Long-term dispersion and availability of metals from submarine mine tailing disposal in a fjord in Arctic Norway

Mining of Cu took place in Kvalsund in the Arctic part of Norway in the 1970s, and mine tailings were discharged to the inner part of the fjord, Repparfjorden. Metal speciation analysis was used to assess the historical dispersion of metals as well as their potential bioavailability from the area of the mine tailing disposal. It was revealed that the dispersion of Ba, Cr, Ni, Pb and Zn from the mine tailings has been limited. Dispersion of Cu to the outer fjord has, however, occurred; the amounts released and dispersed from the mine tailing disposal area quantified to be 2.5–10 t, less than 5% of Cu in the original mine tailings. An estimated 80–390 t of Cu still remains in the disposal area from the surface to a depth of 16 cm. Metal partitioning showed that 56–95% of the Cu is bound in the potential bioavailable fractions (exchangeable, reducible and oxidisable) of the sediments, totalling approximately 70–340 t, with potential for continuous release to the pore water and re-precipitation in over- and underlying sediments. Surface sediments in the deposit area were affected by elevated Cu concentrations just above the probable effect level according to the Norwegian sediment quality criteria, with 50–80% Cu bound in the exchangeable, reducible and oxidisable fractions, potentially available for release to the water column and/or for uptake in benthic organisms.     

Intermittent agitation of liquid manure: effects on methane,microbial activity,and temperature in a farm-scale study

Liquid manure storages are a significant source of methane (CH₄) emissions. Farmers commonly agitate (stir) liquid manure prior to field application to homogenize nutrients and solids. During agitation, manure undergoes mechanical stress and is exposed to the air, disrupting anaerobic conditions. This on-farm study aimed to better understand the effects of agitation on CH₄ emissions, and explore the potential for intentional agitation (three times) to disrupt the exponential increase of CH₄ emissions in spring and summer. Results showed that agitation substantially increased manure temperature in the study year compared to the previous year, particularly at upper- and mid-depths of the stored manure. The temporal pattern of CH₄ emissions was altered by reduced emissions over the subsequent week, followed by an increase during the second week. Microbial analysis indicated that the activity of archaea and methanogens increased after each agitation event, but there was little change in the populations of methanogens, archaea, and bacteria. Overall, CH₄ emissions were higher than any of the previous three years, likely due to warmer manure temperatures that were higher than the previous years (despite similar air temperatures). Therefore, intermittent manure agitation with the frequency, duration, and intensity used in this study is not recommended as a CH₄ emission mitigation practice.

Implications: The potential to mitigate methane emissions from liquid manure storages by strategically timed agitation was evaluated in a detailed farm-scale study. Agitation was conducted with readily-available farm equipment, and targeted at the early summer to disrupt methanogenic communities when CH₄ emissions increase exponentially. Methane emissions were reduced for about one week after agitation. However, agitation led to increased manure temperature, and was associated with increased activity of methanogens. Overall, agitation was associated with similar or higher methane emissions. Therefore, agitation is not recommended as a mitigation strategy.     

Fate of bisphenol A during treatment with the litter-decomposing fungi Stropharia rugosoannulata and Stropharia coronilla

Bisphenol A is an endocrine disrupting compound, which is ubiquitous in the environment due to its wide use in plastic and resin production. Seven day old cultures of the litter-decomposing fungus Stropharia coronilla removed the estrogenic activity of bisphenol A (BPA) rapidly and enduringly. Treatment of BPA with purified neutral manganese peroxidase (MnP) from this fungus also resulted in 100% reduction of estrogenic activity, as analyzed using a bioluminescent yeast assay, and in the formation of polymeric compounds. In cultures of Stropharia rugosoannulata, estrogenic activity also quickly disappeared but temporarily re-emerged in the further course of cultivation. LC-MS analysis of the extracted estrogenic culture liquid revealed [M−H]⁻ ions with m/z values of 219 and 235. We hypothesize that these compounds are ring fission products of BPA, which still exhibit one intact hydroxyphenyl group to interact with estrogen receptors displayed by the yeast.     

