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1.
Dispersal in coral reef fishes occurs predominantly during the larval planktonic stage of their life cycle. With relatively
brief larval stages, damselfishes (Pomacentridae) are likely to exhibit limited dispersal. This study evaluates gene flow
at three spatial scales in one species of coral reef damselfish, Dascyllus trimaculatus. Samples were collected at seven locations at Moorea, Society Islands, French Polynesia. Phylogenetic relationships and gene
flow based on mitochondrial control region DNA sequences between these locations were evaluated (first spatial scale). Although
spatial structure was not found, molecular markers showed clear temporal structure, which may be because pulses of settling
larvae have distinct genetic composition. Moorea samples were then compared with individuals from a distant island (750 km),
Rangiroa, Tuamotu Archipelago, French Polynesia (second spatial scale). Post-recruitment events (selection) and gene flow
were probably responsible for the lack of structure observed between populations from Moorea and Rangiroa. Finally, samples
from six Indo-West Pacific locations, Zanzibar, Indonesia, Japan, Christmas Island, Hawaii, and French Polynesia were compared
(third spatial scale). Strong population structure was observed between Indo-West Pacific populations.
Received: 26 May 2000 / Accepted: 10 October 2000 相似文献
2.
F. Maltagliati A. P. Peru M. Casu F. Rossi C. Lardicci M. Curini-Galletti A. Castelli 《Marine Biology》2000,136(5):871-879
The genetic relationships between morphologically indistinguishable marine and brackish populations of Syllis gracilis Grube, 1840 (Polychaeta: Syllidae) were studied by means of allozyme electrophoresis. Samples of S. gracilis from marine coastal and brackish-water habitats were examined for variation at 13 presumptive loci. In addition, a sample
of the closely related species S. prolifera (Krohn, 1852) was analysed. Five loci were multiallelic in at least one population of S. gracilis and eight loci in S. prolifera. Low to moderate levels of within-population genetic variability were found, with average expected heterozygosity values
ranging from H = 0.068 (±0.043 SE) to 0.187 (±0.069 SE) in the populations of S. gracilis; higher values were found in S. prolifera (H = 0.325 ± 0.076). The presence of various private alleles indicated a marked genetic divergence among populations of S. gracilis, with Nei's genetic distances ranging from D = 0.000 to 0.833 and a highly significant F
ST value. Furthermore, evidence for strong genetic heterogeneity between two sympatric marine populations was found. UPGMA cluster
analysis and multidimensional scaling pointed out a clear genetic divergence between brackish and marine populations. At least
two genetically divergent entities occurred in marine and brackish habitats. This could be due to local adaptation of individuals
coming from marine populations to brackish habitats, but more presumably to the occurrence of a species complex within S. gracilis.
Received: 6 June 1999 / Accepted: 7 February 2000 相似文献
3.
Lauren M. Mathews 《Marine Biology》2007,152(3):645-655
Despite the apparent absence of geographic barriers, connectivity among marine populations may be restricted by, for example,
ecological or behavioral mechanisms. In such cases, populations may show genetic differentiation even over relatively small
spatial scales. Here, mitochondrial sequence data from the cytochrome oxidase I (COI) gene and seven polymorphic microsatellite
markers were used to investigate fine geographic scale population genetic structure in the snapping shrimp Alpheus angulosus, a member of the A. armillatus species complex, from collections in Florida, Jamaica, and Puerto Rico carried out from 1999 to 2005. The COI data showed
a deep divergence that separated these samples into two mitochondrial clades, but this divergence was not supported by the
microsatellite data. The COI data reflect past population divergence not reflected in extant population structure on the whole
genome level. The microsatellite data also revealed evidence for moderate population structure between populations as close
as ∼10 km, and no evidence for isolation by distance, as divergences between near populations were at least as strong as those
between more broadly separated populations. Overall, these data suggest a role for restricted gene flow between populations,
though the mechanisms that reduce gene flow in this taxon remain unknown. 相似文献
4.
