Linking NE1545 gene expression with cell volume changes in Nitrosomonas europaea cells exposed to aromatic hydrocarbons

Nitrosomonas europaea, a model ammonia oxidizing bacterium, was exposed to a wide variety of aromatic hydrocarbons in 3 h batch assays. The expression of NE1545, a phenol sentinel gene involved in fatty acid metabolism, was monitored via quantitative real-time polymerase chain reaction (qRT-PCR) and a Coulter Counter technique was used to monitor changes in cell volume. Decreases in cell volume and NE1545 gene expression correlated strongly with exposure to aromatic hydrocarbons that possessed a single polar group substitution (e.g. phenol and aniline). Aromatic hydrocarbons that contain no polar group substitutions (e.g. toluene) or multiple polar group substitutions (e.g. p-hydroquinone) caused negligible changes in NE1545 expression and cell volume. The oxidation of aromatic hydrocarbons by N. europaea from configurations without a single polar group to one with two polar groups (e.g. p-cresol oxidized to 4-hydroxybenzyl alcohol) and from configurations with no polar groups to one with a single polar group (e.g. ethylbenzene oxidized to 4-ethylphenol) greatly influenced NE1545 gene expression and observed changes in cell volume. Nitrification inhibition in N. europaea by the aromatic hydrocarbons was found to be completely reversible; however, the decreases in cell volume were not reversible suggesting a physical change in cell membrane composition. Ammonia monooxygenase blocking studies showed that the chemical exposure that was responsible for the cell volume decrease and up-regulation in gene expression and not the observed inhibition. N. europaea is the first bacterium shown to experience significant changes in cell volume when exposed to μM concentrations of aromatic hydrocarbons, three orders of magnitude lower than previous studies with other bacteria.     

State Standards,Regulations, and Responsibilities in Noise Pollution Control

In the near future the State of Minnesota will adopt noise pollution standards and regulations based on the findings presented in this paper. Comprehensive noise standards are presented for all sources of noise, including airports, highways, and industrial sources. Two of the three dimensions of noise—intensity and frequency—are measured by the use of the unit dBA, whereas the third dimension—duration—is measured by use of the temporal distribution, expressed as L_x, where x is the sound pressure level in dBA exceeded x% of the time. Standards are established for five zones: 1. residential—single family, 2. residential— apartments, public buildings, 3. commercial, 4. manufacturing, and 5 industrial. The L_x standards apply to most noises including industrial and highway noises. Aircraft noise and impulse noise standards are also discussed. The six phase implementation plan for noise abatement is discussed.     

Gonadal morphology and gametogenesis in the sea pen Pennatula aculeata (Anthozoa: Pennatulacea) from the Gulf of Maine

The ultrastructural features of gametogenesis are described in male and female colonies of the sea pen Pennatula aculeata. Specimens were collected for observation and fixation at 113 to 231 m depth in the Gulf of Maine, USA, in August 1993. The species is gonochoric, and all stages of gametogenesis are observed in both male and female colonies >45 mm in height. Gametogenesis shows several features that differ from sea anemones. The developing oocytes and sperm cysts are completely encompassed by gastrodermally derived follicle cells, and they are released from the mesenteries into the coelenteron before they are fully differentiated. Following maturation in the coelenteron, the eggs and intact sperm cysts are expelled through the mouths of the autozoids during spawning. The expulsion of sperm cysts suggests that they function as primitive spermatophores, perhaps as a way of reducing sperm dilution. Vitellogenesis results in the biosynthesis of lipid droplets which are the sole nutrient reserves in the egg. Heterosynthetic vitellogenesis is characterized by the importation of lipid precursors into the oocyte, and there is some indirect evidence that hypertrophic follicle cells play a role in production, transport, and/or mediation of these precursors. Spermatogenesis is similar to that of other anthozoans. The spermatozoon has a cone-shaped head, a posterior nuclear fossa, a ring of lipid-like bodies in the midpiece, a prominent cytoplasmic collar surrounding the proximal flagellum, and a single mitochondrion, but the posterior region of the sperm also contains previously undescribed concentric rings of cisternae resembling smooth endoplasmic reticulum. Received: 23 March 1998 / Accepted: 31 July 1998     

Visiting a Climate-Influenced National Park: The Stability of Climate Change Perceptions

Understanding perceptions of global environmental issues, such as climate change, can help inform resource management, policy development, and communication with constituents. Although a considerable amount of research documents citizens’ perceptions of climate change, few have investigated how interactions with climate-impacted parks and protected areas influence these perceptions, and consequently elements of environmental management. Using a mixed methods Instrument Development Approach, the researchers examined the stability of park visitors’ (N = 429) climate change perceptions during a daylong interaction with climate-sensitive and influenced resources at Kenai Fjords National Park in Alaska. Results indicate that global-level beliefs about climate change remained relatively stable during a park experience, but perceptions about climate change at the park-level (e.g., impacts) appeared more malleable. Findings also revealed the type of park experience (terrestrial vs. marine) can influence the degree of change in visitors’ perceptions. Implications for communication, outreach, and park management are discussed.     

