Biochemical biomarkers in environmental studies—lessons learnt from enzymes catalase,glutathione <Emphasis Type="Italic">S</Emphasis>-transferase and cholinesterase in two crustacean species

Background, aim and scope  

For reliable environmental risk assessment of pollutants, knowledge on the effects at different levels of biological organisation is needed. During the early days of biomarker research in environmental studies approximately two decades ago, biochemical biomarkers were considered as the most promising tool for such purposes. Among these, three enzymes have often been studied: catalase (CAT), glutathione S-transferase (GST) and cholinesterase (ChE). However, despite their intensive research, their measurements in invertebrates have not been commonly applied in environmental risk assessment (ERA) or for regulatory purposes.     

Fe(III) promoted LAS (linear alkylbenzenesulfonate) removal from waters

The mechanisms of the interactions between Fe(III) aquacomplexes and surfactants were investigated; three alkylbenzenzsulfonates, two surfactants (octylbenzenesulfonate (OBS) and dodecylbenzenesulfonate (DBS)), and a shorter derivative (ethylbenzenesulfonate (EBS)) were studied. The results with OBS show evidence for three different ways in which Fe(III) interferes with the surfactant: the widely described flocculation process, complexation of Fe(OH)2+ (aquacomplexes) by the surfactant, and a redox reaction. The formation of a weak complex is maximum for a ratio of three between the monomeric aquacomplex [FeOH(H2O)5]2+ and OBS. In the presence of oxygen, an intramolecular redox reaction occurs inside the complex. The interaction between commercial DBS and Fe(III) was also investigated. Immediate precipitation occurred, mainly involving derivatives of higher molecular weights that are contained in the DBS samples. The constituents with the shortest alkyl chain were not affected by the presence of Fe(III) as it was also observed with EBS.     

Summary of 25 Years of Xylem Tracheids and Sewage/Sludge in the Sea

The Rapid Sewage/Sludge Indicator Technique (RSIT), developed during the early 1970's, responded to critical needs within the Bureau of Water Pollution Control. It enabled quick detection of contamination in ocean water and bottom sediments during sewage-related emergencies and routine monitoring. RSIT became an invaluable tool for resource management, in determining where and to what extent marine environments are impacted, and when immediate information is necessary for public health decisions.

The RSIT utilizes microscopic examination for terrestrial plant vascular tissue, especially the xylem tracheid elements, as identifiers of sewage/sludge. Not normally found in ocean waters, this material, in toilet paper and the roughage of the human diet which passes through the digestive system intact, is an indicator of human faecal contamination. Minimal training and common laboratory materials allow gross screening in five minutes. New studies by the United States Environmental Protection Agency (USEPA), United States Geologic Survey (USGS) and National Oceanic and Atmospheric Administration (NOAA), most recently at the 106-mile dump site off the New York Bight, revalidated the technique as a rapid, inexpensive, easy to use, semiquantitative indicator both for water column and sediment samples.     

Structure characterization and thermal stabilities of the isomers of the brominated flame retardant 1,2-dibromo-4-(1,2-dibromoethyl)cyclohexane

1,2-Dibromo-4-(1,2-dibromoethyl)cyclohexane (TBECH) is used primarily as an additive flame retardant. 1H NMR spectroscopy and an X-ray structure determination have revealed that a technical mixture consists largely of two (of the four possible) diastereomers, rac-(1R,2R)-1,2-dibromo-(4S)-4-((1S)-1,2-dibromoethyl)cyclohexane (alpha-TBECH) and rac-(1R,2R)-1,2-dibromo-(4S)-4-((1R)-1,2-dibromoethyl)cyclohexane (beta-TBECH), in a mole ratio of approximately 1:1. The two other possible isomers, gamma- and delta-TBECH, were not detected in a technical mixture. The TBECH isomers are thermally sensitive and can easily interconvert at temperatures of 125 degrees C. A thermal equilibrium mixture of alpha-, beta-, gamma- and delta-TBECH consists of approximately 33%, 33%, 17% and 17% of these isomers, respectively. Separation of all four TBECH diastereomers, with minimal thermal interconversion of the isomers, was achieved by careful selection of GC-capillary column length and injector temperature. Although technical TBECH does not contain the gamma- and delta-isomers, they may still be relevant environmental contaminants since manufacturing processes utilize thermal processes which may induce their formation.     

