Detecting diclofenac in livestock carcasses in India with an ELISA: a tool to prevent widespread vulture poisoning

Diclofenac, a non-steroidal anti-inflammatory drug (NSAID), has caused catastrophic vulture declines across the Indian sub-continent. Here, an indirect ELISA is used to detect and quantify diclofenac in 1251 liver samples from livestock carcasses collected across India between August 2007 and June 2008, one to two years after a ban on diclofenac manufacture and distribution for veterinary use was implemented. The ELISAs applicability was authenticated with independent data obtained using LC-ESI/MS. Of 1251 samples, 1150 (91.9%) were negative for diclofenac using both methods, and 60 (4.8%) were positive at 10-4348 and 10-4441 μg kg(-1) when analysed by ELISA and LC-ESI/MS, respectively. The residue level relationship in the 60 positive samples was highly significant (p < 0.001, r(2) = 0.644). Data suggest that this immunological assay could be used not only for cost effective sample screening, but also for residue level semi-quantification.     

In situ stabilization of trace metals in a copper-contaminated soil using P-spiked Linz-Donawitz slag

Purpose  

A former wood exploitation revealing high Cu and As concentration of the soils served as a case study for assisted phytoextraction.     

Plasma esterases in the tegu lizard Tupinambis merianae (Reptilia, Teiidae): impact of developmental stage, sex, and organophosphorus in vitro exposure

Purpose  

In this study, we determined normal serum butyrylcholinesterase (BChE) and carboxylesterase (CbE) activities in Tupinambis merianae in order to obtain reference values for organophosphorus pesticide monitoring.     

Kinetic study of γ-hexabromocyclododecane orally given to laying hens (Gallus domesticus)

Introduction  

High concentrations of hexabromocyclododecane (HBCD) sometimes recorded in free-range hens' eggs are thought to be due to soil ingestion. Of the three stereoisomers of HBCD (α-, β-, and γ-HBCD), γ-HBCD is the main component in the commercial mixture, as well as in environmental matrices, whereas the isomer profile is α-dominated in biota. In fish and in mammals, this shift is thought to be due to a rapid elimination of γ-HBCD and to its bioisomerization to the more persistent α-HBCD. The aim of the current controlled study was to better understand the fate of ingested HBCD in laying hens. The isomer profile in soil being γ-dominated, excretion kinetics of γ-HBCD into egg yolk, and accumulation in liver and in abdominal fat were investigated.     

Exposure to toxic waste containing high concentrations of hydrogen sulphide illegally dumped in Abidjan, C?te d’Ivoire

Introduction

On August 2006, a cargo ship illegally dumped 500?t of toxic waste containing high concentrations of hydrogen sulphide in numerous sites across Abidjan. Thousands of people became ill. Seventeen deaths were associated with toxic waste exposure.

Materials and methods

This study reports on environmental and health problems associated with the incident. A cross-sectional transect study was conducted in five waste dumping site areas.

Results

Of the households, 62.1% (n?=?502) were exposed to the effects of the pollutants and 51.1% of the interviewed people (n?=?2,368) in these households showed signs of poisoning. Most important symptoms were cough (37.1%), asthenia (33.1%), pruritus (29.9%) and nausea (29.1%).

Discussion

The health effects showed different frequencies in the five waste impact sites. Among the poisoned persons, 21.1% (n?=?532) presented symptoms on the survey day (i.e., 4?months after incident). Transect sampling allowed to determine a radius of vulnerability to exposure of up to 3?km from the point of toxic waste disposal.

Conclusion

The area of higher vulnerability is influenced by various environmental factors, such as size and severity of pollution site, duration of toxic waste pollution on the impact site and locally climatic conditions. The surveillance of effects on environment and human health is warranted to monitor the development.     

Apple snails and their endosymbionts bioconcentrate heavy metals and uranium from contaminated drinking water

Purpose

The differential ability of apple snail tissues, endosymbionts, and eggs to bioaccumulate several metals (Sb, As, Ba, Br, Zn, Cr, Fe, Hg, Se, and U) was investigated.

Methods

Metal concentrations were determined by neutron activation analysis in several tissues, endosymbionts, and eggs from mature apple snails cultured in either drinking water or reconstituted water (prepared with American Society for Testing and Materials type I water).

Results

The highest bioconcentration factors (BCFs) in the midgut gland were found for Ba, Zn, Se, As, U, Br, and Hg (in decreasing order), while the highest in the kidney were for Ba, Br, and Hg. The foot showed the highest BCFs for Ba, Hg, Br, and Se (in decreasing order). Calcified tissues (uterus, shell) and eggs showed low BCFs, except for Ba. Both C corpuscles and gland tissue showed statistically higher BCFs than K corpuscles for Ba, Fe, U, Br, and Sb. The concentration of most of the studied elements was significantly lower in tissues and endosymbionts obtained from snails cultured in reconstituted water instead of drinking water. Snails cultured in reconstituted water and then exposed or not to Hg, As, and U (at the maximum contaminant level allowed by the US Environmental Protection Agency) also resulted in high levels accumulated in midgut gland, endosymbionts and kidney.

Conclusions

Our findings suggest that the midgut gland (and the symbionts contained therein), the kidney, and the foot of Pomacea canaliculata may be useful bioindicators of Hg, As and U pollution in freshwater bodies and that the unrestricted use of ampullariid snails as human and animal food must be considered with caution..     

