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101.
Suwat Saengkerdsub Arunachalam Muthaiyan Jody M. Lingbeck Corliss A. O’Bryan Philip G. Crandall Steven C. Ricke 《Journal of environmental science and health. Part. B》2013,48(4):290-298
Methionine is the first limiting amino acid in poultry feed. Currently, methionine supplement is synthesized from an expensive chemical process requiring hazardous chemicals. Therefore, the objectives of this study were isolation of methionine producing bacteria from environmental samples and quantification of methionine production in these isolated bacteria. MCGC medium was selected as the isolation medium for methionine-producing bacteria by using Corynebacterium glutamicum ATCC13032 and Escherichia coli ATCC23798 as the positive and negative controls, respectively. Thirty-nine bacterial strains were obtained from environmental samples. Only strains A121, A122, A151 and A181 were able to tolerate up to 0.1% (w/v) of ethionine or norleucine. These isolated strains were identified by sequencing small subunit rRNA genes. The results revealed that bacterial strains A121, A122, A151and A181 were Klebsiella species, Acinetobacter baumannii, A. baumannii and Pseudomonas aeruginosa, respectively. When methionine production in strains A121 and A181 was quantitated, strains A121 and A181 generated methionine up to 31.1 and 124.6 μg/ml, respectively. 相似文献
102.
Bryan P. Wallace Jeffrey A. Seminoff Susan S. Kilham James R. Spotila Peter H. Dutton 《Marine Biology》2006,149(4):953-960
Oceanographic sampling is often limited to local and temporally concise assessments of complex, transient, and widespread phenomena. However, long-lived, migratory pelagic vertebrates such as leatherback turtles (Dermochelys coriacea, Vandelli 1761) can provide important integrated information about broad-scale oceanographic processes. Therefore, the present study analyzed stable carbon and nitrogen isotope ratios (δ13C and δ15N) of egg yolk and red blood cells from nesting leatherback populations from Costa Rica in the eastern Pacific in 2003–2004 and 2004–2005 and from St. Croix in the North Atlantic in 2004 and 2005 to establish differences between nutrient sourcing and its influence on higher trophic level consumers in both ocean basins. Whereas δ13C signatures were similar between Costa Rica (−19.1±0.7‰) and St. Croix (−19.4±1.0‰) leatherbacks, reflecting the pelagic foraging strategy of the species, Costa Rica leatherback δ15N signatures (15.4±1.8‰) were significantly enriched relative to St. Croix leatherback δ15N signatures (9.8±1.5‰). This δ15N difference likely reflects inter-basin differences in nitrogen cycling regimes and their influence on primary productivity being transferred through several trophic levels. Thus, high-order marine consumer movements, habitat preferences, and stable isotope signatures can be combined with ocean sampling to elucidate interactions between oceanographic processes and marine megafauna.Electronic Supplementary Material Supplementary material is available for this article at and is accessible for authorized users. 相似文献
103.
Cross-correlation analysis is the most valuable and widely used statistical tool for evaluating the strength and direction
of time-lagged relationships between ecological variables. Although it is well understood that temporal autocorrelation can
inflate estimates of cross correlations and cause high rates of incorrectly concluding that lags exist among time series (i.e.
type I error), in this study we show that a problem we term intra-multiplicity can cause substantial bias in cross-correlation analysis even in the absence of autocorrelation. Intra-multiplicity refers
to the numerous time lags examined and cross-correlation coefficients computed within a pair of time series during cross-correlation
analysis. We show using Monte Carlo simulations that intra-multiplicity can spuriously inflate estimates of cross correlations
by identifying incorrect time lags. Further, unlike autocorrelation, which generally identifies lags close to the true lag,
intra-multiplicity can erroneously identify lags anywhere in the time series and commonly results in a direction change of
the correlation (i.e. positive or negative). Using Monte Carlo simulations we develop formulas that quantify the bias introduced
by intra-multiplicity as a function of sample size, true cross correlation between the series, and the number of time lags
examined. A priori these formulas enable researchers to determine the sample size needed to minimize the biases introduced
by intra-multiplicity. A posteriori the formulas can be used to predict the expected bias and type I error rate associated
with the data at hand, as well as the maximum number of time lags that can be analyzed to minimize the effects of intra-multiplicity.
We examine the relationship between commercial catch of chum salmon and surface temperatures of the North Pacific (1925–1992)
to illustrate the problems of intra-multiplicity in fisheries studies and the application of our formulas. These analyses
provide a more robust framework to assess the temporal relationships between ecological variables.
