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51.
Enrico Cappellini M. Thomas P. Gilbert Filippo Geuna Girolamo Fiorentino Allan Hall Jane Thomas-Oates Peter D. Ashton David A. Ashford Paul Arthur Paula F. Campos Johan Kool Eske Willerslev Matthew J. Collins 《Die Naturwissenschaften》2010,97(2):205-217
We report here the first integrated investigation of both ancient DNA and proteins in archaeobotanical samples: medieval grape (Vitis vinifera L.) seeds, preserved by anoxic waterlogging, from an early medieval (seventh–eighth century A.D.) Byzantine rural settlement in the Salento area (Lecce, Italy) and a late (fourteenth–fifteenth century A.D.) medieval site in York (England). Pyrolysis gas chromatography mass spectrometry documented good carbohydrate preservation, whilst amino acid analysis revealed approximately 90% loss of the original protein content. In the York sample, mass spectrometry-based sequencing identified several degraded ancient peptides. Nuclear microsatellite locus (VVS2, VVMD5, VVMD7, ZAG62 and ZAG79) analysis permitted a tentative comparison of the genetic profiles of both the ancient samples with the modern varieties. The ability to recover microsatellite DNA has potential to improve biomolecular analysis on ancient grape seeds from archaeological contexts. Although the investigation of five microsatellite loci cannot assign the ancient samples to any geographic region or modern cultivar, the results allow speculation that the material from York was not grown locally, whilst the remains from Supersano could represent a trace of contacts with the eastern Mediterranean. 相似文献
52.
Local Agenda 21 (LA21) has emerged as the principal means of addressing sustainable development practice at the local government level. In the UK, progressive local authorities have emphasised the need for participatory processes and innovative policy options. This requires commitment and active involvement from a variety of individuals and organisations. Participants in LA21 have been interviewed to determine their motivations and perceptions, and their responses are represented in terms of the storylines of various constituencies of interest. The key themes seem to be those of actively promoting widespread participation, gaining competence in innovative techniques, taking a holistic approach to quality of life concerns, and claiming the legitimacy of local government as a key player in sustainable development. 相似文献
53.
Dorte Bekkevold Jane Frydenberg Jacobus J. Boomsma 《Behavioral ecology and sociobiology》1999,46(2):103-109
Queen mating frequency of the facultatively polygynous ant Acromyrmex echinatior was investigated by analysing genetic variation at an (AG)n repeat microsatellite locus in workers and sexuals of 20 colonies from a single Panamanian population. Thirteen colonies
were found to be monogynous, 5 colonies contained multiple queens, whereas the queen number of 2 colonies remained unresolved.
Microsatellite genotypes indicated that 12 out of 13 queens were inseminated by multiple males (polyandry). The mean queen
mating frequency was 2.53 and the mean genetically effective paternity frequency was 2.23. These values range among the highest
found in ants, and the results are in keeping with the high mating frequencies reported for other species of leafcutter ants.
Consistent skew in the proportional representation of different patrilines within colonies was found, and this remained constant
in two consecutive samples of offspring. Dissections showed that all examined queens from multiple-queen colonies were mated
egg-layers. The mean relatedness value among nestmate workers in polygynous colonies was lower than that for monogynous colonies.
No diploid males were detected in a sample of 70 genotyped males. Worker production of males was detected in one queenless
colony. We discuss our findings in relation to known patterns of multiple maternity and paternity in other eusocial Hymenoptera.
Received: 2 September 1998 / Received in revised form: 3 February 1999 / Accepted: 7 February 1999 相似文献
54.
A curious feature of the honeybee's waggle dance is the imprecision in the direction indication for nearby food sources.
One hypothesis for the function of this imprecision is that it serves to spread recruits over a certain area and thus is an
adaptation to the typical spatial configuration of the bees' food sources, i.e., flowers in sizable patches. We report an
experiment that tests this tuned-error hypothesis. We measured the precision of direction indication in waggle dances advertising
a nest site (typically a tree cavity, hence a target that is almost a point) and compared it with that of dances advertising
a food source (typically a flower patch, hence a target that covers an area). The precision of dances for a nearby nest site
was significantly higher than that of dances for an equidistant feeder. This was demonstrated four times with four colonies.
Our evidence therefore supports the hypothesis that the level of precision in the direction indication for nearby food sources
is tuned to its optimum without being at its maximum.
Received: 9 December 1998 / Received in revised form: 24 February 1999 / Accepted 12 March 1999 相似文献
55.
We examined the predictors of women's decisions to confront or report sexual harassment from a sample of 802 employed women, 142 of whom indicated they had been sexually harassed. Analyses of variance showed that personal assertiveness predicted the decision to confront the harasser. In addition, perceived procedural justice was associated with reporting sexual harassment. However, the direction of this relationship was opposite from that predicted: women who had reported sexual harassment through formal channels manifested lower perceptions of justice. © 1998 John Wiley & Sons, Ltd. 相似文献
56.
