(<Emphasis Type="Italic">Z</Emphasis>)-9-tricosene identified in rectal gland extracts of <Emphasis Type="Italic">Bactrocera oleae</Emphasis> males: first evidence of a male-produced female attractant in olive fruit fly

It is well-known that Bactrocera oleae (olive fruit fly) females attract conspecific males by using 1,7-dioxaspiro[5,5]undecane (1) as the main component of their sex pheromone, and that 1 is produced in the female rectal gland. Although some authors have claimed that B. oleae males also attract females, to date no male-produced female attractants have been found in this species. In this paper, we report the first identification of a substance unique to males and able to attract females. The findings of the study include the following: (1) females responded in a bioassay to hexane extracts obtained from rectal glands of 15-day-old B. oleae males, (2) the presence of (Z)-9-tricosene (2) was consistently and unambiguously identified in these extracts using gas chromatography (GC) and GC-mass spectrometry methods, (3) in preliminary bioactivity tests, low doses (equivalent to a few males) of chemically and stereoisomerically pure synthetic (Z)-9-tricosene (2) attracted olive fruit fly females. Interestingly, compound 2, commonly called muscalure, is also a well-known component of the house fly (Musca domestica) sex pheromone.     

A Simulation Study on the Efficacy of Advanced Belt Restraints to Mitigate the Effects of Obesity for Rear-Seat Occupant Protection in Frontal Crashes

Objective: Recent field data analyses have shown that the safety advantages of rear seats relative to the front seats have decreased in newer vehicles. Separately, the risks of certain injuries have been found to be higher for obese occupants. The objective of this study is to investigate the effects of advanced belt features on the protection of rear-seat occupants with a range of body mass index (BMI) in frontal crashes.

Methods: Whole-body finite element human models with 4 BMI levels (25, 30, 35, and 40 kg/m²) developed previously were used in this study. A total of 52 frontal crash simulations were conducted, including 4 simulations with a standard rear-seat, 3-point belt and 48 simulations with advanced belt features. The parameters varied in the simulations included BMI, load limit, anchor pretensioner, and lap belt routing relative to the pelvis. The injury measurements analyzed in this study included head and hip excursions, normalized chest deflection, and torso angle (defined as the angle between the hip–shoulder line and the vertical direction). Analyses of covariance were used to test the significance (P <.05) of the results.

Results: Higher BMI was associated with greater head and hip excursions and larger normalized chest deflection. Higher belt routing increased the hip excursion and torso angle, which indicates a higher submarining risk, whereas the anchor pretensioner reduced hip excursion and torso angle. Lower load limits decreased the normalized chest deflection but increased the head excursion. Normalized chest deflection had a positive correlation with maximum torso angle. Occupants with higher BMI have to use higher load limits to reach head excursions similar to those in lower BMI occupants.

Discussion and Conclusion: The simulation results suggest that optimizing load limiter and adding pretensioner(s) can reduce injury risks associated with obesity, but conflicting effects on head and chest injuries were observed. This study demonstrated the feasibility and importance of using human models to investigate protection for occupants with various BMI levels. A seat belt system capable of adapting to occupant size and body shape will improve protection for obese occupants in rear seats.     

Toxic effects of four sulphonylureas herbicides on soil microbial biomass

The effect of four triazinyl-sulfonylurea herbicides (cinosulfuron, prosulfuron, thifensulfuron methyl, triasulfuron) on soil microbial biomass, soil respiration, metabolic activity, metabolic quotient, and some enzymatic activities (acid and alkaline phosphatase, β-glucosidase, arylsulphatase, and fluorescein diacetate hydrolysis) were monitored under controlled conditions over 30 days. The herbicides were applied at the normal field dose (FD) and at ten-fold (10 FD) the field dose, in order to mimic a long term toxic effect. The measured soil microbial parameters showed that the FD had slight effects on soil microflora, while at 10 FD the tested herbicides exerted a stronger detrimental effect on soil microbial biomass and its biochemical activities.     

Comparative management of offshore posidonia residues: composting vs. energy recovery

Residues of the marine plant posidonia (Posidonia oceanica, PO) beached in tourist zones represent a great environmental, economical, social and hygienic problem in the Mediterranean Basin, in general, and in the Apulia Region in particular, because of the great disturb to the bathers and population, and the high costs that the administrations have to bear for their removal and disposal.In the present paper, Authors determined the heating values of leaves and fibres of PO, the main offshore residues found on beaches, and, meantime, composted those residues with mowing and olive pruning wood. The final composts were characterized for pH, electrical conductivity, elemental composition, dynamic respiration index, phytotoxicity, fluorescence and infrared spectroscopic fingerprints.The aim of the paper was to investigate the composting and energy recovery of PO leaves and fibres in order to suggest alternative solutions to the landfill when offshore residues have to be removed from recreational beaches.The fibrous portion of PO residues showed heating values close to those of other biofuels, thus suggesting a possible utilization as source of energy.At the same time, compost obtained from both PO wastes showed high quality features on condition that the electrical conductivity and Na content are lowered by a correct management of wetting during the composting.     

