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71.
Aline S. Turque Alexander M. Cardoso Cynthia B. Silveira Ricardo P. Vieira Flávia A. D. Freitas Rodolpho M. Albano Alessandra M. Gonzalez Rodolfo Paranhos Guilherme Muricy Orlando B. Martins 《Marine Biology》2008,155(2):135-146
In this study we performed a survey of the bacterial communities associated with the Western Atlantic demosponges Hymeniacidon heliophila and Polymastia janeirensis, based on 16S rRNA sequencing and transmission electron microscopy (TEM). We compared diversity and composition of the sponge-associated
bacteria to those of environmental bacteria, represented by free-living bacterioplankton and by bacteria attached to organic
particulate matter in superficial sediments. Partial bacterial 16S rRNA sequences from seawater, sediment, and sponges were
retrieved by PCR, cloning, and sequencing. Sequences were subjected to rarefaction analyses, phylogenetic tree construction,
and LIBSHUFF quantitative statistics to verify coverage and similarity between libraries. Community structure of the free-living
bacterioplankton was phylogenetically different from that of the sponge-associated bacterial assemblages. On the other hand,
some sediment-attached bacteria were also found in the sponge bacterial community, indicating that sponges may incorporate
bacteria together with sediment particles. Rare and few prokaryotic morphotypes were found in TEM analyses of sponge mesohyl
matrix of both species. Molecular data indicate that bacterial richness and diversity decreases from bacterioplankton, to
particulate organic sediment, and to H. heliophila and P. janeirensis. Sponges from Rio de Janeiro harbor a pool of novel and exclusive sponge-associated bacterial taxa. Sponge-associated bacterial
communities are composed of both taxons shared by many sponge groups and by species-specific bacteria. 相似文献
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Tânia Vieira Madureira Susana Velhote Cláudia Santos Catarina Cruzeiro Eduardo Rocha Maria João Rocha 《Environmental science and pollution research international》2014,21(9):6089-6098
A new and fully validated QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) extraction and gas chromatography–tandem mass spectrometry methodology was developed and subsequently implemented for the quantification of 16 polycyclic aromatic hydrocarbons (PAHs) in wild (from Matosinhos Beach, Portugal) and commercial (from Ria de Arousa, Spain) mussels. The method proved to be robust, precise, and accurate, with recoveries ranging from 89.2 to 111.8 %. Total sums of 16 PAHs were 52.91 and 37.58 ng/g of wet weight for wild and commercial specimens, respectively. The three- to four-ring PAHs were the most abundant, and a mixture of petrogenic and pyrolytic sources were suspected to occur in both origin areas. Although the contamination levels were below the European regulated limits, specifically for commercial mussels (this despite wild specimens are also consumed), care should be taken in terms of human health, since we are still not aware of the low-dose versus long-term effects, even more in high-risk population groups. 相似文献
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Dissolved organic materials released by the freshwater microalgae Scenedesmus acuminatus were fractionated into low- and high-molecular weight materials, which were investigated for their capacity to bind copper. The high-molecular weight material was also investigated for its monosaccharide composition and is further discussed in relation to the copper binding property. S. acuminatus was grown in batch cultures under laboratory controlled conditions and harvested at the beginning of stationary growth phase when exuded organic materials were obtained. Copper-complexing property of the total exuded organic materials and exopolysaccharides before and after freeze-drying was evaluated by complexometric titrations and Scatchard Plot Analysis of the titration data. The results revealed the presence of two copper-complexing ligands in the total exuded material, but only one in the exopolysaccharide. Stronger copper-complexing ligands are associated to low molecular weight compounds (LogK'1=7.3, LogCL1=-5.6; LogK'2=6.3, LogCL2=-5.1), whereas weaker ligands to the high molecular weight fraction (LogK'2=6.4, LogCL2=-5.6). Although freeze-drying the polymeric organic material (exopolysaccharide) may result in conformational changes of the molecule, no effect on copper-complexing properties was detected. Gas chromatography was used to evaluate the monosaccharide composition of the microalgal exopolysaccharide, which detected high content of mannose and 12% of acid monosaccharides. 相似文献
79.
High molecular weight materials (HMWM, >12000-14000 Da) excreted by the two cyanophyte species (Microcystis aeruginosa and Anabaena spiroides) and a diatom (Aulacoseira granulata) which are dominant phytoplankton species in a eutrophic reservoir, Barra Bonita, Brazil were investigated as copper (Cu) and cadmium (Cd) complexation agents and their monosaccharide and elemental analysis of C, H, N and S determined. Also, HMWM obtained from the reservoir water as well as from a mixture of the three algae materials were studied. The HMWM of the cyanophytes and the mixture of the three algae materials complexed Cu and Cd, whereas the HMWM of the diatom and that from the reservoir water complexed only Cu. Two classes of ligands of intermediate to weak binding strength were obtained after Scatchard plot analysis of the titration data. The cyanophytes and the mixture HMWM presented higher conditional stability constants for Cu class-1 ligands (logK1' = 9.2-9.5) than the HMWM derived from the diatom and the reservoir water (logK1' = 8.6-8.8). Higher proportions of acidic monosaccharides corresponded to higher K1' of Cu and Cd complexation, yet no relation was observed among complexation parameters and elemental analysis. This study points out Cu ligands of intermediate to weak binding strength in the excreted HMWM of dominant microalgae and in the HMWM of the reservoir water, while Cd was solely complexed by ligands isolated from the cyanophyte HMWM. 相似文献
80.
Andressa Cristhy Buch Maria Elizabeth Fernandes Correi Daniel Cabral Teixeir Emmanoel Vieira Silva-Filho 《环境科学学报(英文版)》2015,27(6):217-227
The increasing levels of mercury (Hg) found in the atmosphere arising from anthropogenic sources, have been the object of great concern in the past two decades in industrialized countries. Brazil is the seventh country with the highest rate of mercury in the atmosphere. The major input of Hg to ecosystems is through atmospheric deposition (wet and dry), being transported in the atmosphere over large distances. The forest biomes are of strong importance in the atmosphere/soil cycling of elemental Hg through foliar uptake and subsequent transference to the soil through litter, playing an important role as sink of this element. Soil microarthropods are keys to understanding the soil ecosystem, and for such purpose were characterized by the soil fauna of two Units of Forest Conservation of the state of the Rio de Janeiro, inwhich one of the areas suffer quite interference from petrochemicals and industrial anthropogenic activities and other area almost exempts of these perturbations. The results showed that soil and litter of the Atlantic Forest in Brazil tend to stock high mercury concentrations, which could affect the abundance and richness of soil fauna, endangering its biodiversity and thereby the functioning of ecosystems. 相似文献