There is a very large research base in Applied Psychology on the importance of affective reactions on organizational functioning. Likewise, performance appraisal has been a heavily researched area within the field. The present field studies attempt to bridge our understanding of these two areas by studying the relationship between performance appraisal process variables and employee affective reactions. In particular, the current studies examine the effects of a relatively new performance appraisal variable, Perceived System Knowledge (Williams and Levy, 1992), on appraisal reactions and job attitudes. In both studies, there was a strong relationship between employees' levels of perceived system knowledge and their appraisal reactions and job attitudes. In Study 1, when controlling for performance rating, PSK was positively related to appraisal reactions, job satisfaction, and organizational commitment. The findings from Study 1 were replicated in the second study which employed a longitudinal design. In addition, Study 2 found a relationship between PSK and fairness perceptions. Implications for these results and suggestions for approaching performance appraisal from an organizational perspective are proposed. © 1998 John Wiley & Sons, Ltd. 相似文献
57.
Juliana Rangel Sean R. Griffin Thomas D. Seeley 《Behavioral ecology and sociobiology》2010,64(6):979-987
Animals that travel in groups must synchronize the timing of their departures to assure cohesion of the group. While most
activities in large colonies of social insects have decentralized control, certain activities (e.g., colony migration) can
have centralized control, with only a special subset of well-informed individuals making a decision that affects the entire
colony. We recently discovered that a small minority of individuals in a honeybee colony—an oligarchy—decides when to trigger
the departure of a swarm from its hive. The departure process begins with some bees producing the worker-piping signal (the
primer for departure) and is followed by these bees producing the buzz-run signal (the releaser for departure). In this study,
we determined the identity of these signalers. We found that a swarm’s nest-site scouts search for potential nest cavities
prior to the departure of the swarm from its hive. Furthermore, we found that the predeparture nest-site scouts are the sole
producers of the worker-piping signal and that they are the first producers of the buzz-run signal. The control of the departure
of a honeybee swarm from its hive shows how a small minority of well-informed individuals in a large social insect colony
can make important decisions about when a colony should take action. 相似文献
58.
The presence of Pb in the environment can cause significant health problems. These issues are exasperated when the lead is in a more amenable form for potential ingestion. This study investigates the potential human health risk from Pb in urban street dusts. The lead levels in urban street dust in major city centres in northern UK have been compared to levels determined in 35 cities around the world. With a few exceptions, it was noted that the mean Pb levels in this study exceeded those found in other cities worldwide. Samples (n = 15) of urban street dust were collected across five city centres, and specifically in areas in which pedestrians are likely to concentrate, as well as near historical buildings. Typical total lead concentrations across all sampling sites ranged from 306 to 558 mg/kg. The human health risk was assessed using oral bioaccessibility testing of the urban street dust. The mean oral bioaccessibility data, irrespective of site and sample location, were in the range 43 ± 9 %. The total and bioaccessible concentrations of lead were compared to the estimated tolerable daily intake (TDIoral) values based on unintentional soil/dust consumption. It is noted, in all cases, that the maximum estimated lead daily intake exceeded the TDIoral. An alternative approach for assessing the daily intake is proposed based on the actual measured air quality in selected cities on the same day as the sampled urban dust. 相似文献
59.
The risk from potentially toxic elements in playground soils is of concern to children’s health as a result of the potential for intentional or unintentional consumption. Current approaches, based on total potentially toxic element concentration, may overestimate the risk. This has resulted in the development and use of in vitro gastrointestinal extraction protocols. The concentration of arsenic (As) and lead (Pb) in soils collected from 12 schools in north-east England has been compared with Soil Guideline Values from seven countries as well as reported literature concentrations for playgrounds and recreational parks. It was found that the mean As concentration of 14.1 ± 5.4 mg/kg does not exceed the England residential Soil Guideline Value in any location, while the mean Pb concentration of 298 ± 380 mg/kg is high but not excessive. Both concentrations have been assessed using the maximum potential daily intake from soil that a child could ingest. Based on this protocol Pb does not exceed the tolerable daily intake in any playground location, while As is generally 10 times lower than the oral index dose in the majority of playgrounds. The oral bioaccessibility of As and Pb was investigated. It was concluded that the highest concentrations were determined in the gastric phase with maximum bioaccessibilities of 54 and 46 % for As and Pb, respectively. Here we have demonstrated that minimal risk arises from the investigated playgrounds provided that good personal hygiene is adhered to. 相似文献
60.
Most woody plants contain a diverse array of plant secondary metabolites (PSMs) that deter vertebrate herbivores. However,
mammalian folivores have evolved a complex of physiological and behavioural strategies to counter these compounds, leading
to the development of an “evolutionary arms race”. Marsupial folivores are ideal models to investigate the role of PSMs in
the interaction between the external foraging environment and the digestive physiology of mammalian herbivores, as we have
a very strong understanding of the diversity and modes of action of PSMs in Eucalyptus, as well as the mechanisms by which animals overcome the effects of these compounds. Studies of marsupial folivores have
benefited from the facts that: these herbivores subsist on relatively poor quality diets; they include feeding types from
specialist species such as the koala, to generalists; and life history factors such as maternal investment in reproduction
can be measured more easily than in eutherians. Here, we describe patterns of spatial variation in the types and distributions
of plant secondary metabolites in Australian forests and discuss how this variation influences foraging behaviour, habitat
selection and life history strategies in arboreal, folivorous marsupials. We also provide a summary of our understanding of
the mechanisms by which marsupials detect and regulate their intake of toxic compounds. While our examples are drawn largely
from studies of the interaction between marsupials and Eucalyptus, this knowledge is applicable to advancing our understanding of interactions in plant–mammal systems more broadly. We also
identify and discuss key areas that should be the focus of future research. 相似文献