Deer predation on leaf miners via leaf abscission

The evergreen oak Quercus gilva Blume sheds leaves containing mines of the leaf miner Stigmella sp. (Lepidoptera: Nepticulidae) earlier than leaves with no mines in early spring in Nara, central Japan. The eclosion rates of the leaf miner in abscised and retained leaves were compared in the laboratory to clarify the effects of leaf abscission on leaf miner survival in the absence of deer. The leaf miner eclosed successfully from both fallen leaves and leaves retained on trees. However, sika deer (Cervus nippon centralis Kishida) feed on the fallen mined leaves. Field observations showed that deer consume many fallen leaves under Q. gilva trees, suggesting considerable mortality of leaf miners due to deer predation via leaf abscission. This is a previously unreported relationship between a leaf miner and a mammalian herbivore via leaf abscission.     

Determination of dioxin concentrations in fish and seafood samples using a highly sensitive reporter cell line, DR-EcoScreen cells

There is a strong need for the development of relatively rapid and low-cost bioassays for the determination of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs), and dioxin-like polychlorinated biphenyls (dl-PCBs) in environmental and food samples. In this study, we applied a reporter gene assay using DR-EcoScreen cells (DR-cell assay), which is highly sensitive to dioxins, to the determination of PCDD/Fs and dl-PCBs in fish and seafood samples. The PCDD/Fs and dl-PCBs were extracted from homogenated samples (10 g) of 30 fish and shellfish, purified by clean-up procedure using a multilayered silica gel column and an alumina column, and applied to DR-cell assay. Interestingly, the bioanalytical equivalent (BEQ) values obtained from the DR-cell assay [<0.1 ∼ 5.4 pg BEQ g⁻¹ wet weight (ww)] were closely correlated with the toxicity equivalent (TEQ) values from conventional high-resolution gas chromatography/high-resolution mass spectrometry (HRGC-HRMS) analysis (r² = 0.912), and the slope of regression line was 0.913. Therefore, we multiplied the BEQ values from the DR-cell assay by a conversion coefficient (1.095, the reciprocal of 0.913) to approximate the TEQ values from the HRGC-HRMS analysis. Furthermore, we used this DR-cell assay to perform a prescreening test of PCDD/Fs and dl-PCBs in 16 fish and seafood samples purchased from a supermarket, revealing that a sample from the fatty flesh of a bluefin tuna exceeded 8 pg TEQ g⁻¹ ww (the European Union-tolerance limit). Taken together, these results suggest that the DR-cell assay might be applicable as a rapid and low-cost prescreening method to determine dioxin levels in fish and seafood samples.     

The effects of soil and Trifolium repens (white clover) on the fate of estrogen

In this study, we investigated the behavior of estrogens in the rhizosphere of white clover (Trifolium repens, clover hereafter) with two different pot tests, using soil and agar as growth media. In a pot test using agar spiked with estrogen, the estrogen concentration in the agar with clover decreased to non-detectable levels within one month, while in the agar without clover, 60% of initially added estrogen remained after one month. The half-lives of estrone (E1) and 17β -estradiol (E2) in the agar with clover were 2.4–3.8 and 13.2 d, respectively. The dissipation of E1 followed first-order rate law, while that of E2 fitted a zero-order reaction, indicating that they had different mechanisms of dissipation from agar. In the soil pot test, the behavior of E1 and E2 was not influenced by clover. An initial rapid decrease in the amount of estrogen extracted by methanol/acetic acid was followed by persistence for 1–3 months, regardless of presence of clover. Moreover, in three weeks E1 and E2 were only partly degraded by microbes extracted from the soil used in the pot test. In this study, abiotic degradation of estrogens and sorption of estrogen to soil, rather than the effects of soil microbes and clover, contributed to the initial rapid dissipation of estrogens in the soil. However, the results of the agar pot test suggested that vegetation such as clover may significantly contribute to removal of estrogens when estrogens in aqueous phase are discharged with surface runoff and preferential flow after heavy rain in agricultural fields, or when present in soils with low estrogen sorptivity.     

