A design of experiments for statistically predicting risk of adverse health effects on drivers exposed to vertical vibrations

An injury risk factor (IRF), which indicates the risk of adverse health effect to lumbar rachis arising from mechanical vibrations, is developed. Experiments have been conducted that consider acceleration levels at the seat of drivers, posture, morphology, density, damping rate and body mass as independent variables. A parametric finite-element model of the lumbar rachis has been generated. It is shown that the IRF increases with ageing and an IRF of 30% is proposed as a threshold for fatigue purposes. This level is reached if a peak acceleration level greater than 3 m/s2 is applied to a light (55 kg) and an old driver with a low bone density and a damping rate of 20%. This vibration threshold must be reduced to 2.7 m/s2 if the driver?s weight increases to 75 kg and to 2 m/s2 if the driver is heavy (98 kg).     

Microbial and chemical markers: runoff transfer in animal manure-amended soils

Fecal contamination of water resources is evaluated by the enumeration of the fecal coliforms and Enterococci. However, the enumeration of these indicators does not allow us to differentiate between the sources of fecal contamination. Therefore, it is important to use alternative indicators of fecal contamination to identify livestock contamination in surface waters. The concentration of fecal indicators (, enteroccoci, and F-specific bacteriophages), microbiological markers (Rum-2-bac, Pig-2-bac, and ), and chemical fingerprints (sterols and stanols and other chemical compounds analyzed by 3D-fluorescence excitation-matrix spectroscopy) were determined in runoff waters generated by an artificial rainfall simulator. Three replicate plot experiments were conducted with swine slurry and cattle manure at agronomic nitrogen application rates. Low amounts of bacterial indicators (1.9-4.7%) are released in runoff water from swine-slurry-amended soils, whereas greater amounts (1.1-28.3%) of these indicators are released in runoff water from cattle-manure-amended soils. Microbial and chemical markers from animal manure were transferred to runoff water, allowing discrimination between swine and cattle fecal contamination in the environment via runoff after manure spreading. Host-specific bacterial and chemical markers were quantified for the first time in runoff waters samples after the experimental spreading of swine slurry or cattle manure.     

Cost analysis in laccase production

In this paper the cost of producing the enzyme laccase by the white-rot fungus Trametes pubescens under both submerged (SmF) and solid-state fermentation (SSF) conditions was studied. The fungus was cultured using more than 45 culture medium compositions. The cost of production was estimated by analyzing the cost of the culture medium, the cost of equipment and the operating costs. The cost of the culture medium represented, in all cases, the highest contribution to the total cost, while, the cost of equipment was significantly low, representing less than 2% of the total costs. The cultivation under SSF conditions presented a final cost 50-fold lower than the one obtained when culturing under SmF conditions at flask scale. In addition, the laccase production under SSF conditions in tray bioreactors reduced the final cost 4-fold compared to the one obtained under SSF conditions at flask scale, obtaining a final price of 0.04 cent €/U.     

Do birds and beetles show similar responses to urbanization?

To date, the vast majority of studies in urban areas have been carried out on birds, yet it is not known whether the responses of birds to urbanization are congruent with those of other taxa. In this paper, we compared the responses of breeding birds and carabid beetles to urbanization, specifically asking whether the emerging generalizations of the effects of extreme levels of urbanization on birds (declines in total species richness and the richness of specialist species, increases in total abundance and the abundances of native generalist and introduced species, and community simplification, including increasing similarity) could also be applied to ground beetles. We also directly tested for congruence between birds and ground beetles using correlations between variables describing bird and beetle community structure and correlations between bird and beetle distance matrices describing community dissimilarity between pairs of sampling locations. Breeding bird and carabid beetle community data were collected in Ottawa, Ontario, and Gatineau, Quebec, Canada, in two groups of sites: developed sites representing the predictor variable within-site housing density, and forested sites adjacent to development representing the predictor variable neighboring housing density (each site was 0.25 km2). Breeding birds and carabid beetles do not respond similarly to increasing within-site housing density but do exhibit some similar responses to increasing neighboring housing density. Birds displayed strong declines in diversity, compositional changes, and community simplification in response to increasing within-site housing density. Forest and introduced species of birds and beetles responded similarly to increasing housing density within a site, but responses of overall diversity and open-habitat species richness and patterns of community simplification differed between birds and beetles. Increasing neighboring housing density resulted in increases in the abundances of introduced birds and introduced beetles and similar patterns of community simplification in both taxa. To better understand and mitigate the effects of urbanization on biodiversity, we suggest that, in addition to the responses of birds, future research should focus on the responses of other taxa in the urban matrix.     

