城市污水处理厂活性污泥中氨氧化菌群落结构研究

为了解析污水处理厂中氨氧化菌(AOB)的群落结构,以及处理工艺、规模等参数对AOB群落结构的影响,采用针对AOB功能基因氨单加氧酶(amoA)的末端限制性片段长度多态性技术(T-RFLP)、克隆测序等方法,研究了北京市6个污水处理厂的9个污水处理系统中AOB的群落结构.T-RFLP指纹图谱表明不同污水处理系统中AOB的群落结构有所不同,主要的末端限制性片段(T-RF)为354、491和291 bp.T-RFLP指纹图谱及聚类分析表明,污水处理工艺对系统中AOB的群落结构影响较小,而处理规模对AOB的群落结构有一定的影响.对功能基因amoA的系统发育分析表明,污水处理系统中优势AOB均为Nitrosomonasspp.,而非Nitrosospiraspp..这可能是Nitrosomonasspp.的最大比增长速率(μmax)较高,使其更容易成为活性污泥系统中的优势AOB.     

Unified methodology for site-characterization and sampling of highway runoff

Hydrology, roadway traffic conditions, and atmospheric deposition are three essential data categories for the planning and implementation of highway-runoff monitoring and characterization programs. Causal variables pertaining to each data category could be site specific but have been shown to correlate with runoff pollutant loads. These data categories were combined to derive statistical relationships for characterization and prioritization of the respective pollutant loads at highway runoff sites. Storm runoff data of total suspended solids (TSS), total dissolved solid (TDS), chemical oxygen demand (COD), total Kjeldahl nitrogen (TKN) and total phosphorus (TP) collected from three highway sites in Charlotte, North Carolina, USA, were used to illustrate the development of site-specific highway-runoff pollutant loading models. This unified methodology provides a basis for initial assessment of the pollutant-constituent loads from highway runoff using hydrologic component variables. Improved reliability is achievable when additional traffic and/or atmospheric component variables are incorporated into the basic hydrologic regression model. In addition, operational guidance is suggested for implementing highway-runoff monitoring programs that are subject to sampling and resources constraints.     

Prevalence of sustainable and unsustainable use of wild species inferred from the IUCN Red List of Threatened Species

Unsustainable exploitation of wild species represents a serious threat to biodiversity and to the livelihoods of local communities and Indigenous peoples. However, managed, sustainable use has the potential to forestall extinctions, aid recovery, and meet human needs. We analyzed species-level data for 30,923 species from 13 taxonomic groups on the International Union for Conservation of Nature Red List of Threatened Species to investigate patterns of intentional biological resource use. Forty percent of species (10,098 of 25,009 species from 10 data-sufficient taxonomic groups) were used. The main purposes of use were pets, display animals, horticulture, and human consumption. Intentional use is currently contributing to elevated extinction risk for 28–29% of threatened or near threatened (NT) species (2752–2848 of 9753 species). Intentional use also affected 16% of all species used (1597–1631 of 10,098). However, 72% of used species (7291 of 10,098) were least concern, of which nearly half (3469) also had stable or improving population trends. The remainder were not documented as threatened by biological resource use, including at least 172 threatened or NT species with stable or improving populations. About one-third of species that had use documented as a threat had no targeted species management actions to directly address this threat. To improve use-related red-list data, we suggest small amendments to the relevant classification schemes and required supporting documentation. Our findings on the prevalence of sustainable and unsustainable use, and variation across taxa, can inform international policy making, including the Intergovernmental Science-Policy Platform on Biodiversity and Ecosystem Services, the Convention on Biological Diversity, and the Convention on International Trade in Endangered Species.     

