植物根圈污染生态研究进展

阐述了植物根圈的概念，根圈三大结构主成份是（１）植物根系，（２）微生物，（３）土壤或水等，并从它们的相互关系出发讨论了国内外有关根圈污染生态的研究情况，同时提出了根圈污染生态的研究前景和意义。     

几种挺水植物对重金属锌的抗性能力及其影响因素

水生植物已被用于重金属污染水体的生态修复,但不同水生植物对重金属的抗性能力存在差异,选择对重金属抗性和吸收能力强的植物有利于提高污水生态修复效果.以4种挺水植物为材料,通过室内培养实验研究挺水植物对重金属锌的抗性能力及其影响因素,结果表明:挺水植物地上部分铁含量显著(p<0.05)低于根和根表铁氧化物胶膜上铁含量,铁主要积累在挺水植物地下部分.加入高浓度锌时,灯心草(Jancus effuses)和菖蒲(Acorus gramineus)对锌的抗性能力较强,而茭白(Zizania ceduciflora)和美人蕉(Canna indica)则较弱.挺水植物对锌的抗性能力与其根表铁氧化物胶膜含量和地上部分铁含量有关,铁氧化物胶膜可作为一个障碍层,而降低锌对植物的毒害,而植物地上铁与锌竞争植物叶中的代谢性敏感位点而降低锌对植物的毒害.灯心草对锌的抗性能力比较强与其根表铁氧化物胶膜和地上部分铁含量较高有关,灯心草可作为一种工程植物用于锌污染水体的植物修复.     

钾肥对小麦根际土壤镉的吸收及其植物毒性的影响

研究不同钾肥对Cd在根际土壤-植物系统中转移及植物有效性的影响可为农业生产提供依据。以潮褐土为供试土样,采用盆栽方法研究了氯化钾、硝酸钾、硫酸钾对小麦(Triticum aestivum L)根际环境镉的吸收转移及植物有效性的影响。结果表明,(1)KCl提高根际土中有效态Cd质量分数(wCd),K2SO4和KNO3降低根际土壤有效态wCd。(2)K2SO4处理下,小麦根wCd显著大于对照;随KNO3和KCl施入量的增加,小麦根中Cd含量逐渐升高。(3)相同处理三种钾肥比较小麦地上部干物重表现为:KNO3>KCL>K2SO4,根干物质量均低于对照。(4)钾肥的施入提高小麦体内CAT,POD酶活性。建议Cd污染土施用KNO3,尽量避免施用KCl和K2SO4。     

植物根圈微生物群落与功能特异性机制研究

植物-土壤微生物交互作用在土壤养分循环、碳固存和温室气体排放等生态过程中发挥着重要作用,而植物源有机物输入被认为是植物-微生物交互作用的纽带。根圈土壤微生物在群落结构和功能上与根圈外土壤差异显著,并存在一定的植物群落特异性。植物源有机物的高度可利用性对土壤微生物具有复杂的影响,改变着土壤生态过程。因此,揭示植物源有机物的输入对土壤微生物的影响有助于深化对植物-土壤微生物反馈作用的认识,同时为养分循环调控、肥料施用时效、作物增产和温室气体排放及生态平衡维持提供理论支持。基于国内外最新相关研究进展,综述了两大类植物源有机物(根际沉积和凋落物)的组成和输入时间对土壤微生物群落结构和特定功能(以氮循环为例)的影响机制;探讨了稳定性同位素示踪技术、分子探针技术和宏基因组学等研究方法在植物-土壤微生物交互作用中的综合应用;总结了植物生命周期内植物源有机物化学组成和输入时空差异对植物特异性土壤微生物群落的诱导机制。植物源有机物输入对微生物群落结构和功能具有重要影响,不但显著提高优势微生物群落生物量、改变微生物群落结构及相关功能、调控特定土壤微生物活性,并且其化学性质多样性决定了土壤微生物群落植物特异性。因此,植物源有机物输入是驱动植物根圈特异微生物群落结构演替与功能演变的重要因子。     

