污染土壤修复效果评估方法研究综述

综述了评估污染土壤修复效果的指标及方法,包括残留污染分析法、风险评估法、植物毒性法、动物毒性法、微生物毒性法、土壤酶水平法等典型的评估方法,分析了各种方法的优缺点及适用范围。其中,植物毒性法适用于再利用类型为工业、商业用地的场地;动物毒性法适用于再利用类型为居住用地的场地;土壤微生物法、土壤酶水平法适用于再利用类型为农业用地的场地;对于存在人体暴露途径的用地,应结合人体健康风险评估法进行风险评估,综合评定土壤修复的效果。最后对该领域今后的研究方向进行了展望。     

石油污染土壤生物修复技术的研究进展

目前对石油污染环境的治理方法有物理法、化学法和生物修复三种类型。文章综合论述了国内外对石油污染土壤的几种微生物修复方法,分别介绍了利用植物、动物及微生物进行治理的修复技术。在我国,对于石油污染的治理应更多地采用低成本、无污染、高效率的生物治理技术。     

重金属污染土壤的修复方法及其在几类典型土壤修复中的应用

文中首先阐述了重金属污染土壤的途径与特点。介绍了当前几类主要的重金属污染土壤修复方法:物理化学方法、植物修复法、微生物修复法、动物修复法等的技术要点,详述了各自的技术特点及机理,并对它们的修复效能、环境友好性等进行了比较。从土壤的特性出发,区分出了3种典型的遭受重金属污染的土壤:农业土壤、城市土壤、矿区土壤,并从国内外最新的重金属污染修复实践中选取了适合各自土壤类型的修复方法进行了重金属污染土壤的治理探讨。最后,对土壤重金属污染修复技术的发展进行了展望。     

化学氧化技术对微生物修复石油污染土壤的强化作用研究

微生物修复技术由于其经济性和环境友好性在石油污染修复领域得到广泛应用，利用化学氧化技术可有效提高微生物修复效率。本研究以油泥污染土壤为研究对象，利用化学氧化—微生物联合技术开展修复研究，对比分析单一修复方法和联合修复法的修复效果。研究结果表明，利用化学氧化—微生物联合法去除石油烃的效率要优于化学氧化法和微生物法，最高去除率为48.82%，反应时间越长时，联合修复技术的优势越明显。研究结果可为联合修复技术在石油污染土壤治理方面的推广应用提供参考依据。     

石油污染土壤修复技术研究进展

研究高效、环保、低成本的石油污染土壤修复技术对石油开发场地修复具有重要的现实应用意义。从工艺和原理两个方面介绍和总结了焚烧法、洗涤法、气相抽提法、化学氧化法、微生物和植物法修复的研究现状及发展趋势。对上述工艺进行比较,总结出各类工艺的优缺点及使用范围。由于污染场地的地质结构、气候条件和污染程度等情况复杂,仅靠单一工艺很难达到预期目标,有必要对已有技术进行完善并开发组合土壤修复技术及设备,满足实际工程的需求。     

污染场地修复技术的种类

本文旨在归纳总结目前国内外对污染场地的修复的各种技术。认为场地修复通常包括污染土修复和含水层净化等2个方面。污染土常见的处理技术目前大致可归纳为6类，即微生物修复技术、化学处理技术、物理分离技术、固化／安定化技术、高温处理技术、植物修复技术等。而污染地下水的修复方法主要有5项：注气法、原位微生物修复技术、两相蒸气提取法、原位氧化法、原位反应墙技术等。     

土壤重金属污染及其修复技术研究

土壤重金属污染物有汞、镉、铅、锌等,主要来源于交通运输、工业污染和农业污染。土壤重金属污染会导致农作物减产甚至死亡,对人体健康也会产生极大危害。目前土壤重金属污染修复的技术主要包括工程修复法、物理化学修复法、化学修复法和生物修复法。植物修复技术作为一种新兴的绿色、生态、高效的修复技术具有良好的发展前景。     

电动及其联用技术在污染场地修复中的应用研究进展

近年来土壤污染日益严重，由无机污染物（重金属、放射性元素等）和有机污染物（多环芳烃等）污染的土壤已经对人类健康和生态环境构成风险，引起了广泛关注。电动修复技术（Electrokinetic remediation,EK）是一种可原位修复污染土壤的技术，由于其对污染场地修复的高效性，现已成为污染场地修复的研究热点。综述了电动修复技术的原理、优缺点，电动-植物、电动-微生物、电动-可渗透反应墙（Permeable reactive barrier,PRB）、电动-淋洗等电动联用技术修复污染场地土壤的研究进展，并总结了实际污染场地修复对土壤理化性质的影响，提出了该项技术的发展趋势和研究方向。     

