Transformation of a non-oestrogenic steroid metabolite to an oestrogenically active substance by minimal bacterial activity

The majority of oestrogenic material excreted from humans and wildlife, and therefore released into sewers, is in a conjugated form. However, the finding of “free” oestrogens in sewage effluent suggests that these metabolites are somehow converted back into an active form, before or during passage through a sewage treatment process. When male fathead minnows (Pimephales promelas) were continuously exposed to oestradiol-3-glucuronide, in a continuous-flow system, it demonstrated no inherent oestrogenic activity. However, when fish were exposed to effluent generated from laboratory simulations of sewage treatment processes, to which had been added oestradiol-3-glucuronide, oestrogenic activity was observed, suggesting microbial activity was capable of degrading the steroid metabolite into a more potent oestrogen. Oestrogenic potency was determined by measuring changes in plasma vitellogenin (egg yolk precursor) concentrations and gonadosomatic index. The results suggest that inactive metabolites of steroids are very readily biotransformed into biologically active oestrogens.     

Correlation of ambient inhalable bioaerosols with particulate matter and ozone: a two-year study

In this study, we have examined the relationships between the concentrations of ambient inhalable airborne fungi and pollen with PM10, PM2.5, ozone, organic carbon, selected trace metals (cadmium, copper, lead, and zinc), temperature, and relative humidity. The database was collected in Cincinnati, Ohio, USA, during two consecutive years. Measurements of all environmental variables were performed at the same site continuously 5 days a week except during winter months. The airborne concentrations of biological and non-biological pollutants ranged as follows: total fungi: 184-16 979 spores m(-3); total pollen: 0-6692 pollen m(-3); PM10: 6.70-65.38 microg m(-3); PM2.5: 5.04-45.02 microg m(-3); and ozone: 2.54-64.17 ppb. Higher levels of total inhalable fungi and particulate matter were found during fall and summer months. In contrast, total pollen concentration showed elevated levels in spring. Peak concentrations of ozone were observed during summer and beginning of fall. Our study concluded that several types of inhalable airborne fungi and pollen, particulate matter, and ozone could be positively correlated as a result of the atmospheric temperature influence.     

Stabilising secondary materials markets

This paper argues that ‘secondary materials’ — waste products — are now an integral part of the global commodities problem. It analyses the background to attempts being made in several advanced economies to intervene in secondary materials markets. The forms of intervention are exact analogues of those that have been tried, or are being considered, and centre on buffer-stock schemes which aim to stabilise prices. In turn, stabilised markets have been thought to be a prerequisite for increasing the long-run supply of secondary materials. The paper casts doubt on the usefulness of such schemes for a selected product — waste paper — and presents a simulation exercise for the existence of a buffer-stock scheme.     

Metals recycling : A comparative national analysis

The purpose of this paper is to establish a definitive measure for a recycling rate in various countries. Numerous measures have been used in the past and have been based on a variety of assumptions, so that the literature has become very difficult to interpret. The objective here is to assess and explain how a recycling rate has been established such that it is unambiguous and serves as a reference point for future work. It then becomes possible to compare and contrast the recycling effort in different countries, and determine the potential for increased activity.     

Small-scale temporal variation and the effect of urbanisation on extracellular enzyme activity in streams

The activity of six extracellular enzymes involved in the degradation of dissolved organic carbon compounds was measured in two highly urbanised and two minimally impacted streams east of Melbourne, Australia, using 4-methylumbelliferyl-substrates. Small-scale temporal variation in enzyme activity was determined by repeatedly sampling the same point in the water column, while the effect of flow was determined by sampling in regions of higher and lower flow in both stream types. Replicate samples showed that enzyme activity was not significantly different over small (minutes) time scales. On five of six sampling occasions the enzyme activity was unaffected by flow. On one sampling occasion in a minimally disturbed stream, the difference between the high- and low-flow regions was statistically significant (ANOSIM, Global R= 0.78, P= 0.03). Enzyme activity profiles (activities of the suite of enzymes) of the streams in urbanised catchments were different to those in minimally disturbed catchments. The measurements made in four different streams showed high reproducibility over short time periods (minutes) which lends greater credibility to analogous spatial studies. Although these results determined that small-scale temporal variability was not significant, and that the effects of flow were generally minimal, it is recommended that spatial and temporal variability in the stream be at least considered before any studies measuring extracellular enzyme activity in stream waters are carried out. Such an approach will lead to conclusions from measurements that are not likely to be confounded by variables such as flow rate or time.     

Sampling over time: developing a cost effective and precise exposure assessment program

Studies requiring ambient exposure assessments invariably ask: How often should measurements be taken? Answer to such questions is dictated by budgetary considerations as well as spatial and temporal variability in the data. For example, do we obtain measurements during all seasons, all months within seasons, weeks within months and days within weeks? On one hand, we can obtain a one-time snapshot sample and regard it as representing the "true" mean exposure. On the other hand, we may obtain a large number of measurements over time and then average these in order to represent this "true" mean exposure. The former estimate is the least expensive but may also be the least precise while the latter, may be very precise but prohibitively costly. In this paper, we demonstrate how a pilot study can be undertaken with a potentially promising and feasible sampling plan for the full-scale study. By applying the statistical methodology of variance component analysis (VCA) to the pilot study data and exploiting mathematical relationship between the variance of the overall mean exposure and posited variance components, we can develop a sampling design with decreased sampling costs and/or increased precision of the mean exposure. Our approach was applied to determine sampling design choices for an on-going study that aimed at assessing ambient particulate matter exposure. We conclude that a pilot study followed by the VCA analysis may often lead to sampling design choices that offer considerable cost savings and, at the same time, promise to provide relatively precise estimates of the mean exposure for the subsequent full-scale study.     

Paper recycling and social policy

This paper examines the problems and operation of waste paper recycling in the UK. Long- and short-term forecasts for demand are developed, showing demand strongly correlated with Gross Domestic Product. These are used to simulate a buffer stock scheme and establish costs for operating such a scheme. The authors conclude, given that the only large source of waste paper not fully exploited is the householder, then grants for developing waste upgrading technologies are likely to be more effective than buffer stock schemes.     

A synthesis of convergent reflections,tensions and silences in linking gender and global environmental change research

This synthesis article joins the authors of the special issue “Gender perspectives in resilience, vulnerability and adaptation to global environmental change” in a common reflective dialogue about the main contributions of their papers. In sum, here we reflect on links between gender and feminist approaches to research in adaptation and resilience in global environmental change (GEC). The main theoretical contributions of this special issue are threefold: emphasizing the relevance of power relations in feminist political ecology, bringing the livelihood and intersectionality approaches into GEC, and linking resilience theories and critical feminist research. Empirical insights on key debates in GEC studies are also highlighted from the nine cases analysed, from Europe, the Americas, Asia, Africa and the Pacific. Further, the special issue also contributes to broaden the gender approach in adaptation to GEC by incorporating research sites in the Global North alongside sites from the Global South. This paper examines and compares the main approaches adopted (e.g. qualitative or mixed methods) and the methodological challenges that derive from intersectional perspectives. Finally, key messages for policy agendas and further research are drawn from the common reflection.