Erythroid-specific antibodies enhance detection of fetal nucleated erythrocytes in maternal blood

Fetal nucleated erythrocytes (NRBC) in maternal blood are a non-invasive source of fetal DNA for prenatal genetic screening. We compared the effectiveness of three monoclonal antibodies for the separation of fetal cells from maternal blood by flow sorting. Mononuclear blood cells from 49 healthy pregnant women were incubated with antibody to CD 71, CD 36, and/or glycophorin A (GPA), employed singly or in combination with each other. These monoclonal antibodies recognize surface antigens on haematopoietic precursor cells. Successful isolation of fetal cells was defined as detection of Y chromosomal sequences in maternal blood from women carrying male fetuses, with absence of Y sequences when female fetuses were carried. Thus, gender prediction accuracy was used as a measure of fetal cell separation. Using anti-CD 71 to isolate fetal cells, gender prediction was 57 per cent correct; with anti-CD 36, it was 88 per cent correct. Anti-GPA, an erythrocyte-specific antigen, used alone or in combination with anti-CD 71 or 36, improved gender prediction to 100 per cent. We conclude that antibody to GPA improves the retrieval of fetal NRBC from maternal blood, permitting genetic analysis by the polymerase chain reaction.     

一个特大城市:墨西哥城大都市区的水资源管理

引言 墨西哥城大都市区(MCMA)位于被称作"墨西哥谷(Valle deMexico)"的天然围合的盆地里,海拔2240m.它下辖联邦区和墨西哥州的34个自治市.墨西哥城是墨西哥的首都,位于联邦区(DistritoFederal,DF),四面环山,海拔高度超过5000m.墨西哥州是墨西哥人口最稠密的地区(据报道有1300万居民),人口仅次于它的是墨西哥城,大约有860万人[1].     

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Mitigation and Adaptation Strategies for Global Change -