Constitutive or induced defences - how does <Emphasis Type="Italic">Eucalyptus globulus</Emphasis> defend itself from larval feeding?

Summary. Following herbivory, induced responses involving plant secondary metabolites have been reported in a number of tree species. Although a wide range of plant secondary metabolites appear to operate as constitutive plant defences in trees belonging to the Eucalyptus genus, no induced responses have as yet been reported following foliar-chewing insect damage. We empirically tested whether branch defoliation (artificial and larval) of 2-year-old Eucalyptus globulus Labill. trees altered the abundance of specific plant secondary metabolites immediately (3 months after initial larval feeding) and 8 months after the cessation of larval feeding. Metabolites assayed, included essential oils, polyphenolic groups and foliar wax compounds and in all cases their abundance was not significantly altered by defoliation. However, the level of foliar tannins after 3 months of larval feeding did display a trend that suggested elevated levels as the result of defoliation, though this trend was not evident 8 months later, indicating that, if real, the response was a rapid and not a delayed induced response. The level of foliar tannins was also negatively correlated to both average larval survival and average percentage branch defoliation, suggesting that foliar tannins may operate as toxins and/or anti-feedants to M. privata larval feeding.     

Indications of fitness differences among vocal clans of sperm whales

Cultural variation can affect the genetic evolution of a species if there are consistent cultural differences that contribute to fitness variation between groups of individuals. In this study, measures of the reproductive success of groups of sperm whales from different cultural clans are used as proxies for fitness. We measure reproductive success using population length distributions from acoustic and photographic measurements and visual observations of the presence of calves. The results obtained are generally consistent between methods; there are large and significant differences between the clans in the measures of reproductive success. The results from this study strengthen the case for cultural hitchhiking in sperm whales by indicating that differences in culture between clans correlate with differences in fitness.     

Efficacy of seat-mounted thoracic side airbags in the German vehicle fleet

Objective: Thoracic side airbags (tSABs) deploy within close proximity to the occupant. Their primary purpose is to provide a protective cushion between the occupant and the intruding door. To date, various field studies investigating their injury mitigation has been limited and contradicting. The research develops efficacy estimations associated for seat-mounted tSABs in their ability to mitigate injury risk from the German collision environment.

Methods: A matched cohort study using German In-Depth Accident Study (GIDAS) data was implemented and aims to investigate the efficacy of seat-mounted tSAB units in preventing thoracic injury. Inclusion in the study required a nearside occupant involved in a lateral collision where the target vehicle exhibited a design year succeeding 1990. Collisions whereby a tSAB deployed were matched on a 1:n basis to collisions of similar severity where no airbag was available in the target vehicle. The outcome of interest was an incurred bodily or thoracic regional injury. Through conditional logistic regression, an estimated efficacy value for the deployed tSAB was determined.

Results: A total of 255 collisions with the deployed tSAB matched with 414 collisions where no tSAB was present. For the given sample, results indicated that the deployed tSAB was not able to provide an unequivocal benefit to the occupant thoracic region, because individuals exposed to the deployed tSAB were at equal risk of injury (Thorax Maximum Abbreviated Injury Scale (Tho.MAIS)2+ odds ratio [OR] = 1.04, 95% confidence interval [CI], 0.41–2.62; Tho.MAIS3+ OR = 1.15, 95% CI, 0.41–3.18). When attempting to isolate an effect for skeletal injuries, a similar result was obtained. Yet, when the tSAB was coupled with a head curtain airbag, a protective effect became apparent, most noticeable for head/face/neck (HFN) injuries (OR = 0.59, 95% CI, 0.21–1.65).

Conclusion: The reduction in occupant HFN injury risk associated with the coupled tSAB and curtain airbag may be attributable to its ability to provide coverage over previous mechanisms of injury. Yet, the sole presence of the tSAB showed no ability to provide additional benefit for the occupant's thoracic region. Future work should identify mechanisms of injury in tSAB cases and attempt to quantify improvements in the vehicle's ability to resist intrusion.     

Using Public Participation Geographic Information Systems to Identify Places of Watershed Service Provisioning

In this study, we used public participation geographic information systems methods to collect spatial data identifying places that stakeholders in Mobile Bay, Alabama think are important providers of watershed services. These methods allowed us to spatially analyze participatory data from general public respondents and directly compare them with other scientific data in a geographic information systems database. This study identified which places in the region participants believe are important providers of specific watershed services, including fish nurseries, storm protection, flood protection, and water quality protection, which would likely have public support for conservation. Additionally, we assessed the accuracy of participant watershed service identification using land cover data to identify inconsistencies and participant knowledge gaps. This information can be used to target outreach education efforts. We found that the accuracy with which participants correctly identified places with the necessary land cover to provide each service varied considerably. We believe this to be a useful tool for managers to elicit stakeholder input and to identify knowledge gaps regarding the provisioning of watershed services.     

