Effect of graphene oxide exposure on intestinal Wnt signaling in nematode Caenorhabditis elegans

Exposure to engineered nanomaterials(ENMs), such as graphene oxide(GO), can potentially induce the response of various molecular signaling pathways, which can mediate the protective function or the toxicity induction.Wnt signaling pathway is conserved evolutionarily in organisms.Using Caenorhabditis elegans as an in vivo assay model, we investigated the effect of GO exposure on intestinal Wnt signaling.In the intestine, GO exposure dysregulated Frizzled receptor MOM-5, Disheveled protein DSH-2, GSK-3(a component of APC complex), and two β-catenin proteins(BAR-1 and HMP-2), which mediated the induction of GO toxicity.In GO exposed nematodes, a Hox protein EGL-5 acted as a downstream target of BAR-1, and fatty acid transport ACS-22 acted as a downstream target of HMP-2.Functional analysis on HMP-2 and ACS-22 suggested that the dysregulation of these two proteins provides an important basis for the observed deficit in functional state of intestinal barrier.Our results imply the association of dysregulation in physiological and functional states of intestinal barrier with toxicity induction of GO in organisms.     

Potential of secondary aerosol formation from Chinese gasoline engine exhaust

Light-duty gasoline vehicles have drawn public attention in China due to their significant primary emissions of particulate matter and volatile organic compounds(VOCs). However,little information on secondary aerosol formation from exhaust for Chinese vehicles and fuel conditions is available. In this study, chamber experiments were conducted to quantify the potential of secondary aerosol formation from the exhaust of a port fuel injection gasoline engine. The engine and fuel used are common in the Chinese market, and the fuel satisfies the China V gasoline fuel standard. Substantial secondary aerosol formation was observed during a 4–5 hr simulation, which was estimated to represent more than 10 days of equivalent atmospheric photo-oxidation in Beijing. As a consequence, the extreme case secondary organic aerosol(SOA) production was 426 ± 85 mg/kg-fuel, with high levels of precursors and OH exposure. The low hygroscopicity of the aerosols formed inside the chamber suggests that SOA was the dominant chemical composition. Fourteen percent of SOA measured in the chamber experiments could be explained through the oxidation of speciated single-ring aromatics. Unspeciated precursors, such as intermediate-volatility organic compounds and semi-volatile organic compounds, might be significant for SOA formation from gasoline VOCs. We concluded that reductions of emissions of aerosol precursor gases from vehicles are essential to mediate pollution in China.     

The effects of autophagy on vascular endothelial cells induced by airborne PM2.5

The purpose of this study was to examine the direct toxicity of PM2.5 collected from Beijing on human umbilical vein endothelial cells(HUVEC). A Cell Counting Kit 8(CCK8) assay demonstrated that PM2.5 exposure decreased the proliferation of HUVECs in a dosedependent manner. We also found that PM2.5 exposure induced autophagy in HUVECs, as evidenced by:(1) an increased number of double-membrane vesicles;(2) enhanced conversion and punctuation of the microtubule-associated protein light chain 3(LC3); and(3) decreased levels of the selective autophagy substrate p62 in a time-dependent manner.Furthermore, promoting autophagy in PM2.5-exposed HUVECs with rapamycin increased the cell survival rate, whereas inhibiting autophagy via 3-methyladenine significantly decreased cell survival. These results demonstrate that PM2.5 exposure can induce cytotoxicity and autophagy in HUVECs and that autophagy play a protective role against PM2.5-induced cytotoxicity. The findings of the present study imply a direct toxic effect of PM2.5 on HUVECs and provide novel insight into the mechanism of cardiovascular diseases caused by PM2.5 exposure.     

垃圾填埋场渗出液中有害成分的研究

研究垃圾填埋场渗出液中的有害成分。渗出液样品用适当的方法处理后 ,用气相色谱 -质谱联用方法和原子发射光谱法进行定性分析。然后用原子吸收分光光度法和反相高效液相色谱法定量测定样品中微量的 Cu、Zn、Pb、Cr、Cd、Hg、苯酚、对甲苯酚、萘、蒽     

Removal of high concentrated ammonia nitrogen from landfill leachate by landfilled waste layer

The landfill of municipal solid waste(MSW) could be regarded as denitrification reactor and involved in ammonia nitrogen biological removal process. In this research, the process was applied to municipal solid waste collected in Shanghai, China, which was characterized by high food waste content. The NH4^ removal efficiency in the system of SBR nitrifying reactor followed by fresh and matured landfilled waste layer in series was studied. In the nitrifying reactor, above 90% of NH4^ in leachate was oxidized to NO2^- and NO3^- . Then high concentrated NO2^- and NO3^- were removed in the way of denitrification process in fresh landfilled waste layer. At the same time, degradation of fresh landfilled waste was accelerated. Up to the day 120, 136.5 gC/(kg dry waste) and 17.9 gN/(kg dry waste) were converted from waste layer. It accounted for 50.15 % and 86.89 % of the total carbon and nitrogen content of preliminary fresh waste, which was 4.42 times and 5.17 times higher than that of reference column respectively. After filtering through matured landfilled waste, BOD5 concentration in leachate dropped to below 100 mg/L, which would not affect following nitrification adversely. Because the matured landfilled waste acted as a well methanogenic reactor, 23% of carbon produced accumulatively from fresh landfilled waste degradation was converted into CH4.     

凡口铅锌矿床地球化学特征及成矿作用分析

凡口铅锌矿床是发育于碳酸盐岩建造中的海底热泉喷溢沉积矿床,以“一大二富三集中”的特点和典型的地质地球化学特征吸引着中外矿床地质工作者。常量元素和矿化元素的分布、矿物包裹体特征、稀土元素组成和硫同位素组成反映出该矿床与外生作用有关。铅同位素组成具有壳源和下地壳来源铅的特征,矿物包裹体水氢、氧同位素组成表明,成矿溶液可能与深层建造水有关,后期与大气降水有关。矿区主要矿层经历海底热泉喷溢沉积-成岩的演化。     

污泥综合处理技术系统的可行性分析

通过对污泥处理技术现状与污泥组成的分析，认为降低污泥的含水率是改善污泥可处理性的关键。以机械脱水加工热干燥过程可达到充分降低污泥含水率的目的。以此为主干所形成的污泥综合处理系统经能量与经济平衡分析，发现其能量输出大于输入，经济成本合理，有一定的技术经济可行性。     

广州市实施二氧化硫排污权交易研究

在排污权交易的理论基础上,借鉴美国二氧化硫排放权交易的政策和方法,设计了一套广州市实施二氧化硫排污权交易的框架,旨在为广州市控制削减二氧化硫排放提供一种基于市场的环境管理手段.     

颗粒物质金属形态逐级提取技术问题的探讨

本文综述了国内外对颗粒物质中金属形态逐级提取测定技术方面的研究情况，重点阐述了颗粒物质金属形态分离方法，并对各相态的浸取技术和金属总量提取方法进行了讨论．从而为开展颗粒物质的金属化学形态的分析和研究，提出几点认识．     

厌氧-混凝工艺处理造纸厂终端废水试验研究

本文报告了用厌氧 -混凝工艺处理造纸厂终端废水的实验情况 ,在最佳实验条件下 ,废水从CODcr80 6 . 7m g/ L、BOD52 10 . 5 m g/ L、SS2 15 . 7m g/ L、OD4 650 . 35 0降至 CODcr6 1. 5 m g/ L、BOD52 7. 4m g/ L、SS17.3mg/ L、OD4 650 .0 11。实验结果表明对造纸厂终端废水的治理 ,本工艺是很有实用价值的一种方法