New technique using galactose-specific lectin for isolation of fetal cells from maternal blood

To isolate fetal cells from maternal blood, we developed a new method based on galactose-bearing conjugation. Nucleated red blood cells (NRBCs), which highly express galactose on their surface, were selectively attached to a substrate coated with a galactose-containing polymer via soybean agglutinin (SBA), a galactose-specific lectin. Cord blood samples were used to evaluate enrichment efficacy of NRBCs by this method. Blood samples were obtained from 131 pregnant women between 6 and 27 gestational weeks. After preliminary condensation of fetal cells by Ficoll gradient centrifugation, NRBCs were enriched using galactose-positive selection by adjusting SBA concentration. We isolated one to severalhundred NRBCs (mean±SD, 7.8±8.5) in 2.3 ml of peripheral blood samples from 96% of pregnantwomen. The isolated NRBCs were analyzed by a Y-chromosome FISH probe in eight cases carrying male fetuses. Y-signals were detected in all eight cases and more than half of the NRBCs wereoffetal origin. The study demonstrates that our new method using galactose-specific lectin provides effective enrichment of fetal NRBCs allowing non-invasive prenatal diagnosis. Copyright © 2002 John Wiley & Sons, Ltd.     

Aryl hydrocarbon receptor activities of hydroxylated polycyclic aromatic hydrocarbons in recombinant yeast cells

Hydroxylated polycyclic aromatic hydrocarbons (OH-PAH) with less than four rings are frequently found in the environment, whereas the toxicities associated with these compounds remain unclear. In this study, aryl hydrocarbon receptor (AhR)–ligand binding activities of OH-PAH were investigated by using a recombinant yeast assay system. The majority of the OH-PAH tested showed AhR–ligand binding activities, especially, when the hydroxylated derivatives of naphthalene were incubated with recombinant yeast. The structure–activity relationship between AhR activity and molecular weight or the octanol–water partition coefficient value of OH-PAH displayed significant correlations. These findings indicate that the site and number of hydroxy-groups substituted on PAH skeleton apparently influenced the AhR – ligand binding activity in the recombinant yeast assay.     

Reality and risk of contact-type head injuries related to bicycle-mounted child seats

Objective

The authors have treated numerous children who have been injured by falling from bicycle-mounted child seats. Despite the greatly increased use of such seats, the understanding of their risk and the importance of helmet use remains alarmingly poor. The objective of this study was to confirm the risk of bicycle-mounted child seats and to evaluate the efficacy of helmets, seat belts, and back seat height in terms of preventing or mitigating contact-type head impacts that occur in falls from bicycle-mounted child seats.

Materials and methods

Biometrical dummy tests were performed to examine contact-type head injuries in falls from stationary bicycles. A bicycle with an anthropometric test dummy placed in a bicycle-mounted child seat was tipped over. Each test was repeated three times and three-dimensional acceleration was measured using accelerometer. Head Injury Criteria (HIC) were calculated and the respective influences of a helmet, a seat belt, and increased height of the back of the seat on such impacts were evaluated.

Results

Only helmets unequivocally lowered maximal acceleration and/or HIC values with statistical significance. The seat belt lowered HIC values as long as it was used with the high-back seat. Only when the dummy wore a helmet sitting in a high-back seat did the HIC show less than the threshold of 570 for three-year-old children. The HIC showed the lowest score of 161.5 when the dummy wore both a helmet and a seat belt sitting in a high-back seat.

Conclusions

Riders in bicycle-mounted child seats definitely have higher risks of contact-type head injuries. In transporting a child on a bicycle-mounted child seat, parents must use both a child-bicycle helmet and a high-back child seat at least; a seat belt is highly recommended as long as it is used with the other safety devices.

Impact on Industry

The bicycle-mounted child seat should have a high enough back and an appropriate seat belt to protect the head of the child from a contact-type injury.     

Environmental assessment and accounting for the waste disposal stream in Bangkok,Thailand

It is very important that waste should be controlled and appropriately handled in a waste disposal stream, considering its impact on the environment. In this research, the LCA-EA model was applied to the current waste disposal stream of the BMR as well as other waste disposal stream scenarios, so that treatment cost, environmental load and environmental cost were assessed quantatively. The results of this study showed that in the current waste diposal stream in Bangkok, there were large contributions from carbon dioxide and methane to the greenhouse gas emissions from the stream. The study was able to quantify the reductions in environmental load associated with various waste disposal stream scenarios, using the baseline scenario as a standard.     

