Microbiological and mycotoxicological contamination in Slovenian primary grain production

This study was aimed at investigating the contamination of grains, which are grown and used for animal diets by Slovene farmers. A total of 107 samples were examined for mesofilic aerobic molds and yeasts. The leading contaminants among molds were from Fusarium spp., detected in 73 samples, mostly in maize (50). In accordance with the European Feed Microbiology Organisation criteria, 68% of all tested samples were of the quality level I, with normal microbiological flora, and 9% of samples were estimated as quality level IV and not suitable for animal feed. Using liquid and gas chromatography methods, the presence of various mycotoxins (expressed as 12% of moisture content) was found in 77% of all samples; mostly deoxynivalenol (71%) in concentration of 150–14,420 µg kg^?1, followed by zearalenone (32%, 20–640 µg kg^?1), fumonisin B1 (31%, 10–4863 µg kg^?1), fumonisin B2 (22%, 10–1629 µg kg^?1), while HT-2 and T-2 toxins were found in low concentration in two samples, and aflatoxin B1 and ochratoxin A in one sample. By statistical evaluation, significant positive correlations were noted between the presence of deoxynivalenol and zearalenone as well as between fumonisin B1 and B2.     

Environmental human silver exposure

Environmental exposure to silver (Ag) was assessed in occupationally non-exposed adult human population by analyzing Ag in the hair (H?·?Ag) and whole blood (WB?·?Ag). H?·?Ag was analyzed in 311 (123 men, M; 188 women, W); while WB?·?Ag was determined in 235 of these individuals (90 M, 145 W). Women had more H?·?Ag than men (M 0.05 vs. W 0.076), whereas WB?·?Ag concentrations in men and women were not significantly different. A natural distribution of the median derivatives was utilized to generate the dataset to fit the logistic sigmoid curve to assess the current human body burden of environmental Ag population exposure for M and W separately. The H?·?Ag (µg?g^?1) below 0.0105 for M and 0.0145 for W, reflects low level of environmental Ag exposure. The adaptive physiological saturation phase followed where H?·?Ag rose rapidly, first for M and then for W in parallel with biological assay. Both parallel saturation curves converged and plateaued at 0.215 for M and 0.965 for W (µg?g^?1). The current level of human environmental Ag exposure is low, but cases of high Ag exposure occurred sporadically. In conjunction with the medical histories overt clinical neural toxicity may be expected for H?·?Ag at 4?µg?g^?1and higher. There were no significant correlation between the H?·?Ag and WB?·?Ag.     

Treatment and skin decontamination of sarin intoxicated mice

Abstract

This study aimed at evaluating the efficacy of oral treatment and skin decontamination with the mineral cationic carrier in sarin-intoxicated mice. Mice were contaminated with increasing percutaneous sarin doses and decontaminated. Furthermore, the surviving mice were treated per os. The behavioral patterns of the surviving animals were monitored for 72?h, including the animals’ posture, desire for food and water, and approach and touch response. The results showed the mineral cationic carrier to be capable of efficiently decontaminating animals percutaneous poisoned with triple lethal dose. The comparison of behavioral pattern of control animals against those treated per os showed statistically significant differences. Oral treatment with mineral cationic carrier may be a detoxification tool against nerve agents’ poisoning, and this merits further research.     

Catalytic decomposition of hypochlorite ion in petrochemical plant waste water

During the incineration of the chlorinated hydrocarbons originating as the side products in vinyl chloride plant, hypochlorite ion appears as a pollutant in plant waste water. To remove it to the permitted level of 2 mg/l the process of the catalytic oxidation by cobalt (II, III) oxide, followed by sodium sulphite treatment has been chosen. It was shown that the above choice gives satisfactory results. The influence of some parameters such as retention time, effluent pH and temperature versus removal efficiency have been determined.     

Application of Synchronous Fluorescence Spectroscopy for Characterization of the Aromatic Hydrocarbons in Sediments of the Middle Adriatic

UV-fluorescence spectroscopy method with synchronous mode of scanning was used to characterize the types of aromatic hydrocarbons in surface sediments. the sampling stations were located on two transverses between Yugoslavian and the Italian coasts in the middle of the Adriatic Sea. the preparation of sediment samples was made according to IOC/UNESCO (1982). Synchronous excitation/emission scanning were done at wavelengths from 236/260 to 516/540 nm. Since the wavelength of maximum emission is a function of fused aromatic rings in a molecule, the fluorescence spectra of each sample were divided into three intervals: 300–340, 340–400, over 400 nm, corresponding to compounds with 2, 3 and 4,5 and more rings respectively.

