Multilingual education of students on a global scale and perspective—international networking on the example of bioindication and biomonitoring (B&B technologies)

Living or formerly living organisms are being used to obtain information on the quality of the general health status of our environment by bioindication and biomonitoring methods for many decades. Thus, different roads toward this common scientific goal were developed by a lot of different international research groups. Global cooperation in between various scientific teams throughout the world has produced common ideas, scientific definitions, and highly innovative results of this extremely attractive working field. The transdisciplinary approach of different and multifaceted scientific areas—starting from biology, analytical chemistry, via health physics, up to social and economic issues—have surpassed mental barriers of individual scientists, so that “production” of straightforward common results related to the influence of material and immaterial environmental factors to the well-being of organisms and human life has now reached the forefront of international thinking. For the further sustainable development of our common scientific “hobby” of bioindication and biomonitoring, highest personal energy has to be given by us, being teachers to our students and to convince strategically decision makers as politicians to invest (financially) into the development of education and research of this innovative technique. Young people have to be intensively convinced on the “meaning” of our scientific doing, e.g., by extended forms of education. One example of multilingual education of students on a global scale and perspective is given here, which we started about 3 years ago.     

Investigation of chloramine-T impact on crayfish Astacus leptodactylus (Esch., 1823) cardiac activity

The crayfish play an essential role in the biomonitoring and may reflect ambient water quality through the biochemical, behavioural and physiological reactions. To assess whether narrow-clawed crayfish Astacus leptodactylus can respond by heart rate changes to presence in water of such biocide as chloramine-T, adult males were exposed to its low (2 and 5 mg L^?1), moderate (10 mg L^?1, commonly used in industry and aquaculture) and exceeded (20 and 50 mg L^?1) concentrations. In addition, a physical stress test evaluated energy expenditure following the chemical trials. Three key reactions (cardiac initial, first-hour and daily prolonged exposure) were discussed with particular focus on crayfish initial reaction as the most meaningful in on-line water quality biomonitoring. After short-term exposure to both chloramine-T concentrations, crayfish were found to respond rapidly, within 2–5 min. According to heart rate changes, the 1-h exposure did not adversely affect crayfish at either concentration, as well as during daily exposure to 10 mg L^?1. As assessed by the heart rate, the 24-h exposure to 50 mg L^?1 of chloramine-T was toxic for crayfish and led to substantial loss of energy that became apparent during subsequently conducted physical stress. The results supported a hypothesis that crayfish vital functions are connected with environment they inhabit closely enough to serve as biological monitors. Crayfish were tolerant to short-term chloramine-T exposure, while rapid crayfish reaction to an increased chemical level indicated their high sensitivity, an essential attribute of real-time environmental assessment.     

Assessment of air pollution by mercury in South African provinces using lichens Parmelia caperata as bioindicators

Environmental Geochemistry and Health - Large-scale assessment of atmospheric air pollution by mercury (Hg) using lichen Parmelia caperata as biological indicator was undertaken using samples from...     

In vitro activity of antimicrobial agents against Pseudomonas tolaasii,pathogen of cultivated button mushroom

In vitro antibacterial activity tests of seven biofungicides (Ekstrasol, Bisolbisan, Bisolbifit, Serenade, Sonata, Timorex, F-Stop) and two disinfectants (colloidal silver alone and in combination with hydrogen peroxide) against the Pseudomonas tolaasii strain (NS3B6) were carried out by the disc-diffusion, broth microdilution and broth macrodilution method. Biofungicides tested in this study did not exhibit any antimicrobial activity in neither one of the methods used. Disc diffusion method revealed high sensitivity of the tested P. tolaasii strain to Ecocute based on colloidal silver and hydrogen peroxide. Both microdilution and macrodilution methods identified the same MICs and MBCs of Ecocute (0.19 mg/L) for P. tolaasii strain. MICs and MBCs values of silver alone were much higher (10 mg/L) compared to silver in combination with hydrogen peroxide.     

Analysis of the toxicogenomic effects of exposure to persistent organic pollutants (POPs) in Slovakian girls: correlations between gene expression and disease risk

