Non-invasive measurement of the physiological stress response of wild rabbits to the odour of a predator

Summary. Stress has been widely studied in different mammals, but the physiological stress reaction that the odour of a predator could induce in preys has not received much attention. Besides, not all the animals would respond to the same extent to a known stressor. We developed an experimental procedure with eleven na?ve European rabbits (Oryctolagus cuniculus) in order to determine the individual physiological response to the olfactory detection of a potential predator. The rabbits were housed singly in small enclosures with a concrete burrow system and food and water were available ad libitum. The animals followed a control trial, without odour, and an experimental trial where we confronted the rabbits with fox (Vulpes vulpes) odour. Furthermore, another sample of eleven rabbits followed a control procedure subjected to the same housing and handling procedures but without facing the predator odour. In order to assess the physiological response we analysed the concentration of glucocorticoid metabolites in the faeces of the rabbits. Therefore, everyday faecal samples were collected and analysed with an enzyme immunoassay in order to measure the corticosterone metabolites (CM), particularly, those metabolites with a 5α-3β, 11β-diol structure. After validating the assay for wild rabbits, we found that the simulated presence of a predator (fox odour) in the enclosure resulted in an increase in faecal CM concentrations. However, the stressor did not affect all the animals in the same way. We found a general increase in the individual differences. In particular, males experienced a higher increase than females, though the overall response was similar for both sexes. To our knowledge this is one of the first attempts to analyse the assessment of the risk of predation by means of non-invasive methods.     

The effects of social support on nuclear worker responses to the Three Mile Island accident

This study examines the effects of social support on the reactions of nuclear workers at the Three Mile Island plant and a comparison site to stress during the 1979 crisis. Results show broad main effects of social support on stress, strain, and health outcome variables. Moderating effects of support are highly selective and become less extensive as the hypothesized stress process moves from stress to strain to health outcomes. Findings are compared between the two nuclear plants and with other studies conducted under non-crisis conditions.     

Risk assessment of the use of PAS100 green composts in sheep and cattle production in Scotland

A generalized quantitative risk assessment for the use of source-segregated green waste (SSGW) compost use in livestock production is presented. This assessment focussed on potential risks associated with a specific product, PAS100 compost that meets the UK publicly available specification 100 and represents the majority of compost available for use in extensive Scottish livestock systems. A hazard screening approach was used to identify all potentially hazardous agents present in compost. A total of 497 potentially hazardous agents were screened, with 147 finally put forward for quantitative risk assessment. Scenarios modelled in the assessment included surface application of compost to grazing land and also incorporation into soil and subsequent uptake by fodder crops. Risk estimates were compared to those associated with six comparator materials, including various sludges, slurries and farm yard manures. Overall, five potentially hazardous agents (PCB28, PCB138, PCB153, 1,2,3,4,6,7,8-HpCDD, Clopyralid) returned a hazard quotient >1 but within margins of uncertainty, indicating that further investigation may be required. Within the limitations of available information, SSGW compost was found to pose less risk to grazing livestock, or the environment, than other commonly-used soil amendments. While this assessment relates to a specific product/standard used in the UK, the methodology could easily be applied to other composts/products/situations. Therefore these results have wider applicability.     

Evaluating exposure of pedestrians to airborne contaminants associated with non-potable water use for pavement cleaning

Climate change and increasing demography press local authorities to look after affordable water resources and replacement of drinking water for city necessities like street and pavement cleaning by more available raw water. Though, the substitution of drinking by non-drinking resources demands the evaluation of sanitary hazards. This article aims therefore to evaluate the contribution of cleaning water to the overall exposure of city dwellers in case of wet pavement cleaning using crossed physical, chemical and biological approaches. The result of tracer experiments with fluorescein show that liquid water content of the cleaning aerosol produced is about 0.24 g m^?3, rending possible a fast estimation of exposure levels. In situ analysis of the aerosol particles indicates a significant increase in particle number concentration and particle diameter, though without change in particle composition. The conventional bacterial analysis using total coliforms as tracer suggests that an important part of the contamination is issued from the pavement. The qPCR results show a more than 20-fold increase of background genome concentration for Escherichia coli and 10-fold increase for Enterococcus but a negligible contribution of the cleaning water. The fluorescence analysis of the cleaning aerosol confirms the above findings identifying pavement surface as the major contributor to aerosol organic load. The physical, chemical and microbiological approaches used make it possible to describe accurately the cleaning bioaerosol and to identify the existence of significantly higher levels of all parameters studied during the wet pavement cleaning. Though, the low level of contamination and the very short time of passage of pedestrian in the zone do not suggest a significant risk for the city dwellers. As the cleaning workers remain much longer in the impacted area, more attention should be paid to their chronic exposure.     

