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11.
Vandenhove H Van Hees M Wannijn J Wouters K Wang L 《Environmental pollution (Barking, Essex : 1987)》2007,145(2):577-586
The present study aimed to quantify the influence of soil parameters on uranium uptake by ryegrass. Ryegrass was established on eighteen distinct soils, spiked with (238)U. Uranium soil-to-plant transfer factors (TF) ranged from 0.0003 to 0.0340kgkg(-1). There was no significant relation between the U soil-to-plant transfer (or total U uptake or flux) and the uranium concentration in the soil solution or any other soil factor measured, nor with the U recovered following selective soil extractions. Multiple linear regression analysis resulted in a significant though complex model explaining up to 99% of variation in TF. The influence of uranium speciation on uranium uptake observed was featured: UO(2)(+2), uranyl carbonate complexes and UO(2)PO(4)(-) seem the U species being preferentially taken up by the roots and transferred to the shoots. Improved correlations were obtained when relating the uranium TF with the summed soil solution concentrations of mentioned uranium species. 相似文献
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Basinas I Sigsgaard T Heederik D Takai H Omland Ø Andersen NT Wouters IM Bønløkke JH Kromhout H Schlünssen V 《Journal of environmental monitoring : JEM》2012,14(2):604-614
Studies on personal dust and endotoxin concentrations among animal farmers have been either small or limited to a few sectors in their investigations. The present study aimed to provide comparable information on the levels and variability of exposure to personal dust and endotoxin in different types of animal farmers. 507 personal inhalable dust samples were collected from 327 farmers employed in 54 pig, 26 dairy, 3 poultry, and 3 mink farms in Denmark. Measurements in pig and dairy farmers were full-shift and performed during summer and winter, while poultry and mink farmers were monitored during 4 well-defined production stages. The collected samples were measured for dust gravimetrically and analyzed for endotoxin by the Limulus amebocyte lysate assay. Simple statistics and random-effect analysis were used to describe the levels and the variability in measured dust and endotoxin exposure concentrations. Measured inhalable dust levels had an overall geometric mean of 2.5 mg m(-3) (range 相似文献
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Vandenhove H Van Hees M Wouters K Wannijn J 《Environmental pollution (Barking, Essex : 1987)》2007,145(2):587-595
Present study aims to quantify the influence of soil parameters on soil solution uranium concentration for (238)U spiked soils. Eighteen soils collected under pasture were selected such that they covered a wide range for those parameters hypothesised as being potentially important in determining U sorption. Maximum soil solution uranium concentrations were observed at alkaline pH, high inorganic carbon content and low cation exchange capacity, organic matter content, clay content, amorphous Fe and phosphate levels. Except for the significant correlation between the solid-liquid distribution coefficients (K(d), L kg(-1)) and the organic matter content (R(2)=0.70) and amorphous Fe content (R(2)=0.63), there was no single soil parameter significantly explaining the soil solution uranium concentration (which varied 100-fold). Above pH=6, log(K(d)) was linearly related with pH [log(K(d))=-1.18 pH+10.8, R(2)=0.65]. Multiple linear regression analysis did result in improved predictions of the soil solution uranium concentration but the model was complex. 相似文献
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Bart Wouters Marijn Nijssen Gertjan Geerling Hein Van Kleef Eva Remke Wilco Verberk 《Journal of Coastal Conservation》2012,16(1):89-99
Encroachment of tall grasses and shrubs in coastal dunes has resulted in loss of vegetation heterogeneity. This is expected
to have negative effects on animal diversity. To counteract encroachment and develop structural heterogeneity grazing is a
widely used management practice. Here, we aim to functionally interpret changes in vegetation composition and configuration
following grazing management on habitat suitability for sand lizards. Aerial photographs taken over a period of 16 years were
used to quantify changes in vegetation composition. A GIS-based method was developed to calculate habitat suitability for
sand lizards in a spatially explicit manner, encompassing differences in vegetation structure and patch size. From 1987 to
2003 dune vegetation shifted from small patches of moss and sand to larger patches covered by shrubs and grasses. Grazing
management did not have any significant effect on the overall level of heterogeneity, measured as habitat suitability for
sand lizards. However, on a more local scale highly suitable patches in 1987 were deteriorating whereas unsuitable patches
became more suitable in 2003. This inversion results from a broad shift with shrubs being a limiting habitat element in 1987
to sandy patches being the limiting element in 2003. Future changes are believed to negatively impact sand lizards. The habitat
suitability model has proven to be a useful tool to functionally interpret changes in coastal dune vegetation heterogeneity
from an animal’s perspective. Further research should aim to include multiple species operating on different scale levels
to fully capture the natural landscape dynamics. 相似文献
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The objective of the study was to investigate the importance of protozoan predation as a biological removal mechanism in sand filters used for purification of bacteria from wastewater. Eleven sand filter columns were seeded with a high dose of wastewater (70 mm d(-1)) and a high concentration (10(8) colony forming units [CFU] mL(-1)) of Aeromonas hydrophila (American Type Culture Collection [ATCC] 14715) for a period of 30 d. Water samples from three filter outlets were analyzed for the concentration of A. hydrophila. In addition, one filter column was sacrificed each sampling day for the quantification of A. hydrophila, culturable bacteria (heterotrophic plate counts, HPC), total bacterial counts, and protozoa in the sand. The mean removal efficiency of A. hydrophila in the sand filter columns was 4.7 log units. The concentration of A. hydrophila in the sand filter effluent, however, had a clearly time-dependent pattern from high (log 6) and unstable concentrations to low and more stable levels (log 2). The removal efficiency of A. hydrophila correlated significantly (P = 0.0005, r2 = 0.6) with numbers of protozoa in the sand filters. Significantly higher (P < 0.05) concentrations of A. hydrophila were observed in sand filter effluents from columns treated with the protozoan inhibitor cycloheximide, compared with nontreated columns. Results from the present study show that protozoan grazing plays an important role as a bacterial removal mechanism in sand infiltration systems. 相似文献
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Koets M Renström A Zahradnik E Bogdanovic J Wouters IM van Amerongen A 《Journal of environmental monitoring : JEM》2011,13(12):3475-3480
Allergy to rodent proteins is common among laboratory animal workers. Sensitive methods to measure exposure to these allergens have been developed. These assays are, however, expensive, time-consuming, and require a laboratory facility and methodological expertise. A simple method to screen for allergen spread, or to test whether hygiene standards are maintained, would be useful. Lateral flow immunoassays (LFIAs) are especially suited for field settings; the tests are simple and results are visible within minutes. LFIAs were developed for detection of the rodent urinary allergens Mus m 1 and Rat n 1. Pilot studies were performed in animal facilities in three countries using both extracts from airborne dust samples and samples collected by wiping surfaces. For comparison and determination of sensitivity, the concentrations of rodent urinary allergens in the samples were also measured using enzyme immunoassays (EIAs). The LFIAs for rat and mouse urinary allergens had a detection limit of 31 pg allergen per mL in a buffer system with purified allergen standards. Results of environmental dust extracts tested in LFIAs correlated well with levels obtained using EIAs. Spread of rodent allergens, or non-adherence to hygiene around laboratory animal facilities, may aggravate rodent allergy. Using a simple, sensitive one-step assay, allergens can be detected to prevent allergen exposure. The results reveal that the rapid assays are suited for on-site demonstration of exposure to rodent allergens, and thus, useful in occupational hygiene practice. 相似文献