初探污染场地的土壤修复工作过程与修复技术

"土壤修复"产业,我国目前处于起步阶段,其工作过程和技术路线是迫切需要解决的问题。本文在调研市场需求的基础上,探讨了土壤修复的工作流程、工作内容和修复技术。土壤修复的前期工作是场地土壤环境调查评估,首先从场地土壤环境质量(污染)调查开始,通过土壤样品采集、分析测试、结果分析和环境现状评价,进行场地土壤污染风险识别,包括污染因子、污染程度、修复因子的识别;通过污染风险评价,确定修复目标值、场地修复范围和修复量;通过试验筛选修复技术和修复工艺,确定修复方案和修复工程设计;对修复工程设计进行环境影响评价和环保行政审查;环保审查通过,实施修复工程。样品采集和分析测试、结果分析和评价、修复技术和修复工程成为"土壤修复"产业的工作核心。市场上应用的修复技术路线主要是隔离/封闭技术、稳定/固化技术、淋洗技术。     

污染场地修复技术效能比较

日益严重的环境污染,造成大片污染场地亟待进行修复。通过分析污染场地的主要污染物及来源,综述了应用于污染场地土壤修复的主要技术及优缺点;通过对国内外主要污染场地土壤修复技术特点及其效能的比较,探讨了我国污染场地修复技术的发展趋势。     

石油污染土壤微生物联合修复技术研究进展

石油是一种具有生物毒性的复杂有机物,土壤中石油烃的过量积累会给生态环境和人类健康造成严重威胁。微生物联合修复技术因处理成本低、环境影响小、无二次污染等优点,且较微生物修复或植物修复效率更高,耗时更短,成为当前的研究热点。文章介绍了植物-微生物联合修复、电动-微生物联合修复、氧化-微生物联合修复和表面活性剂强化微生物修复四种联合修复技术,简要阐述了修复机理、适用范围和工艺参数,为生物修复技术的选择提供了参考,并对以后生物修复的研究重点进行了展望。     

Microbial response to heavy metal-polluted soils: community analysis from phospholipid-linked fatty acids and ester-linked fatty acids extracts

Heavy metal pollution of soil is of concern for human health and ecosystem function. The soil microbial community should be a sensitive indicator of metal contamination effects on bioavailability and biogeochemical processes. Simple methods are needed to determine the degree of in situ pollution and effectiveness of remediating metal-contaminated soils. Currently, phospholipid-linked fatty acids (PLFAs) are preferred for microbial profiling but this method is time consuming, whereas direct soil extraction and transesterification of total ester-linked fatty acids (ELFAs) is attractive because of its simplicity. The 1998 mining acid-metal spill of >4000 ha in the Guadiamar watershed (southwestern Spain) provided a unique opportunity to study these two microbial lipid profiling methods. Replicated treatments were set up as nonpolluted, heavy metal polluted and reclaimed, and polluted soils. Inferences from whole community-diversity analysis and correlations of individual fatty acids with metals suggested Cu, Cd, and Zn were the most important in affecting microbial community structure, along with pH. The microbial stress marker, monounsaturated fatty acids, was significantly lower for reclaimed and polluted soil over nonpolluted soils for both PLFA and ELFA extraction. Another stress marker, the monounsaturated to saturated fatty acids ratio, only showed this for the PLFA. The general fungal marker (18:2omega6c), the arbuscule mycorrhizae marker (16:1omega5c), and iso- and anteiso-branched PLFAs (gram positive bacteria) were suppressed with increasing pollution whereas 17:0cy (gram negative bacteria) increased with metal pollution. For both extraction methods, richness and diversity were greater in nonpolluted soils and lowest in polluted soils. The ELFA method was sensitive for reflecting metal pollution on microbial communities and could be suitable for routine use in ecological monitoring and risk assessment programs because of its simplicity and reproducibility.     

土壤污染的健康危害与修复技术

土壤污染是当今社会面临的重要环境问题之一,已经对人体健康构成了严重威胁。本文将土壤污染物分为有机污染物、无机污染物、生物污染物和放射性污染物,并阐述了各类污染物对人体健康的危害,介绍了具有广阔应用前景的污染土壤修复技术,即植物修复技术和微生物修复技术。最后,本文还对解决土壤污染问题提出了具体的建议。     