Population structure of the black tiger prawn, Penaeus monodon, among western Indian Ocean and western Pacific populations 总被引:4,自引:0,他引:4
We examined the population structure of the black tiger prawn, Penaeus monodon Fabricius, 1798, in the Indo-West Pacific by analyzing the geographic distribution of elongation factor 1-alpha intron sequences from specimens collected during the winter and spring of 1997. Both the molecular phylogeny of alleles and
F-statistics indicated very strong differentiation between populations from the western Indian Ocean and western Pacific. This
pattern is concordant with other recent studies of marine species in this region, implying that the Indo-Australian Archipelago
represents a biogeographic break between populations in the Indo-West Pacific. F
ST-values among populations in the western Indian Ocean also indicate structure within this region, whereas no structure was
found among western Pacific populations. Nucleotide diversity was significantly lower in the western Indian Ocean populations
than in the western Pacific, implying that the populations have regional differences in demographic history.
Received: 16 November 1998 / Accepted: 26 May 1999 相似文献
5.
Thomas Remerie Tine Bourgois Danny Peelaers Andy Vierstraete Jacques Vanfleteren Ann Vanreusel 《Marine Biology》2006,149(3):465-481
The phylogeographic patterns among populations of Mesopodopsis slabberi (Crustacea, Mysida), an ecological important mysid species of marine and estuarine habitats, were analysed by means of DNA sequencing of a fragment of the mitochondrial cytochrome c oxidase subunit I (COI) and the 16S ribosomal RNA genes. Samples of M. slabberi collected from five Atlantic and two Western Mediterranean populations were investigated. Very high levels of within-population molecular diversity were observed in all samples (mean h=0.807 and π=0.0083), with exception of the Mediterranean Ebro population which contained only one haplotype. Differentiation among populations was high, and a clear phylogeographic break was observed between the Atlantic and Mediterranean populations. Moreover, a strong differentiation was detected between both populations in the Western Mediterranean Sea (Alicante and Ebro delta), while two divergent lineages occurred in sympatry within the Atlantic Mondego estuary. The high congruence between both the COI and 16S rRNA sequence data, the reciprocal monophyly of the different mitochondrial clades and the levels of nucleotide divergence between them suggest the presence of a complex of cryptic species within M. slabberi. Estimations of divergence time between the different mitochondrial lineages indicate that a split occurred during the late Miocene/early Pliocene. Such a divergence could be concordant with vicariant events during sea-level drops within the Mediterranean region at that time. However, within the Mediterranean Sea, the potential of divergence through ecological diversification cannot be ruled out. 相似文献
6.
Although numerous coastal marine species show intra-specific lineage splitting and population divergence times that date to
the period of glacial cycles during the Pleistocene epoch, reported instances of recent speciation in the coastal marine environment
are relatively rare. Marine organisms with brood-protection and other reproductive modes with limited dispersal potential
have been suggested to experience more frequent speciation and extinction events than related species with higher dispersal
rates, but few studies have actually estimated divergence times of sister species in these organisms. Here, two mitochondrial
gene regions (cytochrome oxidase subunit I, putative control region and upstream tRNAs) and a nuclear gene region (Elongation
factor 1α subunit intron 4) provide evidence of recent (0.5–1.2 Mya) cladogenetic events in four pairs of putative sister
taxa in a predominantly North Pacific brooding subgenus of sea stars (Leptasterias subgenus Hexasterias). Calibration is obtained from a trans-arctic migration in a related clade of sea stars (Leptasterias subgenera Hexasterias and Nesasterias) that is timed to the opening of the Bering Strait at 3.5 ± 0.25 Mya, and uncertainty in the calibration point is accommodated
with a normally-distributed Bayesian prior probability. Similar estimates of population splitting times for two of the pairs
of putative sister taxa were obtained by a multilocus coalescent analysis. Estimates of mitochondrial mutation rates (0.01/My)
were approximately 50% of the values calibrated for sister species pairs in tropical sea stars and sea urchins.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
7.
