Chlorinated dibenzo-<Emphasis Type="Italic">p</Emphasis>-dioxins and dibenzofurans in the baltic herrinj and sprat of estonian coastal waters

BACKGROUND, AIMS AND SCOPE: The concentration of chlorinated dibenzo-p-dioxins and dibenzofurans in many fish from the Baltic requires monitoring, since it approaches or exceeds the European Union threshold limit value of 4 pg TEQ/g wet weight of fish for human consumption. The concentrations, expressed in TEQs, are important for toxicology and regulations, but hide the concentrations of the individual congeners, which are important for other environmental sciences, source allocation, and for the detection of measurement errors. This report evaluates the results of a survey reported earlier only in the terms of the TEQ concentrations. METHODS: Principal Component Analysis (PCA) was used to reduce the dimensions of the data (17 = 7 chlorinated dibenzo-p-dioxin and 10 chlorinated dibenzofuran congeners) to three principal components. This facilitated the interpretation of the congener profiles. Slopes of the congener concentrations as a function of age of the fish were estimated by least squares regression. The results were compared with a large set of data for lake trout from Lake Ontario. RESULTS AND DISCUSSION: The congener profiles of Baltic herring are less scattered than those of sprat. The profiles of herring from the central Baltic differ from those of herring from the Gulf of Riga and both appear to be affected relatively minimally by the age of the fish. The congener profiles of herring from the western Gulf of Finland are similar to those from the central Baltic, those from middle Gulf of Finland are similar to those from the Gulf of Riga. Both seem to be more affected by age of the fish than the profiles of the first two groups. The concentrations of several pentachloro- and hexachloro-dibenzo-p-dioxins and dibenzofurans increase with the age of the fish CONCLUSION: PCA is a good technique for the evaluation of the congener profiles. The resulting loading and score plots provide a good graphic summary of the multidimensional data. Additional analyses are needed to confirm the observed profile patterns. A comparison with the results of a long-term monitoring from another area shows that the age of the fish is a more important factor than the year of capture. RECOMMENDATION AND OUTLOOK: The surveys should continue for a number of years and the results should be presented and evaluated both in terms of the TEQs as well as in terms of weight concentrations. Since the concentrations do not appear to change very much from year to year, it would be better to carry out surveys only every 3-4 years and, instead, stratify the sampling according to age and gender of the fish, and to analyze replicate extracts by replicate measurements. The inclusion of unmarked replicate samples would be a good quality assurance measure. It would be desirable to analyze additional parts of the food chain in order to understand the fate of the compounds in the ecosystem.     

Antibiotic resistance and virulence factors of Escherichia coli from eagles and goshawks

One of the major global problems in medicine is microbial resistance to antibiotics (antimicrobial resistance) and this has become an increasingly frequent research topic. This study focuses on antimicrobial resistance, phylogenetic and genetic characterization of Escherichia coli from wild birds: ten isolates from eagles (Aquila chrysaetos), nine from goshawks (Accipiter gentilis) and 24 from broilers in the Slovak Republic. Twenty-two strains with presence of int1 gene were selected and examined for the presence or absence of transposon gene (tn3), genes of antibiotic resistance and virulence factors. We detected sequence type (ST) in eagles ST 442 with genes iss, papC, iutA, cvaC, tsh, fyuA, iroN, kps, feoB, sitA, irp2, ireA for virulence factors and tetA, sul1, sul2, dfrA, aadA for antibiotic resistance; in goshawks ST 1011 with iss, papC, fyuA, iroN, feoB, sitA and qnrS1, tetA, sul1, sul2, dfrA, aadA, respectively. These ST types have been found in humans too and should be evaluated further for possible zoonotic potential and transfer of resistance genes from the environment.     

Persistent organic pollutants,heavy metals and parasites in the glaucous gull (Larus hyperboreus) on Spitsbergen

The prediction of a higher parasite infection as a consequence of an impaired immune system with increasing persistent organic pollution (POP) and heavy metal levels were investigated in adult glaucous gull (Larus hyperboreus) from Svalbard. The levels of chlorinated pesticides, polychlorinated biphenyls (PCBs), toxaphenes and polybrominated diphenyl ethers (PBDEs) were measured in liver. Cupper, cadmium, lead, mercury, selenium and zinc were measured in kidney samples. An elevated ratio of PCB-118 was found, suggesting that local contamination from the settlement was detectable in the glaucous gull. Eight cestodes, four nematodes, two acanthocephalan and three trematode helminth species were found in the intestine. A positive correlation was found between cestode intensities and selenium levels and between acanthocephalan intensities and mercury levels. No correlation was found between parasite intensities and POP concentrations. It is concluded that the contaminant levels found in glaucous gulls do not cause immune suppression severe enough to affect parasite intensity.     