A fuzzy analytic network process for multi-criteria evaluation of contaminated site remedial countermeasures

The Analytic Network Process (ANP) has been proposed to incorporate interdependence and feedback effect in the prioritization of remedial countermeasures using a hierarchical network decision model, but this approach seems to be incapable of capturing the vagueness and fuzziness during value judgment elicitation. The aim of this paper is to present an evaluation method using a fuzzy ANP (FANP) approach to address this shortcoming. Triangular fuzzy numbers (TFN) and their degree of fuzziness are used in the semantic scale as human judgment expressed in natural language is most often vague and fuzzy. The method employs the alpha-cuts, interval arithmetic and optimism index to transform the fuzzy comparative judgment matrix into set of crisp matrices, and then calculates the desired priorities using the eigenvector method. A numerical example, which was drawn from a real-life case study of an uncontrolled landfill in Japan, is presented to demonstrate the process. Results from the sensitivity analysis describe how the fuzziness in judgment could affect the solution robustness of the prioritization method. The proposed FANP approach therefore could effectively deal with the uncertain judgment inherent in the decision making process and derive the meaningful priorities explicitly from a complex decision structure in the evaluation of contaminated site remedial countermeasures.     

Air pollutants and the characterization of the organic content of aerosol particles in a mixed industrial/semi-rural area in central Italy

Both regulated and unregulated air pollutants were detected during an intensive seasonal sampling campaign in a mixed industrial/semi-rural area on the outskirts of Rome, Italy, at two sites located opposite a hospital waste incinerator, downwind according to the direction of the prevailing local winds. Concentrations of pollutants were significantly lower than in urban atmospheres. The composition of particulate organic material indicated a heavy biogenic impact, accompanied by a lower contribution from petroleum-related processes. Both PAH and nitro-PAH group compositions of particulates were used to assess the nature and relative importance of sources. Both sites showed that different and diffuse sources contributed to local pollution with a significant contribution from traffic, proving that the hospital waste incinerator was not the main pollution source in this area. Among unregulated compounds, a series of positional isomers of nitro-PAHs and other organic compounds associated with particulate matter were investigated. In particular, 1- and 3-nitrophenanthrene identification was carried out, and they proved to be the most abundant nitro-PAHs.     

Chemical characteristics of organic aerosol in Bab-Ezzouar (Algiers). Contribution of bituminous product manufacture

The organic compositions of atmospheric particulate matter from Bab-Ezzouar (Algiers) have been investigated to assess the air pollution levels suspected to be caused by asphalt product and yeast manufactures. After a medium-volume air sampling, soxhlet extraction, alumina elution and HPLC separation, the extracts were analysed by high-resolution gas chromatography (HRGC) and gas chromatography coupled to mass spectrometry (GC-MS). The composition of n-alkane and polycyclic aromatic hydrocarbons (PAH) fractions reflected the petrogenic origin from the emission of asphalt materials production in addition to vascular plant wax emissions. In contrast, microbial activities seemed to play the main role for the presence of n-alkanoic acids at Bab-Ezzouar. The sole nitrated polycyclic aromatic hydrocarbons (NPAH) observed, i.e., 2-nitrofluoranthene (2NFA), was very likely to arise from gas-phase photochemical reaction of parent PAH in the atmosphere. The total aerial levels ranged from 75 to 206 ng m(-3) for n-alkanes, from 153 to 345 ng m(-3) for n-alkanoic acids and from 44 to 100 ng m(-3) for PAH and NPAH. Although the samples were collected during the hot season, the levels of these pollutants seemed to be important and of environmental concern, especially for PAH species.     

Multiple perspectives of congruence: relationships between value congruence and employee attitudes

The relative importance of multiple types of value congruence—person–environment (P‐E), person–person (P‐P), and perceptual fit—across two hierarchical levels (workgroup and manager) and across multiple dimensions of values was investigated in a sample of 951 employees from 113 bank branches. As expected, different types of value congruence and different value dimensions were differentially important for outcomes. Both P‐E fit (between an individual's personal values and the cultural values of the organization) and perceptual fit (between an individual's perception of the organization's values and the organization's values as perceived by others) were found to be related to satisfaction, commitment, and turnover intentions, while P‐P fit (between an individual's personal values and the personal values of others) was not. Further, P‐E congruence results were generally stronger for fit with the workgroup than fit with the manager and results were stronger for the rational goal dimension which focused on external customer service. In contrast, results for perceptual fit revealed that fit was generally more important for fit with the manager than fit with the workgroup and was generally more important for the open system dimension which focused on flexibility and innovation. Copyright © 2005 John Wiley & Sons, Ltd.     

Carrier detection of duchenne muscular dystrophy through analysis of dna from deciduous teeth of a dead affected child

The sister of a child affected by Duchenne muscular dystrophy (DMD) was referred for genetic counselling to assess the risk of her being a carrier. Her brother had died 15 years previously at the age of 8. There were no other affected males in the family. There were no methods for DNA investigation at the time of the child's death and the family had never been studied for linkage with polymorphic probes on the chromosomal region Xp21. The only tissue from which an assessment of the risk could be made by DNA linkage analysis was two of the child's deciduous teeth that the parents had kept. DNA was extracted using a protocol described for the recovery of ancient DNA from museum specimens and archaeological finds. Multiplex amplification did not reveal deletions in 19 exons spanning the hot-spot regions for deletions within the dystrophin gene in Xp21. Linkage analysis using three highly polymorphic microsatellites demonstrated that the sister had not received the X chromosome borne by her brother. These results show that DNA extracted from teeth is a reliable source for molecular diagnosis.     

hCG,AFP, and uE3 patterns in the 14–20th weeks of Down's syndrome pregnancies

A marked gestational-age-dependent variation in the 15-20th week pattern of human chorionic gonadotropin concentrations in Down's syndrome (DS) pregnancies is described and its weight in DS screening is discussed.