The shortjaw mudsucker, Gillichthys seta, an intertidal goby endemic to the Sea of Cortez, has been proposed to be the paedomorphic derivative of the longjaw mudsucker,
Gillichthys mirabilis. G. mirabilis is a disjunct species, with populations found along the Pacific coast of central California to central Baja California, and
with isolated populations found in the northern Sea of Cortez. Previous studies have suggested that the endemic paedomorph
form speciated in sympatry with the Sea of Cortez population of G. mirabilis. Alternatively, this speciation event could have occurred before the separation of G. mirabilis populations into two disjunct entities. To test these alternative hypotheses, we collected adult individuals from both species
throughout their ranges from December 1997 to November 1998. We amplified and sequenced 142 partial [527 base pairs (bp)]
mitochondrial cytochrome b regions and 18 nuclear creatine kinase introns (140 bp). We found that Pacific populations of G. mirabilis separated into two distinct clades, possibly reflecting a phylogeographic break found in other fish species along the Baja
California coast at Punta Eugenia. These two Pacific populations were well separated from Sea of Cortez populations. Furthermore,
our results indicate that the split between Sea of Cortez and Pacific populations of G. mirabilis occurred well after the speciation event that separated G. mirabilis from its paedomorphic counterpart, G. seta.
Received: 30 March 2000 / Accepted: 4 September 2000 相似文献
8.
J. R. Weinberg V. R. Starczak C. Mueller G. C. Pesch S. M. Lindsay 《Marine Biology》1990,107(2):205-213
Low dispersal and sexual selection are characteristic of the coastal polychaeteNereis acuminata Ehlers 1868 [also known asNereis arenaceodentata Moore 1903 andNereis (Neanthes) caudata Delle Chiaje 1841]. We assessed levels of premating isolation between populations of this polychaete. Four North American populations were used, two from the Atlantic and two from the Pacific. Worms from all sites (1) were collected in 1987 and 1988 from the same habitat type, (2) were morphologically similar and keyed out asN. acuminata, and (3) reproduced monogamously and exhibited male parental care, an extremely rare reproductive mode in marine invertebrates. There was no evidence from 10-min or 36-h trials of premating isolation between the two Pacific populations. Incomplete premating isolation was found between the two Atlantic populations. High aggression and non-pairing occurred in some 10-min trials between males and females. However, in 36-h trials males and females from the two Atlantic populations always paired to mate. Complete premating isolation was found between Atlantic and Pacific populations. During 10-min trials, males and females from different oceans often attacked and then avoided each other, and they never paired to mate. Nor did they pair to mate in longer, 36-h trials. One Pacific and one Atlantic population were compared for tolerance to cold temperature. Pacific individuals were less able to tolerate cold water than Atlantic individuals. Two Atlantic populations studied had karyotypes with 11 pairs of small acrocentric chromosomes (2n=22), while the two Pacific populations had nine pairs of large metacentric or submetacentric chromosomes (2n=18). Such extreme dissimilarity in karyotype was not expected considering the similarity in morphology, habitat, and reproductive mode. Results suggest strongly that the Atlantic and Pacific populations have been allopatric for a long time, and are different species. 相似文献
9.
The cobblerCnidoglanis macrocephalus (Valenciennes) is an endemic marine and estuarine catfish from southern Australia. Conflicting views on the degree of isolation of the estuarine populations underscore general questions about genetic divergence in coastal species. Although estuaries are widely recognized as ecologically important, little work has been done on their role in favouring genetic divergence. In order to estimate the extent of genetic subdivision among nearshore marine and estuarine populations, electrophoretic variation of enzymes was examined in seven marine and six estuarine populations of cobbler from sites spanning 1500 km along the southwest Australian coastline. Among all populations, the mean standardized variance in allelic frequencies (F
ST) for six polymorphic loci was 0.277, a high value comparable to those of other shallow-water teleosts whose life-history characteristics and habitat preferences restrict their dispersal capability. The pattern of genetic identities between populations showed divergence between west and south coast sites. Within these regional groups, however, there was substantial heterogeneity, much of which was associated with estuaries. Among all six estuarine sites, the averageF
ST was 0.333, 40% higher than the value of 0.237 for the marine sites. Low estimates of the genetically effective number of migrants suggest population subdivision between marine and estuarine environments and between similar habitat types. This study indicates the importance of habitat in affecting the connectedness of populations, even in apparently open marine systems. 相似文献
10.