Assessment of bioavailable fraction of POPS in surface water bodies in Johannesburg City,South Africa,using passive samplers: an initial assessment

In this study, the semipermeable membrane device (SPMD) passive samplers were used to determine freely dissolved concentrations of polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) in selected water bodies situated in and around Johannesburg City, South Africa. The devices were deployed for 14 days at each sampling site in spring and summer of 2011. Time weighted average (TWA) concentrations of the water-borne contaminants were calculated from the amounts of analytes accumulated in the passive samplers. In the area of interest, concentrations of analytes in water ranged from 33.5 to 126.8 ng l^?1 for PAHs, from 20.9 to 120.9 pg l^?1 for PCBs and from 0.2 to 36.9 ng l^?1 for OCPs. Chlorinated pesticides were mainly composed of hexachlorocyclohexanes (HCHs) (0.15–36.9 ng l^?1) and dichlorodiphenyltrichloromethane (DDT) with its metabolites (0.03–0.55 ng l^?1). By applying diagnostic ratios of certain PAHs, identification of possible sources of the contaminants in the various sampling sites was performed. These ratios were generally inclined towards pyrogenic sources of pollution by PAHs in all study sites except in the Centurion River (CR), Centurion Lake (CL) and Airport River (AUP) that indicated petrogenic origins. This study highlights further need to map up the temporal and spatial variations of these POPs using passive samplers.     

Enhanced semipermanent dialysis samplers for long-term environmental monitoring in saturated sediments

The ability to sample in situ natural environmental processes has proven to be challenging when working with redox-sensitive contaminates in saturated sediments in wetland systems, especially within the rhizosphere, where sharp redox gradients are common. Many traditional approaches are invasive and disturb natural sediment chemistry. Through laboratory and field studies, the work presented in this study demonstrates a novel semipermanent dialysis sampler that allows for long-term, anaerobic monitoring of shallow sediments. Dialysis samplers were deployed and tested for over 1 year while being exposed to extremes in climate. These newly designed devices produce statistically reproducible data and capture sensitive redox trends. Results from the newly designed samplers were compared to conventional samplers. Initially, both the new and old designs yielded statistically similar data, but these data diverged over a period of months. The new devices are less invasive, so data gathered from these devices are more likely to be a closer representation of true conditions in the subsurface. By giving reliable data from a consistent location in space, these new samplers represent a significant step forward in capturing spatial and temporal variability in wetland redox chemistry during long-term monitoring.     

Dual poroelastic response of a coal seam to CO2 injection

Although the influence of gas sorption-induced coal deformation on porosity and permeability has been widely recognized, prior studies are all under conditions of no change in overburden stress and effective stress-absent where effective stresses scale inversely with applied pore pressures. Here we extend formalism to couple the transport and sorption of a compressible fluid within a dual-porosity medium where the effects of deformation are rigorously accommodated. This relaxes the prior assumption that total stresses remain constant and allows exploration of the full range of mechanical boundary conditions from invariant stress to restrained displacement. Evolution laws for permeability and related porosity are defined at the micro-scale and applied to both matrix and an assumed orthogonal, regular and continuous fracture system. Permeability and porosity respond to changes in effective stress where sorption-induced strains may build total stresses and elevate effective stresses. Gas accumulation occurs in both free- and adsorbed-phases and due to effective grain and skeletal compressibilities. A finite element model is applied to quantify the net change in permeability, the gas flow, and the resultant deformation in a prototypical coal seam under in situ stresses. Results illustrate how the CO₂ injectivity is controlled both by the competition between the effective stress and the gas transport induced volume change within the matrix system and by the dynamic interaction between the matrix system and the fracture system. For typical parameters, initial injection-related increases in permeability due to reduced effective stresses may endure for days to years but are ultimately countered by long-term reductions in permeability which may decline by an order of magnitude. Models suggest the crucial role of stresses and the dynamic interaction between matrix and fractures in correctly conditioning the observed response.     

