共查询到20条相似文献,搜索用时 31 毫秒
1.
Anna Suhhova Maie Bachmann Jaanus Lass Deniss Karai Hiie Hinrikus 《The Environmentalist》2009,29(2):210-214
This study is aimed to investigation of the effect of modulated 450 MHz microwave radiation on the EEG inter-hemispheric asymmetry.
Ten cycles of the microwave exposure (1 min off and 1 min on) at fixed modulation frequency were applied on two groups of
healthy volunteers. The first group of 13 subjects was exposed to microwave radiation modulated at 14 Hz and the second group
of 15 subjects at 40 Hz frequency. The peak specific absorption rate (SAR) average over 1 g was 0.303 W/kg. Differences in
SAR between hemispheres were up to 20 dB. Rod antenna was located from the left side of the head. Differences of relative
changes in EEG energy between symmetric channels FP1–FP2, T3–T4, P3–P4 and O1–O2 in exposed and sham conditions were analysed.
The results showed increase in EEG energy from the left side caused by microwave exposure. Statistical analysis done for the
whole group of subjects didn’t reveal significant differences in inter-hemispheres asymmetry between exposed and sham conditions.
However, statistical analysis performed for individual subjects detected significant differences in asymmetry caused by exposure
for 15–35% of individuals. 相似文献
2.
Maie Bachmann Ruth Tomson Jaan Kalda Maksim Säkki Jaanus Lass Viiu Tuulik Hiie Hinrikus 《The Environmentalist》2007,27(4):511-517
This study was aimed to investigate the changes in the human electroencephalographic (EEG) signal caused by modulated low-level
microwaves. The 450 MHz microwave exposure modulated at 40 Hz and 70 Hz frequencies was applied to a group of 15 volunteers.
The field power density at the scalp was 0.16 mW/cm2. Ten cycles of the exposure (1 min on and 1 min off) at both modulation frequencies were applied. Analysis of the EEG signal
was performed using three different methods: nonlinear method of scaling analysis for length distribution of low variability
periods (LDLVP), relative changes in EEG energy (S-parameter) and beta ratio (H-parameter). The analysis revealed significant changes caused by microwave for the whole group (H-parameter method). The exposure caused increase of the EEG beta power (S-parameter method). Statistically significant changes in EEG were detected for four subjects (26.7%) at 40 Hz modulation frequency
(LDLVP method). 相似文献
3.
Summary This study focuses on an origin of interaction mechanism of microwave radiation with nervous system—quasi-thermal field effect.
The microwave field can cause fluctuations and vibration of the charged particles and membranes in tissues. The hypothesis
is, that this phenomenon is similar to the effect caused by Brown motion initiated by temperature and results in the same
effects without rise in temperature. The electric field of 1 V/cm can introduce disturbance of the thermal equilibrium inside
a cell of 10 μm radius, which is equivalent to disturbance produced by temperature rise of 1 K. The hypothesis, that microwave
heating should cause an effect independent of the microwave modulation frequency, while field effect depends on modulation
frequency, was examined experimentally. The 450 MHz microwave radiation, modulated at 7, 14 and 21 Hz frequencies, power density
at the skin 0.16 mW/cm2, was applied. The experimental protocol consisted of two series of five cycles of the repetitive microwave exposure at fixed
modulation frequencies. Relative changes in EEG theta, alpha and beta rhythms of the group of 13 healthy volunteers were analysed.
Analysis of the experimental data shows that: (1) statistically significant changes in EEG rhythms depend on modulation frequency
of the microwave field; (2) microwave stimulation causes an increase of the EEG energy level; (3) the effect is most intense
at beta1 rhythm and higher modulation frequencies. These findings confirm the quasi-thermal origin of the effect, different
from average heating. 相似文献
4.