结合《土壤污染防治行动计划》探讨中国土壤环境监管制度与标准值体系建设

2016年,中国国务院印发《土壤污染防治行动计划》(简称《土十条》),明确提出对农用地土壤实施分类管理,按照污染程度划分为优先保护类、安全利用类和严格管控类;对建设用地要防范新增污染,针对疑似污染地块,开展土壤环境状况调查评估,保障地块安全再利用;以影响农产品质量和人居环境安全的突出环境问题为重点,制定土壤污染治理与修复规划,组织开展治理与修复。实施严格的土壤环境质量保护、污染土壤的风险管控、高风险污染土壤的治理与修复是落实《土十条》的重要任务,也是建设我国土壤环境监管制度的重要内容。土壤环境标准是实施土壤环境管理的重要依据。本文基于《土十条》重要任务以及当前及今后一段时期国家土壤环境管理需求,以土壤环境管理需求为导向,探讨提出了包括土壤环境质量保护标准值、土壤环境风险管控标准值、污染土壤修复标准值的土壤环境标准值体系,可望为国家土壤环境标准体系的建立和标准制修订方法的发展提供技术参考。     

Impact of genetically modified crops on soil- and plant-associated microbial communities

Transgenic or genetically modified plants possess novel genes that impart beneficial characteristics such as herbicide resistance. One of the least understood areas in the environmental risk assessment of genetically modified crops is their impact on soil- and plant-associated microbial communities. The potential for interaction between transgenic plants and plant residues and the soil microbial community is not well understood. The recognition that these interactions could change microbial biodiversity and affect ecosystem functioning has initiated a limited number of studies in the area. At this time, studies have shown the possibility that transgenes can be transferred to native soil microorganisms through horizontal gene transfer, although there is not evidence of this occurring in the soil. Furthermore, novel proteins have been shown to be released from transgenic plants into the soil ecosystem, and their presence can influence the biodiversity of the microbial community by selectively stimulating the growth of organisms that can use them. Microbial diversity can be altered when associated with transgenic plants; however, these effects are both variable and transient. Soil- and plant-associated microbial communities are influenced not only by plant species and transgene insertion but also by environmental factors such as field site and sampling date. Minor alterations in the diversity of the microbial community could affect soil health and ecosystem functioning, and therefore, the impact that plant variety may have on the dynamics of the rhizosphere microbial populations and in turn plant growth and health and ecosystem sustainability, requires further study.     

A modified risk assessment to establish molybdenum standards for land application of biosolids

The USEPA standards (40 CFR Part 503) for the use or disposal of sewage sludge (biosolids) derived risk-based numerical values for Mo for the biosolids --> land --> plant --> animal pathway (Pathway 6). Following legal challenge, most Mo numerical standards were withdrawn, pending additional field-generated data using modern biosolids (Mo concentrations <75 mg kg(-1) and a reassessment of this pathway. This paper presents a reevaluation of biosolids Mo data, refinement of the risk assessment algorithms, and a reassessment of Mo-induced hypocuprosis from land application of biosolids. Forage Mo uptake coefficients (UC) are derived from field studies, many of which used modern biosolids applied to numerous soil types, with varying soil pH values, and supporting various crops. Typical cattle diet scenarios are used to calculate a diet-weighted UC value that realistically represents forage Mo exposure to cattle. Recent biosolids use data are employed to estimate the fraction of animal forage (FC) likely to be affected by biosolids applications nationally. Field data are used to estimate long-term Mo leaching and a leaching correction factor (LC) is used to adjust cumulative biosolids application limits. The modified UC and new FC and LC factors are used in a new algorithm to calculate biosolids Mo Pathway 6 risk. The resulting numerical standards for Mo are cumulative limit (RPc)=40 kg Mo ha(-1), and alternate pollutant limit (APL) = 40 mg Mo kg(-1) We regard the modifications to algorithms and parameters and calculations as conservative, and believe that the risk of Mo-induced hypocuprosis from biosolids Mo is small. Providing adequate Cu mineral supplements, standard procedure in proper herd management, would augment the conservatism of the new risk assessment.     

Phosphorus restrictions for land application of biosolids: current status and future trends

The application of biosolids (sewage sludge) to agricultural soils provides P in excess of crop needs when applied to meet the N needs of most agronomic crops. These overapplications can result in the buildup of P in soils to values well above those needed for optimum crop yields and also may increase risk of P losses to surface and ground waters. Because of concerns regarding the influence of P on water quality in the USA, many state and federal agencies now recommend or require P-based nutrient management plans for animal manures. Similar actions are now under consideration for the land application of biosolids. We reviewed the literature on this subject and conducted a national survey to determine if states had restrictions on P levels in biosolids-amended soils. The literature review indicates that while the current N-based approach to biosolids management does result in increases of soil P, some properties of biosolids may mitigate the environmental risk to water quality associated with land application of P in biosolids. Results of the survey showed that 24 states have regulations or guidelines that can be imposed to restrict land application of biosolids based on P. Many of these states use numerical thresholds for P in biosolids-amended soils that are based on soil test phosphorus (STP) values that are much greater than the values considered to be agronomically beneficial. We suggest there is the need for a comprehensive environmental risk assessment of biosolids P. If risk assessment suggests the need for regulation of biosolids application, we suggest regulations be based on the P Site Index (PSI), which is the method being used by most states for animal manure management.     

