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101.
Jae-Sung Rhee Bo-Mi Kim Chang-Bum Jeong Kenneth Mei Yee Leung Gyung Soo Park Jae-Seong Lee 《Chemosphere》2013
To utilize the GST-S protein as a useful biomarker for environmental contamination, we developed a polyclonal antibody-based enzyme-linked immunosorbent assay (ELISA) in the intertidal copepod Tigriopus japonicus. Two polyclonal antibodies, TJ-GST-S1 and TJ-GST-S2, were raised against two TJ-GST-S synthetic peptides. Also a recombinant TJ-GST-S protein was purified as a standard for ELISA development. Each polyclonal antibody was tested by Western blot analysis and indirect ELISA. Of two polyclonal antibodies, TJ-GST-S2 ELISA was further employed due to its wide range of detection and the limit of specificity compared to those of TJ-GST-S1 ELISA system. After exposure to 4 metals (Ag, As, Cd, and Cu) to T. japonicus, the amount of TJ-GST-S protein was significantly elevated in a concentration-dependent manner. Also, TJ-GST-S protein was upregulated at relative high concentrations of B[α]P, PCB, and TBT. In this paper, we suggest that T. japonicas ELISA for TJ-GST-S2 is useful as a potential indicator system for marine contaminants. 相似文献
102.
The oxidation of SO2 is commonly regarded as a major driver for new particle formation (NPF) in the atmosphere. In this study, we explored the connection between measured mixing ratio of SO2 and observed long-term (duration > 3 hr) and short-term (duration <1.5 hr) NPF events at a semi-urban site in Toronto. Apparent NPF rates (J30) showed a moderate correlation with the concentration of sulfuric acid ([H2SO4]) calculated from the measured mixing ratio of SO2 in long-term NPF events and some short-term NPF events (Category I) (R2 = 0.66). The exponent in the fitting line of J30 ~ [H2SO4]n in these events was 1.6. It was also found that SO2 mixing ratios varied a lot during long-term NPF events, leading to a significant variation of new particle counts. In the SO2-unexplained short-term NPF events (Category II), analysis showed that new particles were formed aloft and then mixed down to the ground level. Further calculation results showed that sulfuric acid oxidized from SO2 probably made a negligible contribution to the growth of >10 nm new particles. 相似文献
103.
The oxidation of SO2 is commonly regarded as a major driver for new particle formation (NPF) in the atmosphere. In this study, we explored the connection between measured mixing ratio of SO2 and observed long-term (duration > 3 hr) and short-term (duration < 1.5 hr) NPF events at a semi-urban site in Toronto. Apparent NPF rates (J30) showed a moderate correlation with the concentration of sulfuric acid ([H2SO4]) calculated from the measured mixing ratio of SO2 in long-term NPF events and some short-term NPF events (Category I) (R2 = 0.66). The exponent in the fitting line of J30 ~ [H2SO4]n in these events was 1.6. It was also found that SO2 mixing ratios varied a lot during long-term NPF events, leading to a significant variation of new particle counts. In the SO2-unexplained short-term NPF events (Category II), analysis showed that new particles were formed aloft and then mixed down to the ground level. Further calculation results showed that sulfuric acid oxidized from SO2 probably made a negligible contribution to the growth of > 10 nm new particles. 相似文献
104.
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107.
S. H. Park E. J. Kim T. H. Yun J. H. Lee C. K. Kim Y. H. Seo S. A. Oh S. S. Choi S. J. Cho M. S. Kim G. Y. Han M. Y. Kim H. S. Jeong D. S. Cheon H. S. Kim 《Food and environmental virology》2010,2(2):69-73
Waterborne outbreaks of enteric viruses are a major public health concern. The present study has been carried out to assess the presence of enteric viruses responsible for human acute gastroenteritis (AGE) in groundwater intended for drinking and produce washing. In total, 62 samples from groundwater for drinking and produce washing collected from Dec 2007 to Dec 2008 in Seoul were tested for enteric viruses using conventional RT–PCR, ELISA, and real-time RT–PCR. Our results showed that enteric viruses were detected in 7 (8.8%) groundwater samples. Rotaviruses were detected in 3 (4.8%) of the samples by ELISA; human adenoviruses were detected in 2 (3.2%) of the samples by ELISA; and nested RT–PCR detected noroviruses in 2 (3.2%) of the samples. In one of the groundwater sample, the norovirus RNA was detected by conventional RT–PCR which was confirmed positive by real-time RT–PCR. Additionally, real-time RT–PCR successfully detected norovirus RNA in five out of 62 water samples (8.1%). The data demonstrate that real-time RT–PCR will be useful as a rapid and sensitive method for detecting norovirus in water samples. Phylogenetic analysis revealed that the noroviruses detected in two of the groundwater samples belonged to GII-4. These studies can provide important information for the prevalence of enteric viruses in Korean groundwater. 相似文献
108.