Temporal and spatial changes in plant dune diversity in urban resorts

We investigated the temporal and spatial changes in the floristic composition and abundance in sand dunes along a coastal strip in the province of Buenos Aires (Argentina). Firstly, we analysed changes over a span of 70 years, comparing 41 of our own inventories carried out in 14 beach resorts in 2005–2006 with 18 inventories made in the first half of the XXth century (1930–1940) in the same study area. We grouped sampled plots into four categories for comparison, taking into account the time period (1930–1940 or 2005–2006) and location at each end of the surveyed coastal strip in the northern or southern ‘tourist’ sectors. Secondly, we analysed the effect of afforestation with exotic trees on extant sand dune vegetation for the period 2005–2006. A total of 74 plant inventories were carried out in afforested and natural dunes at the same resorts. In both comparisons we contrasted plant richness, percentage plant cover, similarity and diversity. A cluster analysis was used to classify categories taking into account plant abundance and composition. Significant differences in total diversity were observed between sampling years, suggesting an increase in diversity at the present time due to urbanization and recreation related activities. Nevertheless, no single exotic plant species was clearly dominant across the sampled sites, suggesting that these coastal areas are, up to now, resistant to alien invasion. Diversity indexes discriminated by plant groups indicating different habitat conditions and exotics were shown to be more sensitive to existing changes than to total richness and cover.     

A preliminary assessment of levels of antifouling booster biocides in harbours and marinas of the island of Gran Canaria, using SPE-HPLC

An analytical procedure was developed to allow for the study of the levels of concentration of biocides in the various different ports on the island of Gran Canaria, The Canary Islands. The analytes studied were extracted from water samples using solid-phase extraction and then determined by high-performance liquid chromatography using a diode array detector. The relative standard deviations of the developed procedure were under 12%. Recoveries over 85% and limits of detection between 0.007 and 0.4 μg/L were obtained for all the analytes. The method was applied to the analysis of sea water samples from the ports and marinas on the island of Gran Canaria.     

Assessment of trace metals pollution in estuarine sediments using SEM-AVS and ERM?CERL predictions

In this paper a PSIAC-based multi-parameter fuzzy pattern recognition (MPFPR) model is proposed and applied for classifying and ranking the potential soil erosion (PSE). In this approach, standard value matrix is used to define the membership degrees of each catchment to each class and the feature values are used for alternative ranking. The characteristic of PSE for each class is expressed by linguistic variables. The proposed method is straightforward, easy to understand, very practical, and its results may easily be interpreted. To assess the performance of the model, the results of PSIAC MPFPR and original PSIAC method are interpreted and compared with the observed data. It is shown that the proposed approach reflects the fuzzy nature of the soil erosion more efficiently and is quite robust for application in real world cases.     

Life cycle assessment of solar photo-Fenton and solar photoelectro-Fenton processes used for the degradation of aqueous α-methylphenylglycine

A comparative Life Cycle Assessment (LCA) of solar photo-Fenton and solar photoelectro-Fenton, two solar-driven advanced oxidation processes (AOPs) devoted to the removal of non-biodegradable pollutants in water, is performed. The study is based on the removal, at laboratory scale, of the amino acid α-methylphenylglycine, a good example of soluble and non-biodegradable target pollutant. The system under study includes chemicals, electricity, transport of all raw materials to the plant site, and the generation of emissions, but it does not take into account the impact of the infrastructure needed to build a hypothetical solar plant. Nine environmental impact categories are included in the LCA: global warming potential, ozone depletion potential, aquatic eutrophication potential, acidification potential, human toxicity potential, photochemical ozone formation potential, fresh water aquatic ecotoxicity potential, marine aquatic ecotoxicity potential, and terrestrial ecotoxicity potential and abiotic resource depletion potential. Although previous experimental results show that both AOPs are able to efficiently degrade the pollutant, the LCA indicates that solar-driven photo-Fenton is the most environmentally friendly alternative, mainly because the use of electricity in solar photoelectro-Fenton experiments involves high environmental impacts.     

Combined use of Nassarius reticulatus imposex and statolith age determination for tracking temporal evolution of TBT pollution in the NW Portuguese continental shelf

Spatial and temporal trends of tributyltin (TBT) pollution on the northern continental shelf of Portugal (5-34 m depth) were assessed using Nassarius reticulatus as a bioindicator. Imposex levels were determined in 2006 and 2010, and integrated with age readings from statoliths for a better chronological analysis of the data. Females affected with imposex were detected in 84% of the sites surveyed in 2006, with levels higher than the Ecological Quality Objective (EcoQO) set by the OSPAR Commission for N. reticulatus (VDSI < 0.3) in approximately half of those sites. In 2010, imposex was only detected in 20% of the surveyed sites and, when detected, the VDSI were lower than the EcoQO, except for one location. The comparison between the current results and the levels previously reported for 2004 and 2005 in the same offshore area evidences a clear decline of imposex in 2010 that confirms the efficacy of Regulation (EC) No. 782/2003 and the IMO AFS-Convention (entered into force in 2003 and 2008, respectively). Moreover, the age of the whelks used in the monitoring is estimated in 3-5 years, indicating that actual TBT water contamination in 2010 may in fact be lower than that extrapolated from the imposex levels. We conclude that the legislation was indeed very effective in reducing TBT pollution in the NW Portuguese continental shelf, not only in terms of the magnitude but also in terms of fast field repercussion.