Subcellular distribution and toxicity of cadmium in Potamogeton crispus L

The submerged macrophyte Potamogeton crispus L. was subjected to varying doses of cadmium (0, 20, 40, 60 and 80 μM) for 7 d, and the plants were analyzed for subcellular distribution of Cd, accumulation of mineral nutrients, photosynthesis, oxidative stress, protein content, and ultrastructural distribution of calcium (Ca). Leaf fractionation by differential centrifugation indicated that 48-69% of Cd was accumulated in the cell wall. At all doses of Cd, the levels of Ca and B rose and the level of Mn fell; the levels of Fe, Mg, Zn, Cu, Mo, and P rose initially only to decline later. Exposure to Cd caused oxidative stress as evident by increased content of malondialdehyde and decreased contents of chlorophyll and protein. Photosynthetic efficiency, as indicated by the quenching of chlorophyll a fluorescence (Fv/Fm, Fo and Fm), decreased significantly, the extent of decrease being directly proportional to the concentration of Cd. Increased amounts of precipitates of calcium were noticed in the treated plants, located either outside the cell membrane or in chloroplasts, mitochondria, the nucleus, and the cytoplasm whereas control plants showed small deposits of the precipitates around surface of the vacuole membrane and in the intercellular space but rarely in the cytoplasm. Photosynthetic efficiency and oxidative stress could be used as indicators of physiological end-points in determining the extent of Cd phytotoxicity.     

离子色谱法测定大气中的甲磺酸

建立了以玻璃纤维膜-KB120气溶胶采样器采集大气中甲磺酸的采样方法;以0.1mmol·l-1H3BO3/0.1mmol·l-1 Na2B4O7为淋洗液的甲磺酸离子色谱分析方法.分析方法的精密度和准确度分别为5%和4%.并实测了青岛冬季大气气溶胶中MSA,SO2-4和NO-3的平均浓度分别为0.10μg·m-3,30.40μg·m -3和17.98μg·m-3.     

湖北水资源现状与可持续利用战略对策

湖北省有十分丰富的过境客水资源，但自产水资源却十分有限，在水资源开发利用中存在许多亟待解决的问题。如地表水污染日趋严重，地下水也存在不容的污染；农业用水占大头，其节水潜力巨大；目前主要是利用地表水，地下水的利用很少；洪涝时时灾害仍然十分严重，并有愈演愈烈的趋势；从长远看，湖北省的水资源问题将主要是污染型短缺的问题；南水北调中线工程将对汉江中下游供水和水质造成不容忽视的影响；在计划体制下形成的水资源     

The fragmentation patterns of maternal plasma cell-free DNA and its applications in non-invasive prenatal testing

The discovery of cell-free DNA (cfDNA) in maternal plasma has opened up new promises for the development of non-invasive prenatal testing (NIPT). Application of cfDNA in NIPT of fetus diseases and abnormalities is restricted by the low amount of fetal DNA molecules in maternal plasma. Fetus-derived cfDNA in maternal plasma are shorter than maternal DNA, thus leveraging the maternal and fetus-derived cfDNA molecules size difference has become a novel and more accurate method for NIPT. However, multiple biological properties such as size distribution of plasma DNA, proportion of fetal-derived DNA and methylation levels in maternal plasma across different gestational ages still remain largely unknown. Further insights into the size distribution and fragmentation pattern of circulating plasma cfDNA will shed light on the origin and fragmentation mechanisms of cfDNA during physiological and pathological processes in prenatal diseases and enhance our ability to take the advantage of plasma cfDNA as a molecular diagnostic tool. In the review, we start by summarizing the research techniques for the determination of the fragmentation profiles of cfDNA in maternal plasma. We then summarize the main progress and findings in size profiles of maternal plasma cfDNA and cffDNA. Finally, we discuss the potential diagnostic applications of plasma cfDNA size profiling.     

生物滤池对气相与液相中污染物质的净化

通过实验室研究探讨了生物滤池同时处理废水和废气的可行性。试验结果表明：在２５℃－３７℃条件下，甲苯废气的流量为９０Ｌ／ｈ，甲苯浓度为５００ｍｇ／ｍ＾３；废水的流量为０．４Ｌ／ｈ，ＣＯＤｃｒ浓度为４００－７５０ｍｇ／Ｌ，甲苯与ＣＯＤｃｒ的去除率分别是７０％－７２％和８６％－８９％。     

循环经济促进法律责任研究

循环经济促进法律责任是主体共同而有区别的责任,主要体现为清洁生产、能源节约、生态设计、可持续的消费和环境友好的废物管理等方面的义务要求.环境外部性理论和环境法基本原则为循环经济促进法律责任设计提供了理论依据.国家的循环经济促进法律责任的主要内容是制定并监督实施符合循环经济要求的基本法律、政策、标准和要求等.企业的循环经济促进法律责任主要是采用无毒、易降解、资源和能源消耗少、耐用、便于回收和再生利用的环境友好产品设计和生产技术等.消费者的循环经济法律赍任是:通过支付价格为产品的环境影响承担经济责任.同时对责任配置的规则、延伸生产者责任的合理性与限度进行了探讨,主张我国循环经济促进法律责任设计应重视成本收益分析、考虑具体国情,实行强制与经济激励并重.     

2008年初湖北省雨雪过程酸雨特征及成因

利用湖北省32个气象酸雨监测站在2008年初雨雪过程中的306次有效降水样品检测资料及相关气象环境资料,分析了此次雨雪过程的酸雨特征及成因,以期对降雪酸沉降的深入研究有所帮助.分析结果表明,全省平均pH值为3.97,酸雨发生频率为89.2%,强酸雨发生频率达到64.4%,均高于历史同期平均水平.32个气象酸雨监测站的降水平均pH值除孝感外均呈酸雨,其中20站酸雨发生频率达到100%.此次雨雪过程酸雨强度大且频发是由多方面原因综合影响所致,首先,强盛的西南气流有利于长江以南重酸雨区污染物的迁移,其次,低温导致燃煤用量增加,引起酸性物质排放的增多,而较强的逆温层又使污染物扩散条件变差,此外,雪花较雨滴更慢的降落速度和更大的比表面积使其能够更充分的捕集和吸收大气中的酸性物质.     

高墩大跨连续刚构桥施工期安全评价

针对高墩大跨连续刚构桥施工期结构概率失效的特性,提出了基于失效概率的安全评价方法.以徐水沟特大桥为工程背景,通过风险评定识别出施工最不利阶段--最大双悬臂时的主要风险因素即:节段特性模量的不确定性、梁体自重的不确定性以及预应力损失的不确定性.采用大型通用有限元程序ANSYS及蒙特卡洛法,计算徐水沟特大桥在最大悬臂时的失效概率,并确定施工期的目标可靠度.依据高墩大跨连续刚构桥施工期结构概率失效的评价结果,提出相应的风险控制措施.     

克拉玛依市机动车污染现状调查与防治对策研究

在调查克拉玛依市机动车尾气污染的基础上,分析存在的问题,并针对性地提出城市交通污染的控制对策.