Maie Bachmann Hiie Hinrikus Kaire Aadamsoo Ülle Võhma Jaanus Lass Jekaterina Rubljova Anna Suhhova Viiu Tuulik 《The Environmentalist》2007,27(4):505-510
This study was aimed to evaluate differences in the effect of microwave exposure on patients with depressive disorder and
healthy subjects. Our experiments were carried out on a group of depressive patients (women, 18 subjects) and comparison group
of healthy volunteers (women, 18 subjects) exposed during 30 min to 450 MHz microwave radiation modulated at 1,000 Hz frequency.
The field power density at the scalp was 0.9 mW/cm2. As a subjective criteria of microwave effect, the Brief Affect Scale (BAS) and Visual Analogue Scale (VAS) before and after
each exposure procedure were used. The analysis of EEG was performed and ratio of the EEG beta and theta power was selected
as a measure for evaluation of the microwave effect. The BAS and VAS revealed rather improvement in subjective mood score
after exposure for majority of depressive subjects (11) and no changes for others (7). The EEG analysis detected differences
between calculated parameters for exposed and sham recordings in depression as well as healthy group. Statistically significant
changes were introduced by microwave for five patients with depressive disorder and for one healthy subject. The rate of subjects
affected by microwave in depression group (28%) was five times higher compared to that rate in healthy group (5.6%). 相似文献
5.
Maie Bachmann Maksim Säkki Jaan Kalda Jaanus Lass Viiu Tuulik Hiie Hinrikus 《The Environmentalist》2005,25(2-4):165-171
Summary This study focuses on discrimination of changes, produced by low-level microwave exposure in intensity and time variability
of the human EEG at rest. The power spectral density (PSD) method and nonlinear scaling analysis of the length distribution
of low variability periods (LDLVP) were selected for analysis of the EEG signal. During the study, 19 healthy volunteers were
exposed to a microwave (450 MHz) of 217 Hz frequency on-off modulation. The field power density at the scalp was 0.16 mW/cm2. The experimental protocol consisted of ten cycles of repetitive microwave exposure. Signals from frontal, temporal, parietal
and occipital EEG channels on EEG theta, alpha and beta rhythms were analysed. Exposure to microwave causes average increase
of EEG activity. LDLVP analysis discriminated significant effect in time variability for 2 subjects (11%). PSD method detected
significant changes in intensity for 4 subjects (21%). The effect of low-level microwave exposure is stronger on EEG beta
rhythm in temporal and parietal regions of the human brain. 相似文献
6.
Eleni Nanou Vassilis Tsiafakis E. Kapareliotis Charalabos Papageorgiou Andreas Rabavilas Christos Capsalis 《The Environmentalist》2005,25(2-4):173-179
Summary The effects of electromagnetic fields (EMF) emitted by cellular phones on human electroencephalogram (EEG) were studied during
an auditory memory task. The experimental method and the experimental setup are introduced as a credible measurement method
of EEG. 19 normal subjects (10 women and 9 men) performed the memory task both with and without exposure to a 900 MHz signal,
emitted by a dipole antenna placed near the subjects' head. The energy of the EEG signals was calculated at the time domain.
A Fourier transform of the EEG signals was done and the EEG energy was also calculated at the frequency domain. As the Parseval's
theorem anticipates the energies were identical. The EEG energy was found concentrated at the four basic bands (α (8–13 Hz),
β(14–30 Hz), δ (0–4 Hz) and θ(5–7 Hz)). The primary concern of the present study was the gender related influence of EMF on
the spectral energy of EEG. The results show evidence of a strong gender—radiation interaction effect on the EEG energy and
on the peak amplitudes within each of the four rhythms. Without radiation the spectral power of males is greater than of females,
while under exposure the situation is reversed. Under the influence of EMF the spectral power of the males EEG is decreased
while that of the females is increased. In conclusion both the baseline EEG and the changes effected to the EEG power spectrum
under the influence of EMF seem to be gender dependent. 相似文献
7.