Challenges to the implementation of a sustainable development certification programme for the minerals and metals industry

Private, voluntary eco-labelling and certification programmes are being developed for a number of industries to promote and verify higher environmental, economic and social standards. In some cases, these initiatives have developed regardless of whether there is initial, broad support within industry. This article seeks to identify some of the challenges inherent in the implementation of a sustainable development certification programme for the minerals and metals industry. The article looks for guidance from initiatives that are drawn for the most part from other resource industries, most notably forestry. While the article takes a Canadian perspective, many of the challenges raised in the article are also applicable internationally. The article concludes that stakeholders can learn from other resource industries if a decision is made to develop a certification programme for the minerals and metals industry. Crucial to the long-term success of such a programme will be the establishment of a true partnership among stakeholders throughout the initiative's development. Crown Copyright.     

Vertical distribution of <Emphasis Type="Italic">Todarodes pacificus</Emphasis> (Cephalopoda: Ommastrephidae) paralarvae near the Oki Islands,southwestern Sea of Japan

The diel vertical distribution patterns of Japanese common squid, Todarodes pacificus, paralarvae were examined using a Multiple Opening Closing Net and Environmental Sensing System (MOCNESS) in the southwest Sea of Japan near the Oki Islands (Japan) during five late-autumn surveys in 1998–2002. A total of 1,511 paralarvae ranging in mantle length (ML) from 0.7 to 7.3 mm were collected at 63 of the 68 stations surveyed. Most (84%) were collected above 75 m depth and in the mixed layer. The vertical distribution patterns varied little between day and night. Hatchling-sized (<1.0 mm ML) paralarvae were abundant at 0–25 m depth, and paralarval ML increased with increasing sampling depth. Our results suggest that T. pacificus paralarvae do not exhibit large diel vertical migration patterns, but as they increase in size, paralarvae gradually descend in the water column and the variability in depth increases with ontogeny.     

Differential anti-lipid peroxidative activity of melatonin

Scavenging activities of melatonin, which is a pineal secretory product and functions in circadian biology, and its related compounds against reactive oxygen species such as superoxide anion radical, hydrogen peroxide, hydroxyl radical and singlet oxygen as well as organic peroxide radical (t-BuOO”) were evaluated chemically by using electron spin resonance-spin trap and chemiluminescence methods. Antioxidative activity of the compounds was estimated by IC₅₀ value (µM), 50% inhibiting concentration of a compound against reactive oxygen species formed in each system, and the second-order rate constants (k₂) for the reactions of the compounds and superoxide anion radical or hydroxyl radical. Because melatonin has exhibited the highest scavenging activity against t-BuOO”, the biochemical anti-lipid peroxide radical scavenging activities of melatonin were examined. We found that melatonin exhibits higher anti-lipid peroxidative activity in the rat brain microsomes than in the rat liver microsomal and liposomal systems, suggesting that melatonin may function as a treatment for reactive oxygen species-related diseases of the brain.     

Community-scale analysis of the farmland abandonment occurrence process in the mountain region of Ladakh,India

Farmland abandonment has been a significant issue in mountain regions. Thus, many studies have attempted to uncover its driving factors. Most existing studies take a large- or meso-scale view of mountain regions, and there are a few studies that provide community-level analysis. Therefore, it is unclear how ecological and sociopolitical factors are actually combined to influence farmland abandonment at a community level. Thus, we conducted a field survey in the mountain village of Ladakh, northern India, beginning in 2009 and tried to understand the farmland abandonment occurrence process from the viewpoint of traditional altitudinal models of land use. Using data collected during the survey, a relationship between altitude and farmland abandonment was established. However, this relationship was complex, combining traditional forms of land use system in the village. The altitudinal model of land use was very common in mountain regions; thus, discussions of current land use issues should be based on an understanding of such traditional altitudinal systems.     

Assessing local-scale inclusive wealth: a case study of Sado Island,Japan

Present trends of urbanization are accompanied by increasing demographic and economic shrinkage of rural regions. In countries such as Japan, these rural regions trail behind metropolitan counterparts according to GDP, the conventional measure used to guide governmental policies. Yet, past research suggests that these regions may be undervalued. Further, the Inclusive Wealth Index (IWI), largely only used at the national level, may be able to capture aspects previously missed. As such, our study attempts to highlight the wealth of rural regions by comparing the inclusive wealth of Sado Island and Japan between 1990 and 2014. Minor methodological modifications were made according to data availability at the local level and to improve the accuracy of human capital estimations. Results captured the ongoing shrinkage of Sado and demonstrate the distinct potential of the IWI as a stock measure. Sado’s per capita wealth was about 10% lower than the national averages, but its natural capital was about threefold national averages. Supplementary estimations of the natural capital of fisheries and cultivated forests suggest that inclusion of additional factors in the evaluation would further increase the relative valuation of rural regions. We discuss implications of our estimations for wellbeing, and conclude with a critical appraisal of the IWI calculation towards policy implementation of the index.