根际促生菌影响植物吸收和转运重金属的研究进展

土壤重金属污染对生态环境和人类健康造成严重危害,使得土壤重金属污染修复成为全球关注的研究热点之一。根际土壤中存在着数量和种类丰富的微生物种群,是根际环境中最重要的生物因素。重金属污染土壤中根际微生物与植物根系以及土壤形成特殊根际微环境,影响植物重金吸收、转运过程。根际促生菌通过产生植物生长激素类物质促进植物生长,改变根际微环境中重金属元素生物有效性,增加修复植物重金属吸收量,强化重金属污染土壤植物修复效率。近年来,根际促生菌强化重金属污染土壤植物修复效率相关研究文献数量迅速增加,最新研究成果表明:根际促生菌通过菌体表面活性基团吸附,诱导植物系统抗性(ISR),激活植物抗氧化酶活性,分泌高亲和性铁载体(Siderophores)增加根际铁供给量,竞争性抑制重金属元素的根系吸收,改变植物重金属的吸收、转运及胞内分布过程,抑制重金属元素向植物地上部分转运,同时增加农作物产量。文章对根际促生菌影响植物重金属吸收﹑转运最新研究进展进行综述,提出根际促生菌原位定殖,重金属元素亚细胞分布和重金属吸收、转运分子调控机制等方面的深入研究,将有助于进一步阐明重金属污染土壤植物根际促生菌-植物相互作用机制。通过根际促生菌调控农作物可食部分重金属的累积量,为实现中低污染农田安全生产与修复研究提供新思路。     

土壤多环芳烃污染根际修复研究进展

多环芳烃(polycyclicaromatichydrocarbons,PAHs)是环境中普遍存在的具有代表性的一类重要持久性有机污染物,具“三致性”、难降解性,在土壤环境中不断积累,严重危害着土壤的生产和生态功能、农产品质量和人类健康。修复土壤多环芳烃污染已成为研究的焦点。根际修复是利用植物-微生物和根际环境降解有机污染物的复合生物修复技术,是目前最具潜力的土壤生物修复技术之一。对国内外学者近年来在土壤多环芳烃污染根际修复的效果、根际修复机理和根际修复的影响因素方面的研究进展作了较系统的综述,并分别分析了单作体系、混作体系、多进程根际修复系统和接种植物生长促进菌根际修复系统对土壤多环芳烃的修复效果。指出根际环境对PAHs的修复主要有3种机制:根系直接吸收和代谢PAHs;植物根系释放酶和分泌物去除PAHs,增加根际微生物数量,提高其活性,强化微生物群体降解PAHs。并讨论了影响根际修复PAHs的环境因素如植物、土壤类型、PAHs理化性质、菌根真菌以及表面活性剂等。植物-表面活性剂结合的根际修复技术、PAHs胁迫下根际的动态调节过程、运用分子生物学技术并结合植物根分泌物的特异性筛选高效修复植物以及植物富集的PAHs代谢产物进行跟踪与风险评价将成为未来研究的主流。     

平顶山矿区矸石山周边土壤重金属污染及优势植物富集特征

为筛选出适应该地区修复土壤重金属污染的首先目标植物,通过测定河南省平顶山市某煤矿废弃地8种优势植物根际与非根际土壤及植物本身Cd、Cr、Cu、Mn和Pb含量,分析了矿区土壤重金属污染状况及优势植物对重金属元素的富集和转移能力。结果表明,(1)研究区土壤Cd、Cr、Pb、Cu污染最为严重,单因子污染指数分别达到了66.04、5.24、2.89、1.80;内梅罗综合污染指数为47.94,达到重度污染级别。(2)猪毛蒿(Artemisiascoparia)地上部分、紫马唐(Digitaria violascens)地上部分和根部Cd含量分别超标1.52、1.25和1.35倍,全叶马兰(Kalimeris integrifolia)根部、狗尾草(Setaria viridis)地上部分和根部Cr含量分别超标1.30、3.06和1.79倍,并且狗尾草地上部分Pb含量超标1.71倍。(3)苍耳(Siberia Cocklebur)对Cd和Cu的生物富集系数和生物转移系数均大于1,对Cu的生物转移系数高达4.939。狗尾草对Cd、Cr、Pb的生物转移系数均大于1,且对Cr的生物富集系数大于1,对Pb的生物转移系数高达3.883。蒙古蒿(Artemisia mongolica)对Cr和Pb的生物转移系数均大于3,且对Cd的生物富集系数大于1。猪毛蒿对Cd的生物富集系数和生物转移系数均大于1。研究表明,平顶山矿区矸石山周边土壤是受重金属Cd-Cr-Pb-Cu复合污染的土壤。苍耳、猪毛蒿、蒙古蒿和狗尾草具有较强的富集重金属的能力,可作为治理该地区环境污染的目标植物。     