Elevated water temperature reduces the acute toxicity of the widely used herbicide diuron to a green alga, Pseudokirchneriella subcapitata

In the actual environment, temperatures fluctuate drastically through season or global warming and are thought to affects risk of pollutants for aquatic biota; however, there is no report about the effect of water temperature on toxicity of widely used herbicide diuron to fresh water microalgae. The present research investigated inhibitory effect of diuron on growth and photosynthetic activity of a green alga Pseudokirchneriella subcapitata at five different temperatures (10, 15, 20, 25, and 30 °C) for 144 h of exposure. As a result, effective diuron concentrations at which a 50 % decrease in algal growth occurred was increased with increasing water temperature ranging from 9.2 to 20.1 μg L^–1 for 72 h and 9.4–28.5 μg L^–1 for 144 h. The photochemical efficiency of photosystem II (F _v/F _m ratio) was significantly reduced at all temperatures by diuron exposure at 32 μg L^–1 after 72 h. Inhibition rates was significantly increased with decreased water temperature (P?<?0.01). Intracellular H₂O₂ levels as an indicator of oxidative stress were also decreased with increasing temperature in both control and diuron treatment groups and were about 2.5 times higher in diuron treatment groups than that of controls (P?<?0.01). Our results suggest water temperatures may affect the toxicokinetics of diuron in freshwater and should therefore be considered in environmental risk assessment.     

Fitness of occupational exposure data to inverse Gaussian distribution

Short-term exposure datasets of 10 workers exposed to organic solvents and daily average exposure datasets of seven workers exposed to cobalt and organic solvents were analyzed in order to clarify whether these exposure data fit an inverse Gaussian distribution. The goodness-of-fit test showed that time-weighted average (TWA) values with averaging times of 30 min, 60 min and 8 hours can be described by an inverse Gaussian distribution, but not those with averaging times of 7.5 min and 15 min.     

Treatment of bleaching wastewater from pulp paper plants in China using enzymes and coagulants

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Identification of odorous compounds in air and gas from painting and printing industries by gas chromatography-mass spectrometry and library search system

A computer-assisted library search system is described consisting of a data base, pre-search and main search. Odorous air and effluent gas from painting and printing industries were analyzed by gas chromatography-mass spectrometry and the library search system. Main components were 2-methylpropanol, three xylenes, toluene, ethyl acetate and butyl acetate.     

Shedding light on a neglected area: a new approach to knowledge creation

Awareness is needed of sustainability issues that are currently neglected but potentially important. A computer-based approach can highlight unconscious and neglected areas because it can structure vast amounts of knowledge. In this article, we propose a methodology to perceive unconscious areas of sustainability with the support of a computer-based approach, which creates a matrix, named the recognized-unrecognized matrix, which highlights both local and globally neglected problems. A case study is conducted to consider the potential contribution of the aviation industry to sustainability issues. We demonstrate that a citation network analysis is an effective methodology to chart the recognized-unrecognized matrix. We highlight issues of water use in the aviation industry by designing an innovative water and air circulation system, which significantly reduces water and fuel consumption on board airplane flights. We also suggest a new approach to integrating both explicit and tacit knowledge to enable knowledge creation.     

Multi-level perspectives with technology readiness measures for aviation innovation

Sustainability science requires the development of a theoretical framework to understand, analyze, and design innovation to solve social, economic, and environmental issues. This paper extends the framework of multi-level perspectives (MLP) by introducing a technology readiness level (TRL), and analyzes the innovation of the advanced turboprop (ATP) engine in the aviation industry, also known as a propfan or an open rotor engine, which is one of the most promising engine innovations expected to mitigate climate change. The concept of TRL was introduced to explain the mechanisms of ATP failure in the late 1980s as well as the transition of the geared turbofan (GTF). In this paper, we discuss why ATP and GTF faced different fates although both were developed under the same landscape in the aviation industry. We also discuss the different roles of the socio-technical regime, such as uneven and dynamic opportunity windows, technological readiness, niche stock, institutional support of export products, and the risk of a ‘launch’ customer, at different TRLs. As illustrated in this paper, MLP with TRL is expected to facilitate future interdisciplinary collaboration between social scientists and engineers, and also transdisciplinary expertise between academia and practitioners by supporting analysis and design of the industry’s transition toward a more environmentally friendly regime as well as its effective management.