Regulation of microcirculation and other physiological processes have strong non-linear character and involves complex of
different processes, every process with own hierarchy in time and different frequencies. Traditional Fourier analysis does
not provide sufficient power and resolution to elucidate characteristic of low vasomotor frequencies. Therefore, we apply
a Time–frequency (wavelet) analysis on the signal obtained by Laser Doppler flow meter (LDF) at 25 healthy volunteers, exposed
at the same time to low frequency electromagnetic fields, used for physiotherapy. Signal processing include Matlab based algorithms
for digital signal processing (DSP) and Matlab Spectral analysis toolbox of simultaneous registered variations in Blood Pressure
(BP), Laser Doppler Flow (LDF), and Intravital microscopy (IVM). It provides useful information about regulatory mechanisms
and vegetative nervous system regulation of peripheral blood flow. Continuous changes in blood pressure variations and perfusion
of extremities were measured prior and after 10, 20, and 30 min ELF-EMF (10, 16, 20, and 30 mT), exposure. After wavelet analysis
of the blood flow signals and vasomotion changes signals, several frequency bands were distinguished: 0.0095–0.02 Hz; (α),
0.02–0.06 Hz; (β), 0.06–0.15 Hz; (γ), 0.15–0.4 Hz; (δ), and 0.4–1 Hz; (θ) for LDF data and 0.0095–0.4 Hz; (α), 0.4–0.75 Hz;
(β), 0.75–0.9 Hz; (γ), 0.9–1.2 Hz; (δ), and 1.2–2 Hz; (θ) for IVM data. In this study, overlapping of some frequency bands
between IVM and LDF data were found. Overlapping of the frequency bands has two ways of interpretation, one related with similarity
of the structures and tissues and other related with output of ELF-EMF stimulation. We used also correlation and cross-correlation
analysis to compare non-invasive (BP measurements and LDF) data, with invasive intravital microscopy (IVM) data (obtained
on animals in vivo), during ELF-EMF stimulation. IVM data were used as a reference value, for certain information of possible
mechanisms of biological response at the tissue and blood vessel level after ELF-EMF exposure with frequency in the range
from 10 to 50 Hz and magnetic flux density of 20 mT. Comparative analysis of IVM and LDF, frequency bands show that they have
statistical significant changes after ELF-EMF stimulation. Five subintervals were confirmed (α-, β-, γ-, δ-, and θ). The findings
indicate that local ELF-EMF exposure at the constant temperature of the media increases skin blood flow at the upper extremities
which have a contribution to the α-frequency band at IVM. 相似文献
8.
N. M. Salem 《The Environmentalist》2005,25(2-4):241-246
Summary The thermal effect due to electromagnetic absorption by human tissues is investigated at different frequencies ranging from
101–1016 Hz. Special emphasis is being considered in the microwave band used in wireless communications of the mobile industry. The
present work examines basically the thermal effect that arises as the duration of exposure to the electromagnetic radiation
and its dosage is increased. Using a computer simulation, a semi-classical model is adopted, were the photon-phonon interaction
is mediated by a homogenous sample of identical elements, which are naturally in harmonic modes of vibration. The phonon lifetime
is also included in this work. These thermal effects decrease with frequency in conformity with experimental results. This
model is then extrapolated to the individual cells of a human tissue and the possible rise in temperature is estimated. 相似文献
9.
The present study investigated the influence of electromagnetic fields (EMF), similar to that emitted by mobile phones, on
brain activity. Ten women and ten men, matched for age and educational level, performed a short memory task (Wechsler test),
with simultaneous Electroencephalogram (EEG) recordings at 15 scalp electrodes, both without (baseline) and with exposure
to an 1,800 MHz signal. The EEG energy was found concentrated at the four basic EEG bands [α (8–13 Hz), β (14–30 Hz), δ (0–4 Hz), and θ (5–7 Hz)]. The analysis revealed that in the presence of EMF, the energy of the β band was significantly greater for females than for males at the majority of the electrodes. Since beta oscillation is associated
with the shift of attention during the perception, these findings may indicate that the particular EMF (1,800 MHz signal)
exerts an influence on this brain activity, which appears to be gender-related. 相似文献
10.
