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1.
Amphibian metamorphosis assays are used to evaluate potential effects of endocrine disrupting compounds on the thyroid hormone axis. In this study, Xenopus laevis tadpoles are kept in a solution of 0.2% thiourea (TU) to arrest and synchronise them in their development. The advantage of this synchronized amphibian metamorphosis assays is that synchronised tadpoles are available at any time to start metamorphosis experiments, and experimental groups are much more homogenous at the start of experimental exposure compared with groups selected from an untreated pool of animals. The water volume per animal was kept constant throughout the experimental period to overcome the influence of declining numbers of animals per aquarium due to metamorphosis and mortality on the density dependent development of the remaining tadpoles. Clophen A50 (a technical PCB mixture), the single congener 3,3',4,4'-tetrachlorobiphenyl (PCB 77) and apolar sediment extracts that were previously tested positive in the T-Screen, an in vitro proliferation assay for thyroid hormone disruption, were tested in the Synchronized Amphibian Metamorphosis Assay. Endpoints studied were mortality, malformations, body weight, and percentage of metamorphosed froglets at the end of the 60-day experimental period, percentage of tadpoles in different developmental stages, and developmental stage-dependent awarded penalty points. Dietary exposure to Clophen A50 (0.2-50mg/kg food) resulted in a significant increased percentage of tadpoles that did not pass metamorphosis at concentrations higher than 2mg/kg food. Time until metamorphosis in those animals that were able to metamorphose after the 60-days experimental period was significantly decreased. Dietary exposure to PCB 77, a congener that can be readily metabolised, did not result in significant effects in any exposure group (2-500 microg/kg food). Apolar sediment extracts from two of the three sites that are contaminated with a wide variety of chemicals significantly decreased the percentage of metamorphosed animals and significantly increased the number of tadpoles that remained in early and late metamorphic stages. These effects already occurred when the extracts where diluted more than 1000 times (on an organic carbon base) compared to environmental concentrations. The rank of potency was comparable to results obtained with the T-screen. This suggests the presence of thyroid hormone disrupting compounds in the aquatic environment and possible effects of such compounds on animal development in the wild. 相似文献
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西印度洋(WIO)地区以其迷人的海岸带、丰富的海洋生物多样性以及富饶的海洋和海岸带资源而著称.但是地处WIO地区的许多国家像肯尼亚、莫桑比克、索马里、南非、坦桑尼亚、科摩罗、马达加斯加、毛里求斯、留尼旺、塞舌尔等却极度贫困,特别是近二三十年以来,该地区的环境退化现象以及自然资源和生物多样性的下降越来越明显. 相似文献
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The Science of Nature - 相似文献
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Jonathan M. H. Green Gemma R. Cranston William J. Sutherland Hannah R. Tranter Sarah J. Bell Tim G. Benton Eva Blixt Colm Bowe Sarah Broadley Andrew Brown Chris Brown Neil Burns David Butler Hannah Collins Helen Crowley Justin DeKoszmovszky Les G. Firbank Brett Fulford Toby A. Gardner Rosemary S. Hails Sharla Halvorson Michael Jack Ben Kerrison Lenny S. C. Koh Steven C. Lang Emily J. McKenzie Pablo Monsivais Timothy O’Riordan Jeremy Osborn Stephen Oswald Emma Price Thomas David Raffaelli Belinda Reyers Jagjit S. Srai Bernardo B. N. Strassburg David Webster Ruth Welters Gail Whiteman James Wilsdon Bhaskar Vira 《Sustainability Science》2017,12(2):319-331
Delivering access to sufficient food, energy and water resources to ensure human wellbeing is a major concern for governments worldwide. However, it is crucial to account for the ‘nexus’ of interactions between these natural resources and the consequent implications for human wellbeing. The private sector has a critical role in driving positive change towards more sustainable nexus management and could reap considerable benefits from collaboration with researchers to devise solutions to some of the foremost sustainability challenges of today. Yet opportunities are missed because the private sector is rarely involved in the formulation of deliverable research priorities. We convened senior research scientists and influential business leaders to collaboratively identify the top forty questions that, if answered, would best help companies understand and manage their food-energy-water-environment nexus dependencies and impacts. Codification of the top order nexus themes highlighted research priorities around development of pragmatic yet credible tools that allow businesses to incorporate nexus interactions into their decision-making; demonstration of the business case for more sustainable nexus management; identification of the most effective levers for behaviour change; and understanding incentives or circumstances that allow individuals and businesses to take a leadership stance. Greater investment in the complex but productive relations between the private sector and research community will create deeper and more meaningful collaboration and cooperation. 相似文献
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Damon M. Hall Gerardo R. Camilo Rebecca K. Tonietto Jeff Ollerton Karin Ahrné Mike Arduser John S. Ascher Katherine C. R. Baldock Robert Fowler Gordon Frankie Dave Goulson Bengt Gunnarsson Mick E. Hanley Janet I. Jackson Gail Langellotto David Lowenstein Emily S. Minor Stacy M. Philpott Simon G. Potts Muzafar H. Sirohi Edward M. Spevak Graham N. Stone Caragh G. Threlfall 《Conservation biology》2017,31(1):24-29
Research on urban insect pollinators is changing views on the biological value and ecological importance of cities. The abundance and diversity of native bee species in urban landscapes that are absent in nearby rural lands evidence the biological value and ecological importance of cities and have implications for biodiversity conservation. Lagging behind this revised image of the city are urban conservation programs that historically have invested in education and outreach rather than programs designed to achieve high‐priority species conservation results. We synthesized research on urban bee species diversity and abundance to determine how urban conservation could be repositioned to better align with new views on the ecological importance of urban landscapes. Due to insect pollinators’ relatively small functional requirements—habitat range, life cycle, and nesting behavior—relative to larger mammals, we argue that pollinators put high‐priority and high‐impact urban conservation within reach. In a rapidly urbanizing world, transforming how environmental managers view the city can improve citizen engagement and contribute to the development of more sustainable urbanization. 相似文献
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Balgobin Nandram Emily Berg Wendy Barboza 《Environmental and Ecological Statistics》2014,21(3):507-530
Historically, the National Agricultural Statistics Service crop forecasts and estimates have been determined by a group of commodity experts called the Agricultural Statistics Board (ASB). The corn yield forecasts for the “speculative region,” ten states that account for approximately 85 % of corn production, are based on two sets of monthly surveys, a farmer interview survey and a field measurement survey. The members of the ASB subjectively determine a forecast on the basis of a discussion of the survey data and auxiliary information about weather, average planting dates, and crop maturity. The ASB uses an iterative procedure, where initial state estimates are adjusted so that the weighted sum of the final state estimates is equal to a previously-determined estimate for the speculative region. Deficiencies of the highly subjective ASB process are lack of reproducibility and a measure of uncertainty. This paper describes the use of Bayesian methods to model the ASB process in a way that leads to objective forecasts and estimates of the corn yield. First, we use small area estimation techniques to obtain state-level forecasts. Second, we describe a way to adjust the state forecasts so that the weighted sum of the state forecasts is equal to a previously-determined regional forecast. We use several diagnostic techniques to assess the goodness of fit of various models and their competitors. We use Markov chain Monte Carlo methods to fit the models to both historic and current data from the two monthly surveys. Our results show that our methodology can provide reasonable and objective forecasts of corn yields for states in the speculative region. 相似文献