Planning and Conducting Sampling Surveys of Hazardous Wastes at Industrial Facilities

Many organizations and their consultants are involved in sampling programs to characterize waste streams and materials at industrial facilities. Due to financial limitations, calendar deadlines, or other practical restrictions, a short-term, survey-type sampling plan (“sampling survey”) may need to he implemented. Survey results can he used to determine if the wastes are hazardous or if they are being managed in accordance with applicable regulations. This paper brings together practical experience and diverse information to identify the major concerns in conducting sampling surveys and to suggest methods of approaching these concerns. The paper addresses sampling in time and space, “correct” sampling to minimize bias, selecting equipment to minimize contamination, sample handling, and quality assurance/quality control. Even a quick sampling survey can be a high quality effort with a careful approach to the critical issues.     

Chromosomal genes conferring tolerance to heavy metal (Ag) toxicity

Bacterial strains were isolated from sediment samples from the Thames River. Successive transfer growth of the various strains on nutrient agar containing increasing concentrations of AgNO₃ revealed that three of the bacterial isolates were found to be capable of tolerating high concentrations of AgNO₃ ranging from 20 to 80 mM on a solid medium and up to 10 mM AgNO₃ in liquid medium. Molecular characterization and identification based on 16S rDNA gene sequencing of three strains of bacteria that are tolerant to silver nitrate showed that the major tolerant strains include the superbug, Shewanella oneidensis, Pseudomonas sp. and Bacillus sp. Protein extraction and two-dimensional (2D) sodium dodecyl sulfate SDS-polyacrylamide gel electrophoresis (PAGE) of the protein extracts in bacteria exposed to very high concentrations of AgNO₃ revealed a general reduction in the number of expressed proteins, although two protein spots were conspicuously over expressed in the exposed bacteria compared to control. The N-terminal amino acid sequence analysis of the protein spots identified the major up-regulated proteins as the outer membrane protein To1C (45.2 kDa) and the structural protein of the flagellar filament, flagellin (28.34 kDa), encoded for by the to1C and fliC genes, respectively. The roles of these genes in a number of multi-drug resistant pathogen and potentials for biotechnological applications in toxic metal control for treatment of contaminated ecosystems and biomining were discussed.     

summary of the California aerosol characterization experiment

This report gives a brief summary of the experimental plan and the early results of field observations from the California Aerosol Characterization Experiment (ACHEX). The objectives of the program center on questions of the sources and evolution of aerosols in urban air, as they are superimposed on a natural background, particularly as they relate to visibilitydegradation. The project initially nvolved the use of an elaborately instrumented mobile laboratory combined with a fixed station in San Jose, in Pasadena, and in Riverside, and later incorporated several satellite monitoring stations in the Los Angeles Basin. Intensive field observationswere taken from July 1972 to November 1972 in several urban and nonurban locations between the San Francisco Bay area and the South Coast Basin, covering the Los Angeles area. In a second phase of the ACHEX, aerosols accompanying photochemical smog were studied intensively in the Los Angeles Basin during the period between July and October of 1973. The observations cover a wide variety of parameters including physical and chemical properties of aerosols, pollutant gas concentrations, and meteorological variables. The initial results show the great importance of sulfate, nitrate, noncarbonate carbon, and liquid water to the mass concentration of airborne particles and their relation to reduction in visibility. The anthropogenic contribution to atmospheric aerosols is consistently found to be allocated primarily to the submicron or fine particle size range.     

Feasibility of detection and quantification of gas-phase carbonyls in indoor environments using PFBHA derivatization and solid-phase microextraction (SPME)

Solid-phase microextraction (SPME) was evaluated for the detection and quantification of the gas-phase carbonyls: citronellal, glyoxal, methylglyoxal, and beta-ionone. Prepared air samples containing the carbonyl compounds were collected at a flow rate of 2.8 L min(-1) in an impinger containing a 25% reagent water/75% methanol collection liquid. The aqueous samples were then derivatized with O-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine hydrochloride (PFBHA), extracted with a PDMS/DVB coated SPME fiber, and analyzed by GC-MS. Detection limits with a sample air volume of 76 L were calculated to be 0.03 ppbv, 0.34 ppbv, 0.12 ppbv, and 0.28 ppbv for citronellal, glyoxal, methylglyoxal, and beta-ionone, respectively.     

