COST OF WASH HOUSES AND COMFORT STATIONS AT RECREATION AREAS1

ABSTRACT: The cost of sanitary facilities is a major cost item in recreation areas at water resources projects. Often planners wish to analyze the tradeoffs in providing different levels of service at these areas. Cost functions are presented in this paper to enable the planner to make quick comparisons between different types of buildings, sizes, exterior treatments, and number of fixtures.     

A proposed MISA approach to setting limits and assessing compliance

Ontario's MISA program is intended to reduce all municipal and industrial waste loadings and to virtually eliminate the release of persistent toxic contaminants to surface waters. Based on 12-months of effluent monitoring data, effluent limit guidelines will be issued for 9 industrial and municipal sectors. The effluent limits will be based on the best available technology economically achievable and will involve extensive statistical analyses of the monitoring data. Proposed statistical methods for the derivation of effluent limits and assessing compliance are presented, along with brief information on the criteria for selection of pollutants and sample size requirements for the MISA effluent monitoring program. The proposed methods are not the policies of the Ministry of the Environment and may or may not be used for the development of effluent limits.     

Indoor radon/thoron levels and inhalation doses to some populations in Himachal Pradesh, India

It is well established that some areas of Himachal Pradesh (H.P.) state of India situated in the environs of the Himalayan mountains are relatively rich in uranium-bearing minerals. Some earlier studies by our group have indicated high levels of radon (>200 Bq m(-3)) in the dwellings. It is in this context that an indoor radon/thoron survey has been carried out in selected villages of four districts in the state of H.P. This survey has been conducted as a part of a national, coordinated project using twin chamber dosemeter cups designed by the Environmental Assessment Division (EAD), Department of Atomic Energy, Govt. of India. The track-etch technique is used for calibration of plastic detector LR-115 type-II which are employed for recording alpha tracks due to radon/thoron and their daughters. Year long radon/thoron data have been collected for seasonal correlations of indoor radon/thoron in the dwellings. The indoor radon levels have been found to vary from a minimum value of 17.4 Bq m(-3) to a maximum value of 140.3 Bq m(-3). The indoor thoron levels vary from a minimum value of 5.2 Bq m(-3) to a maximum value of 131.9 Bq m(-3). The year average dose rate for the local population varies from 0.03 microSv h(-1) to 0.83 microSv h(-1). The annual exposure dose to inhabitants in all the dwellings lies below the upper limit of 10 mSv given in ICRP-65.     

Spore inhibition-based enzyme substrate assay for monitoring of aflatoxin M1 in milk

A spore germination-based concept and its transformation into a field level prototype for monitoring aflatoxin M₁ (AFM₁) in milk was developed. Initially, 15 strains of Bacillus spp. procured from different culture collection were screened for AFM₁ sensitivity using spot assay and marker strain showing inhibition at 0.5 ppb was selected based upon maximum zone of inhibition. The selected strain B. megaterium 2949 was further screened for different enzymes activities and subsequently its spores were produced to an extent of 73.13% ± 3.197% in newly developed sporulation medium containing beef extract (0.0075% ± 0.0004%), yeast extract (0.015% ± 0.001%), peptone (0.0375% ± 0.0016%), and sodium chloride (0.0375% ± 0.0018%). A spore germination-based concept/ assay was optimized by immobilizing spores in eppendorf with pretreated milk (80°C/15 min) containing germinant and chromogenic substrate followed by incubation at 37°C. The appearance of sky blue color within real time of 45 min indicated spores germination and release of specific marker enzyme such as acetyl esterase and its specific action on chromogenic substrate which demonstrates absence of AFM₁ in milk. However, if there was no color change, presence of AFM₁ at 0.5 ppb MRL was denoted by Codex. The developed concept on AFM₁ detection was validated and a correlation of 0.97 was established with AOAC approved Charm 6602 and ELISA at Codex MRL with minimal false positive and negative results. The cost effective test has potential application in dairy farms, manufacturing, and R&D units for routine monitoring of AFM₁ in milk.     