Varsik Martirosyan Levon Markosyan Hrachik Hovhanesyan Karlen Hovnanyan Sinerik Ayrapetyan 《The Environmentalist》2012,32(2):157-165
The aim of this work was to investigate the frequency-dependent effects of extremely low-frequency electromagnetic field (ELF-EMF)
and mechanical vibration at infrasound frequency (MV at IS frequency or MV) on growth and development of Escherichia coli K-12, by using classical microbiological (counting colony forming units), isotopic, spectrophotometric and electronmicroscopic
methods. The frequency-dependent effects of MV and ELF-EMF were shown that they could either stimulate or inhibit the growth
and the division of microbes depending on the periods following exposure. However, the mechanism through which the MV and
ELF-EMF effects affect the bacteria cell is not clear yet. It was suggested that the aqua medium could serve a target through
which the biological effect of MV and ELF-EMF on microbes could be realized. To check this hypothesis, the frequency-dependent
effects (2, 4, 6, 8, 10 Hz) of both MV and ELF-EMF on the bacterial growth, division and their motility in cases of exposure,
the preliminary treated microbes-free medium and microbes containing medium were studied. Both MV and ELF-EMF effect on microbes
have frequency and post-exposure period duration-dependent characters. The [
3
H]-thymidine involving experiments shown that EMF at 4 Hz exposure has pronounced stimulation effect on cell proliferation while 4 Hz
MV has inhibition effect. But at 8–10 Hz, the both EMF and MV have inhibitory effects on cell proliferation. It is suggested
that 4 and 8 Hz EMF have different biological effects on microbes. 相似文献
11.
Hiie Hinrikus Ruth Tomson Jaanus Lass Deniss Karai Jaak Kalda Viiu Tuulik 《The Environmentalist》2005,25(2-4):157-163
Summary This study is aimed to investigate dependence of nerve pulse conduction velocity on low-level microwave exposure. The changes
in fiber membrane permeability or myelin sheath, caused by microwave, should affect nerve pulse conduction velocity. Experimental
investigation was carried out for nerve pulse conduction velocity with and without applied microwave field and different orientations
of its polarization to the nerve fiber. Microwave radiation of 450 MHz was applied polarized perpendicular or parallel to
the nerve fiber. The measured field power density at the skin was 0.87 mW/cm2. Special program was developed for calculation of the nerve pulse conduction velocity in LabView environment. The right nervus
medianus motor nerve fibers of 35 young subjects were under the study. The experimental protocol included ten measurements
in every cycle of five different exposure conditions. No changes in average values of the conduction velocities discovered
in different exposure conditions. Theoretical calculations based on Hodgkin-Huxley model confirm only a weak possible effect.
The results showed that low-level microwave radiation did not cause statistically significant change in nerve pulse conduction
velocity in human motor nerve fiber. 相似文献
12.
Martino Grandolfo 《The Environmentalist》2009,29(2):109-117
Over the last years, a wide debate has developed on the possible health effects of exposure to electromagnetic fields. In-depth
research activity was therefore developed by the international scientific community aimed at evaluating the risk associated
with exposure to this type of radiation. At the same time, various international institutions began to issue recommendations
on exposure limits valid for workers and for the population in the frequency range up to 300 GHz. Most of the recently revised
safety standards worldwide are set in terms of internal rates of electromagnetic energy deposition (Specific Absorption Rate)
at radiofrequency and microwave frequencies, and of induced electric fields or current densities at lower frequencies up to
10 MHz. At the international level, the most authoritative guidelines have been developed by the International Commission
on Non-Ionizing Radiation Protection (ICNIRP); another internationally well recognized standard is that developed by the Institute
of Electrical and Electronics Engineers (IEEE) in the USA, adopting the same basic approach of ICNIRP, although with some
differences in numerical values.
