Role of biochemical energy reserves in the metamorphosis and early juvenile development of the oyster Ostrea chilensis

Metamorphosis in the Chilean oyster Ostrea chilensis was complete 36 h after release of the larvae, when 100% of the individuals showed edge growth of the dissoconch. The size of the larval shell did not change during metamorphosis, although the total dry weight of the larva decreased considerably. During this period, when the gill ciliature was undeveloped and the oyster therefore unable to feed, energy demands were met by biochemical reserves retained from the larval phase. Proteins contributed the largest quantity of energy to the metamorphosing oyster, 69.3% of the total expended, whereas lipids supplied 24.3% and carbohydrates only 6.4%. The process of metamorphosis consumed 64.5% of the energy reserves held by the pediveliger at the time of release. When metamorphosis was complete, growth began and tissue reserves were replenished, protein and carbohydrate accumulating rapidly early in the juvenile stage. Received: 26 December 1997 / Accepted: 8 July 1998     

Ecotoxicological sediment evaluations in marine aquaculture areas of Chile

Given its geographic characteristics, the southern Chilean fjord area is subjected to growing environmental pressure from the development of diverse forms of aquaculture (i.e., fish, algae, shellfish). The sediments accumulate substances as a natural sink, and ecotoxicology assays offer a reliable and robust proxy for sediment quality analyses. This study's objective was to establish a mid-range toxicity base line for the sediments in the region by applying a battery of non-specific ecotoxicological assays. Sediment samples (28) were collected in the channels and fjords studied during the CIMAR-Fiordos 11 cruise (July 2005). The sediments were evaluated using different species endemic to the eastern Pacific as targets: Ampelisca araucana, Tisbe longicornis, Arbacia spatuligera, and Dunaliella tertiolecta. The conditions for each assay were reported previously. Of the four species used as ecotoxicological tools, only D. tertiolecta differed significantly from the control group (negative) in terms of its growth. This difference could be attributed to nutrient enrichment. In general, we concluded that, although local changes occurred in the sediments, the mesoscale magnitude of the ecotoxicological alterations was small. Nonetheless, a surveillance program should be implemented that would allow us to follow-up and analyze the changes that are taking place in the systems on broader scales of time and space.     

Involvement of siderophores in the reduction of metal-induced inhibition of auxin synthesis in Streptomyces spp

Unlike synthetic metal chelators, microbe-assisted phytoremediation provides plants with natural metal-solubilizing chelators which do not constitute a potential source of environmental pollution. Concurrently with microbial chelators, plant growth promotion can be enhanced through bacterially-produced phytohormones. In this work, the simultaneous production of siderophores and auxins by Streptomyces was studied to gain insight for future application in plant growth and phytoremediation in a metal-contaminated soil. Standard auxin and siderophore detection assays indicated that all of the investigated Streptomyces strains can produce these metabolites simultaneously. However, Al(3+), Cd(2+), Cu(2+), Fe(3+) and Ni(2+), or a combination of Fe(3+) and Cd(2+), and Fe(3+) and Ni(2+) affected auxin production negatively, as revealed by spectrophotometry and gas chromatography-mass spectrometry. This effect was more dramatic in a siderophore-deficient mutant. In contrast, except for Fe, all the metals stimulated siderophore production. Mass spectrometry showed that siderophore and auxin-containing supernatants from a representative Streptomyces species contain three different hydroxamate siderophores, revealing the individual binding responses of these siderophores to Cd(2+) and Ni(2+), and thus, showing their auxin-stimulating effects. We conclude that siderophores promote auxin synthesis in the presence of Al(3+), Cd(2+), Cu(2+) and Ni(2+) by chelating these metals. Chelation makes the metals less able to inhibit the synthesis of auxins, and potentially increases the plant growth-promoting effects of auxins, which in turn enhances the phytoremediation potential of plants.     

Mixed models for assessing correlation in the presence of replication

The need to assess correlation in settings where multiple measurements are available on each of the variables of interest often arises in environmental science. However, this topic is not covered in introductory statistics texts. Although several ad hoc approaches can be used, they can easily lead to invalid conclusions and to a difficult choice of an appropriate measure of the correlation. Lam et al. approached this problem by using maximum likelihood estimation in cases where the replicate measurements are linked over time, but the method requires specialized software. We reanalyze the data of Lam et al. using PROC MIXED in SAS and show how to obtain the parameter estimates of interest with just a few lines of code. We then extend Lam et al.'s method to settings where the replicate measurements are not linked. Analysis of the unlinked case is illustrated with data from a study designed to assess correlations between indoor and outdoor measurements of benzene concentration in the air.     

