Surveillance for adverse health effects following a chemical release in west virginia

On 11 August 1985, a large inadvertent release occurred from a chemical manufacturing plant in Institute, West Virginia. The emission was related to overheating of a storage tank that contained chemicals for the production of aldicarb, a cholinesterase inhibiting pesticide. As a result of this emission, 136 persons were treated in five local emergency rooms, 29 of whom were hospitalized for one or more days, mostly for observation. No fatalities resulted. Interviews with treated persons and non-treated community residents confirmed that the health effects were transient and compatible with exposure to irritating vapors rather than with exposure to methyl isocyanate (a chemical used in the process) or to aldicarb. Only 5% of the treated persons and 5% of the community residents surveyed were adequately warned of the emission by the plant siren. Because persons in the nearby community are potentially vulnerable to chemical injury, we recommend the development of an improved warning system, a formal evacuation procedure, and other measures to limit exposure of persons in this area in the event of a future release.     

The importance of free amino acids to the energy metabolism of eggs and larvae of turbot (Scophthalmus maximus)

A study was undertaken to establish the role of free amino acids (FAA) in aerobic energy dissipation in embryos of turbot (Scophthalmus maximus) which contain an oil globule in the egg. Laboratory-reared developing eggs and larvae (15°C, 34 salinity) were measured for oxygen uptake, ammonia excretion, contents of FAA, protein, and ammonium, and volumes of yolksac and oil globule. Newly spawned eggs from different batches contained 55 to 90 nmol egg^–1 of FAA. Resorption of FAA occurred in parallel with the consumption of yolk. Resorption of the oil globule, however, occurred predominantly after hatching and mainly after yolk resorption. The combined data suggest that approximately 70% of the FAA are utilized as an energy substrate, while the rest are polymerized into body proteins. FAA become a significant energy substrate in the early egg stage and account for 100% of the aerobic energy dissipation 2 d after Fertilization then decrease to ca. 60% at the time of hatching. Lipids derived from the oil globule seem to be the main fuel after hatching and account for ca. 90% of the energy dissipation at the onset of first-feeding. Thus, the energetics of fish embryos which contain an oil globule seems to be different from those that depend exclusively on the nutritional reserves of the yolk.     

Studies on feeding repellents for fishes and shellfishes

The present study on chemotaxis activity was conducted between May 1989 and March 1990. In order to establish a chemotaxis index, with special reference to a repellence index of food for black abalone Haliotis discus, a logistic curve y=g/{1+ exp [-r(j-a)]}, where g, r and a are constants, was applied to time-courses of abalone responses to fresh and spoiled algal extracts. The time-courses were respresented by the number of individuals (y _i) displaying three behaviors (entering. leaving and remaining in a test compartment with algal sample) over time (j=1 to 10, 2-min intervals). Repellence and attraction specimens were prepared from a homogenate of green laver Ulva pertusa allowed to spoil for 2, 4 and 6 d at 40°C, and from fresh homogenate, respectively. Of 32 time-courses (estimated according to a method whereby the observed number was weighted by j for each behavior, involving 30 individuals), 32 entered, 19 left and 19 remained time-courses fitted the curve at the 0.10 level, as determined by a chi-square test. A general evaluation of repellence and attraction, determined by observable behavior, agreed well with the difference in a between the dummy (control) and specimen curves, and with the ratio of a in the dummy curve to a in the specimen curve, for logistic curves fitted to the entred time-course. The difference was >0 and the ratio was >1 in the repellence index, and <0 or <1 respectively in the attraction index. The validity of the indexes was ascertained using food specimens prepared from three kinds of algae.     