This article is mainly focused on the analysis of different approaches for the protection against electromagnetic fields,
and on the rationale of most relevant standards. 相似文献
13.
Mehri Kaviani Moghadam Saied Mohammad Firoozabadi Mahyar Janahmadi 《The Environmentalist》2008,28(4):341-347
In spite of growing concern about the influence of magnetic fields on biological systems, the interaction between extremely
low frequency magnetic field (ELF magnetic fields) and biological structures at the cellular level remains obscure. The aim
of this study was to investigate if 50 Hz magnetic fields could have an effect on the neuronal excitability and firing responses.
Under Current-Clamp condition, exposure to 50 Hz ELF magnetic fields at 2 mT or 0.8 mT intensities resulted in an increase
in the peak amplitude of action potential and after hyperpolaization potential in a time dependent manner. Both magnetic field
intensities decreased also the firing frequency and the duration of action potential. Taken together, these data suggest that
50 Hz ELF magnetic fields at 2 mT or 0.8 mT intensities may change the electrophysiological behavior of neuronal cells and
underlying ion channel currents. 相似文献
14.
Yngve Hamnerius 《The Environmentalist》2009,29(2):118-123
The rapid development of wireless technologies leads to increased human exposure to electromagnetic fields from new devices.
Most of these technologies communicate in short to medium range. Communication devices, such as mobile phones (GSM, UMTS,
LTE) and wireless computer networks (WLAN, HSDPA, WIMAX) usually work at distances up to some 10 km. Other techniques like
Bluetooth, RFID, and wireless USB work at distances up to a few meters. RFID systems can use several frequency bands from
low frequencies up to microwaves. The other technologies are mainly using microwave frequencies. Most of these technologies
have a rather low-output power, typically <1 W average power, except for fixed transmitters like base stations. This means
that the exposure from distant sources is low. If the devices are kept close to the body, the local exposure can be in the
range of the levels in the ICNIRP recommendation; this is the case, for example, for mobile phones and WLAN transmitters in
laptops. For distant sources, there exist several measurement techniques such as spectrum analysers, measurement receivers,
and broadband meters. For sources used close to the body, the local SAR levels have to be determined. For this purpose, instruments
measuring the local electric field inside body phantoms have been developed. An alternative to measurements is numerical simulations.
If one has knowledge of the signal characteristics of the different technologies then it is possible to find a suitable measurement
technique to assess the human exposure. 相似文献
15.
A. Wieraszko Z. Ahmed J. Armani N. Maqsood S. Philips H. Raja 《The Environmentalist》2005,25(2-4):145-155
Summary The influence of pulsed magnetic fields (PMF) on the properties of nervous tissue was investigated. Hippocampal slices or
synaptosomes obtained from hippocampal tissue were used as model systems. The amplitude of potentials recorded in vitro from one of the hippocampal pathways (Schaffer collaterals that use glutamate as a neurotransmitter) was employed as a measure
of the influence of magnetic fields on synaptic efficiency. The synaptic glutamate turnover and radioactive calcium accumulation
were used as markers of the PMF influence on biochemistry of synaptic mechanisms. The exposure of hippocampal slices for 30
min to PMF amplified evoked potentials. While the frequency of 0.16 Hz exerted the strongest effect, lower (0.01, 0.07, 0.03
Hz) and higher (0.5 Hz) frequencies were much less effective. The enhancement of the neuronal excitability was correlated
with significant increase in the neuronal spontaneous activity mediated by electrical synapses. The PMF-induced changes in
the excitability of the tissue were accompanied by an increase in the synaptic turnover of glutamate. The release of radioactive
D-Aspartate (a glutamate analog used as a marker for glutamate turnover) from the slices, and its uptake by synaptosomes were
enhanced, and reduced respectively, following the stimulation with pulsed magnetic fields. The frequency which was the most
efficient in amplification of evoked potentials (0.16 Hz) was also the most effective in the modulation of the release and
uptake processes. The PMF-induced changes in neurotransmitter turnover coincided with an increase in 45Ca2+ accumulation observed in hippocampal slices exposed to PMF. 相似文献
16.