Global change impacts on the Upper Danube Catchment (Central Europe): a study of participatory modeling

Participatory modeling (PM) has become an essential concept in environmental impact assessment and planning in the field of water resources. In this paper, we focus on the use of PM to support the development of the integrated regional modeling system DANUBIA as a scientific concept to analyze the previously unknown impacts of global change, i.e., the combined effects of climate, demographic, economic, social and ecological change, on the Upper Danube Catchment (Germany). We use this case study to examine the specific conditions for PM in the field of complex integrated models on a regional scale. We describe the stepwise PM process and discuss the respective results, focusing on (1) the stakeholder dialogue’s contribution in supporting the development of new, complex modeling systems, particularly on a regional scale, (2) conditions of stakeholder involvement in issues related to the distant future, such as climate change impacts on regional water availability, and (3) limitations of PM and scientists’ motivation to carry out participatory research at all. We conclude that the PM process was not entirely successful in improving the scientific quality and practical applicability of the developed models because the process goals were manifold and overambitious, and the definition of the problem of “global change impacts on a regional scale” was too weak and uncertain to allow for a clear common objective of modelers and stakeholders. We claim that there is a lack of incentives for scientists, particularly natural scientists, to commit to PM activities.     

The sensitivity of current and future forest managers to climate-induced changes in ecological processes

Climate vulnerability of managed forest ecosystems is not only determined by ecological processes but also influenced by the adaptive capacity of forest managers. To better understand adaptive behaviour, we conducted a questionnaire study among current and future forest managers (i.e. active managers and forestry students) in Austria. We found widespread belief in climate change (94.7 % of respondents), and no significant difference between current and future managers. Based on intended responses to climate-induced ecosystem changes, we distinguished four groups: highly sensitive managers (27.7 %), those mainly sensitive to changes in growth and regeneration processes (46.7 %), managers primarily sensitive to regeneration changes (11.2 %), and insensitive managers (14.4 %). Experiences and beliefs with regard to disturbance-related tree mortality were found to particularly influence a manager’s sensitivity to climate change. Our findings underline the importance of the social dimension of climate change adaptation, and suggest potentially strong adaptive feedbacks between ecosystems and their managers.

Electronic supplementary material

The online version of this article (doi:10.1007/s13280-015-0737-6) contains supplementary material, which is available to authorized users.     

Methyl chloride emissions from halophyte leaf litter: dependence on temperature and chloride content

Methyl chloride (CH₃Cl) is the most abundant natural chlorine containing compound in the atmosphere, and responsible for a significant fraction of stratospheric ozone destruction. Understanding the global CH₃Cl budget is therefore of great importance. However, the strength of the individual sources and sinks is still uncertain. Leaf litter is a potentially important source of methyl chloride, but factors controlling the emissions are unclear. This study investigated CH₃Cl emissions from leaf litter of twelve halophyte species. The emissions were not due to biological activity, and emission rates varied between halophyte species up to two orders of magnitude. For all species, the CH₃Cl emission rates increased with temperature following the Arrhenius relation. Activation energies were similar for all investigated plant species, indicating that even though emissions vary largely between plant species, their response to changing temperatures is similar. The chloride and methoxyl group contents of the leaf litter samples were determined, but those parameters were not significantly correlated to the CH₃Cl emission rate.     

Fugacity modelling to predict the distribution of organic contaminants in the soil:oil matrix of constructed biopiles

Level I and II fugacity approaches were used to model the environmental distribution of benzene, anthracene, phenanthrene, 1-methylphenanthrene and benzo[a]pyrene in a four phase biopile system, accounting for air, water, mineral soil and non-aqueous phase liquid (oil) phase. The non-aqueous phase liquid (NAPL) and soil phases were the dominant partition media for the contaminants in each biopile and the contaminants differed markedly in their individual fugacities. Comparison of three soils with different percentage of organic carbon (% org C) showed that the % org C influenced contaminant partitioning behaviour. While benzene showed an aqueous concentration worthy of note for leachate control during biopiling, other organic chemicals showed that insignificant amount of chemicals leached into the water, greatly reducing the potential extent of groundwater contamination. Level II fugacity model showed that degradation was the dominant removal process except for benzene. In all three biopile systems, the rate of degradation of benzo(a)pyrene was low, requiring more than 12 years for soil concentrations from a spill of about 25 kg (100 mol) to be reduced to a concentration of 0.001 microgg(-1). The removal time of 1-methylphenanthrene and either anthracene or phenanthrene was about 1 and 3 years, respectively. In contrast, benzene showed the highest degradation rate and was removed after 136 days in all biopile systems. Overall, this study confirms the association of risk critical contaminants with the residual saturation in treated soils and reinforces the importance of accounting for the partitioning behaviour of both NAPL and soil phases during the risk assessment of oil-contaminated sites.