The Frequency of Micronuclei among Newborns from Kragujevac, Central Serbia, after NATO Bombing in the Spring of 1999

Kragujevac, as an important industrial and economic center of the region, is now placed on the top of the list of environmentally endangered cities in our country. The aim of this biomonitoring study was to evaluate cytogenetic damage in a sample of newborns from Kragujevac after contamination of the environment entailed by the intensive bombing of the industrial zone of this city in the spring of 1999. The frequency of micronuclei (MN) in peripheral blood lymphocytes in a total of 61 phenotypically healthy newborns was analyzed before and after the NATO bombing. Analysis of micronuclei has been performed using the cytokinesis-block technique (CB MN test). Average MN frequency in lymphocytes of newborns before the bombing (N = 25) was 5.77 ± 0.85/1000 analyzed cells. After the bombing (N = 36), the average frequency of MN increased by a factor of 1.4 (8.11 ± 0.85), compared to the control frequency before the bombing. Statistical difference (p < 0.05) was established by Student's t-test. Our data suggest that such changes in genetic material were a direct consequence of contamination of the living environment.     

Mitigating Greenhouse Gas Emissions from Rice-Wheat Cropping Systems in Asia

The rice-wheat belt comprises nearly 24–27 million ha in South and East Asia. Rice is generally grown in flooded fields whereas the ensuing wheat crop requires well-drained soil conditions. Consequently, both crops differ markedly in nature and intensity of greenhouse gas (GHG) fluxes, namely emission of (1) methane (CH₄) and (2) nitrous oxide (N_2O) as well as the sequestration of (3) carbon dioxide. Wetland rice emits large quantities of CH₄; strategies to CH₄ emissions include proper management of organic inputs, temporary (mid-season) field drainage and direct seeding. As for the wheat crop, the major GHG is N_2O that is emitted in short-term pulses after fertilization, heavy rainfall and irrigation events. However, N_2O is also emitted in larger quantities during fallow periods and during the rice crop as long as episodic irrigation or rainfall result in aerobic-anaerobic cycles. Wetland rice ensures a relatively high content of soil organic matter in the rice-wheat system as compared to permanent upland conditions. In terms of global warming potential, baseline emissions of the rice-wheat system primarily depend on the management practices during the rice crop while emissions from the wheat crop remain less sensitive to different management practices. The antagonism between CH₄ and N_2O emissions is a major impediment for devising effective mitigation strategies in rice-wheat system - measures to reduce the emission of one GHG often intensify the emission of the other GHG.     

Scenarios for integrated river catchment and coastal zone management

The European Water Framework Directive requires EU Member States to introduce water quality objectives for all water bodies, including coastal waters. Measures will have to be introduced if these objectives are not met, given predictions based on current trends. In this context, the estimation of future fluxes of nutrients and contaminants in the catchment, and the evaluation of policies to improve water quality in coastal zones are an essential part of river basin management plans. This paper investigates the use of scenarios for integrated catchment/coastal zone management in the Humber Estuary in the U.K. The context of this ongoing research is a European research project which aims to assist the implementation of integrated catchment and coastal zone management by analysing the response of the coastal sea to changes in fluxes of nutrients and contaminants from the catchments. The example of the Humber illustrates how scenarios focusing on water quality improvement can provide a useful tool to investigate future fluxes and evaluate policy options for a more integrated coastal/catchment management strategy.     

Phosphate solubilizing actinomycetes in the estuarine environment: an inventory.

Sediment samples were collected from different stations of the Vellar estuary for isolation of total actinomycetes and phosphate solubilizing actinomycetes. Phosphatase activity in the sediments was also investigated Consistently a higher number of actinomycetes, phosphate solubilizing actinomycetes and phosphatase activity were recorded from the clay sediments than the sandy sediments at all the stations. In all, 7 strains showed positive phosphatase activity. Among them, one strain PS-3 exhibited good activity and was further investigated for optimum phosphorus solubilization at different pH (6, 6.5, 7, 7.5 and 8) and incubation (1st day to 20th day) periods. The solubilizing activity was maximum at the pH 7 and an incubation period of 13 days was required for an appreciable quantity of phosphorus to be leached into the medium. Based on the chemotaxonomical and conventional methods of identification, the strain PS-3 has been tentatively identified as Streptomyces galbus. The present study indicates that phosphatase enzyme and S. galbus along with other actinomycetes species would play a major role in solubilizing the phosphate in the estuarine ecosystem and increasing the soluble phosphate concentration thereby enhancing the productivity     

Sample preparation for GC analysis of selected pesticides in surface water.

A new isolation procedure for the determination of nitrogen/phosphorous containing pesticides and organochlorine pesticides in water was tested, and statistical evaluation of the recoveries was performed. The procedure, designed specifically for the analysis of semi-volatile compounds in water containing Suspended Particulate Matter (SPM), utilizes a specially designed filtration vessel coupled directly to an SPE cartridge. The studies were based on surface water samples (from the Vistula River) spiked with pesticides. SPM separation and analyte isolation/concentration were carried out in a special filtration vessel. Pesticides were sorbed both on the SPE cartridge and on the suspended matter. The cartridge and the filter with the suspended matter were extracted separately with a solvent, which enabled the determination of analyte distribution between the two. For organochlorine pesticides, between 3 and 60% of the initial amount was found on the filter, while the recoveries in the filtrate ranged from 30 to 98%. Total recoveries of organochlorine pesticides from surface water samples spiked with pesticides using the method described were high, ranging from 90 to 101%. The amounts of nitrogen/phosphorus containing pesticides recovered from the filter were lower than 2%, while those from the filtrate ranged from 69 to 92%. Total recoveries of nitrogen/phosphorus containing pesticides from surface water samples were high, ranging from 71 to 92%.     

Earthquake Planning for Government Continuity

/ The problem of assuring government operational continuity following earthquakes has been given little research attention. Recent earthquake experience has documented that government organizations without a public safety mission do incur damaged facilities and routinely see increases in public demands following an earthquake. Impediments to service delivery associated with such dam-ages can be minimized if agencies address earthquake plan elements likely to enhance postimpact functioning, including: the potential to relocate operations, protection for the workplace, possession of an organizational inventory, emergency instructions for employees, the ability to use volunteers, and communication capacity. Factors associated with the adoption of these plan elements were studied in one county government and its municipal county seat in the southwestern United States. A census of departments within these jurisdictions was asked to complete a questionnaire reporting the level of planning activity relative to each of these plan elements. It was found that the overall level of preparedness was low, but statistically significantly related to agency size, perceived risk, and information seeking. The implications of these findings underscore the potential for disruption to government service delivery and permit the identification of potential avenues for increasing levels of preparedness.KEY WORDS: Emergency planning; Earthquakes; Government preparedness