The chemical composition of persistent organic pollutants (POPs) in the environment is not uniform throughout the world, and these contaminants contain many structurally different lipophilic compounds. In a well-defined study cohort in the Slovak Republic, the POP chemicals present in the peripheral blood of exposed children were chemically analyzed. The chemical analysis data revealed that the relative concentration and profile of structurally different organic pollutants, including polychlorinated biphenyls (PCBs), 2,2′-bis(4-chlorophenyl)-1,1-dichloroethylene (p,p′-DDE), 2,2′-bis(4-chlorophenyl)-1,1,1-trichloro-ethane (p,p′-DDT), hexachlorobenzene (HCB) and β-hexachlorocyclohexane (β-HCH), may vary from individual to individual, even within the same exposure area. These chemicals can be broadly classified into two groups. The first group, the PCB congeners, primarily originated from industrial compounds and their byproducts. The second group of compounds originated from or was commonly used in the agricultural sector (e.g., DDT, HCB). The objective of this study was to examine the effects of the two POP exposure profiles on gene expression. For the study population, we selected pre-pubertal girls (mean age of 46.2 ± 1.4 months) with high POP concentrations in their blood (> 75% tile of total POP) and classified them in the high ‘PCB’ group when the total PCB concentration was significantly higher than the total concentration of other POP components and in the ‘Other Than PCB’ (OTP) group, when the total PCB concentration was significantly lower than the concentration of the other major POP constituents. A matched control group of girls (< 25% tile of total POP) was selected for comparison purpose (n = 5 per group). Our aims were to determine whether there were any common effects of high POP exposure at a toxicogenomic level and to investigate how exposure may affect physiological functions of the children in two different exposure scenarios. Global gene expression analysis using a microarray (Affymetrix Gene Chip Human genome U133 Plus 2.0 Array) platform was conducted on the total RNA of peripheral blood mononuclear cells from the girls. The results were analyzed by Partek GS, Louis, MI, which identified twelve genes (ATAD2B, BIVM, CD96, CXorf39, CYTH1 ETNK1, FAM13A, HIRA, INO80B, ODG1, RAD23B, and TSGA14) and two unidentified probe sets, as regulated differentially in both the PCB and OTP groups against the control group. The qRT-PCR method was used to validate the microarray results. The Ingenuity Pathway Analysis (IPA) software package identified the possible molecular impairments and disease risks associated with each gene set. Connective tissue disorders, genetic disorders, skeletal muscular disorders and neurological diseases were associated with the 12 common genes. The data therefore identified the potential molecular effects of POP exposure on a genomic level. This report underscores the importance of further study to validate the results in a random population and to evaluate the use of the identified genes as biomarkers for POP exposure.     

Geophysical investigations for evaluation of environmental pollution in a mine tailings area

Sulfide-containing mill wastes of the Komsomolsk ore processing plant situated in the Kemerovo region (Russia) were examined in 2013–2015. Multipurpose studies of the mine tailings determined the composition of waste, pore water, mine drainages, and affected groundwater. Electrical resistivity tomography was used to trace the geoelectric zoning of the waste samples. Layers with low resistivity indicated areas with pore spaces filled with highly mineralized solutions with Fe, Cu, Zn, Cd, As, and Sb at total concentrations of up to 50 g/L. Anomalous zones can be specified as ‘geochemical barriers’ – specific layers where the mobility of the elements is reduced due to pH conditions, redox potential, and Fe(III) hydroxide precipitation. The zones of increased conductivity in oxidized mine tailings indicated local areas with high acid production potential and coexisting acidic pore solution. In non-oxidized tailings, high conductivity of the mineral skeleton was observed. There was a migration of drainage outside the tailings, its direction monitored by geophysical data. Chemical analysis confirmed that the concentrations of As in groundwater samples were higher than the maximum permissible concentration.     

An evaluation of adaptation options for climate change impacts on agriculture in Kazakhstan

Agriculture in Kazakhstan is sensitive to climate, and wheat yields could be reduced up to 70% under climate change. With the transition from a socialist economy to a free market economy, decisions are being made now that will affect Kazakhstan's ability to cope with climate change. A team of Kazakh and American researchers examined the cost-effectiveness and barriers to implementations of adaptation options for climate change. Twelve adaptation options that increase flexibility to respond to climate change were identified using a screening matrix. Four options, forecasting pest outbreaks, developing regional centers for preserving genetic diversity of seeds, supporting a transition to a free market, and reducing soil erosion through the use of changed farming practices, were examined. The Adaptation Decision Matrix (ADM) was then applied to estimate benefits using expert judgment (using an arbitrary numerical scale, not monetary values) and benefits estimates were compared to costs to determine cost-effectiveness. The ADM uses subjective measures of how well adaptation options meet policy objectives. Controlling soil erosion was estimated to have the highest benefits, but the high costs of implementation appears to make it relatively cost-ineffective. Supporting a transition to a free market was ranked as the most cost-effective measure, with regional centers second. However, use of different scales to quantify benefits or different weights can result in regional centers being more cost-effective than the transition to a free market. Regional centers was also judged to have fewer barriers to implementation than a transition to a free market. These results will be incorporated in Kazakhstan's National Action Plan. The ADM and other tools are relatively easy to apply, but are quite subjective and difficult to evaluate. The tools can be quite useful by decision makers to analyze advantages and disadvantages between different adaptation options, but should be supplemented with additional, particularly quantitative analysis.     

Toxicity of fungicides with different modes of action to Cladobotryum dendroides and Agaricus bisporus

Isolates of Cladobotryum dendroides from Serbian mushroom farms and Agaricus bisporus F56 were tested for sensitivity to selected fungicides in vitro. Chlorothalonil was the most toxic fungicide to C. dendroides isolates (EC₅₀ values were below 1.68 mg L^{? 1}). Trifloxystrobin and kresoxim-methyl were not effective in growth inhibition of C. dendroides isolates (EC₅₀ values exceeded 300 mg L^{? 1}). Metalaxyl-M+mancozeb was the most toxic fungicide to strain F56 of A. bisporus, and iprodione the least toxic. The fungicide selectivity indexes for both C. dendroides and A. bisporus indicated that iprodione, chlorothalonil, captan and metalaxyl-M+mancozeb had satisfactory selective fungitoxicity. Iprodione had the best selectivity to both the pathogen and the host, although inferior than prochloraz manganese and carbendazim, fungicides officially recommended for mushroom cultivation in European Union (EU) countries.