The scope and severity of white-nose syndrome on hibernating bats in North America

Assessing the scope and severity of threats is necessary for evaluating impacts on populations to inform conservation planning. Quantitative threat assessment often requires monitoring programs that provide reliable data over relevant spatial and temporal scales, yet such programs can be difficult to justify until there is an apparent stressor. Leveraging efforts of wildlife management agencies to record winter counts of hibernating bats, we collated data for 5 species from over 200 sites across 27 U.S. states and 2 Canadian provinces from 1995 to 2018 to determine the impact of white-nose syndrome (WNS), a deadly disease of hibernating bats. We estimated declines of winter counts of bat colonies at sites where the invasive fungus that causes WNS (Pseudogymnoascus destructans) had been detected to assess the threat impact of WNS. Three species undergoing species status assessment by the U.S. Fish and Wildlife Service (Myotis septentrionalis, Myotis lucifugus, and Perimyotis subflavus) declined by more than 90%, which warrants classifying the severity of the WNS threat as extreme based on criteria used by NatureServe. The scope of the WNS threat as defined by NatureServe criteria was large (36% of Myotis lucifugus range) to pervasive (79% of Myotis septentrionalis range) for these species. Declines for 2 other species (Myotis sodalis and Eptesicus fuscus) were less severe but still qualified as moderate to serious based on NatureServe criteria. Data-sharing across jurisdictions provided a comprehensive evaluation of scope and severity of the threat of WNS and indicated regional differences that can inform response efforts at international, national, and state or provincial jurisdictions. We assessed the threat impact of an emerging infectious disease by uniting monitoring efforts across jurisdictional boundaries and demonstrated the importance of coordinated monitoring programs, such as the North American Bat Monitoring Program (NABat), for data-driven conservation assessments and planning.     

US National Park Buffer Zones: Historical, Scientific, Social, and Legal Aspects

/ This review will trace the evolution of beyond boundary/buffer zone thinking and policy responses in the US National Park Service (NPS); address buffer zone science, benefits, and limitations; examine pertinent legal and social concerns; highlight some agency attempts to create buffer zone-like areas; and propose highlights of a protected area strategy, with buffer zones and corridors as one component. Some findings follow. The need to expand national parks to accommodate large ungulate movement began in the late 1800s, but the recognition that such land was also needed to thwart human impacts such as poaching surfaced in the 1930s. External park buffer zone recommendations by 1930s park scientists were not implemented, and other related adopted policy forgotten, supporting the belief that great insight can be discovered in forgotten institutional history. Buffer zones can remedy some impacts but not others, but their benefits are multiple and underappreciated. The science of buffer zones is very immature and deserves more attention. A present primary obstacle to creating park buffer zones and connecting corridors is a social climate opposing federal initiatives that may intrude on the rights of private landowners. Some proactive NPS bufferlike activity examples are reviewed, but there were none where permanent, complete, effective nonlegislated park buffer zones, derived from nonfederal property, circumscribed large natural area parks. The need for buffer zones and corridors may be a symptom of inadequate regional planning. Options to create buffer zones from private and federal land are outlined. A comprehensive, overall protected area strategy must include more than just buffer zones, with highlights provided. Because optimal regional planning for US national parks is now thwarted by land-use politics, American society must soon decide what is most crucial to future well-being. KEY WORDS: Buffer zone; Reserve; Boundary; Policy; Planning     

EFFECTWENESS OF INTEGRATED STORMWATER MANAGEMENT IN A PORTLAND,OREGON, WATERSHED1

ABSTRACT: The City of Portland's stormwater management program, winner of EPA's Environmental Excellence Award for 1996, is committed to partnership-based, cost-effective, “green” approaches to healthy neighborhoods and water quality. The stormwater program encourages innovative, non-structural pollution reduction techniques like native landscaping, stormwater pollution reduction bioswales and ponds, and public involvement and education. Effectiveness of stormwater best management practices (BMPs) has been difficult to determine on a citywide basis. Recognizing this problem, the City of Portland launched the Parkrose Pilot Project in 1994 to test the effectiveness of a wide range of BMPs in a small watershed in north Portland, the Parkrose catchment, and monitor the results prior to citywide implementation. This catchment was selected because of its small size (144 acres), its representative mix of land uses, and an extensive record of water quality monitoring data. This paper examines the City's strategy in selecting the Parkrose study area as a pilot watershed, the BMPs chosen for use in the watershed, and the results of the program to date. Final success of the Parkrose project will be gauged by the attainment of measurable pollution reduction within the catchment while providing opportunities for meaningful participation by the local community in achieving water quality. Involvement by private citizens in the community is crucial to the success of the project and to ensure compliance with the federal mandate to reduce pollutants to the maximum extent practicable.     