Kyong Whan Moon Eun Hae Huh Ho Chul Jeong 《Environmental monitoring and assessment》2014,186(4):2111-2120
The aims of the present study were to determine the levels of bioaerosols including airborne culturable bacteria (total suspended bacteria, Gram-positive bacteria, Staphylococcus, Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), and Gram-negative bacteria), fungi, endotoxin, and viruses (influenza A, influenza B, respiratory syncytial virus types A/B, parainfluenza virus types 1/2/3, metapnemovirus, and adenovirus) and their seasonal variations in indoor air of residential apartments. Of the total suspended bacteria cultured in an indoor environment, Staphylococcus was dominant and occupied 49.0 to 61.3 % of indoor air. Among Staphylococcus, S. aureus were detected in 100 % of households' indoor air ranging from 4 to 140 CFU/m3, and 66 % of households were positive for MRSA ranging from 2 to 80 CFU/m3. Staphylococcus and S. aureus concentrations correlated with indoor temperature (adjusted β: 0.4440 and 0.403, p?<?0.0001). Among respiratory viruses, adenovirus was detected in 14 (14 %) samples and influenza A virus was detected in 3 (3 %) samples regarding the indoor air of apartments. Adenovirus concentrations were generally higher in winter (mean concentration was 2,106 copies/m3) than in spring (mean concentration was 173 copies/m3), with concentrations ranging between 12 and 560 copies/m3. Also, a strong negative correlation between adenovirus concentrations and relative humidity in indoor air was observed (r?=??0.808, p?<?0.01). Furthermore, temperature also negatively correlated with adenovirus concentrations (r?=??0.559, p?<?0.05). 相似文献
109.
Jeong Huy Ko Yeon-Ho Kwak Kyung-Seun Yoo Jong-Ki Jeon Sung Hoon Park Young-Kwon Park 《Journal of Material Cycles and Waste Management》2011,13(3):173-179
Experiments were performed in order to investigate the possibility for the development of catalysts for low-temperature selective
catalytic reduction (SCR) using municipal waste char and RDF byproduct. Physical and chemical activations, using water, and
HCl and KOH, were employed to increase the catalytic activities. The characteristics of the activated catalysts were investigated
using N2 adsorption–desorption and FT-IR. The catalysts activated chemically using basic treatment showed higher NO
x
removal efficiencies than those activated physically or chemically using acidic treatment. The de-NO
x
performance of the activated catalysts was dependent on the chemical properties, such as oxygen functional groups as well
as physical properties, such as specific surface area and pore volume. In order to investigate the effect of MnO
x
, which has been reported to be efficient for the removal of NO
x
in low-temperature SCR processes, the chemically activated catalyst was impregnated with manganese. The Mn-impregnated catalyst
had the highest NO
x
conversion at all of the temperatures tested in this study. 相似文献
110.
Min Park Sung Hoon Shim Sang Hyun Jeong Kwang-Joong Oh 《Journal of the Air & Waste Management Association (1995)》2017,67(4):402-411
The nitrogen oxides (NOx) reduction technology by combustion modification which has economic benefits as a method of controlling NOx emitted in the combustion process, has recently been receiving a lot of attention. Especially, the moderate or intense low oxygen dilution (MILD) combustion which applied high temperature flue gas recirculation has been confirmed for its effectiveness with regard to solid fuel as well. MILD combustion is affected by the flue gas recirculation ratio and the composition of recirculation gas, so its NOx reduction efficiency is determined by them. In order to investigate the influence of factors which determine the reduction efficiency of NOx in MILD coal combustion, this study changed the flow rate and concentration of nitrogen (N2), carbon dioxide (CO2) and steam (H2O) which simulate the recirculation gas during the MILD coal combustion using our lab-scale drop tube furnace and performed the combustion experiment. As a result, its influence by the composition of recirculation gas was insignificant and it was shown that flue gas recirculation ratio influences the change of NOx concentration greatly. Implications: We investigated the influence of factors determining the nitrogen oxides (NOx) reduction efficiency in MILD coal combustion, which applied high-temperature flue gas recirculation. Using a lab-scale drop tube furnace and simulated recirculation gas, we conducted combustion testing changing the recirculation gas conditions. We found that the flue gas recirculation ratio influences the reduction of NOx emissions the most. 相似文献