Heavy metal concentrations in redeveloping soil of mine spoil under plantations of certain native woody species in dry tropical environment, India

Total concentration of heavy metals(Cd, Cr, Cu, Fe, Pb, Ni, Mn and Zn) was estimated in the redeveloping soil of mine spoil under 5-yr old plantations of four woody species namely: Albizia lebbeck, Albizia procera, Tectona grandis and Dendrocalamus strictus.The data recorded in the present study were compared with other unplanted coal mine spoil colliery, which was around to the study site and adjoining area of dry tropical forest. Among all the heavy metals, the maximum concentration was found for Fe and minimum for Cd.However, among all four species, total concentrations of these heavy metals were recorded maximally in the plantation plots of T. grandis except for Fe, while minimally in A. lebbeck except for Zn, whereas, the maximum concentration of Fe and Zn was in the plantation plots of D. strictus and A. procera. Statistical analysis revealed significant differences due to species for all the heavy metals except Cu.Among four species, A. lebbeck, A. procera and D. strictus showed more efficient for reducing heavy metal concentrations whereas T. grandis was not more effective to reduce heavy metal concentrations in redeveloping soil of mine spoil.     

Microbe-mediated sustainable bio-recovery of gold from low-grade precious solid waste: A microbiological overview

In an era of electronics, recovering the precious metal such as gold from ever increasing piles of electronic-wastes and metal-ion infested soil has become one of the prime concerns for researchers worldwide. Biological mining is an attractive, economical and non-hazardous to recover gold from the low-grade auriferous ore containing waste or soil. This review represents the recent major biological gold retrieval methods used to bio-mine gold. The biomining methods discussed in this review include, bioleaching, bio-oxidation, bio-precipitation, bio-flotation, bio-flocculation, bio-sorption, bio-reduction, bio-electrometallurgical technologies and bioaccumulation. The mechanism of gold biorecovery by microbes is explained in detail to explore its intracellular mechanistic, which help it withstand high concentrations of gold without causing any fatal consequences. Major challenges and future opportunities associated with each method and how they will dictate the fate of gold bio-metallurgy from metal wastes or metal infested soil bioremediation in the coming future are also discussed. With the help of concurrent advancements in high-throughput technologies, the gold bio-exploratory methods will speed up our ways to ensure maximum gold retrieval out of such low-grade ores containing sources, while keeping the gold mining clean and more sustainable.     

Phytomonitoring of urban-industrial pollutants: A new approach

Biological effect monitoring of urban-industrial pollutants was carried out using an air pollution tolerance index (APTI) of plants. For the purpose four leaf parameters — namely, ascorbic acid, total chlorophyll, relative water content and leaf extract pH — were combined together in a formulation signifying the APTI of plants. The index indicated the plant response at the cell membrane and chloroplast levels. SitesP ₁,P ₂ andP ₃ were selected in western parts of Varanasi, India, near the junction of a national highway and a railway track. The control site was selected in the University campus. In the prevailing wind direction from siteP ₁, pollution zones I, II and III were marked. None of the four above mentioned plant parameters indicated a consistent response to pollutants. However, the APTI showed the most sensitive and consistent response of plants to urban-industrial pollutants. The APTI of plants showed a marked gradation as the pollutant load decreased from zone I, II and III to the control site. The APTI can be used as a good indicator of the impact of pollution on plants.     

Dissipation of some organochlorine insecticides in cropped and uncropped soil

Dissipation of four organochlorine insecticides, viz. aldrin, HCH, chlordane and heptachlor was studied in a sandy loam soil with and without crops during a period of 10 cropping seasons. Dissipation of all chemicals followed first-order kinetics (r(2)=0.537 - 0.976) with almost similar persistence in cropped and uncropped soils for all the insecticides. The average half-lives, (t(1/2) values) for total residues of aldrin, HCH, chlordane, and heptachlor in cropped treatments were 80.7, 58.8, 93.2, and 110 days. Their respective values in fallow plots were 78.4, 83.8, 154, and 116 days. None of the parent compounds or their isomers could be detected below the 20 cm depth at the termination of the experiment. Highest residue concentrations were observed in the surface 10 cm layer in fallow plots, but in the deeper (10-20 cm) layer in cropped plots. Analysis of plants and grains showed significant residues of all the chemicals. Degradation of these compounds in cropped and uncropped plots is discussed with regard to their volatilization, microbial degradation, leaching, and plant uptake.     