The patchy distribution of rocky intertidal communities in the tropical eastern Pacific (TEP) may impose severe constraints
on the genetic connectivity among populations of marine invertebrates associated with this habitat. In this study, we analyzed
a portion of the mitochondrial cytochrome c oxidase subunit I (COI) gene in two sympatric species of marine snails, Nerita scabricosta and Nerita funiculata, common inhabitants of the rocky intertidal from the Gulf of California (Sea of Cortez) and outer Pacific coast of the southern
Baja California (Baja) peninsula to northern South America, to assess genetic connectivity among populations of each species.
One of our aims was to determine whether the morphological, behavioral, and ecological differences observed among populations
of both species throughout their range in the TEP corresponded to population genetic differences. In addition, we were interested
in elucidating the demographic history of both species. We found no evidence of genetic structure throughout the Gulf of California
and outer coast of the Baja peninsula region for either species. Comparisons between Gulf of California/Baja and Panama populations,
however, showed significant genetic differentiation for N. scabricosta, but not for N. funiculata. The genetic differences between Mexican and Panamanian populations of N. scabricosta were consistent with previously reported ecological and behavioral differences for this species between these two distant
regions. However, previously reported size differences between northern and central/southern Gulf of California individuals
of N. scabricosta do not correspond with our findings of genetic connectivity among these populations. Results from neutrality tests (Tajima’s
D and Fu’s F
S), the mismatch distribution, and Bayesian skyline analyses suggested that both species have experienced dramatic population
expansions dating to the Pleistocene. 相似文献
11.
Yeasts were isolated from twelve established sites in the North Sea from 1964 to 1966. A percentage frequency of 99% with populations varying from <10 to >3000 viable cells/L was observed. This mycota was characterized by considerable spatial and temporal fluctuation, with the dominant yeast present being the ascosporogenous species, Debaryomyces hansenii. This taxon, as well as other common North Sea yeasts, e.g., Rhodotorula rubra and Candida diddensii, have been reported frequently from other marine locales. Noteworthy concentrations of yeasts, especially D. hansenii, were observed during summer months, often in association with various stages of development of the dinoflagellate, Noctiluca miliaris. The population dynamics of the North Sea yeasts are discussed in relation to similar studies of other marine environments. 相似文献
12.
Blue mussels in the genus Mytilus first arrived in the Atlantic Ocean from the Pacific during the Pliocene, following the opening of the Bering Strait. Repeated
periods of glaciation throughout the Pleistocene led to re-isolation of the two ocean basins and the allopatric divergence
of Mytilus edulis in the Atlantic and M. trossulus in the Pacific. Mytilus trossulus has subsequently colonized the northwest Atlantic (NW Atlantic) so that the two species are presently sympatric and hybridize
throughout much of the Canadian Maritimes and the Gulf of Maine. To estimate when M. trossulus arrived in the NW Atlantic, we have examined sequence variation within a portion of the female mtDNA lineage large untranslated
region (F-LUR) for 156 mussels sampled from three Pacific and eleven Atlantic populations of M. trossulus. Although we found no evidence of reciprocal monophyly for Pacific and NW Atlantic M. trossulus, limited gene flow between ocean basins has led to the divergence of unique sequence clades within each ocean basin. In contrast,
relative genetic homogeneity indicates high levels of gene flow within each basin. Coalescence-based analysis of the F-LUR
sequences suggests that M. trossulus recolonized the NW Atlantic from the northeast Pacific subsequent to a demographic expansion in the Pacific that occurred
~96,000 years before present (ybp). Estimates of timing of divergence for Pacific and NW Atlantic populations and the time
since expansion among NW Atlantic sequence clades indicate that M. trossulus arrived in the NW Atlantic more recently, between 20,000 and 46,000 ybp. Given that these estimates overlap with the dates
of peak ice in the NW Atlantic during the last glacial maximum (LGM, ~18,000–21,000 ybp), we suggest that colonization of
the NW Atlantic by M. trossulus occurred during, but more likely just subsequent to, the LGM and was followed by rapid temporal and spatial expansion in
the region. 相似文献
13.