玉米根系形态对土壤Cd和芘复合污染的响应

植物根系在逆境胁迫下通过改变其形态及分布来适应不利的生长环境,根系的形态变化是植物适应外界环境（特别是土壤环境）的一个重要机制。采用盆栽模拟试验探讨了Cd＋芘复合污染对两种基因型玉米（Zeamays L.）（白玉米和黑玉米）根系形态学参数（根长、根表面积、根体积、根平均直径及不同根直径等级分布）的定量化影响。与对照未污染相比,Cd＋芘复合污染显著降低了白玉米和黑玉米茎叶和根的生物量,特别是根的生物量。Cd＋芘复合污染对白玉米生物量的抑制要比对黑玉米要灵敏。与未污染相比,Cd＋芘复合污染下白玉米和黑玉米的根长稍有增长,根表面积、根平均直径和根体积减小,但两处理间差异不显著。白玉米和黑玉米每一级根直径的根长在Cd＋芘复合污染和未污染处理间没有显著差异。白玉米和黑玉米〉0.60mm的根的根表面积在Cd＋芘复合污染下显著降低。此研究结果为重金属-多环芳烃复合污染土壤植物毒理效应和植物修复技术的进一步研究奠定基础。     

石油污染对土壤-植物系统的生态效应

伴随着石油的工业化生产和利用,石油污染已经成为一个严重的环境问题.本文从土壤、植物个体、植物群落与生态系统等层次对石油污染的生态效应进行了系统总结.含有多环芳烃(Polycyclic aromatic hydrocarbons,PAHs)、苯系物(Benzene、toluene、ethylbenzene和xylene,BTEX)等多种有毒物质的石油及其化工产品进入土壤-植物系统后会发生迁移.这不仅对土壤-植物系统的组成、结构、功能和服务产生影响,也会通过食物链危害人类健康.石油污染影响土壤水分状况、孔隙度等物理性质,土壤碳含量、养分含量等化学性质,以及土壤微生物群落组成和多样性、土壤酶活性等生物学性质.通常,石油污染会对大多数植物形成氧化胁迫,影响细胞膜透性,影响光合作用等生理活动,抑制植物生长,影响植物萌发、开花和结实.由于石油污染对植物个体的不同效应以及植物个体对石油污染的不同响应,石油污染对植物群落的影响存在3种基本模式,并最终导致植物群落生物量下降,物种多样性降低,植被盖度降低.进一步影响生态系统的生产力、稳定性和健康,最终危及生态系统的功能和服务.利用微生物、植物及其联合体菌根可以对石油污染物进行生物修复和降解,添加外源营养物质和通气等措施可以强化生物修复过程.应用植被指数和红边效应等遥感方法进行石油污染的生态效应监测具有较大的潜力.总之,如何把土壤-植物系统各水平上不同指标对石油污染物的响应与石油污染的生态效应联系起来,是目前石油污染生态效应研究的重点和难点之一.因此,亟需开展多尺度的系统研究,把室内控制实验、野外控制实验和野外调查有机地结合起来,构建多层次的石油污染标志物体系,全面认识石油污染对土壤-植物系统的生态效应,为石油污染的生态风险评价、治理和控制提供理论基础和实践指导.     

玉米修复芘污染土壤的初步研究

采用60d室内盆栽试验,研究了玉米CT38(ZEAMAYSL.)对多环芳烃芘污染土壤的修复作用.结果表明,无论种植玉米土P系列、无植物对照土M系列(无植物且添加叠氮化钠的灭菌土)和对照土W系列(无植物未灭菌土)中芘的可提取浓度都随着时间的推移逐渐减少,种植玉米加快了土壤中可提取态芘浓度的下降.在芘处理浓度为10—100mg.kg-1的污染土壤中,种植玉米CT38的土壤中芘的去除率达81.9%—89.3%,分别比无植物对照土M系列和对照W系列中芘的去除率高67.5%—70.9%和26.2%—47.0%.玉米也可积累少量芘,但积累量所占芘去除量的比例不足0.3%,植物吸收不是芘去除的主要机理.种植玉米增强了土壤中脱氢酶和脲酶等酶活性,从而促进了植物-根圈微生物体系对芘的生物降解.     