Assessing and forecasting the impacts of global change on Mediterranean rivers. The SCARCE Consolider project on Iberian basins

Introduction

The Consolider-Ingenio 2010 project SCARCE, with the full title ??Assessing and predicting effects on water quantity and quality in Iberian Rivers caused by global change?? aims to examine and predict the relevance of global change on water availability, water quality, and ecosystem services in Mediterranean river basins of the Iberian Peninsula, as well as their socio-economic impacts. Starting in December 2009, it brought together a multidisciplinary team of 11 partner Spanish institutions, as well as the active involvement of water authorities, river basin managers, and other relevant agents as stakeholders.

Methods

The study areas are the Llobregat, Ebro, Jucar, and Guadalquivir river basins. These basins have been included in previous studies and projects, the majority of whom considered some of the aspects included in SCARCE but individually. Historical data will be used as a starting point of the project but also to obtain longer time series. The main added value of SCARCE project is the inclusion of scientific disciplines ranging from hydrology, geomorphology, ecology, chemistry, and ecotoxicology, to engineering, modeling, and economy, in an unprecedented effort in the Mediterranean area. The project performs data mining, field, and lab research as well as modeling and upscaling of the findings to apply them to the entire river basin.

Results

Scales ranging from the laboratory to river basins are addressed with the potential to help improve river basin management. The project emphasizes, thus, linking basic research and management practices in a single framework. In fact, one of the main objectives of SCARCE is to act as a bridge between the scientific and the management and to transform research results on management keys and tools for improving the River Basin Management Plans. Here, we outline the general structure of the project and the activities conducted within the ten Work Packages of SCARCE.     

Meta-analysis of environmental contamination by alkylphenols

Alkylphenols and alkylphenol ethoxylates (APE) are toxics classified as endocrine-disrupting compounds; they are used in detergents, paints, herbicides, pesticides, emulsifiers, wetting and dispersing agents, antistatic agents, demulsifiers, and solubilizers. Many studies have reported the occurrence of alkylphenols in different environmental matrices, though none of these studies have yet to establish a comprehensive overview of such compounds in the water cycle within an urban environment. This review summarizes APE concentrations for all environmental media throughout the water cycle, from the atmosphere to receiving waters. Once the occurrence of compounds has been assessed for each environmental compartment (urban wastewater, wastewater treatment plants [WWTP], atmosphere, and the natural environment), data are examined in order to understand the fate of APE in the environment and establish their geographical and historical trends. From this database, it is clear that the environment in Europe is much more contaminated by APE compared to North America and developing countries, although these APE levels have been decreasing in the last decade. APE concentrations in the WWTP effluent of developed countries have decreased by a factor of 100 over the past 30?years. This study is aimed at identifying both the correlations existing between environmental compartments and the processes that influence the fate and transport of these contaminants in the environment. In industrial countries, the concentrations observed in waterways now represent the background level of contamination, which provides evidence of a past diffuse pollution in these countries, whereas sediment analyses conducted in developing countries show an increase in APE content over the last several years. Finally, similar trends have been observed in samples drawn from Europe and North America.     

Treatment of bleach pulp mill effluent by MF-MBR

The types of treatment most commonly used by pulp mills are biological treatments in combination with sedimentation or coagulation/flocculation as pretreatment. The main issues faced by these types of treatment are low efficiency in the removal of organic matter and the loss of aggregate value for the recovered fiber. Therefore, this study aims to evaluate the use of microfiltration (MF) combined with a membrane bioreactor (MBR) for the treatment of bleach pulp mill effluents. The results showed that the use of the MF-MBR system was an excellent alternative for the treatment of bleach pulp mill effluents with an average COD removal of 95%. The microfiltration allowed the recovery of fibers, which can be returned to the production process without losing economic value, and produced a better quality effluent for further treatment in the MBR. The MBR presented high efficiency removal of organic matter.     

Evaluating the degradation of the herbicides picloram and 2,4-D in a compartmentalized reactive biobarrier with internal liquid recirculation

Tordon is a widely used herbicide formulation of 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-amino-3,5,6-trichloropicolinic acid (picloram), and it is considered a toxic herbicide. The purposes of this work were to assess the feasibility of a microbial consortium inoculated in a lab-scale compartmentalized biobarrier, to remove these herbicides, and isolate, identify, and evaluate their predominant microbial constituents. Volumetric loading rates of herbicides ranging from 31.2 to 143.9 g m^?3 day^?1, for 2,4-D, and 12.8 to 59.3 g m^?3 day^?1 for picloram were probed; however, the top operational limit of the biobarrier, detected by a decay in the removal efficiency, was not reached. At the highest loading rates probed, high average removal efficiencies of 2,4-D, 99.56?±?0.44; picloram, 94.58?±?2.62; and chemical oxygen demand (COD), 89.42?±?3.68, were obtained. It was found that the lab-scale biofilm reactor efficiently removed both herbicides at dilution rates ranging from 0.92 to 4.23 day^?1, corresponding to hydraulic retention times from 1.087 to 0.236 days. On the other hand, few microbial strains able to degrade picloram are reported in the literature. In this work, three of the nine bacterial strains isolated cometabolically degrade picloram. They were identified as Hydrocarboniphaga sp., Tsukamurella sp., and Cupriavidus sp.