County‐Scale Rainwater Harvesting Feasibility in the United States: Climate,Collection Area,Density, and Reuse Considerations

Roof rainwater harvesting (RWH) has the potential to augment water supplies for urban and suburban uses throughout the United States (U.S.). Studies of the performance of RWH at the building and city scales in the U.S. are available, but a countrywide overview of the potential performance of RWH at the county scale has not been done before. Three approaches were taken: (1) assess the viability of RWH in terms of the rainfall that could be captured in relation to the water demand in each county (excluding agriculture), (2) evaluate the performance of a “typical” domestic RWH system across all counties with metrics related to its ability to supply the potable and nonpotable demand, and (3) evaluate the effect of adding a 50% rainwater reuse component to the analysis. We find RWH could be a viable supplemental water source in the U.S., particularly in counties of the Pacific Northwest, Central, and Eastern regions (percent demand covered >50%). Low population density counties have the potential to meet their annual water needs with RWH, while high‐density counties could only source a small portion (~20%) of their annual demand with RWH. Typical RWH systems in counties in the Central and Eastern U.S. performed better than in Western counties. Adding a reuse component can be a key factor in making RWH attractive in many areas of the country. This work can inform future water infrastructure investment and planning in the U.S.     

Brominated flame retardants in Alburnus alburnus from Cinca River Basin (Spain)

Thirty-two bleak fish (Alburnus alburnus) from three places along the Spanish River Cinca, a tributary of Ebro River, were collected for polybrominated diphenyl ether (PBDE) and hexabromocyclododecane (HBCD) determinations. Sampling sites corresponded to up- and downstream from Monzon, a heavily industrialized town draining to the river. Four different PBDE congeners (BDE-47, -153, -154 and -183) and HBCD were found at levels ranging from not detected (nd) to 573 ng/g wet weight for total PBDEs and from nd to 1643 ng/g wet weight for HBCD. The lowest values were found upstream of the industrialized area. Concentrations in fish were compared with previous data in sediment samples collected at the same sampling sites, and large fish to sediment ratios for these brominated compounds indicate that they are highly bioavailable. Moreover, PBDE and HBCD concentrations are correlated with fish length and weight indicating the bioaccumulation of these contaminants.     

旋风除尘器压力损失的试验研究

分析了旋风除尘器压力损失的成因,通过系统的试验考查了压力损失与进口气流平均速度的关系,探讨了粉尘含量对旋风除尘器压力损失的影响和灰斗的压力分布,总结了排气芯管截面积与入口截面积 A0/Ai之比的大小对除尘器压力损失的影响,从而为旋风除尘器的优化设计及操作性能的改善提供了重要的参考价值。     

Biochemical and genotoxic effects in women exposed to pesticides in Southern Ecuador

Environmental Science and Pollution Research - Toxicity refers to the potential of a substance such as a pesticide to cause damage to the structure or functions of an exposed organism. Pesticides...     

Determination of exposure to lead of subjects from southwestern Poland by human hair analysis

The aim of the present work was to investigate the exposure to lead from various sources by investigation of mineral composition of human scalp hair. The research was carried out on hair sampled from 267 young adults living in Wroc?aw (southwest Poland). The effect of the place of residence, diet, and lifestyle on lead content in hair was examined by inductively coupled plasma optical emission spectrometry (ICP-OES). Lead was determined at the wavelength 220.353 nm. These outcomes were reached by linking the results of lead level in hair with the results of questionnaire survey. The mean lead level in hair of the whole examined population was 2.01?±?2.10 mg kg^?1. Lead can enter the human body mainly by inhalation and gastrointestinal absorption. It was found that consuming cheese, fish, and lettuce caused increased level of lead in hair. On the other hand, drinking of milk, tea, coffee, or lemon resulted in decreased content of lead in hair. Additional source of exposure to lead could be cigarette smoking, distance to the traffic road, painting the walls, amalgam filling. Based on the results, it can be concluded that exposure to lead can occur mainly from eating habits and environmental exposure.     

Newborns and low to moderate prenatal environmental lead exposure: might fathers be the key?

This study is part of the BioMadrid Project, a bio-monitoring study designed to assess pollutants in the environment surrounding children born in the Madrid region. Our aim in this report is to evaluate the association between prenatal lead exposure and fetal development using three biological samples (maternal and paternal blood lead at around 34 weeks of gestation as well as cord blood lead levels), three biomarkers of effect in cord blood peripheral lymphocytes (micronucleus in binucleated cells, nucleoplasmic bridges, and nuclear buds), and different anthropometrical characteristics at birth. Maternal and cord blood lead were not associated with newborn measurements or genotoxicity biomarkers. In contrast, increases in father blood lead were coupled with lower weight (mean difference (MD), ?110.8 g; 95 % confidence intervals (95%CI), ?235.6 to 6.00; p?<?0.10) and shorter abdominal (MD, ?0.81 cm; 95%CI, ?1.64 to 0.00; p?<?0.05) and cephalic (MD, ?0.32 cm; 95%CI, ?0.65 to 0.00; p?<?0.05) circumferences at birth as well as with the presence of nucleoplasmic bridges (odds ratio, 1.03; 95%CI, 1.00 to 1.06; p?<?0.05) and nuclear buds (odds ratio, 1.02; 95%CI, 0.99 to 1.04; p?<?0.10). These associations were mainly confined to female babies, in whom paternal lead was also inversely associated with length. Our results support the hypothesis that paternal lead exposure may be affecting the development of newborns.