Selecting peptide ligands of microcystin-LR from phage displayed random libraries

In the present study, we investigated to find novel ligands for low molecular weight environmental toxin, microcystin-LR (MC-LR) by using phage display technology. Two random libraries, displaying linear 12-mer peptides and cyclic 7-mer peptides, were screened against the immobilized target respectively. After three rounds of panning, phage clones that recognized microcystin-LR specifically were obtained from both the linear and the constrained libraries, proved by enzyme-linked immumosorbent assays and immunoprecipitation assays. DNA sequencing indicated that peptides displayed on some of the selected clones shared consensus sequences. Compared with traditional methods, this approach provided a cheaper and more rapid alternative to screen specific ligands for microcystin-LR. Moreover, since it is rather difficult to take small molecules as targets of phage display libraries, the success of this experiment expanded the applications of phage display technology, and provided a new avenue to study environmental small molecular toxins.     

Automated radioxenon monitoring for the comprehensive nuclear-test-ban treaty in two distinctive locations: Ottawa and Tahiti

In preparation for verification of the Comprehensive Nuclear-Test-Ban-Treaty, automated radioxenon monitoring is performed in two distinctive environments: Ottawa and Tahiti. These sites are monitored with SPALAX (Systeme de Prelevement d'air Automatique en Ligne avec l'Analyse des radioXenons) technology, which automatically extracts radioxenon from the atmosphere and measures the activity concentrations of (131m,133m,133,135)Xe. The resulting isotopic concentrations can be useful to discern nuclear explosions from nuclear industry xenon emissions. Ambient radon background, which may adversely impact analyser sensitivity, is discussed. Upper concentration limits are reported for the apparently radioxenon free Tahiti environment. Ottawa has a complex radioxenon background due to proximity to nuclear reactors and medical isotope facilities. Meteorological models suggest that, depending on the wind direction, the radioxenon detected in Ottawa can be characteristic of the normal radioxenon background in the Eastern United States, Europe, and Japan or distinctive due to medical isotope production.     

海洋石油开发工程环境影响后评价初探

环境影响后评价是环境影响评价的延伸和发展,将项目后评价的思想引入项目环境管理体系,发展了环境影响后评价的概念。目前环境影响后评价理论和方法体系尚处于发展初期的探索阶段。文章论述了建设项目环境后评价的概念、作用和评价原则及其与环境影响评价的区别,对海洋石油开发工程的环境影响及其影响后评价方法进行了探讨,并以北部湾油田开发工程作为实例进行了说明。     

在线浓缩-流动注射分光光度法测定海水中痕量的镉

本文针对海水中镉含量低、海水基体干扰严重等特点，建立了在线浓缩一流动注射分光光度法测定海水中痕量镉的新方法。优化了Cd（Ⅱ）的显色条件、浓缩条件和解吸条件，在最佳检测条件下，Cd（Ⅱ）的检出限为0．02μg/L，线性范围为0．05～2μg/L，相对标准偏差RSD为2.74％（n=g，Ccd（Ⅱ）=1μg／L）。将该方法应用于实际海水中Cd（Ⅱ）的测定。结果满意。     

不同植物对富营养化水体净化的静态试验研究

采用浮床无土裁培美人蕉等6种植物进行了处理富营养化水体的静态试验.结果表明:植物在水中均能正常生长,对水体具有明显的净化效果,NH3-N也有一定的下降,并且具有良好的景观效应.研究结果为发展利用陆生植物治理富营养化水域提供了新的途径.     