Geno-toxicity assay of sediment and water samples from the Upper Silesia post-mining areas, Poland by means of Allium-test

Genotoxic potential of two environmental compartments (water and sediment) from the Upper Silesia Coal Basin (USCB), Poland were evaluated and compared by employing root meristem cells of Allium cepa. The clear genotoxic effect of water and sediment sampled was shown, with an important contribution of severe types of cytogenetic abnormalities. The most biologically relevant pollutants were revealed through multivariate statistical analysis of relationships between biological effects registered and the environment contamination. Overall, results of simultaneous use of conventional monitoring methods and biological tests suggested that contemporary levels of persistent pollutants in post-mining areas of the USCB may enhance the risk both for human health and biological components of natural ecosystems.     

Comparative assessment of pollution by the use of industrial agricultural fertilizers in four rapidly developing Asian countries

This article describes a study of the environmental impacts of the use of industrial agricultural chemicals in four Asian countries—China, India, the Philippines and Thailand. The objective was to contribute objectively to the discussion on the extent of the problem, past and current damages to the environment to and outline possible paths to sustainable and environmentally benign agriculture. The four countries are experiencing rapid economic growth under a tremendous population growth pressure that, with the exception of China, will continue without leveling of in a foreseeable future. This requires more food production that has been accomplished by the increased use of industrial chemical fertilizers. Although the four countries uses of industrial chemicals vary, the mix of nutrients appears to be imbalanced, resulting in large nitrogen losses into the environment, especially in China. A suggested solution of the problem begins with reducing (China) or maintaining (India, the Philippines, Thailand) average nutrient application levels needed by the crops and includes optimal hybrid agriculture by using organic fertilizers, and fertilizers in the irrigation water already overloaded with nitrogen. There is a need to balance fertilizer N and P applications with the crop needs.     

Investigations on the sorption of a toxaphene model congener, the B7-1450, on marine sediments

Sorption is a natural process that takes place in sediments or soils and changes the mobility and availability of hydrophobic organic compounds, such as toxaphene pesticide in the environment. The sorption of the 2-exo,3-endo,5-exo,8,9,10,10-heptachlorobornane (B7-1450), used as a model compound of the toxaphene heptachlorobornane congeners found in sediments, was investigated for the first time through a series of batch sorption experiments. The losses of B7-1450 due to adsorption onto glass walls and to evaporation occurring during analytical treatment steps were corrected. The study showed that these specific losses ranged from 2% to 3.5% for the glass walls adsorption and can be as high as 15% for the evaporation treatment. The sorption coefficients, K(d) and K(oc), of B7-1450 could be overestimated by >30%, particularly for low-concentration samples, if the losses were not corrected. Loss correction equations were established, validated and applied to determine sorption coefficients for the B7-1450 congener. The K(oc) values for B7-1450 determined over a gradient of concentrations ranged from 3.5x10(4) to 6.5x10(4)mlg(-1), revealing a strong affinity of B7-1450 for marine sediments.     

A computationally feasible quantum chemical model for 13C NMR chemical shifts of PCB-derived carboxylic acids

Two quantum chemical models have been derived for the prediction of 13C NMR chemical shifts of novel PCB acids obtained from PCBs by catalytic carbonylation. 13C isotropic shielding constants were calculated employing the GIAO (gauge-independent atomic orbital) method with density functional theory (DFT). The best results were obtained by cluster calculations, which took the solvent effects into account properly. In this approach, a solvent molecule (acetone) was attached by a hydrogen bond to every hydrogen atom present in a PCB acid, and the geometry of the molecular cluster was optimized employing the AM1 method. For 158 chemical shifts, the cross-validated standard error was 2.8 ppm and the cross-validated correlation coefficient was 0.94.