Enumeration of methanogens with a focus on fluorescence in situ hybridization

Methanogens, the members of domain Archaea are potent contributors in global warming. Being confined to the strict anaerobic environment, their direct cultivation as pure culture is quite difficult. Therefore, a range of culture-independent methods have been developed to investigate their numbers, substrate uptake patterns, and identification in complex microbial communities. Unlike other approaches, fluorescence in situ hybridization (FISH) is not only used for faster quantification and accurate identification but also to reveal the physiological properties and spatiotemporal dynamics of methanogens in their natural environment. Aside from the methodological aspects and application of FISH, this review also focuses on culture-dependent and -independent techniques employed in enumerating methanogens along with associated problems. In addition, the combination of FISH with micro-autoradiography that could also be an important tool in investigating the activities of methanogens is also discussed.     

Modeling soil and plant phosphorus within DSSAT

The crop models in the Decision Support System for Agrotechnology Transfer (DSSAT) have served worldwide as a research tool for improving predictions of relationships between soil and plant nitrogen (N) and crop yield. However, without a phosphorus (P) simulation option, the applicability of the DSSAT crop models in P-deficient environments is limited. In this study, a soil-plant P model integrated to DSSAT was described, and results showing the ability of the model to mimic wide differences in maize responses to P in Ghana are presented as preliminary attempts to testing the model on highly weathered soils. The model simulates P transformations between soil inorganic labile, active and stable pools and soil organic microbial and stable pools. Plant growth is limited by P between two concentration thresholds that are species-specific optimum and minimum concentrations of P defined at different stages of plant growth. Phosphorus stress factors are computed to reduce photosynthesis, dry matter accumulation and dry matter partitioning. Testing on two highly weathered soils from Ghana over a wide range of N and P fertilizer application rates indicated that the P model achieved good predictability skill at one site (Kpeve) with a final grain yield root mean squared error (RMSE) of 535 kg ha⁻¹and a final biomass RMSE of 507 kg ha⁻¹. At the other site (Wa), the RMSE was 474 kg ha⁻¹ for final grain yield and 1675 kg ha⁻¹ for final biomass. A local sensitivity analysis indicated that under P-limiting conditions and no P fertilizer application, crop biomass, grain yield, and P uptake could be increased by over 0.10% due to organic P mineralization resulting from a 1% increase in organic carbon. It was also shown that the modeling philosophy that makes P in a root-free zone unavailable to plants resulted in a better agreement of simulated crop biomass and grain yield with field measurements. Because the complex soil P chemistry makes the availability of P to plants extremely variable, testing under a wider range of agro-ecological conditions is needed to complement the initial evaluation presented here, and extend the use of the DSSAT-P model to other P-deficient environments.     

Impact of osmotic stress on seed germination and seedling growth in black gram (Phaseolus mungo)

An experiment was conducted to see the impact of osmotic stress as it is one of the main cause in various soil and water disorders in agricultural field crops, speciallythe seed germination and seedling growth. The osmotic stress was generated using PEG-6000 and the seed germination, seedling growth were evaluated including the status of pigments i.e. chlorophyll (a, b and total), total carotenoids, pheophytin (a, b and total) and different enzymes like amylase, peroxidase, catalase and superoxide dismutase. The various osmotic potentials generated (-2, -5 and -10 bars) showed significant decrease in germination percentage as at the osmotic potential of -10 bars it was observed 70 in comparison to 90% of control. All the seedling growth parameter also showed inhibition with increase in osmotic potential. Increase in osmotic stress decreased Chlorophyll 'a', while Chlorophyll 'b' was increased in -5 bars while total chlorophyll showed decrease in -5 bars osmotic potential. Total carotenoids and pheophytin (a, b and total) were highly increased in -5 bars and decreased in -10 bars osmotic concentration. Enzymatic activity was found to be decreased in amylase while peroixidase, catalase and SOD were increased at different osmotic gradients in comparison to control. The data observed in the experiment can be helpful to assess the impact of any kind of osmotic stress on plant growth and development in crops.