Sulfur mediated heavy metal biogeochemical cycles in coastal wetlands: From sediments,rhizosphere to vegetation

• In sediments, the transformation of sulfides may lead to the release of heavy metals. • In the rhizosphere, sulfur regulates the uptake of heavy metals by plants. • In plants, sulfur mediates a series of heavy metal tolerance mechanisms. • Explore interactions between sulfur and heavy metals on different scales is needed. The interactions and mechanisms between sulfur and heavy metals are a growing focus of biogeochemical studies in coastal wetlands. These issues underline the fate of heavy metals bound in sediments or released into the system through sediments. Despite the fact that numerous published studies have suggested sulfur has a significant impact on the bioavailability of heavy metals accumulated in coastal wetlands, to date, no review article has systematically summarized those studies, particularly from the perspective of the three major components of wetland ecosystems (sediments, rhizosphere, and vegetation). The present review summarizes the studies published in the past four decades and highlights the major achievements in this field. Research and studies available thus far indicate that under anaerobic conditions, most of the potentially bioavailable heavy metals in coastal wetland sediments are fixed as precipitates, such as metal sulfides. However, fluctuations in physicochemical conditions may affect sulfur cycling, and hence, directly or indirectly lead to the conversion and migration of heavy metals. In the rhizosphere, root activities and microbes together affect the speciation and transformation of sulfur which in turn mediate the migration of heavy metals. As for plant tissues, tolerance to heavy metals is enhanced by sulfur-containing compounds via promoting a series of chelation and detoxification processes. Finally, to further understand the interactions between sulfur and heavy metals in coastal wetlands, some major future research directions are proposed.     

不同退化程度喀斯特生态系统根际土壤的养分分布特征

以贵州中部喀斯特地区由乔木林、灌木林和灌草丛等不同退化程度生态系统中的优势物种根际土壤为研究对象,测定其C、N、P全量及有效态质量分数,分析各优势物种根际土壤的养分变化。结果表明：3个生态系统中不同优势物种根际各类土壤养分质量分数变化较大,其中,土壤C、N、P养分为乔木林中园果化香根际土壤质量分数最高,白枥根际养分质量分数最低。可溶性有机碳质量分数为乔木林中最低,其平均水平不超过10 mg·kg-1。土壤磷素的供给能力为乔木林的园果化香和刺秋相对较强,其中园果化香中达到1.5%。而且优势树种间根际土壤有效态养分质量分数的差异明显高于全量养分,说明喀斯特地区根际土壤养分差异性主要受植被因素的影响,土壤有效态养分较全量养分对植被群落演替的响应更为灵敏,而且根际土壤养分质量分数和形态的变化与土壤有机碳质量分数有显著的正相关关系,这进一步说明喀斯特地区植被类型的变化对土壤养分的循环利用具有重要的影响作用。     

玉米/花生间作条件下土壤环境因子的相关性和主成分分析

玉米/花生间作具有明显的间作优势,对作物生长和产量均有促进作用。为了阐明玉米/花生间作效应机制,测定了玉米/花生间作根际土壤养分含量、酶活性和微生物数量的变化规律,并对这些环境因子进行相关性和主成分分析。结果表明,与单作玉米和单作花生相比,玉米/花生间作显著提高了根际土壤的全氮、有效氮、有效磷含量以及脲酶和酸性磷酸酶活性,促进了间作玉米根际土壤细菌和微生物总数量显著增加。相关分析表明,有效磷含量与脲酶和酸性磷酸酶活性呈极显著正相关(P<0.01),脲酶活性和酸性磷酸酶活性显著正相关(P<0.05),真菌和放线菌数量显著正相关(P<0.05)。总钾含量和p H值、蔗糖酶活性呈显著负相关(P<0.05),蛋白酶和过氧化氢酶呈极显著负相关(P<0.01)。主成分分析获得3个主成分,解析贡献率分别为48.981%,43.617%和7.402%。第一主成分主要是放线菌、真菌、有效磷等组成,第二主成分主要为有机质和p H,第三主成分主要是总氮和细菌。经标准化后计算栽培措施得分显示:间作花生得分最高1.937,其次是间作玉米1.008,两者均显著高于单作玉米和单作花生。该研究表明玉米/花生间作系统的生态环境优于单作系统,机制解析为玉米/花生间作可明显促进土壤有效氮磷含量、脲酶和酸性磷酸酶活性及微生物数量的增加,进而改善土壤微生态环境。     