Metabolic activity and elemental composition of krill and other zooplankton from Prydz Bay,Antarctica, during early summer (November–December)

Oxygen uptake and ammonia excretion rates, and body carbon and nitrogen contents were measured in krill (Euphausia superba) and eight other zooplankton species collected during November–December 1982 in the Prydz Bay, Antarctica. From these data, metabolic O:N ratios (by atoms), body C:N ratios (by weight) and daily metabolic losses of body carbon and nitrogen were calculated as a basis from which to evaluate seasonal differences in metabolism and nutritional condition. Comparison of the present data with mid-summer (January) data revealed that early-summer E. superba were characterized by higher metabolic O:N ratios (58.7 to 103, compared with 15.9 to 17.5 for mid-summer individuals). Higher O:N ratios of early-summer E. superba resulted largely from reduced ammonia excretion rates and, to a lesser degree, from slightly increased oxygen uptake rates. Body C:N ratios of E. superba were low in early-summer (3.8 to 4.2) compared with mid-summer krill (4.1 to 4.7) due to lowered body-carbon contents in the former (42.6 to 43.6% compared with 43.2 to 47.5% dry weight of midsummer individuals); gravid females formed an exception, since no seasonal differences in body elemental composition were detected for these. No significant changes in water content (75.3 to 81.4% wet wt) and nitrogen content (9.9 to 11.1% dry wt) in E. superba were evident between the two seasons. Seasonal differences in metabolic rates and elemental composition were less pronounced in a salp (Salpa thompsoni), but a higher metabolic O:N ratio occurred in early-summer individuals. Interspecific comparison of the seven remaining zooplankton species studied with twelve species from mid-summer zooplankton investigated in an earlier study indicated that higher metabolic O:N ratios in early-summer are characteristic not only of herbivore/filter-feeders, but also of some carnivores/omnivores. No relationship between metabolic O:N ratios and body C:N ratios was apparent either intraspecifically or interspecifically, within or between early-summer and mid-summer seasons.     

Measurement of organic compound emission using small test chambers

Organic compounds emitted from a variety of indoor materials have been measured using small (166 L) environmental test chambers. The paper discusses: a) factors to be considered in small chamber testing; b) parameters to be controlled; c) the types of results obtained. The following types of materials have been tested: adhesives, caulks, pressed wood products, floor waxes, paints, and solid insecticides. Selected data are presented. For each material, chamber concentrations of organic compounds have been determined for a range of environmental conditions (e.g., air exchange rate, temperature and relative humidity). Emission rates for individual organic compounds, as well as total measured organics, were calculated. The effects of environmental variables on emission rates have been evaluated. Models are used to evaluate the effect of chamber walls and concentration on emission rates.     

Integrated mite control in apples in Queensland, Australia

An interdisciplinary team introduced Integrated Mite Control (IMC) to the Queensland apple industry in Australia from 1989 to 1992. Project funding supported the employment of a consultant to monitor mite levels and provide management advice to growers who paid for the service. Interviews at the beginning and end of the project showed widespread adoption of IMC over this period. Overall use of chemicals was not reduced, but the use pattern changed towards wider use of chemicals that are less toxic to predatory mites. The future of IMC in the Queensland apple industry is in doubt because the seasonal nature of apple production does not allow the paid consultancy service to be self-sustaining. Indications are that the integrated approach to pest management may not be generally accepted as a strategic management process while the availability of effective chemicals allows the continuation of a spray routine for mite control. The lack of availability of effective and proven methods of pest control was seen by growers as the major constraint to adoption of IMC.     

Agricultural Production Monitoring in the Sahel Using Remote Sensing: Present Possibilities and Research Needs

The main objective is to review the current status of research related to the monitoring of agricultural production in the Sahel (west Africa). The Sahel suffers from frequent shortages of food. It is therefore important to have a tool to monitor environmental variables, and thus crop production, during the agricultural season. Satellite remote sensing can contribute significantly to such a system by collecting information on crops and on environmental variables at a sub-continental geographical scale and with a high temporal frequency. One part of the problem is to estimate crop acreage. The technique of area-sampling frame has to adapted to the Sahelian landscape, which is dominated by traditional farming systems. The second part is to estimate crop yields. Three main approaches exist: statistical, semi-deterministic or deterministic. The use of vegetation indices is discussed as well as techniques to derive biophysical variables from remotely-sensed data. Finally, the integration of these remote-sensing techniques with crop-growth models is discussed and some research needs are identified. It is argued that the quantitative assessment of agricultural production in the Sahel should be based on the integration of remotely-sensed data with semi-deterministic agrometeorological models. This approach will allow a regionalization of the production estimates.