Some issues relating to the use of perfluorooctanesulfonate (PFOS) samples as reference standards

Samples of potassium perfluorooctanesulfonate (PFOSK) from three suppliers were analyzed by LC-ESI-MS/MS for purity and by LC-ESI-MS for the percentage of linear isomer present. Our data indicated that the purity ranged from 80% to 98% and the percentages of linear isomer from 67% to 79%. The proportion of branched isomers present in the samples was also estimated using (19)F NMR. These results agreed quite closely with those found by LC-ESI-MS indicating that there is essentially no difference in overall SIM response factor for the branched isomers vs. that of the linear isomer. Several further observations relevant to the use of standards when analyzing for PFOS were encountered during this study. It appears unlikely that matrix effects attributable to the cation (sodium or potassium) present in PFOSNa or PFOSK internal standards is an issue. In seeking potential matrix effects, it was found that the chromatography was improved substantially when the standard was injected as a solution in 80:20 methanol/water rather than 100% methanol. Notably, in concert with the improvement in chromatography, an increase of about 10% in response was observed. In some closely related studies, when (18)O(2) mass-labeled perfluorohexanesulfonate was used as an internal standard, the actual and theoretical concentration ratios matched closely those for related native sulfonates as long as they did not co-elute. However, when they did co-elute, the peak intensities of the native species were enhanced by about 5%, while those of the labeled compound were suppressed by a similar amount. If this effect were not taken into account, the concentration of the native would be inflated by 10%.     

Perceived prosocial impact,perceived situational constraints,and proactive work behavior: Looking at two distinct affective pathways

This paper examines the role of affect as a linking mechanism between experiences at work (perceived prosocial impact and situational constraints) and two distinct components of proactive work behavior (issue identification and implementation). Based on a dual‐tuning perspective, we argue that both positive affect and negative affect can be beneficial for proactive work behavior. Multi‐level path analysis using daily‐survey data from 153 employees showed that perceived prosocial impact predicted positive affect and that situational constraints as a typical hindrance stressor predicted negative affect. Negative affect, in turn, predicted issue identification, and positive affect predicted implementation. Overall, our study suggests that both positive and negative affects can be valuable in the organizational context by contributing to distinct components of proactive behavior. Copyright © 2015 John Wiley & Sons, Ltd.     

Factors influencing the acceptance of nature conservation measures--a qualitative study in Switzerland

Landscapes fulfil a multitude of ecological and social functions. Due to the fact that both traditional and everyday landscapes today face many different threats, regulatory measures have been undertaken in many countries to protect and further sustainable landscape development. They include legislation as well as economic incentives. In recent years, however, it has become clear that just to have a few laws and to spend money on subsidies is not enough. Factors other than legislation are also essential to further the sustainable development of landscapes. One of the basic factors affecting the success or failure of landscape conservation measures is public acceptance of these measures. Our project took this as its starting point. The objective was to determine which conditions and factors influence acceptance positively or negatively. To this end 22 Swiss who are directly affected by nature conservation measures, in particular by mire landscape and dry meadow conservation measures, were interviewed using qualitative interview techniques. It is shown that perception, communication, and possibilities to participate are the most decisive driving factors influencing the formation of a long-lasting acceptance. Furthermore, acceptance may be based mainly on economic criteria, on usefulness, on ecological or even aesthetic aspects. It can be shown that not all of these motivations lead to a long-lasting acceptance. Ecologically based acceptance seems the most promising because it is founded on general convictions. Economic incentives--though important--seem to generate only superficial acceptance and do not seem to be as important as is usually assumed.