山西霍州西部地裂缝地质灾害成因分析

自20世纪90年代以来，山西省霍州西部地区出现了大量地裂缝，破坏了许多房屋和耕地，并且改变了地下水资源的空间分布，严重影响了当地人民的日常生活和地区经济发展。由于该地区地裂缝的成因尚不清楚，防治措施难以制订，其影响范围内的人群、房屋、耕地、水资源仍然受着地裂缝地质灾害的威胁。基于此种状况，在调查霍州地区采空塌陷地裂缝时空分布的基础上，结合区内区域地质、水文地质、工程地质及人类经济工程活动等条件，分析了地裂缝形成的主要原因。分析结果认为，采煤形成的采空区和采煤排水造成的地下水位下降是地裂缝形成的主要原因，雨水的冲蚀加速了地裂缝的扩展，确定了该地区地裂缝的非构造属性，并据此提出了相应的治理措施。     

Mercury in surface waters of a macrotidal urban estuary (River Adour, south-west France)

Surface waters were collected in the River Adour estuary (south-western France) during different sampling periods from 1998 to 2001 in order to investigate the phase distribution and speciation of methylmercury (MeHg⁺). Although a high variability is observed, significant higher average concentrations of total MeHg⁺ (dissolved and particulate) are measured during the warm seasons, at 4.40±8.18 pM and 3.90±1.87 pM for July 1998 and September 1999, compared with the winter seasons, with concentrations at 0.99±2.85 pM and 1.00±1.75 pM for February 1998 and February 2001, respectively (one-tailed t-test, P=0.01). The seasonal variations are explained with enhanced bacterial activity during summer and sedimentation/resuspension phenomena. Additionally, signi-ficant longitudinal variations of the MeHg⁺ concentrations are observed. The highest levels in both dissolved and particulate fractions are found within the downstream urban estuarine area. This can be explained by the high methylation potential of the sediments, but direct anthropogenic inputs of MeHg⁺ from specific discharge points cannot be neglected. Biogeochemical factors like phytoplankton biomass and salinity also show a relationship with MeHg⁺ partitioning in the surface waters of the Adour estuary.     

全球海岸带环境问题现状和相互联系

引言 "亮点"是由城镇发射出来的光经过聚集而形成,在地球卫星的夜间景象上可以观察到.它们主要位于海岸带中,是反映地球上人口分布的良好标志[1].海岸带拥有各种不同的生物生境、重要居民点以及宝贵资源的产地.     

Lead contamination and isotope signatures in the urban environment of Hong Kong

The source and the extent of Pb pollution in the urban environment of Hong Kong were investigated at five different urban settings selected on the basis of their annual average daily traffic (AADT) varying from less than 100 to 61,700. In addition, a small distant island without any traffic was selected to establish the possible baseline values. The surface environmental samples studied consisted of street and tunnel dusts, gully sediments, and a limited number of roadside topsoils. The analytical results clearly indicated variable degrees of Pb contamination in these urban settings. However, the level of contamination varied significantly among different types of samples collected at the same location. Pb concentrations of roadside topsoils (79+/-22 micrograms/g) and gully sediments (278+/-88 micrograms/g) were lower than those of the corresponding road dusts (327+/-54 micrograms/g). The Pb isotope compositions in different urban settings varied considerably. The bedrock in the small island had the lowest Pb concentration (12 micrograms/g) but with the highest 206Pb/207Pb ratio (1.2206), whereas the tunnel ceiling dusts with the highest level of Pb (1410 micrograms/g) had the lowest 206Pb/207Pb ratio (1.1062). Despite the significant differences in vehicle types and traffic volumes, and the presence of several different petroleum retailers in Hong Kong, the Pb isotope ratios of road dusts (206Pb/207Pb: 1.1553+/-0.0043, 208Pb/207Pb: 2.4408+/-0.0084) varied within a relatively narrow range among all the five urban sampling sites. On the other hand, the Pb isotopic compositions of gully sediments (206Pb/207Pb: 1.1515+/-0.0145, 208Pb/207Pb: 2.4322+/-0.0198) varied noticeably within the same setting, but were reasonably comparable across the different study sites. In general, the 206Pb/207Pb ratios of road dusts can be used to estimate the direct contribution from automobile emissions, whereas those of gully sediments might reflect the effects of the mixing of different anthropogenic sources. The Pb isotope signatures in the urban environment of Hong Kong clearly suggested that anthropogenic Pb in the environment originated from Pb ore with a low 206Pb/207Pb ratio (such as the Australian Pb ore and similar sources in Southeast Asia) were significantly different from those of the anthropogenic Pb present in the neighboring Pearl River Delta (PRD) region.