Relationships between porewater nutrients and seagrasses in a subtropical carbonate environment

The feeding, diet and egg production of the copepod Acartia tonsa were dermined during ten experiments in Los Angeles Harbor, California, between November 1986 and October 1987. Copepods were incubated in situ, in quasi-natural food environments. Water temperatures ranged from 14.6 to 21.5°C. Particulate organic carbon and nitrogen (POC and PON) were high (534 to 3710 g Cl^-1, 51 to 459 Nl^-1) but dominated by small (<8 m diam) particles. Plankton (phytoplankton and microzooplankton) C-biomass composed about 10% of the total POC and was usually dominated by particles >8 m. Plankton biomass was always low. Daily ingestion rates ranged from 3 to 96% of body C; egg production ranged from 4 to 35% of body carbon. Mean ingestion and egg production rates during spring-summer were 1.9 and 1.5 times higher than average for the entire study, respectively. The average gross efficiency of egg production for the study was 80%; the spring-summer mean was 41%. Bivariate and multiple-regression analyses revealed that the ingestion rate was dependent upon both temperature and food availability, but that, below 21°C, egg production depended more upon temperature than upon food concentration. To detect dietary preferences, the composition of diet was compared with that of the food supply. Selective feeding was infrequent, but the diet was often dominated by dinoflagellates and ciliates. It would appear that within metabolic limits governed by temperature, the feeding response of A. tonsa is dependent upon food concentration, while egg production depends more on qualitative attributes of the food supply.     

Persistent “upwelling shadows” and their influence on zooplankton distributions

Physical (temperature and salinity) and biological (zooplankton) properties were sampled over a 15 mo period in 1988 and 1989 to monitor nearshore environmental variability in northern Monterey Bay, California, USA. During the upwelling seasons of 1988 and 1989, there were two distinct bodies of water along the sampling transect. The offshore water body was characterized by recently upwelled water (typically <12°C) while the nearshore body was significantly warmer (approaching 16°C). A sharp thermal gradient (=front) separated the two water bodies. This front persisted 6.5 km from shore throughout the upwelling season of 1988 and was present again in 1989. Zooplankton-assemblage analysis confirmed the presence of two distinct regions in northern Monterey Bay. We refer to this phenomenon as an upwelling shadow, which is the result of the advection of recently upwelled water bearing nutrients and larvae past coastal recesses which are equatorward of upwelling centers.     

Effects of high temperature on larval development and metamorphosis of Arachnoides placenta (Echinodermata: Echinoidea)

Adults of the sea urchin Arachnoides placenta (L.) were induced to spawn, and eggs were fertilized at 28°C in September 1989. After 5 min, eggs were transferred to 28, 31, 34, or 37°C and reared to metamorphosis. Embryos were observed at 20-min intervals during the first 2 h; larvae were observed daily. The cleavage was higher at higher temperatures. Embryos reared at 28°C were still at the 4th cleavage (16-cell stage) after 100 min, while those at 34°C had reached the 5th cleavage (32-cell stage). All embryos reared at 37°C died on the second day. Incidence of abnormality was 20 to 30% at 28 and 31°C, 48% at 34°C, and 77% at 37°C. The 8-arm stage was reached after 4 d at 28°C, 3 d at 31°C and 2 d at 34°C. Larvae displayed decreasing body length and arm length with increasing temperature. Larvae at 31°C have relatively long arms, as a result of a decrease in body length, not because of increased arm length. Incidence of metamorphosis was 43.9±1.7% (mean/plusmn;SD) at 28°C, 24.5±1.9% at 31°C, and 5.3% at 34°C. The size of metamorphosed juveniles was significantly larger at 28°C than at 31 and 34°C. Temperatures of 31°C negatively affect larvae and juveniles of the sand dollar.     