In situ bioremediation through mulching of soil polluted by a copper-nickel smelter

Bioremediation of a heavy metal-polluted soil was investigated in a 3-yr field experiment by adding mulch to a polluted forest floor. The mulch consisted of a mixture of compost and woodchips. The remediation treatment decreased the toxicity of the soil solution to bacteria as determined by the [3H]-thymidine incorporation technique, that is, by measuring the growth rate of soil bacteria extracted from unpolluted humus after exposing them to soil solution containing heavy metals from the experimental plots. Canonical correlation analysis was performed in order to identify the chemical and microbiological changes in the soil. The pH of the mulched organic layer increased by one unit. The concentration of complexed Cu increased and that of free Cu2+ decreased in the soil solution from the mulch treatment. According to basal respiration and litter decomposition, microbial activity increased during the 3 yr following the remediation treatment. The [3H]-thymidine incorporation technique was also used to study the growth rate and tolerance of bacteria to Cu. The bacterial growth rate increased and the Cu tolerance decreased on the treated plots. The structure of the microbial community, as determined by phospholipid fatty acid (PLFA) analysis, remained unchanged. The results indicate that remediation of the polluted soil had occurred, and that adding a mulch to the forest floor is a suitable method for remediating heavy metal-polluted soil.     

Changes in Labile Organic Carbon Fractions and Soil Enzyme Activities after Marshland Reclamation and Restoration in the Sanjiang Plain in Northeast China

The extensive reclamation of marshland into cropland has tremendously impacted the ecological environment of the Sanjiang Plain in northeast China. To understand the impacts of marshland reclamation and restoration on soil properties, we investigated the labile organic carbon fractions and the soil enzyme activities in an undisturbed marshland, a cultivated marshland and three marshlands that had been restored for 3, 6 and 12?years. Soil samples collected from the different management systems at a depth of 0-20?cm in July 2009 were analyzed for soil organic carbon (SOC), dissolved organic carbon (DOC), microbial biomass carbon (MBC) and easily degradable organic carbon. In addition, the activities of the invertase, β-glucosidase, urease and acid phosphatase were determined. These enzymes are involved in C, N and P cycling, respectively. Long-term cultivation resulted in decreased SOC, DOC, MBC, microbial quotient and C (invertase, β-glucosidase) and N-transforming (urease) enzyme activities compared with undisturbed marshland. After marshland restoration, the MBC and DOC concentrations and the soil invertase, β-glucosidase and urease activities increased. Soil DOC and MBC concentrations are probably the main factors responsible for the different invertase, β-glucosidase and urease activities. In addition, marshland restoration caused a significant increase in the microbial quotient, which reflects enhanced efficiency of organic substrate use by microbial biomass. Our observations demonstrated that soil quality recovered following marshland restoration. DOC, MBC and invertase, β-glucosidase and urease activities were sensitive for discriminating soil ecosystems under the different types of land use. Thus, these parameters should be considered to be indicators for detecting changes in soil quality and environmental impacts in marshlands.     

Improvement of soil quality after "alperujo" compost application to two contaminated soils characterised by differing heavy metal solubility

Reclamation of trace element polluted soils often requires the improvement of the soil quality by using appropriate organic amendments. Low quality compost from municipal solid waste has been tested for reclamation of soils, but these materials can provide high amounts of heavy metals. Therefore, a high-quality compost, with low levels of heavy metals, produced from the main by-product of the Spanish olive oil extraction industry ("alperujo") was evaluated for remediation of soils affected by a pyritic mine sludge. Two contaminated soils were selected from the same area: they were characterised by differing pH values (4.6 and 7.3) and total metal concentrations, which greatly affected the fractionation of the metals. Compost was applied to soil at two rates (equivalent to 48 and 72 Tm ha(-1)) and compared with an inorganic fertiliser treatment. Compost acted as an available nutrient source (C, N and P) and showed a low mineralisation rate, suggesting a slow release of nutrients and thus favouring long term soil fertility. In addition, the liming effect of the compost led to a significant reduction of toxicity for soil microorganisms in the acidic soil and immobilisation of soil heavy metals (especially Mn and Zn), resulting in a clear increase in both soil microbial biomass and nitrification. Such positive effects were clearly greater than those provoked by the mineral fertiliser even at the lowest compost application rate, which indicates that this type of compost can be very useful for bioremediation programmes (reclamation and revegetation of polluted soils) based on phytostabilisation strategies.