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红霉素对菲降解菌GY2B的毒性及抗生素后效应 总被引:2,自引:0,他引:2
为了解抗生素对环境污染物高效降解菌的生态效应,通过红霉素对菲高效降解菌GY2B的毒性实验以及抗生素后效应(PAE)的测定,研究了菌体细胞在红霉素作用下的生理、毒理指标以及后效应期间菌体的变化。结果表明,GY2B对红霉素的最高耐受(99.9%死亡率)浓度为25μg/mL,长期接触低于25μg/mL的红霉素可降低GY2B对药物敏感性,使其产生耐药性;当红霉素浓度高于最低抑菌浓度(0.25μg/mL)时,能显著降低菌体细胞的总超氧化物歧化酶(SOD)活力,引起活性氧对菌体的损伤,亦能显著降低乳酸脱氢酶(LDH)活力,但对菌体细胞有氧呼吸水平的影响会随着时间逐渐减弱;GY2B在短暂接触红霉素后2~3 h即可复苏生长(菌密度增加10倍),但红霉素能对GY2B产生明显的后效应,诱发细胞SOS修复机制和自溶现象,使得DNA含量发生显著变化。因此,环境中抗生素对污染物降解微生物的生态效应值得关注。 相似文献
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Gavina JM Rubab M Zhang H Zhu J Nong A Feng YL 《Journal of environmental monitoring : JEM》2011,13(11):3145-3155
DNA damage represents a potential biomarker for determining the exposure risk to chemicals and may provide early warning data for identifying chemical hazards to human health. Here, we have demonstrated a simple chromatography-based method that can be used to rapidly screen for the presence of chemical hazards as well as to determine parameters relevant to hazard assessment. In this proof-of-principle study, a simple in vitro system was used to determine the interaction of pollutants and probable carcinogens, phenyl glycidyl ether (PGE), tetrachlorohydroquinone (Cl(4)HQ), methylmethane sulfonate (MMS), styrene-7,8-oxide (SO), and benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE), a metabolite of benzo[a]pyrene (B[a]P), with single- and double-stranded DNA probes. Differences in potency and reaction kinetics were studied for chemical and DNA type. A relative interaction potency equivalency (PEQ) of a chemical was determined by ratio of interaction potency of a chemical to BPDE as the reference chemical in the reaction with single- and double-stranded oligodeoxynucleotides. PEQs were found to be BPDE > PGE > SO > MMS > Cl(4)HQ for single-stranded oligodeoxynucleotides while they were found to be BPDE > PGE > Cl(4)HQ > MMS > SO for double-stranded oligodeoxynucleotides. Kinetics evaluation revealed that BPDE reacted with both DNA probes at a significantly faster rate, as compared to the remaining test chemicals. Equilibrium was reached within an hour for BPDE, but required a minimum of 48 h for the remaining chemicals. First-order rate constants were (1.61 ± 0.2) × 10(-3) s(-1) and (3.18 ± 0.4) × 10(-4) s(-1) for reaction of BPDE with double- and single-stranded DNA, respectively. The remaining chemicals possessed rate constants from 2 to 13 × 10(-6) s(-1) with a relative kinetic order for reaction with DNA of BPDE ? MMS > SO > PGE > Cl(4)HQ for ds-DNA and BPDE ? SO ≈ Cl(4)HQ ≈ MMS > PGE for ss-DNA. We further found that the reaction potency, defined by dose-response between chemical pollutants and DNA, depends on the form of DNA present for reaction. Noteworthy, we found that relative PEQ did not follow the same kinetic trends. However, our preliminary findings suggest that reaction kinetics, in combination with relative interaction potency, may be a significant parameter that can be used to evaluate the hazard level of environmental pollutants. 相似文献
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粮食生产潜力中、长期预测的目的是为国家中、长期粮食生产规划提供科学依据。粮食生产潜力中、长期预测的“双向预测理论”:从若干个预测模型中选择出2个模型,一个模型预测的未来产量是持续增加的,体现产量持续增加的科技进步力量;另一个模型预测的未来产量是先增加后减少或持续减少的,体现影响产量持续增加的负面综合因素力量。应用结果表明:模型可预测未来1~10年的粮食生产潜力,平均预测误差在5%以内。大量案例证明粮食生产潜力中、长期预测的“双向预测理论”是科学的、方法是通用的、结果是实用的。 相似文献
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用模糊数学综合评判法时石灰石/石膏湿法烟气脱硫装置进行安全验收评价,并得出通过安全验收评价的综合评价结果。 相似文献
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燃煤电厂烟气排放连续监测系统验收测试问题探讨 总被引:1,自引:0,他引:1
阐述了燃煤电厂烟气排放连续监测系统在验收测试中应该注意和可能出现的问题,并提出了问题的解决办法. 相似文献
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