磺酰脲类除草剂的残留分析

磺酰脲类除草剂是一类新型超高效除草剂，大约包括２Ｖ化合物，已被广泛用于禾谷作物防除阔叶杂草以及某些禾本科杂草，该类除草剂在环境中的残留浓度〈１μｇ．ｋｇ＾－１水平。常规的分析方法包括生物鉴定，酶免疫分析，气相色谱法和高效液相色谱法（ＨＰＬＣ）、ＨＰＬＣ／ＭＳ联用后，可以准确测定复杂环境样中磺酰脲类除草剂的痕量残留。     

磺酰脲类除草剂在硅胶G表面的光解

应用高效薄层析方法研究了苄嘧磺隆，甲磺隆，氯磺隆，氯嘧磺隆4种磺酰脲类除草剂在氙灯与太阳光下的光解动力学，以及8种有机色素对苄嘧磺隆光降解的影响。结果表明4种除草剂在硅胶G表面的降解呈一级动力学反应；有机色素对除草剂的降解均表现显著的猝灭效应。其中以姜黄素的猝灭作用最强，色素剂量与光解中的猝灭效应存在着显著的相关性。色素剂量加大，猝灭效应加强。     

Best Management Practices for Silvicultural Chemicals and the Science Behind Them

Silvicultural chemicals include fertilizers and pesticides applied for forest management. All states east of the Rockies have at least some form of silvicultural chemical best management practices (SCBMPs) and it is widely accepted that SCBMPs effect some protection of water quality. All SCBMPs recommend handling and application precautions and a minimum width streamside management zone (SMZ) on each side of streams for protection of water quality and aquatic ecosystems. Typically these zones increase in width as stream width increases. In areas where cold-water fish are present, additional widths are designated for their protection. Minimum SMZ widths range from 8 to 91 meters on each side of the stream bank for intermittent and perennial streams. Most SMZ recommendations do not cover ephemeral drainages, ditches or canals. SMZs are generally described as being site specific with width dependent on slope, soil type and other conditions as well as the stream's designation as perennial or intermittent. The science behind SCBMPs is often elusive. Spray drift can be controlled through proper selection and use of application technology while considering site specific conditions. SMZs greatly reduce the amount of herbicides reaching streams. Available toxicological data and research on aquatic ecosystem impacts from herbicide use suggest that additional protection from silvicultural chemicals may not be necessary. However, there is evidence to suggest that protection of ephemeral and intermittent channels can further reduce entry of silvicultural chemicals into streams and also reduce sedimentation. This may be the most fruitful area of research we can pursue in light of the knowledge gaps listed.     

Simultaneous determination of three herbicides by differential pulse voltammetry and chemometrics

A novel differential pulse voltammetry method (DPV) was researched and developed for the simultaneous determination of Pendimethalin, Dinoseb and sodium 5-nitroguaiacolate (5NG) with the aid of chemometrics. The voltammograms of these three compounds overlapped significantly, and to facilitate the simultaneous determination of the three analytes, chemometrics methods were applied. These included classical least squares (CLS), principal component regression (PCR), partial least squares (PLS) and radial basis function-artificial neural networks (RBF-ANN). A separately prepared verification data set was used to confirm the calibrations, which were built from the original and first derivative data matrices of the voltammograms. On the basis relative prediction errors and recoveries of the analytes, the RBF-ANN and the DPLS (D – first derivative spectra) models performed best and are particularly recommended for application. The DPLS calibration model was applied satisfactorily for the prediction of the three analytes from market vegetables and lake water samples.     

CONCENTRATIONS OF POLLUTANTS IN AGRICULTURAL RUNOFF1

ABSTRACT. .Eighteen rural lakes in Lubbock County were sampled on a routine basis following runoff-producing rainfall for a period of approximately eighteen months to determine whether or not runoff from intensively farmed agricultural areas contained significant concentrations of nitrates, phosphates, herbicides, or insecticides. An additional fifteen lakes lying within a triangle bounded by the cities of Plainview, Canyon, and Hereford, Texas, were sampled one time during the summer of 1969 to provide additional data regarding the nature and extent of the potential problem in an area with a different soil type and a slightly different cropping pattern. Based on results of detailed analyses of approximately two hundred samples of water collected from the lakes and an equal number of sediment samples collected from the same lakes at the same time, it appears that the concentrations of all chemical pollutants in runoff from agricultural lands in the High Plains are well below the allowable concentrations for drinking water.     

Postemergence Control of Microstegium vimineum on Riparian Restoration Sites with Aquatic‐Use Registered Herbicides

Microstegium vimineum is an invasive grass introduced from Asia that has spread throughout riparian areas of the eastern United States threatening native riparian vegetation. Postemergence (POST) herbicides registered for aquatic use were evaluated for control of M. vimineum on two riparian restoration sites in the Piedmont and Upper Coastal Plain of North Carolina. This study found that standard and lower than standard rates of diquat, fluridone, flumioxazin, glyphosate, imazamox, and imazapyr reduced weed stem density and biomass at 6 and 30 weeks after treatment (WAT). Both rates of bispyribac and penoxsulam provided less control of M. vimineum. Visual ratings showed both rates of diquat, flumioxazin, imazamox, and imazapyr controlled 63‐100% of M. vimineum at 6 WAT and 84‐100% at 30 WAT. Fluridone and glyphosate provided slightly less control. Bispyribac and penoxsulam treatments provided less control at 6 and 30 WAT compared to the other treatments. Plots treated with both rates of diquat, flumioxazin, imazamox, and imazapyr were nearly devoid of all vegetation at 30 WAT. Recommendations include POST application of lower than standard rates of diquat, flumioxazin, fluridone, glyphosate, imazamox, and imazapyr on riparian restoration sites infested with M. vimineum. Immediate vegetation management measures including temporary and permanent plant cover should be employed on treated sites where weeds are completely eradicated to prevent erosion.     