Occupational noise exposure and noise-induced hearing loss (NIHL) has been recognised as a problem among workers in Indian
industries. The major industries in India are based on the processing of agricultural products. There are appreciable numbers
of pulse processing units spread throughout the country. The purpose of this study was to determine the prevalence of hearing
loss among pulse processing workers. As a part of hearing protection intervention, audiometric tests were conducted at the
binaural low (250–1,500 Hz), the binaural mid (1,500–4,000 Hz) and the binaural high (3,000–8,000 Hz) frequency averages.
The prevalence of hearing loss was determined based on hearing threshold levels (HTLs) with a low fence of 25 dB. Over 50%
of pulse processing workers (dana bazaar and dal mill) showed hearing loss in the noise-sensitive higher (binaural mid and
high) frequencies. The rate of hearing loss was particularly high among workers in the dal mill. The analyses show a higher
risk of prevalence of hearing loss among the dal mill workers compared to the workers associated with the grain preprocessing
activities. The study shows alarming signals of NIHL, especially in the dal mill workers. The occupational exposure to noise
could be minimised by efficient control measures through engineering controls, administrative controls and the use of personal
protective devices. Applications of engineering and/or administrative controls are frequently not feasible in developing countries
for technical and financial reasons. A complete hearing conservation programme, including training, audiometry and the use
of hearing protection devices, is the most feasible method for the protection of industrial workers from prevailing noise
in workplace environments in the developing countries. 相似文献
17.
Y. R. Mikayelyan N. S. Baghdasaryan A. K. Nikoghosyan S. V. Barseghyan S. N. Ayrapetyan 《The Environmentalist》2012,32(2):179-187
The effects of extremely low-frequency electromagnetic field (ELF EMF) and ELF-modulated millimeter waves (MMW) on physicochemical
properties of physiological solution (PS) at different environmental medium were studied. The existence of frequency “windows”
at 4 Hz and 8 Hz frequencies of ELF EMF and ELF-modulated MMW which have different effects on heat fusion period (HFP), hydrogen
peroxide (H2O2) formation and oxygen (O2) content of water solution and different dependency on temperature, background radiation (BGR) and illumination was shown.
The obtained data allow us to suggest that cell bathing medium serving as a target through which the biological effects of
ELF EMF and ELF-modulated MMW on cells are realized is extra sensitive to environmental factors. Therefore, the variability
of experimental data on EMF biological effects, obtained in different laboratories can be explained by different environmental
conditions of experiments, which very often are not considered adequately. 相似文献
18.
Henrietta Nittby Bengt Widegren Morten Krogh Gustav Grafström Henrik Berlin Gustav Rehn Jacob L. Eberhardt Lars Malmgren Bertil R. R. Persson Leif G. Salford 《The Environmentalist》2008,28(4):458-465
We have earlier shown that radio frequency electromagnetic fields can cause significant leakage of albumin through the blood–brain
barrier of exposed rats as compared to non-exposed rats, and also significant neuronal damage in rat brains several weeks
after a 2 h exposure to a mobile phone, at 915 MHz with a global system for mobile communications (GSM) frequency modulation,
at whole-body specific absorption rate values (SAR) of 200, 20, 2, and 0.2 mW/kg. We have now studied whether 6 h of exposure
to the radiation from a GSM mobile test phone at 1,800 MHz (at a whole-body SAR-value of 13 mW/kg, corresponding to a brain
SAR-value of 30 mW/kg) has an effect upon the gene expression pattern in rat brain cortex and hippocampus—areas where we have
observed albumin leakage from capillaries into neurons and neuronal damage. Microarray analysis of 31,099 rat genes, including
splicing variants, was performed in cortex and hippocampus of 8 Fischer 344 rats, 4 animals exposed to global system for mobile
communications electromagnetic fields for 6 h in an anechoic chamber, one rat at a time, and 4 controls kept as long in the
same anechoic chamber without exposure, also in this case one rat at a time. Gene ontology analysis (using the gene ontology
categories biological processes, molecular functions, and cell components) of the differentially expressed genes of the exposed
animals versus the control group revealed the following highly significant altered gene categories in both cortex and hippocampus:
extracellular region, signal transducer activity, intrinsic to membrane, and integral to membrane. The fact that most of these
categories are connected with membrane functions may have a relation to our earlier observation of albumin transport through
brain capillaries. 相似文献
19.