The effect of selenium on fluoride — clay interactions: Possible environmental health implications

Previous epidemiological studies have shown that dental fluorosis is endemic in the lowland, dry zone of Sri Lanka, which is considered to be an area in which excessive quantities of fluorides are present in the drinking water supplies. It has been found that kaolinitic clay forms a suitable raw material in the defluoridation of water.It is shown that there is a noticeable effect of selenium and media pH on the reactions involved in the interaction of fluoride with clay. In this study, 1 mM fluoride solutions containing SeO ₃ ^2– (selenite) concentrations of 0 mM, 0.1 mM, 0.5 mM and 1 mM were used in the reactions with kaolinitic clay. The effect of pH was monitored in the range 4 to 8. It was observed that fluoride adsorption was maximum at a pH of 5.6 without either SeO ₃ ^2– or SeO ₄ ^2– , the adsorption capacity being 15.2 mol F^– g^–1 clay. However, when the SeO ₃ ^2– concentration was increased up to 0.5 mM at this optimum pH, the adsorption capacity reduced to 12.8 mol F^– g^–1 clay. Monitoring of the effect of SeO ₄ ^2– and media pH on fluoride adsorption showed that when the SeO ₄ ^2– concentration increases from zero to 0.1 mM, there is a reduction of fluoride adsorption capacity. However, when the SeO ₄ ^2– concentration is further increased from 0.1 mM to 1.0 mM, there was an increase in the fluoride adsorption capacity, indicating a more consistent effect of SeO ₃ ^2– on fluoride-kaolinitic clay interaction than SeO ₄ ^2– .Fluoride concentrations in drinking water supplies have a marked effect on dental health and the geochemistry of selenium appears to play an important role in the geochemical mobility of fluoride ions.     

Critical loads for Finnish lakes: comparison of three steady-state models

Three simple steady-state water-chemistry models are used to calculate critical loads of sulfur for lakes in Finland. Because of the high concentrations of organic matter in Finnish lakes, the influence of organic anions on the calculation of critical loads has been given special attention. The first two methods are well known ion-balance methods which have been used in many previous lake-acidification studies. The third method, developed for this study, includes the numerical solution of equilibrium equations for organic anions, inorganic carbon species and inorganic monomeric aluminum. The original pH and aluminum concentration of the lakes are estimated with this model, and a method to estimate the original acid neutralizing capacity (ANC) by simulating a Gran-titration is also tested on the lake data. Uncertainty in the predictions is estimated by varying the most critical model parameters.     

Orientation ofRhizophagus grandis (Coleoptera: Rhizophagidae) to oxygenated monoterpenes in a species-specific predator-prey relationship

Summary The predator,Rhizophagus grandis, is linked to its specific prey,Dendroctonus micans, by semiochemical signals regulating oviposition and long range orientation. A mixture of simple oxygenated monoterpenes [(–)—fenchone, (–)—pinocamphone, rac. camphor, terpinene-4-ol, borneol, fenchol and verbenone], identified from the frass ofD. micans has been found to be extremely active in a flight windtunnel as a long—range attractant for the predator. The mixture elicited 84 % of the response to larval frass of the prey. Excluding pinocamphone from the mixture did not influence its activity. Also, changing the absolute configurations of some of the components (fenchol, terpinene-4-ol and borneol) did not influence the predators' response. However, the addition of (–)—-terpineol increased the attractivity of the synthetic blend to almost that of larval frass of the prey. Ecological implications of the identified semiochemicals and their use in pest management are discussed.     

Effect of nickel on some aspects of protein metabolism in the gill and kidney of the freshwater fish, Cyprinus carpio L

Nickel has an adverse effect on some aspects of protein metabolism of the freshwater fish, Cyprinus carpio. The main changes observed were: (a) Decrease in soluble, structural and total proteins, AlAT and AAT activities with an increase in the levels of free amino acids, protease and GDH activities and ammonia in the gill and kidney at 1, 2, 3, and 4 days of exposure to a lethal concentration, 40 mg litre(-1) of nickel; (b) Increase in soluble, structural and total proteins, free amino acids and the activities of protease, AlAT, AAT and GDH with a decrease in ammonia and urea in these organs at 1, 5, 10 and 15 days of exposure to sublethal concentration, 8 mg litre(-1); (c) The magnitude in these changes increased over time with both concentrations of the metal, and was more marked in gill than in kidney.