An anaerobic continuous-flow fixed-bed reactor sustaining a 3-chlorobenzoate-degrading denitrifying population utilizing versatile electron donors and acceptors

An anaerobic continuous-flow fixed-bed column reactor capable of degrading 3-chlorobenzoate (3-CBA) under denitrifying conditions was established, and its rate reached 2.26 mM d(-1). The denitrifying population completely degraded 3-CBA when supplied at 0.1-0.54 mM, but its activity was partly suppressed when 3-CBA was supplied at 0.89 mM. Nitrate was concomitantly consumed throughout the operation of the reactor, the amount of which was similar to or up to 35% higher than the theoretical stoichiometric value that was calculated by assuming that 3-CBA degradation is coupled with denitrification. Batch incubation experiments proved that nitrate is strictly required for 3-CBA degradation in the absence of molecular oxygen. The population also degraded 3-CBA aerobically. Benzoate and 4-CBA were degraded under denitrifying conditions as well as 3-CBA, but 2-CBA was not. Considering that the previously reported denitrifying 3-CBA-degrading cultures do not exhibit 4-CBA degradation under denitrifying conditions, nor aerobic 3-CBA degradation [FEMS Microbiol. Lett. 144 (1996) 213, Appl. Environ. Microbiol. 66 (2000) 3446], the microbial population developed in this experiment was physiologically versatile with respect to the utilization of both electron donors and electron acceptors.     

Environmental impacts of shore-parallel breakwaters along Nagahama and Ohgata, District of Joetsu, Japan

A combination of sedimentological analysis of beach sands, field surveys and the assessment of the empirical methods for the breakwater design is used to investigate the impacts of offshore breakwaters on beach morphology along Nagahama and Ohgata. The results indicate that the nearshore sedimentation has been substantially disrupted due to the features formed behind the breakwaters stopping littoral transport, which led to sediment accretion on the updrift side of the breakwaters, and erosion on their downdrift side. Sedimentological analysis of beach sands made it possible to recognize the dominant process at the sections considered. In fact, the occurrence of strong offshore-going currents, the removal of finer mean grain size and the predominance of high-density heavy minerals led us to deduce that the two sections undergo a natural sedimentary imbalance.