Effects of exotic Spartina alterniflora on rhizosphere and endophytic bacterial community structures and diversity in roots of native mangroves北大核心CSCD

Root-associated microbial communities are very important for biogeochemical cycles of carbon, nitrogen, and sulfur in wetland ecosystems, and help to enhance the mechanisms of plant invasions. In the estuary of Jiulong River (China), Spartina alternifiora has widely invaded Kandelia obovata-dominated habitats, making it necessary to investigate the influence of rootassociated bacteria. The endophytic and rhizosphere bacterial community structures associated with selected plant species were investigated using the barcoded Illumina paired-end sequencing technique. The diversity indices of bacteria associated with the roots of S. alterniflora were higher than those of the transition stands and K. obovata monoculture. Using principal coordinate analysis with UniFrac metrics, the comparison of diversity showed that all samples could be significantly clustered into three major groups, according to the bacterial communities of origin. Four phyla, namely, Proteobacteria, Bacteroidetes, Chloroflexi, and Firmicutes, were abundant in the rhizoplane of the two salt marsh plants, whereas Cyanobacteria and Proteobacteria were the more abundant endophytic bacteria. Proteobacteria, Bacteroidetes, Chloroflexi, and Firmicutes in the rhizosphere bacteria of S. alterniflora accounted for 78.0%, 5.6%, 3.3%, and 1.6%, respectively. Members of the phyla Spirochaetes and Chloroflexi were found among the endophytic bacteria of S. alterniflora and K. obovata, respectively. Using linear discriminate analysis, we found some dominant rhizoplane and endophytic bacteria, including Pseudoalteromonadaceae, Vibrionaceae, Methylophilaceae, and Desulfovibrio, which could potentially affect the carbon, nitrogen, and sulfur cycles. Of interest was that endophytic bacteria were more sensitive to plant invasion than rhizosphere bacteria. Thus, the results provide evidence for the isolation of functional bacteria and the effects of root-associated microbial groups on S. alterniflora invasions. © 2018 Science Press. All rights reserved.     

Multiple antibiotic resistance patterns of rhizospheric bacteria isolated from Phragmites australis growing in constructed wetland for distillery effluent treatment

Susceptibility patterns of 12 different antibiotics were investigated against rhizospheric bacteria isolated from Phragmites australis from three different zones i.e. upper (0-5 cm), middle (5-10 cm), lower (10-15 cm) in constructed wetland system with and without distillery effluent. The major pollutants of distillery effluent were phenols, sulphide, heavy metals, and higher levels of biological oxygen demand (BOD), chemical oxygen demand (COD) etc. The antibiotic resistance properties of bacteria were correlated with the heavy metal tolerance (one of distillery pollutant). Twenty-two species from contaminated and seventeen species from non-contaminated site were tested by agar disc-diffusion method. The results revealed that more than 63% of total isolates were resistance towards one or more antibiotics tested from all the three different zones of contaminated sites. The multiple-drug resistance property was shown by total 8 isolates from effluent contaminated region out of which 3 isolates were from upper zone, 3 isolates from middle zone and 2 isolates were from lower zone. Results indicated that isolates from contaminated rhizosphere were found more resistant to antibiotics than isolates from non-contaminated rhizosphere. Further this study produces evidence suggesting that tolerance to antibiotics was acquired by isolates for the adaptation and detoxification of all the pollutants present in the effluent at contaminated site. This consequently facilitated the phytoremediation of effluent, which emerges the tolerance and increases resistance to antibiotics.     

南亚热带不同植被根际微生物数量与根际土壤养分状况

研究了包括尾叶桉、广东凤丫蕨、柳叶竹、大叶相思、青皮、木荷、湿地松在内的7种南亚热带不同植物植被下土壤根际微生物与根际养分状况及其相关关系。结果表明,根际环境对细菌有明显的正效应,对放线菌和真菌有正、负两方面的影响,但对根际微生物总量具有根际效应明显;在南亚热带森林生态系统中.在植物的某些生长季节,微生物的根际效应与土壤养分的根际效应一致     