Received: 28 July 2000 / Accepted: 6 December 2000 相似文献
104.
Bryan L. Williams Hoi K. Suen Sarah E. Rzasa Tanya Heikkila Maria Pennock-Roman 《Journal of Environmental Planning and Management》2003,46(4):499-522
This study was conducted to identify factors that influence individuals' acceptance of environmental management technologies for cleaning up hazardous materials. The study sample consisted of approximately 2600 residents living within emergency response zones surrounding eight US Army's Chemical Weapons Stockpile sites. The findings suggest that residents perceive clear differences between the desirable characteristics of the two technologies: incineration and neutralization. In a relative comparison, the majority of positive technological attributes were associated with incineration. Positive perceptions toward incineration were associated with individuals who trust the Army, who perceive that the media have made them more trusting of weapons disposal activities, who are ready to participate, and who are male. Unlike incineration, there was insufficient evidence that individual factors influence variations in perceptions toward neutralization. No community factor was related to perceptions toward either incineration or neutralization. 相似文献
105.
Nervous system active pharmaceutical ingredients (APIs), including anti-depressants and opioids, are important clinically administered pharmaceuticals within healthcare facilities. This study provides source characterization data describing the composition and magnitude of nervous system APIs present in healthcare facility wastewaters. Concentrations and mass loadings of ten nervous system APIs and three nervous system API metabolites are reported for wastewaters from a hospital, nursing, assisted living, and independent living facility within a single municipality. Concentrations of nervous system APIs ranged from non-detectable levels for alprazolam in all four facility wastewaters to a high of 290 ng/L amitriptyline in nursing facility wastewater. The summed mean concentration of all thirteen analytes ranged from 402 ng/L in independent living facility wastewater to 624 ng/L in assisted living facility wastewater. Wastewater flow rates from each facility were combined with concentration data to estimate the daily mass loading of nervous system APIs leaving each facility through wastewater discharge to the municipal sewer system. The total mass loading of all thirteen analytes for the hospital, nursing, assisted living, and independent living facility was 228, 44, 29.5, and 28.1 mg/day, respectively. The total mass loading of nervous system APIs contributed to the municipality's wastewater from all four facilities was 330 mg/day. 相似文献
106.
Woodbury BL Eigenberg RA Varel V Lesch S Spiehs MJ 《Journal of environmental quality》2011,40(5):1416-1422
Subsurface measures have been adapted to identify manure accumulation on feedlot surfaces. Understanding where manure accumulates can be useful to develop management practices that mitigate air emissions from manure, such as odor or greenhouse gases. Objectives were to determine if electromagnetic induction could be used to predict differences in volatile fatty acids (VFA) and other volatiles produced in vitro from feedlot surface material following a simulated rain event. Twenty soil samples per pen were collected from eight pens with cattle fed two different diets using a predictive sampling approach. These samples were incubated at room temperature for 3 d to determine fermentation products formed. Fermentation products were categorized into acetate, straight-, branched-chained, and total VFAs. These data were used to develop calibration prediction models on the basis of properties measured by electromagnetic induction (EMI). Diet had no significant effect on mean volatile solids (VS) concentration of accumulated manure. However, manure from cattle fed a corn (Zea mays L.)-based diet had significantly ( P ≤ 0.1) greater mean straight-chained and total VFA generation than pens where wet distillers grain with solubles (WDGS) were fed. Alternately, pens with cattle fed a WDGS-based diet had significantly (P ≤ 0.05) greater branched-chained VFAs than pens with cattle fed a corn-based diet. Many branched-chain VFAs have a lower odor threshold than straight-chained VFAs; therefore, emissions from WDGS-based diet manure would probably have a lower odor threshold. We concluded that diets can affect the types and quantities of VFAs produced following a rain event. Understanding odorant accumulation patterns and the ability to predict generation can be used to develop precision management practices to mitigate odor emissions. 相似文献
107.