Mitochondrial d-loop sequences were analyzed to characterize the phylogeographic and population genetic structure of the
northern clingfish (Gobbiesox maeandricus Girard). Sequence analysis of 378 bp from 111 individuals sampled in 14 localities along the northeast Pacific coast and
within the Strait of Georgia from 1996 to 1999 revealed marked genetic differentiation (Φct=0.247) among regional population groupings. The gene genealogy distinguished two major clades of haplotypes separated by
at least 1.1% sequence divergence. One clade with very low haplotype diversity (h=0.2095, n=18) occurred only within the recently unglaciated Strait of Georgia. The other clade had high haplotype diversity (h=0.8808, n=93) and was found in all populations. High haplotype diversity was found in open coastal populations, both north and south
of the maximum extant of the Wisconsin ice sheet, suggesting that the clingfish range was not pushed to a southern refugium
during the last glacial maximum. A nested clade analysis also did not detect a large northward expansion from a single southern
refugium. The level of sequence divergence and coalescent-based analyses suggest that the observed patterns of polymorphism
are the result of Pleistocene diversification within multiple refugia, followed by population expansion and asymmetrical lineage
introgression.
Received: 5 February 2000 / Accepted: 31 August 2000 相似文献
14.
Relatively few insects have invaded the marine environment, and only five species of sea skaters, Halobates Eschscholtz (Hemiptera: Gerridae), have successfully colonized the surface of the open ocean. All five species occur in the
Pacific Ocean, H. germanus White also occurs in the Indian Ocean, whereas H. micans Esch- scholtz is the only species found in the Atlantic Ocean. We sequenced a 780 bp long region of the mitochondrial cytochrome
oxidase subunit I gene (COI) for a total of 66 specimens of the five oceanic Halobates species. Our purpose was to investigate the genetic variation within species and estimate the amount of gene flow between
populations. We defined 27 haplotypes for H. micans and found that haplotype lineages from each of the major oceans occupied by this species are significantly different, having
sequences containing five to seven unique base substitutions. We conclude that gene flow between populations of H. micans inhabiting the Atlantic, Pacific, and Indian Ocean is limited and hypothesize that these populations have been separated
for 1 to 3 million years. Similarly, there may be limited gene flow between H. germanus populations found in the Pacific and Indian Ocean and between H. sericeus populations inhabiting the northern and southern parts of the Pacific Ocean. Finally, we discuss our findings in relation
to recent hypotheses about the influence of oceanic diffusion on the distribution and population structure of oceanic Halobates spp.
Received: 29 July 1999 / Accepted: 23 November 1999 相似文献
15.
Escolar (Lepidocybium flavobrunneum) is a large, mesopelagic fish that inhabits tropical and temperate seas throughout the world, and is a common bycatch in
pelagic longline fisheries that target tuna and swordfish. Few studies have explored the biology and natural history of escolar,
and little is known regarding its population structure. To evaluate the genetic basis of population structure of escolar throughout
their range, we surveyed genetic variation over an 806 base pair fragment of the mitochondrial control region. In total, 225
individuals from six geographically distant locations throughout the Atlantic (Gulf of Mexico, Brazil, South Africa) and Pacific
(Ecuador, Hawaii, Australia) were analyzed. A neighbor-joining tree of haplotypes based on maximum likelihood distances revealed
two highly divergent clades (δ = 4.85%) that were predominantly restricted to the Atlantic and Indo-Pacific ocean basins.
