within 2 wk of capture; 3 mo after capture the average differences in escape performance were no longer significant, which suggests that the lower escape speeds of farmed fish are due to acclimation effects and not genetic stock differences. In order to assess the individual variability of U _max, 18 wild juvenile turbot [TL=6.2±0.4 cm (Week 1) to 7.5±0.5 cm (Week 17); means±SD] were maintained in individual containers at 18°C. U _max was determined weekly for 6 wk, standardised for fish length using the scaling relationship U _max=1.46 TL ^0.74, and individuals were ranked in order of performance. Temperature was reduced after 6 wk to 13°C, resulting in a significant decline in U _max from 104.0±14.4 to 87.8±12.5 cm s^-1 (means±SD). After 3 wk at 13°C U _max had increased to a level not significantly different from that at 18°C. Kendall's coefficient of concordance showed that repeatability of ranking of the experimental U _max of individuals was maintained over a 13 wk period and through temperature change. The results demonstrate that escape-swimming speeds in juvenile turbot are repeatable, individually variable, and can be modified in response to temperature acclination.     

The effect of hypoxia on behavioural and physiological aspects of lesser sandeel, <Emphasis Type="Italic">Ammodytes tobianus</Emphasis> (Linnaeus, 1785)

Lesser sandeel (Ammodytes tobianus) is abundant in near-shore areas where it is a key prey. It exhibits the behaviour of alternating between swimming in schools and lying buried in the sediment. We first determined the species’ standard metabolic rate (SMR), critical partial pressure of oxygen and maximal oxygen uptake The sandeel were then exposed to an acute stepwise decline in water oxygen pressure (18.4, 13.8, 9.8, 7.5, 5.8, 4.0, and 3.1 kPa ). Swimming speed and routine- and post-experimental blood lactate levels were measured, in addition to levels associated with strenuous exercise. The SMR was 69.0 ± 8.4 mg O₂ kg⁻¹ h⁻¹ and the about seven times as high. The was found to be 4.1 kPa. A rapid decrease (within 1 h) in from 18.4 to 3.1 kPa had no significant effect on routine swimming speed (0.9 ± 0.06 bl s⁻¹), but steady levels at the lowest (3.1 kPa) gradually reduced the swimming speed by 95% after 40 min. The routine blood lactate levels were 2.2 ± 0.6 mmol l⁻¹, while the levels in the strenuously exercised groups were significantly higher with 5.4 ± 1.6 and 5.8 ± 1.3 mmol l⁻¹. The highest levels were observed in post-experimental fish with 7.5 ± 2.7 mmol l⁻¹. We argue that, as sandeel showed no decrease in swimming speed (to offset stress) nor an increased speed to escape the hypoxia, the fish either rely on a low SMR and being a reasonable strong oxygen regulator as a mean to cope when exposed to acute hypoxia, or that the hypoxia simply developed too fast for the fish to decide on an appropriate strategy. Not showing a behavioural response may in the present case be maladaptive, as the consequence was major physiological stress which the fish however appears tolerant towards. The high routine blood lactate levels suggest that anaerobic metabolism is associated with swimming in sandeel, which may be related to the specific lifestyle of the fish where they regularly bury in the sediment.