A well-drained soil in N-fertilized dairy pasture was amended with particulate organic carbon (POC), either sawdust or coarse woody mulch, and sampled every 4 wk for a year to test the hypothesis that the addition of POC would increase denitrification activity by increasing the number of microsites where denitrification occurred. Overall mean denitrifying enzyme activity (DEA), on a gravimetric basis, was 100% greater for the woody mulch treatment and 50% greater for the sawdust treatment compared with controls, indicating the denitrifying potential of the soil was enhanced. Despite differences in DEA, no difference in denitrification rate, as measured by the acetylene block technique, was detected among treatments, with an average annual N loss of ~22 kg N ha yr Soil water content overall was driving denitrification in this well-drained soil as regression of the natural log of volumetric soil water content (VWC) against denitrification rate was highly significant ( = 0.74, < 0.001). Addition of the amendments, however, had significant effects on the availability of both C and N. An additional 20 to 40 kg N ha was stored in POC-amended treatments as a result of increases in the microbial biomass. Basal respiration, as a measure of available C, was 400% greater than controls in the sawdust treatment and 250% greater than controls in the mulch. Net N mineralization, however, was significantly lower in the sawdust treatment, resulting in significantly lower nitrate N levels than in the control. We attribute the lack of measured response in denitrification rate to the high temporal variability in denitrification and suggest that diffusion of nitrate may ultimately have limited denitrification in the amended treatments. Our data indicate that manipulation of denitrification by addition of POC may be possible, particularly when nitrate levels are high, but quantifying differences in the rate of denitrification is difficult because of the temporal nature of the process (particularly the complex interaction of N availability and soil water content). 相似文献
108.
Bryan E Wright Susan D Riemer Robin F Brown Aicha M Ougzin Katherine A Bucklin 《Ecological applications》2007,17(2):338-351
The populations of many native species have increased or expanded in distribution in recent decades, sometimes with negative consequences to sympatric native species that are rarer or less adaptable to anthropogenic changes to the environment. An example of this phenomenon from the Pacific Northwest is predation by locally abundant pinnipeds (seals and sea lions) on threatened, endangered, or otherwise depleted salmonid (Oncorhynchus spp.) populations. We used survey sampling methodology, acoustic telemetry, and molecular genetics to quantify the amount of harbor seal (Phoca vitulina) predation on a depressed run of coho salmon (O. kisutch) and to determine whether some seals consumed a disproportionately higher number of salmonids than others. Based on a probability sample totaling 759.5 h of observation, we estimated that seals consumed 1161 adult salmonids (95% CI = 503-1818 salmonids) during daylight hours over an 18.9-km estuarine study area in Oregon during an 84-d period in fall 2002. Simultaneous tracking of 56 seals via an acoustic telemetry array indicated that a small proportion of marked seals (12.5%) exhibited behavior that was consistent with specialization on salmonids. These seals spent the majority of their time in the riverine portion of the study area and did so disproportionately more at night than day. Genetic analysis of 116 salmonid structures recovered from 11 seal fecal samples suggested that coho salmon accounted for approximately one-half of total salmonid consumption. Though subject to considerable uncertainty, the combined results lead us to infer that seals consumed 21% (range = 3-63%) of the estimated prespawning population of coho salmon. We speculate that the majority of the predation occurred upriver, at night, and was done by a relatively small proportion of the local seal population. Understanding the extent and nature of pinniped predation can provide important inputs into risk assessments and other modeling efforts designed to aid the conservation and recovery of salmonids in the Pacific Northwest. Such understanding may also help inform management actions designed to reduce the impact of pinniped predation on salmonids, which potentially range from short-term lethal removal programs to long-term ecosystem restoration and protection efforts. 相似文献
109.
110.
Relationships among Isolated Wetland Size, Hydroperiod, and Amphibian Species Richness: Implications for Wetland Regulations 总被引:6,自引:0,他引:6
Joel W. Snodgrass †‡ Mark J. Komoroski † A. Lawrence Bryan JR. † Joanna Burger 《Conservation biology》2000,14(2):414-419
Abstract: Wetland development within the United States is regulated primarily by size. Decisions concerning wetland destruction or conservation are therefore based in part on three inherent assumptions: (1) small wetlands contain water for short portions of the year; (2) small wetlands support few species; and (3) species found in small wetlands are also found in larger wetlands. We tested these assumptions using data on wetland size, relative hydroperiod (drying scores), and relative species richness of amphibians in depression wetlands of the southeastern United States. We found a significant (p = 0.03) but weak (r2 = 0.05) relationship between hydroperiod and wetland size and no relationship (p = 0.48) between amphibian species richness and wetland size. Furthermore, synthetic models of lentic communities predict that short-hydroperiod wetlands support a unique group of species. Empirical investigations support this prediction. Our results indicate that hydroperiod length should be included as a primary criterion in wetland regulations. We advocate a landscape approach to wetlands regulation, focused in part on conserving a diversity of wetlands that represent the entire hydroperiod gradient. 相似文献