Anti-oviposition activities of used sock media against a dengue vector: prospects of eco-friendly control and solutions to pollution

Yearly, huge amounts of sock refuse are discarded into the environment. Socks contain many molecules, and worn ones, which are rich in smell-causing bacteria, have a strong influence on animals’ behaviors. But the impacts of sock odor on the oviposition behavior of dengue vectors are unknown. We assessed whether Aedes albopictus changes its oviposition activity in response to the presence of used socks extract (USEx) in potential breeding grounds, using choice and no-choice bioassays (NCB). When furnished even chances to oviposit in two sites holding USEx and two others containing water (control), Ae. albopictus deposited significantly less eggs in USEx than in water sites. A similar pattern of oviposition preference was also observed when there were more oviposition options in water. When there were greater oviposition opportunities in USEx sites, Ae. albopictus oviposited preferentially in water. Females laid significantly more eggs during the NCB involving water than USEx. Also, significantly more mature eggs were retained by females in the NCB with USEx than in that with water. These observations strongly suggest the presence of molecules with either repellent or deterrent activities against Ae. albopictus females and provide an impetus to advocate the integration of used socks in dengue control programs. Such applications could be a realistic end-of-life recourse to reroute this waste from landfills.

     

Modeling Nutrient Transports and Exchanges of Nutrients Between Shallow Regions and the Open Baltic Sea in Present and Future Climate

We quantified horizontal transport patterns and the net exchange of nutrients between shallow regions and the open sea in the Baltic proper. A coupled biogeochemical-physical circulation model was used for transient simulations 1961-2100. The model was driven by regional downscaling of the IPCC climate change scenario A1B from two global General Circulation Models in combination with two nutrient load scenarios. Modeled nutrient transports followed mainly the large-scale internal water circulation and showed only small circulation changes in the future projections. The internal nutrient cycling and exchanges between shallow and deeper waters became intensified, and the internal removal of phosphorus became weaker in the warmer future climate. These effects counteracted the impact from nutrient load reductions according to the Baltic Sea Action Plan. The net effect of climate change and nutrient reductions was an increased net import of dissolved inorganic phosphorus to shallow areas in the Baltic proper.     

Climate variability and changes in the major cities of Bangladesh: observations,possible impacts and adaptation

High population density, inadequate infrastructure and low adaptive capacity have made the urban population of Bangladesh highly vulnerable to climate change. Trends in climate and climate-related extreme events in five major cities have been analyzed in this paper to decipher the variability and ongoing changes in urban Bangladesh. An analysis of 55 years (1958–2012) of daily rainfall and temperature data using nonparametric statistical methods shows a significant increase in annual and seasonal mean daily maximum and minimum temperatures in all five cities. A significant increase in climate-related extreme events, such as heavy rainfall events (>20 mm), hot days (>32 °C) and hot nights (>25 °C), is also observed. Climate model results suggest that these trends will continue through the twenty-first century. Vulnerability of urban livelihoods and physical structures to climate change is estimated by considering certainty and timing of impacts. It has been predicted that public health and urban infrastructures, viz. water and power supply, would be the imminent affected sectors in the urban areas of Bangladesh. Adaptation measures that can be adopted to mitigate the negative impacts of climate change are also discussed.     

Mercury contamination in the estuaries and coastal sediments of the Strait of Malacca

Fishers’ local ecological knowledge (LEK) is an additional tool to obtain information about cetaceans, regarding their local particularities, fishing interactions, and behavior. However, this knowledge could vary in depth of detail according to the level of interaction that fishers have with a specific species. This study investigated differences in small-scale fishers’ LEK regarding the estuarine dolphin (Sotalia guianensis) in three Brazilian northeast coastal communities where fishing is practiced in estuarine lagoons and/or coastal waters and where dolphin-watching tourism varies from incipient to important. The fishers (N?=?116) were asked about general characteristics of S. guianensis and their interactions with this dolphin during fishing activities. Compared to lagoon fishers, coastal fishers showed greater knowledge about the species but had more negative interactions with the dolphin during fishing activities. Coastal fishing not only offered the opportunity for fishers to observe a wider variety of the dolphin’s behavior, but also implied direct contact with the dolphins, as they are bycaught in coastal gillnets. Besides complementing information that could be used for the management of cetaceans, this study shows that the type of environment most used by fishers also affects the accuracy of the information they provide. When designing studies to gather information on species and/or populations with the support of fishers, special consideration should be given to local particularities such as gear and habitats used within the fishing community.     