All Atlantic clade individuals occurred in the Atlantic Ocean and all but four Pacific clade individuals were found in the
Pacific Ocean. The four Atlantic escolar with Pacific clade haplotypes were found in the South Africa collection. The nuclear
ITS-1 gene region of these four individuals was subsequently analyzed and compared to the ITS-1 gene region of four individuals
from the South Africa collection with Atlantic clade haplotypes as well as four representative individuals each from the Atlantic
and Pacific collections. The four South Africa escolar with Pacific mitochondrial control region haplotypes all had ITS-1
gene region sequences that clustered with the Pacific escolar, suggesting that they were recent migrants from the Indo-Pacific.
Due to the high divergence and geographic separation of the Atlantic and Pacific clades, as well as reported morphological
differences between Atlantic and Indo-Pacific specimens, consideration of the Atlantic and Indo-Pacific populations as separate
species or subspecies may be warranted, though further study is necessary. 相似文献
16.
Allozyme variation in global populations of striped mullet, Mugil cephalus (Pisces: Mugilidae) 总被引:5,自引:0,他引:5
The striped mullet, Mugil cephalus Linnaeus, 1758, is one of the few species of marine shore fish with a worldwide circumtropical distribution. Because of this
distribution and the dependency of M. cephalus on coastal waters during various phases of its life cycle, as well as nearshore living habits, questions have been raised
regarding levels of genetic divergence and gene flow among transoceanic populations. To cast more light on this, allozyme
variation at 27 presumptive gene loci was investigated in ten globally diverse populations. The observed heterozygosity ranged
from 0.018 (Hawaii) to 0.081 (Florida), averaging 0.050. The proportion of polymorphic loci showed a similar trend. Several
populations were characterised by fixed allelic differences. Estimated gene diversities were very high, the allele frequency
variation among populations was found to be 68%; genetic distances reached 0.242, with an average of 0.117. Estimated rates
of gene flow were high among Mediterranean populations (Nm = 7.26), and between Mediterranean and East Atlantic populations (Nm= 2.86), but extremely low between non-contiguous populations within the Indian, Pacific and Atlantic Oceans, where Nm ranged from 0.03 to 0.05.
Received: 7 April 1997 / Accepted: 4 February 1998 相似文献
17.
Adaptive Units for Conservation: Population Distinction and Historic Extinctions in the Island Scrub-Jay 总被引:1,自引:0,他引:1
Abstract: The Island Scrub-Jay ( Aphelocoma insularis ) is found on Santa Cruz Island, California, and is the only insular bird species in the continental United States. We typed seven microsatellite loci and sequenced a portion of the mitochondrial DNA control region of Island Scrub-Jays and their closest mainland relative, the Western Scrub-Jay ( Aphelocoma californica ), to assess levels of variability and effective population size and to examine the evolutionary relationship between the two species. The estimated female effective population size, N ef , of the Island Scrub-Jay was 1603 (90% confidence interval: 1481–1738) and was about 7.5% of the size of the mainland species. Island and Western Scrub-Jays have highly divergent control-region sequences, and the value of 3.14 ± 0.09% sequence divergence between the two species suggests a divergence time of approximately 151,000 years ago. Because the four northern Channel Islands were joined as one large island as recently as 11,000 years ago, extinctions must have occurred on the three other northern Channel islands, Santa Rosa, San Miguel, and Anacapa, highlighting the vulnerability of the remaining population. We assessed the evolutionary significance of four island endemics, including the Island Scrub-Jay, based on both genetic and adaptive divergence. Our results show that the Island Scrub-Jay is a distinct species of high conservation value whose history and adaptive potential is not well predicted by study of other island vertebrates. 相似文献
18.