Large qualitative differences were established between sediment samples. PAH with 5 and more rings are more prevalent near the Italian coast.     

The effect of macrophytes on retention times in a constructed wetland for wastewater treatment

Retention times of treated water in a constructed wetland (CW) with horizontal subsurface flow were determined both in the vegetative and non-vegetative periods of 2005. Tracer experiments were performed using fluorescein, an organic compound detectable at extremely low concentrations. Nominal and tracer retention times were determined and compared. Winter tracer retention time (TRT 194 h) and nominal retention time (nHRT 190 h) were nearly exactly equal, while summer TRT (335 h) was approximately twofold higher than nHRT (158 h). Residence time distribution function (RTD) was used to compare retention times obtained for the vegetative and non-vegetative periods. The obtained results document a significant influence of dense common reed vegetation on retention characteristics of the studied system. Common reed can convert a significant volume of water from liquid to gas via evapotranspiration (ET) and thus prolong water retention times in the system. This is very important both technically and ecologically. Longer retention times mean a longer time for microbiological decay of wastewater. Water converted from liquid to gas causes cooling of the microclimate, which is very important, especially in intensively cultivated areas with a lack of water.     

Tracing the sources of gaseous components (<Superscript>222</Superscript>Rn,CO<Subscript>2</Subscript> and its carbon isotopes) in soil air under a cool-deciduous stand in Sapporo,Japan

Radon (²²²Rn) and carbon dioxide were monitored simultaneously in soil air under a cool-temperate deciduous stand on the campus of Hokkaido University, Sapporo, Japan. Both ²²²Rn and CO₂ concentrations in soil air varied with atmospheric (soil) temperature in three seasons, except for winter when the temperature in soil air remained constant at 2–3°C at depth of 80 cm. In winter, the gaseous components were influenced by low-pressure region passing through the observation site when the ground surface was covered with snow of ~1 m thickness. Carbon isotopic analyses of CO₂ suggested that CO₂ in soil air may result from mixing of atmospheric air and soil components of different origins, i.e. CO₂ from contemporary soil organic matter and old carbon from deeper source, to varying degrees, depending on seasonal meteorological and thus biological conditions.     

Screening of phthalic acid esters in raw materials,premixes and feed additives

Phthalates are animal carcinogens and may cause death or tissue deformities. Samples of feedstuffs collected in 2005 and 2006 from industrial feed manufacturers in the Czech Republic were analysed for contamination with phthalic acid esters (PAEs), specifically di-2-ethylhexyl phthalate (DEHP) and di-n-butyl phthalate (DBP). Samples of feed additives, premixes and raw materials were collected (year 2005, n = 26). For soybean oil, the total volume of phthalates measured (DBP + DEHP) reached a level of 131.42 mg kg⁻¹; for rapeseed oil, fish meal and animal fats, the levels measured were 15.00, 7.96 and 58.87 mg kg⁻¹, respectively. The lowest level of DBP + DEHP was found in corn (2.03 mg kg⁻¹). Since phthalates were detected, samples of feed additives (n = 28) and raw materials (n = 28) were collected again in 2006. The highest levels of DBP + DEHP were found in raw materials containing fat. Phthalate levels in rapeseed oil samples ranged from 1.38 to 32.40 mg kg⁻¹ DBP + DEHP. For feed additives, contamination levels in vitamins and amino acids ranged from 0.06 to 3.15 and 1.76 to 4.52 mg kg⁻¹ DBP + DEHP, respectively. Here, we show that the levels of PAEs found in cereals such as wheat, barley and corn may be regarded as being alarmingly high, because cereals make up the largest proportion of compound feed of farm animals.     

Food waste in municipal mixed waste produced at household level: empirical evidence from the Czech Republic

Journal of Material Cycles and Waste Management - The problem of food waste is broadly discussed, but empirical data are still missing. The importance of this topic increases as the reporting of...