Ein neuer Biotest mit der HefeSaccharomyces cerevisiae auf aquatische Toxizität

The fermentation activity of the yeastSaccharomyces cerevisiae is used as a bioindicator to detect aquatoxic effects. This is done by measuring the CO₂ production of yeast cells after a multiplication period affected by toxic substances. The concentration of toxicants diminishing the fermentation by 20% is taken as a characteristic value (EC₂₀). Organic compounds (unpolar and polar narcotics), inorganic salts (especially of heavy metals), surfactants and plant pesticides were tested. The results are compared so far as available with data of a ciliata assay usingTetrahymena pyriformis. A congruence of 90% and an equal sensibility of the assays resulted. The results of the yeast assay are therefore representative for ecotoxicological testing. The yeast assay is reproducible, easy to handle, and offers an alternative for sewage testing because sterile handling is not necessary.     

A comparative study on characterization of textile wastewaters (untreated and treated) toxicity by chemical and biological tests

Toxicity of textile wastewaters (untreated and treated) and their ingredient chemicals was quantified in terms of their chemical characteristics, fish (Gambusia affinis) mortality and end point growth responses of duckweed (Lemna aequinoctialis) in short-term bioassays. Other parameters of fish bioassay were erythrocyte morphology and its counts. Despite of a definite correlation between data of biological tests (LC/EC(50) values) with that of chemical tests, biological tests were found to be relatively more sensitive to both wastewaters and ingredient chemicals. Amongst all the examined parameters of test organisms, fish RBCs (morphology and counts) sensitivity to pollutants in the wastewaters was usually maximum and therefore, their study should be included in the routine fish bioassay. Other advantage of biological test such as on Lemna is even detection of eutrophic potential of wastewaters, as noted at their higher dilutions. The ingredient chemicals (major) contributing maximum toxicity to textile dye wastewater were, acids (HCl and H(2)SO(4)), alkali (Na(2)O SiO(2)), salt (NaNO(2)) and heavy metal (Cu), whereas dyes (4) were relatively less toxic.     

Biochemical biomarkers in environmental studies—lessons learnt from enzymes catalase,glutathione <Emphasis Type="Italic">S</Emphasis>-transferase and cholinesterase in two crustacean species

Background, aim and scope  

For reliable environmental risk assessment of pollutants, knowledge on the effects at different levels of biological organisation is needed. During the early days of biomarker research in environmental studies approximately two decades ago, biochemical biomarkers were considered as the most promising tool for such purposes. Among these, three enzymes have often been studied: catalase (CAT), glutathione S-transferase (GST) and cholinesterase (ChE). However, despite their intensive research, their measurements in invertebrates have not been commonly applied in environmental risk assessment (ERA) or for regulatory purposes.     

Seasonal air quality profile of inorganic ionic composition of PM10 near Taj Mahal in Agra, India

Atmospheric aerosols and their impacts on the environment particularly on human health is an issue of significant public and governmental concern. Though studies on air quality related to total suspended particulate matter have done by various authors in India, yet respirable suspended particulate matter (PM₁₀) is not characterized so far particularly in a historical and world heritage city like Agra. This study presents seasonal variation in mass levels of PM₁₀ and its ionic composition. PM₁₀ samples were collected in the proximity of Taj Mahal and subjected to chemical analysis using ion chromatography technique. The preliminary findings reveal that the 24-h average of PM₁₀ mass level varies from 115 to 233, 155 to 321, and 33 to 178 μg/m³, respectively, in summer, winter, and rainy seasons indicating critical pollution situation. These values are very much higher than the National Ambient Air Quality Standards of 75 μg/m³ (prescribed by Central Pollution Control Board, India) in both of summer and winter seasons whereas quite near the permissible limits in rainy season. The equivalent ratios of NH₄ ⁺ to nonsea salt SO₄ ^2? and NO₃ ^? and ∑Cations to ∑Anios were found to be greater than unity indicating high source strength of ammonia and alkaline nature of aerosols. The study suggests the need for continuous and long-term systematical sampling and detailed physiochemical analysis of PM₁₀ and also to know the characteristics of PM in background areas for better understanding of the emission sources.