Population genetics of Crassostrea ariakensis in Asia inferred from microsatellite markers 总被引:1,自引:0,他引:1
Jie Xiao Jan F. Cordes Haiyan Wang Ximing Guo Kimberly S. Reece 《Marine Biology》2010,157(8):1767-1781
Crassostrea ariakensis is an important aquacultured oyster species in Asia, its native region. During the past decade, consideration was given to
introducing C. ariakensis into Chesapeake Bay, in the United States, to help revive the declining native oyster industry and bolster the local ecosystem.
Little is known about the ecology and biology of this species in Asia due to confusion with nomenclature and difficulty in
accurately identifying the species of wild populations in their natural environment. Even less research has been done on the
population genetics of native populations of C. ariakensis in Asia. We examined the magnitude and pattern of genetic differentiation among 10 wild populations of C. ariakensis from its confirmed distribution range using eight polymorphic microsatellite markers. Results showed a small but significant
global θ
ST (0.018), indicating genetic heterogeneity among populations. Eight genetically distinct populations were further distinguished
based on population pairwise θ
ST comparisons, including one in Japan, four in China, and three populations along the coast of South Korea. A significant positive
association was detected between genetic and geographic distances among populations, suggesting a genetic pattern of isolation
by distance. This research represents a novel observation on wild genetic population structuring in a coastal bivalve species
along the coast of the northwest Pacific. 相似文献
19.
This paper reports data on 28 allozyme loci in wild and artificially reared sea bass (Dicentrarchus labrax) samples, originating from either coastal lagoon or marine sites in the Mediterranean Sea. F
ST analysis (θ estimator) indicated strong genetic structuring among populations; around 34% of the overall genetic variation
is due to interpopulation variation. Pairwise θ estimates showed that, on average, the degree of genetic structuring was much
higher between marine populations than between samples from lagoons. Six polymorphic loci showed differences in allele frequencies
between marine and lagoon samples. Multivariate analyses of individual allozymic profiles and of allele frequencies suggested
that different arrays of genotypes prevail in lagoons compared to marine samples, particularly at those loci that, on the
basis of previous acclimation experiments, had been implicated in adaptation to freshwater. On the other hand, variation at
“neutral” allozyme loci reflects to a greater extent the geographic location of populations. Allozyme differentiation was
also studied in a D. labrax population from the Portuguese coast. Average genetic distance between this population and the Mediterranean populations
was quite high (Nei's D = 0.236) and calls into question the taxonomic status of the Portuguese population. Finally, genetic relationships between
D. labrax and D. punctatus were evaluated. Average Nei's D was 0.648, revealing high genetic differentiation between the two species, even for two sympatric populations of these species
in Egypt; thus gene flow was not indicated between species.
Received: 24 October 1996 / Accepted: 27 November 1996 相似文献
20.
Coelomactra antiquata is a commercially important bivalve species, but has been suffering from severe population decline due to over-exploitation
and the deterioration of environmental conditions. Previous genetic survey of C. antiquata conducted with allozymes combined with morphology revealed high levels of genetic differentiation between northern and southern
populations which suggests a cryptic species might exist in C. antiquata. To test this hypothesis, amplified fragment length polymorphisms (AFLPs) and 16S rRNA gene sequence were used to re-evaluate
the spatial genetic structure of six populations of C. antiquata along the coast of China. Both genetic markers display a sharp genetic break between the four northern populations (northern
lineage) and two southern population (southern lineage). Large numbers of private alleles (AFLP) were found within the northern
or southern populations and a deep divergence of about 6.5% in 16S rRNA gene sequence between the northern and southern lineages
suggests the occurrence of potential cryptic or sibling species of C. antiquata. Applying previously published rates of mutation, divergence between the two lineages is estimated to have occurred approximately
3 million years ago and may be due to allopatric isolation during the middle Pliocene times. While no genetic differentiation
was found within the northern or southern populations in both AFLP and 16S mtDNA markers, the results indicate that the northern
and southern lineage should be managed separately and any translocation between the two areas should be avoided. 相似文献