Antonietta Albanese E. Battisti G. Pompella A. Ghezzi A. Vittoria G. L. Messa A. L. Pasqui G. Campoccia L. Bianciardi N. Giordano G. Galassi 《The Environmentalist》2009,29(2):196-199
In our paper we studied the effects of exposure to ELF (extremely low frequency) or musically generated TAMMEF (therapeutic
application of musically modulated electromagnetic fields) electromagnetic fields on the tumor necrosis factor alpha (TNF-α)
release induced by a lipopolysaccharide (LPS) from cultured human lymphomonocytes of peripheral blood. Lymphomonocytes, isolated
from blood donors buffy-coat, were prepared using standard techniques in cell culture flasks, kept in CO2 incubator, with controlled temperature and humidity for 5 days. Flasks were subjected to an ELF electromagnetic field (100 Hz
sinusoidal) or to TAMMEF electromagnetic fields (with intensity, frequency, and wave shape randomly modified in time, so that
all possible codes can occur during a single application). The TNF-α release was determined by ELISA test every 24 h for 92 h
and the results were evaluated by a non-parametric test. LPS induces a stronger TNF-α release in cultures that were subjected
to ELF when compared with cultures subjected to TAMMEF, at each time period of the experimental protocol. These results seem
to indicate that TAMMEF is able to induce a complex modulation of LPS-induced TNF-α release, a cytokine with pro inflammatory
property whose release during chronic or neoplastic inflammatory diseases has strong negative effects on several organ systems. 相似文献
20.
Summary Biological effects of extremely low-frequency electromagnetic fields (ELF-EMF) on microcirculation were investigated in vivo by monitoring arteriole diameters in conscious mice. Measurements of blood vessel diameter were monitored 33 min non-stop
before during and after exposure with ELF-EMF and every 389 ms blood vessel diameter were calculated.
Using a dorsal skinfold chamber (DSC), and following caudal vein injection of FITC-dextran 250 kDa, the microvasculature (initial
arteriole diameter of 45–80 μm), was examined by intravital microscopy and video images were recorded for a total time of
33 min. Arteriole diameter was continuously measured by on-line analysis using a High-speed Digital Machine Vision System
CV-2100, using an edge-gap detection algorithm. Since vessel diameters exhibit rhythmic variation expressed by vasomotion,
for estimation of microcirculatory activity we used both raw data for frequency analysis of vasomotion (measured frequencies
of vasomotion were in the range 0.008÷0.1 Hz) and evaluate mean blood vessel diameter for each 1 min period of time, and make
a comparison between Pre, Exposure/Sham exposure and Post exposure periods, with the aim to evaluate possible changes in mean
blood vessel diameter as a result of ELF-EMF action.
During EMF exposure and post-exposure periods, arteriole diameters increased significantly compared with the pre-exposure
period, and the changes were larger during post-exposure. In contrast to sham exposure, vasodilatation of the microvasculature
was significantly greater during exposure and post-exposure to 16 Hz EMF. These findings suggest that ELF-EMF may have potential
therapeutic use benefit for treating vascular disorders. 相似文献