利用果胶培养基分离番茄根际细菌

采用平板稀释和PCR-DGGE相结合的方法,比较了4种培养基(果胶富营养培养基PM,只含果胶一种营养的培养基PA,添加果胶的寡营养培养基YPP以及与YPP营养成分一致但不添加果胶的寡营养培养基YPG)分离番茄根际细菌的能力.结果显示:PA培养基能够分离到42种形态的细菌;YPP培养基可以分离到最高的细菌菌落数,分离获得的种类比PA少;YPP培养基分离获得细菌的种类和菌落数量比YPG培养基要多.聚类分析也显示只有果胶一种营养的PA培养基能分离到最多种类,分离获得的菌群与自然环境中微生物群落最相似.研究结果表明添加番茄根际主要分泌物——果胶到培养基中可以提高培养基分离细菌的能力.     

小麦根际土壤汞的分布和形态变化

采用根际箱试验和连续化学萃取法,结合氢化物原子吸收光谱法,研究了非根际和根际土壤汞形态分布特征及小麦(Triticum aestivum)生长期土壤汞形态变化.结果表明.非根际汞形态分布顺序为:残渣态>强有机质结合态>碳酸盐铁锰氧化物结合态>腐殖酸络合态>水溶态>交换态,根际汞形态分布为:残渣态>碳酸盐铁锰氧化态>强有机质结合态>交换态>腐殖酸络合态>水溶态.当外源汞进入土壤后,在小麦生长60 d内,植物吸收使残渣态汞和水溶态汞含量明显降低,交换态汞和碳酸盐铁锰氧化态汞含量增加.土壤汞污染程度越大,在小麦中积累的汞就越多.其生物毒性增强,环境危害加大.     

Tree species and mycorrhizal associations influence the magnitude of rhizosphere effects

Previous research on the effects of tree species on soil processes has focused primarily on the role of leaf litter inputs. We quantified the extent to which arbuscular mycorrhizal (AM) and ectomycorrhizal (ECM) tree species influence soil microbial activity and nutrient availability through rhizosphere effects. Rhizosphere soil, bulk soil, and fine roots were collected from 12 monospecifc plots (six AM and six ECM tree species) planted on a common soil at the Turkey Hill Plantations in Dryden, New York. Rhizosphere effects were estimated by the percentage difference between rhizosphere and bulk soil samples for several assays. Rhizosphere effects on soil microbes and their activities were significant for ECM species but in only a few cases for AM species. In AM tree species, microbial biomass, net N mineralization, and phosphatase enzyme activity in the rhizosphere were 10-12% greater than in bulk soil. In ECM tree species, rhizosphere effects for microbial biomass, C mineralization rates, net N mineralization, and phosphatase activity were 25-30% greater than bulk soil, and significantly greater than AM rhizosphere effects. The magnitude of rhizosphere effects was negatively correlated with the degree of mycorrhizal colonization in AM tree species (r = -0.83) and with fine root biomass (r = -0.88) in ECM tree species, suggesting that different factors influence rhizosphere effects in tree species forming different mycorrhizal associations. Rhizosphere effects on net N mineralization and phosphatase activity were also much greater in soils with pH < 4.3 for both AM and ECM tree species, suggesting that soil pH and its relation to nutrient availability may also influence the magnitude of rhizosphere effects. Our results support the idea that tree roots stimulate nutrient availability in the rhizosphere, and that systematic differences between AM and ECM may result in distinctive rhizosphere effects for C, N, and P cycling between AM and ECM tree species.     

云南松根际与非根际磷酸酶活性与磷的有效性

通过对采集自滇中红壤区域的不同林龄云南松的根际与非根际土，以及不同磷补充量下根袋法培养的距云南松幼苗不同距离土壤的磷酸酶和有效磷的测定，探讨了根际与非根际土的磷酸酶变化及其与有效磷之间的关系。云南松根际及非根际都含有大量的磷酸酶，且这些磷酸酶的活性在根际土中较高。酸性磷酸酶是云南松土壤中的主要酶类，中性磷酸酶次之。培养实验结果显示，无论是根际土、近根际土还是远根际土，外界补充的磷量越低，其酸性和中性磷酸酶的活性越高，反映出磷饥饿对磷酸酶的诱导。但磷酸酶的变化同土壤有效磷的相关性不显著。因而酶数量增加不是云南松对磷饥饿的唯一适应性机制，它的变化或许只是磷胁迫下的一种症状。