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使用不同分散剂制备了聚乙烯吡咯烷酮-硫化镉(PVP-CdS)催化剂,研究了盐酸黄连素在PVP-CdS修饰玻碳电极上的电化学行为。实验结果表明:与裸电极相比,PVP-CdS修饰玻碳电极提高了盐酸黄连素的氧化电流;当外加电压为0.5~1.4 V、电位增量为0.018 V、缓冲溶液pH为6时,盐酸黄连素质量浓度在1~200 mg/L的范围内与氧化峰电流呈良好的线性关系,检出限为0.15 mg/L。通过盐酸黄连素氧化前后的红外谱图分析,推断出了氧化机理。 相似文献
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The present investigation determined the effects of epibrassinolide (EBL) on the levels of indole-3-acetic acid (IAA), abscisic acid (ABA), and polyamine (PA) and antioxidant potential of 7-d old Raphanus sativus L. cv. ‘Pusa chetki’ seedlings grown under Cr (VI) metal stress. Reduced titers of free (0.767 μg g−1 FW) and bound (0.545 μg g−1 FW) IAA in Cr (VI) stressed seedlings were observed over untreated control. Supplementations of EBL to Cr (VI) stressed seedlings were able to enhance both free (2.14-5.68 μg g−1 FW) and bound IAA (2.45-7.78 μg g−1 FW) concentrations in comparison to Cr (VI) metal treatment alone. Significant rise in free (13.49 μg g−1 FW) and bound (12.17 μg g−1 FW) ABA contents were noticed for Cr (VI) stressed seedlings when compared to untreated control. No significant increase in ABA contents were recorded for Cr (VI) stressed seedlings upon supplementation with EBL over Cr (VI) treatment alone. A significant increase in Put (18.40 μg g−1 FW) and Cad (9.08 μg g−1 FW) contents were found for 10−9 M EBL plus Cr (VI) metal treatments when compared to Cr (VI) treatment alone. Spermidine (Spd) contents were found to decline significantly for EBL treatment alone or when supplemented with Cr (VI) treatments over untreated controls and Cr (VI) treatment alone. Antioxidant levels were found to enhance, with glutathione (57.98 mg g−1 FW), proline (4.97 mg g−1 FW), glycinebetaine (39.01 μmol mL−1), ascorbic acid (3.17 mg g−1 FW) and phytochelatins (65.69 μmol g−1 FW) contents noted for EBL supplemented to Cr (VI) metal solution over Cr (VI) treatment alone. Reduced activities of guaiacol peroxidase (0.391 U mg−1 protein) and catalase (0.221 U mg−1 protein) and enhanced activities of glutathione reductase (7.14 U mg−1 protein), superoxide dismutase (15.20 U mg−1 protein) and ascorbate peroxidase (4.31 U mg−1 protein) were observed in seedlings treated with EBL plus Cr (VI) over Cr metal treatment alone. Reduced MDA (2.55 μmol g−1 FW) and H2O2 (33.24 μmol g−1 FW) contents were recorded for 10−9 M EBL supplemented to Cr (VI) stress over Cr (VI) treatment alone. Enhancement in free radical scavenging potential as indicated by higher values of 1,1-diphenylpicrylhydrazyl, deoxyribose and reducing power activity assays, and increased levels of phenols and soluble sugars also showed significant influence of EBL in alleviating Cr (VI) stress in radish seedlings. 相似文献
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