The Arkansas Valley “Super Ditch”— An Analysis of Potential Economic Impacts1

McMahon, Tyler G. and Mark Griffin Smith, 2012. The Arkansas Valley “Super Ditch”— An Analysis of Potential Economic Impacts. Journal of the American Water Resources Association (JAWRA) 00(0):000‐000. 1‐12. DOI: 10.1111/jawr.12005 Abstract: In Colorado’s Arkansas River basin, urban growth and harsh farming conditions have resulted in water transfers from agricultural to urban uses. Several studies have shown that these transfers have significant secondary economic impacts associated with the removal of irrigated land from production. In response, new methods of sharing water are being developed to allow water transfers that benefit both farm and urban economies, compared with previous permanent transfers that negatively impacted surrounding farm communities. One such project currently under development is the Arkansas Valley “Super Ditch,” which is a rotational crop fallowing plan based on long‐term water leasing designed to provide an annual supply of 25,000 acre‐feet of water (31.6 Mm³). This article analyzes the net benefits of implementing the “Super Ditch” for both the farmers and the surrounding community.     

Reproduction, dispersal and settlement of the bathyal ophiuroid Ophiocten gracilis in the NE Atlantic Ocean

  Ophiocten gracilis is an ophiuroid found at bathyal depths in the North Atlantic Ocean. The adults show strong seasonal reproduction, with an ophiopluteus in the surface plankton. Settling postlarvae were collected in sediment traps moored at 1000 and 1400 m depth in the NE Atlantic during Julian Days 142 to 212 (May to July) in 1996. During this period, growth of postlarvae in the traps was linear and the diet consisted of phytodetritus and foraminifera. Experiments suggest that postlarvae sink at rates of up to 500 m d⁻¹, although this may well be slower in the natural environment. The high fecundity, seasonality and high population density resulted in high fertilization success, and many of the offspring were advected outside the normal adult range, where they were able to settle but did not survive to adulthood. Received: 8 October 1999 / Accepted: 8 May 2000     

Solid-Phase Microextraction As A Tool for Studying Volatile Compounds in Frog Skin

Solid-phase microextraction (SPME) is an effective technique for studying frog volatile secretions. Its primary advantage is in its application to sampling live animals. the ability to sample an organism over an extended period allows changes in an individual's chemical signature to be determined. the presence of eucalyptol in the skin secretion of Ewing's tree frog, Litoria ewingi, was used to assess the effectiveness of SPME in sampling frog volatiles. Rapid sample times coupled with the polydimethylsiloxane/divinylbenzene (PDMS/DVB) fibre provided the best signal/noise ratio for the majority of frog volatiles analysed, and importantly resulted in the least amount of stress to the animals involved.     

Process‐orientation versus outcome‐orientation during organizational change: the role of organizational identification

In this paper we argue that organizational identification is predictive of employee interests and concerns during periods of organizational change. More specifically, we assert that organizational identification may largely determine whether employees may be focused upon the change related outcomes (e.g., salary, expenses, etc.), or on the change processes (e.g., procedures, voice and participation options, etc.). Data of both a scenario experiment and a survey are presented indicating that high and low identifiers indeed are differentially interested in process and outcome information. The results suggest that people who identify less with the organization are more likely to be focused upon the change outcomes then on the change process, while people who identify highly (i.e., deep structure) with the organization are more likely to be focused upon the change processes then on the change outcomes. The benefits of awareness of organizational members' level of identification for organizational change management are discussed. Copyright © 2006 John Wiley & Sons, Ltd.     

Growth, fire history, and browsing recorded in wood rings of shrubs in a mild temperate climate

Separate effects of abiotic and biotic factors on the structure and dynamics of ecological communities may be recorded in growth rings of woody plants. We used Ceanothus cuneatus rigidus and Arctostaphylos pumila to tease apart the roles of fire, rain, and herbivores on the histories and community structure of four areas in a coastal mediterranean-type climate in central California with mild winters and mild summers. Ring widths of both species were related to rainfall in two of the areas; heavy deer browsing on Ceanothus overwhelmed the climate signal in the others. Ceanothus germination was more closely related to heavy rainfall, especially during ENSO years, than to fire events. In a related greenhouse experiment that evaluated these observations, the same proportions of new Ceanothus seeds germinated after burning and after receiving regular water for several months, but germination of old seeds responded primarily to the fire treatment. In areas where heavy browsing by mammals reduces recruitment and growth of Ceanothus and increases mortality, the continuance of the Ceanothus population must rely heavily on germination from the persistent seed bank during unusually wet years or after occasional fires. Because Arctostaphylos can produce new stems from underground roots, individual plants may survive and produce seeds until another fire.