Repeatability,Sensitivity, and Uncertainty Analyses of the BANCS Model Developed to Predict Annual Streambank Erosion Rates

Accelerated streambank erosion caused by channel instability can be the leading cause of sediment impairment of streams. Obtaining accurate streambank erosion rates for sediment budgeting and prioritizing mitigation efforts can be difficult and costly. One approach to quantifying streambank erosion rates is through the development and implementation of an empirically derived “Bank Assessment for Non‐point Source Consequences of Sediment” (BANCS) model. This study aims to improve the BANCS model application by evaluating repeatability between users and identifying sensitive and/or uncertain model inputs. Statistical analysis of streambank evaluations conducted by 10 different individuals suggests the implementation of the BANCS model may not be repeatable. This finding may be due to sensitive model inputs, such as streambank height and near‐bank stress level prediction method selection, and/or uncertain model inputs, such as bank material identification and the associated adjustment of erosion potential. Furthermore, it was found assessing streambanks as a group by obtaining a measure of central tendency from individual evaluations, as well as obtaining a higher level of training, may improve model implementation precision. Application of these suggestions may result in improved prediction of streambank erosion rates utilizing the BANCS model methodology.     

Docosahexaenoic acid accumulation in thraustochytrids: search for the rationale

Thraustochytrid protists characteristically accumulate high amounts of lipids, much of which is comprised of the polyunsaturated fatty acid, docosahexaenoic acid (DHA). Since DHA is important in human health, thraustochytrids have attracted much attention from the point of view of biotechnology. However, the biological rationale of DHA production in the storage lipids of these organisms is not clear. We carried out three experiments to study this. (1) The fate of lipids in thraustochytrids during development was studied by staining lipids of vegetative cells with the fluorescent vital stain for lipids, namely Nile blue, following the growth of the cells. The fluorescent lipid bodies decreased in abundance in freshly formed motile limaciform amoeboid cells and subsequently disappeared when they moved away. When vegetative cells produced vast extensions of plasma membrane, the ectoplasmic net elements (EN), the fluorescent lipids appeared to be transported to them. (2) Changes in lipids and DHA during starvation were examined in cells with enhanced lipid and DHA contents and those in which they were not enhanced. Cells in which lipids and DHA were enhanced by refrigerating them for 48 h survived starvation for a longer period. Compared to cells that had not been refrigerated, total fatty acids, as well as DHA were marginally higher in pre-refrigerated cells, while palmitic acid levels were lower. Starvation of cells resulted in a gradual decrease of absolute concentrations and percentage levels of DHA after 8 days, while percentage of palmitic acid levels increased. (3) The relationship between DHA and specific gravity of cells was studied by comparing cells pre-refrigerated as above with those which were not pre-refrigerated. Refrigerated cells with increased lipids and DHA showed higher specific gravities than non-refrigerated cells. We suggest the following roles for storage lipids and DHA in thraustochytrids: (1) lipids serve as energy sources during movement of cells and for production of EN; (2) DHA in storage lipids become distributed in the plasma membrane when EN are formed; (3) DHA is preferably utilized as a fatty acid energy reserve during starvation and (4) an unknown mechanism results in negative buoyancy of cells when total lipids and DHA are marginally enhanced. DHA in storage lipids might be crucial to the survival of thraustochytrid cells.     

Temporal increase in nest defence intensity of the willow tit (Parus montanus): parental investment or methodological artifact?

Summary Nest defence responses of experimentally naive and revisited willow tits (Parus montanus) towards a predator model were studied near Oulu, northern Finland, in 1988–1989. Intensity was measured with three variables in 228 trials at 90 nests. Two hypotheses explaining the temporal changes in the nest defence behavior were examined. In naive birds the nest defence intensity was positively correlated with the brood age, supporting the parental investment hypothesis. The number of previous trials did not cause additional variation in nest defence behavior in comparison between naive and revisited birds. Similarly, experiences in a previous breeding attempt did not affect the nest defence behavior during the course of the subsequent brood. Thus, the temporal increase in multiply visited nests was not due to the birds becoming familiar with the nest threat, i.e., no support was found for the so-called positive reinforcement hypothesis. We think that methodological problems in avian nest defence studies can hide the adaptive significance of the behavior, not explain it. Offprint requests to: S. Rytkönen