Leaching of mecoprop and dichlorprop in calcareous soil. Effect of the exogen organic matter addition in this process

Abstract

Leaching studies of mecorprop (R,S)‐2‐(4‐chloro‐2‐methylphenoxy)propanoic acid, and dichlorprop, (R,S)‐2‐(4‐chloro‐2,4‐dichlorophenoxy) propanoic acid, under saturated conditions were conducted in unamended and amended soil columns. The purpose of the study was to investigate the leaching of these herbicides in three type of soils and the exogen organic matter effect on this process. The leaching patterns could be related to variation in the soil texture and diffusion processes of the herbicides into micropores within the walls of conducting pore. The leaching rate in the amended soil columns decreased with the addition of organic matter. The breakthrough curves (BTC) of these herbicides in the leachates of the amended soil columns were wider and more diffused than the BTC obtained for the corresponding unamended soil. The theoretical BTC overestimated the pore volume required for the displacement of these pesticides from the soil column. This may be due to the differences in the adsorption process between the bacth and soil columns methods     

Effects of gene flow from IMI resistant sunflower crop to wild Helianthus annuus populations

Wild sunflower, H. annuus ssp. annuus, is an invasive species widely distributed in several regions of the world, including central Argentina where it shares a large area with domesticated sunflower. The discovery of imidazolinone resistance in a wild sunflower population allowed the development of Clearfield^® technology in cultivated sunflower. This technology was rapidly adopted in Argentina but the trait could possibly be transferred to the naturalized wild populations through natural hybridization. The aim of this study was to evaluate the transfer of IMI resistance to wild sunflower populations and its effect on wild plants’ fitness. Plants of five wild populations and their progenies of crosses with an IMI-resistant hybrid were evaluated through a dormancy, herbicide resistance, and SSR markers study. Relative fitness was compared in the five populations, F1s and backcrosses with wild and crop parental plants. Hybridization with an IMI-resistant hybrid did not alter seed dormancy. F1 individuals were more resistant to imazapyr than their wild ancestors but less tolerant than the commercial variety. SSR markers confirmed the transfer of resistance and identified resistant plants within the wild populations. Fitness was reduced in the first generation after crossing but was recovered in the following generations. Thus, to ensure durability and efficiency of Clearfield^® technology, management practices like crop rotation and herbicide usage with different modes of action should be considered.     

Three Different Topics for Bioremediation Application on Soils and Waters Contaminated with Aromatic Compounds, by Using Immobilized Bacteria

Molecular techniques and modelling are presented as powerful tools required in the performance of efficient soil and water bioremediation systems. An Escherichia coli CC118-D strain was constructed by inserting the Klebsiella pneumoniae hpa B gene, coding for the unstable 3,4-dihydroxyphenylacetate 2,3-dioxygenase, into its chromosome. When the constructed strain was immobilized, both enzyme stability and viability increased along the studied period, in absence of antibiotic. We proposed this strategy as an approach to overcoming plasmid instability and to enhance enzyme activity and stability, avoiding antibiotic utilization. A model was developed to understand and predict the behaviour of bacteria and pollutants in a bioreactor system, considering: fluid dynamics, molecular/cellular scale processes and biofilm formation.     

RELATIONSHIPS BETWEEN LAND USES AND RAINWATER QUALITY IN A SOUTHCENTRAL PENNSYLVANIA WATERSHED1

ABSTRACT: Spatial and temporal variability in rainfall concentrations of nutrients, major ions, and herbicides was monitored at 7 locations in or near the Conodoguinet Creek watershed in south-central Pennsylvania from 1991.1993. Results were used to (1) compare precipitation quality in forested, agricultural and urban areas, and (2) assess the practicality of using volunteer citizen monitoring in such a study. As indicated in previous studies, sulfate and nitrogen concentrations in precipitation were linked to sample pH. Concentrations of major ions in precipitation appeared to relate more to regional influences rather than local influences. However, concentrations of herbicides in precipitation may have been influenced by both regional and local use which caused compounds like atrazine, deethylatrazine, propazine, simazine, metolachior, alachlor, ametryn, and prometon to be present in detectable concentrations in rainfall. Seasonality was evident in nitrogen, sulfate, pH, and herbicide data and was suggested in calcium, iron, manganese, magnesium, orthophosphate, and chloride data. Agricultural weed control activities were probably responsible for the seasonal pattern in pesticide data which peaked in May and June. Tropical storm Danielle may have caused the apparent seasonal patterns for the other nine parameters. This storm did not follow the typical west to east movement pattern and consequently produced rainfall of relative high quality. A variety of quality assurance checks indicated that trained volunteer citizen monitors were successful participants in this intensive and extensive scientific study, collecting good quality samples in a timely manner. Without this kind of volunteer help, it is extremely difficult to complete studies that require sampling in response to natural events such as rainfall.