Important sequence for overexpression of NADH oxidase gene from Thermus thermophilus HB8 in Escherichia coli

Enzymes fixed on the electrode of biosensor are gradually inactivated and the electrode is discarded after using several times. In order to prepare the stable biosensor, we try to use a stable enzyme from extreme thermophilic bacteria, Thermus thermophilus HB8. It is very important that a stable enzyme from T. thermophilus HB8 is overproduced in Escherichia coli, for the purpose of enough supply of enzyme. Thereby, we determined the important sequence for overexpression of NADH oxidase (nox) gene from T. thermophilus HB8 in E. coli. As a result, it is revealed that ten nucleotides sequence, GAAATTAACT, in the upstream of start codon of nox gene was important for its overexpression in E. coli.     

Mechanical behavior of inert waste landfills under seismic condition

Journal of Material Cycles and Waste Management - Mechanical properties and slope stability of inert waste landfills under seismic condition were studied with three different approaches: in-situ...     

Effects of adding slags on strength and leaching properties of soft soil

Journal of Material Cycles and Waste Management - Dredged soils are typically mixed with additives to improve their mechanical properties and reduce the risks of leaching toxic elements. An...     

The effect of predation risk on post-copulatory sexual selection in the Japanese pygmy squid

Behavioral Ecology and Sociobiology - The pace-of-life syndrome (POLS) hypothesis predicts that behavior and physiology covary with life history. Evidence for such covariation is contradictory,...     

Development of a Model for Predicting the Volatilization Flux from Unsaturated Soil Contaminated by Volatile Chemical Substances

Environmental Modeling & Assessment - This work developed a model for predicting the volatilization flux from the unsaturated soil contaminated by volatile chemical substances (VCSs) such as...     

Material reuse and recycling in construction works in Japan

Journal of Material Cycles and Waste Management - The status of material reuse and recycling in Japan is reviewed with an emphasis on the efforts by the national government. First, the national...     

Short-duration exposure of 3-µm polystyrene microplastics affected morphology and physiology of watermilfoil (sp. roraima)

Environmental Science and Pollution Research - Microplastics are one of the most widely discussed environmental issues worldwide. Several studies have shown the effect of microplastic exposure on...