A tree and climate assessment tool for modelling ecosystem response to climate change

Understanding how vulnerable forest ecosystems are to climate change is a key requirement if sustainable forest management is to be achieved. Modelling the response of species in their regeneration niche to phenological and biophysical processes that are directly influenced by climate is one method for achieving this understanding. A model was developed to investigate species resilience and vulnerability to climate change within its fundamental-regeneration niche. The utility of the developed model, tree and climate assessment (TACA), was tested within the interior Douglas-fir ecosystem in south-central British Columbia. TACA modelled the current potential tree species composition of the ecosystem with high accuracy and modelled significant responses amongst tree species to climate change. The response of individual species suggests that the studied ecosystem could transition to a new ecosystem over the next 100 years. TACA showed that it can be an effective tool for identifying species resilience and vulnerability to changes in climate within the most sensitive stage of development, the regeneration phase. The TACA model was able to identify the degree of change in phenological and biophysical variables that control tree establishment, growth and persistence. The response to changes in one or more of these variables resulted in changes in the climatic suitability of the ecosystem for species and enabled a measure of vulnerability to be quantified. TACA could be useful to forest managers as a decision support tool for adaptation actions and by researchers interested in modelling stand dynamics under climate change.     

Linking life history to landscape for threatened species conservation in a multiuse region

Landscape-scale conservation that considers metapopulation dynamics will be essential for preventing declines of species facing multiple threats to their survival. Toward this end, we developed a novel approach that combines occurrence records, spatial–environmental data, and genetic information to model habitat, connectivity, and patterns of genetic structure and link spatial attributes to underlying ecological mechanisms. Using the threatened northern quoll (Dasyurus hallucatus) as a case study, we applied this approach to address the need for conservation decision-making tools that promote resilient metapopulations of this threatened species in the Pilbara, Western Australia, a multiuse landscape that is a hotspot for biodiversity and mining. Habitat and connectivity were predicted by different landscape characteristics. Whereas habitat suitability was overwhelmingly driven by terrain ruggedness, dispersal was facilitated by proximity to watercourses. Although there is limited evidence for major physical barriers in the Pilbara, areas with high silt and clay content (i.e., alluvial and hardpan plains) showed high resistance to dispersal. Climate subtlety shaped distributions and patterns of genetic turnover, suggesting the potential for local adaptation. By understanding these spatial–environmental associations and linking them to life-history and metapopulation dynamics, we highlight opportunities to provide targeted species management. To support this, we have created habitat, connectivity, and genetic uniqueness maps for conservation decision-making in the region. These tools have the potential to provide a more holistic approach to conservation in multiuse landscapes globally.     

Creating conservation strategies with value-focused thinking

Biodiversity and human well-being strategies are only as good as the set of ideas people think about. We evaluated value-focused thinking (VFT), a framework that emphasizes creating objectives and strategies that are responsive to the objectives. We performed a proof-of-concept study of VFT with 6 conservation planning teams at a global conservation organization. We developed a package of materials related to VFT, including meeting–session agendas, a virtual facilitation template, facilitator's guide, and evaluation questionnaires. We used these materials to test whether VFT applied in a group setting resulted in high-quality conservation strategies and participant satisfaction and whether our materials were scalable, meaning that someone newly trained in VFT could facilitate planning meetings that resulted in high-quality strategies and participant satisfaction, as compared with an experienced VFT facilitator. Net response indicated positive compelling, feasible, creative, and representative ratings for the conservation strategies per team. Participants indicated satisfaction overall, although satisfaction was greater for objectives than for strategies. Among the participants with previous conservation planning experience, all were at least as satisfied with their VFT strategies compared with previously developed strategies, and none were less satisfied (p = 0.001). Changes in participant satisfaction were not related to facilitator type (experienced or inexperienced with VFT) (p > 0.10). Some participants had a preconceived sense of shared understanding of important values and interests before participating in the study, which VFT strengthened. Our results highlight the advantages of structuring the development and evaluation of conservation planning frameworks around VFT.