Measurements of primary trace gases and NOY composition in Houston,Texas

Concentrations of CO, SO₂, NO, NO₂, and NO_Y were measured atop the University of Houston's Moody Tower supersite during the 2006 TexAQS-II Radical and Aerosol Measurement Project (TRAMP). The lowest concentrations of all primary and secondary species were observed in clean marine air in southerly flow. SO₂ concentrations were usually low, but increased dramatically in sporadic midday plumes advected from sources in the Houston Ship Channel (HSC), located NE of the site. Concentrations of CO and NO_x displayed large diurnal variations in keeping with their co-emission by mobile sources in the Houston Metropolitan Area (HMA). CO/NO_x emission ratios of 5.81 ± 0.94 were observed in the morning rush hour. Nighttime concentrations of NO_x (NO_x = NO + NO₂) and NO_Y (NO_Y = NO + NO₂ + NO₃ + HNO₃ + HONO + 21N₂O₅ + HO₂NO₂ + PANs + RONO₂ + p-NO₃^? + …) were highest in winds from the NNW-NE due to emission from mobile sources. Median ratios of NO_x/NO_Y were approximately 0.9 overnight, reflecting the persistence and/or generation of NO_Z (NO_Z = NO_Y ? NO_x) species in the nighttime Houston boundary layer, and approached unity in the morning rush hour. Daytime concentrations of NO_x and NO_Y were highest in winds from the HSC. NO_x/NO_Y ratios reached their minimum values (median ca 0.63) from 1300 to 1500 CST, near local solar noon, and air masses often retained enough NO_x to sustain additional O₃ formation farther downwind. HNO₃ and PANs comprised the dominant NO_Z species in the HMA, and on a median basis represented 17–20% and 12–15% of NO_Y, respectively, at midday. Concentrations of HNO₃, PANs, and NO_Z, and fractional contributions of these species to NO_Y, were at a maximum in NE flow, reflecting the source strength and reactivity of precursor emissions in the HSC. As a result, daytime O₃ concentrations were highest in air masses with HSC influence. Overall, our findings confirm the impact of the HSC as a dominant source region within the HMA. A comparison of total NO_Y measurements with the sum of measured NO_Y species (NO_Yi = NO_x + HNO₃ + PANs + HONO + p-NO₃^?) yielded excellent overall agreement during both day ([NO_Y](ppb) = ([NO_Yi](ppb)11.03 ± 0.16) ? 0.42; r² = 0.9933) and night ([NO_Y](ppb) = ([NO_Yi](ppb)11.01 ± 0.16) + 0.18; r² = 0.9975). A similar comparison between NO_Y–NO_x concentrations and the sum of NO_Zi (NO_Zi = HNO₃ + PANs + HONO + p-NO₃^?) yielded good overall agreement during the day ([NO_Z](ppb) = ([NO_Zi](ppb)11.01 ± 0.30) + 0.044 ppb; r² = 0.8527) and at night ([NO_Z](ppb) = ([NO_Zi](ppb)11.12 ± 0.69) + 0.16 ppb; r² = 0.6899). Median ratios of NO_Z/NO_Zi were near unity during daylight hours but increased to approximately 1.2 overnight, a difference of 0.15–0.50 ppb. Differences between NO_Z and NO_Zi rarely exceeded combined measurement uncertainties, and variations in NO_Z/NO_Zi ratios may have resulted solely from errors in conversion efficiencies of NO_Y species and changes in NO_Y composition. However, nighttime NO_Z/NO_Zi ratios and the magnitude of NO_Z ? NO_Zi differences were generally consistent with recent observations of ClNO₂ in the nocturnal Houston boundary layer.     

Beyond testimonials: Learning from a quality circles programme

Quality circles programmes are based on the assumptions that employee participation leads to valued outcomes such as intrinsic satisfaction and recognition, and that it also results in the implementation of changes which enhance productivity and satisfaction. An in-depth case study of one such programme finds that generally favourable testimonials by participants may be misleading. This programme yielded a few minor changes but little impact on productivity and attitudes in the unit as a whole. Causes for its demise are discussed.     

Perceptions of Species Abundance,Distribution, and Diversity: Lessons from Four Decades of Sampling on a Government-Managed Reserve

/ We examined data relative to species abundance, distribution, anddiversity patterns of reptiles and amphibians to determine how perceptionschange over time and with level of sampling effort. Location data werecompiled on more than one million individual captures or observations of 98species during a 44-year study period on the US Department of Energy's(DOE) Savannah River Site National Environmental Research Park (SRS-NERP) inSouth Carolina. We suggest that perceptions of herpetofaunal speciesdiversity are strongly dependent on level of effort and that land managementdecisions based on short-term data bases for some faunal groups could resultin serious errors in environmental management. We provide evidence thatacquiring information on biodiversity distribution patterns is compatiblewith multiyear spatially extensive research programs and also provide aperspective of what might be achieved if long-term, coordinated researchefforts were instituted nationwide.To conduct biotic surveys on government-managed lands, we recommend revisionsin the methods used by government agencies to acquire and report biodiversitydata. We suggest that government and industry employees engaged inbiodiversity survey efforts develop proficiency in field identification forone or more major taxonomic groups and be encouraged to measure the status ofpopulations quantitatively with consistent and reliable methodologies. Wealso suggest that widespread academic cooperation in the dissemination ofinformation on regional patterns of biodiversity could result byestablishment of a peer-reviewed, scientifically rigorous journal concernedwith status and trends of the biota of the United States. KEY WORDS: Abundance; Amphibian; Biodiversity; Distribution; Landmanagement; Reptile