Methodologies for determination of antimony in terrestrial environmental samples

Methodologies for the environmental analysis of total antimony and aqueous chemical speciation are critically reviewed, including preparation techniques for aqueous and solid matrices and the determination of solid state partitioning and recommendations are given for future research directions. Concentrations of total antimony commonly present in aqueous and solid environmental samples are readily determined using present day analytical techniques. This has resulted primarily from technological advances in microwave digestion for solid matrices and the development of plasma based analyte detection systems. ICP-AES and ICP-MS techniques are both utilised for the environmental analysis of total antimony concentrations. However, ICP-MS is increasingly favoured as a result of reduced spectral interferences and the potential for analyte detection in the pg mL(-1) range. Determination of aqueous antimony speciation presents a number of complex analytical challenges and highly selective separation and identification techniques are required prior to detection. The majority of published techniques including common applications of hydride generation are insufficiently selective for the determination of intrinsic chemical speciation and often only oxidation state data are obtained. The recent in-line applications of HPLC-ICP-MS offer the potential for highly selective separations of aqueous antimony species and determination of detailed chemical speciation data. However, considerable development work is required to optimise chromatographic separations and identify uncharacterised species resident in environmental systems. Analytical techniques to aid the determination of antimony's associations with solid environmental matrices include the application of chemical extraction procedures and leaching experiments. To date, this area of analytical research has received little attention and further studies are required to elucidate this aspect of antimony's environmental chemistry.     

Estimating Evapotranspiration and Seepage for a Sinkhole Wetland From Diurnal Surface‐Water Cycles1

Abstract: This study used measured diurnal surface‐water cycles to estimate daily evapotranspiration (ET) and seepage for a seasonally flooded sinkhole wetland. Diurnal surface‐water cycles were classified into five categories based on the relationship between the surface‐water body and the surrounding ground‐water system (i.e., recharge/discharge). Only one class of diurnal cycles was found to be suitable for application of this method. This subset of diurnal cycles was used to estimate ET and seepage and the relative importance of each transfer process to the overall water budget. The method has limited utility for wetlands with erratic hydrologic regimes (e.g., wetlands in urban environments). This is due to violation of the critical assumption that the inflow/outflow rate remains constant throughout the day. For application to surface‐water systems, the method is typically applied with an assumed specific yield of 1.0. This assumption was found to be invalid for application to surface‐water systems with a noncylindrical pond geometry. An overestimation of ET by as much as 60% was found to occur under conditions of low pond stage and high water loss. The results demonstrate the high ET rates that can occur in isolated wetlands due to contrasting roughness and moisture conditions (oasis and clothesline effects). Estimated ET rates ranged from 4.1 to 18.7 mm/day during the growing season. Despite these large ET rates, seepage (recharge) was found to be the dominant water loss mechanism for the wetland.     

Effects of temperature on feeding and activity in the crab Scylla serrata

The effect of temperature on feeding, duration of emergence and movement by the crab Scylla serrata (Forskal) was measured under laboratory conditions using infrared time-lapse photography. Little difference was found between experiments carried out at 25° and 20°C. All parameters measured declined at 16°C. At 12°C emergence time and movement were 24 and 33% respectively of the level at 25°C. At 25°C, 65% of crabs fed, but none did so at 12°C. No statistically significant difference was found between male and female crabs in the parameters measured.     

Geographic variability of Escherichia coli ribotypes from animals in Idaho and Georgia

Several genotypic methods have been developed for determining the host origin of fecal bacteria in contaminated waters. Some of these methods rely on a host origin database to identify environmental isolates. It is not well understood to what degree these host origin isolates are geographically variable (i.e., cosmopolitan or endemic). This is important because a geographically limited host origin database may or may not be universally applicable. The objective of our study was to use one genotypic method, ribotyping, to determine the geographic variability of the fecal bacterium, Escherichia coli, from one location in Idaho and three locations in Georgia for cattle (Bos taurus), horse (Equus caballus), swine (Sus scrofa), and chicken (Gallus gallus domesticus). A total of 568 fecal E. coli isolates from Kimberly, ID (125 isolates), Athens, GA (210 isolates), Brunswick, GA (102 isolates), and Tifton, GA (131 isolates), yielded 213 ribotypes. The percentage of ribotype sharing within an animal species increased with decreased distance between geographic locations for cattle and horses, but not for swine and chicken. When the E. coli ribotypes among the four host species were compared at one location, the percent of unshared ribotypes was 86, 89, 81, and 79% for Kimberly, Athens, Brunswick, and Tifton, respectively. These data suggest that there is good ribotype separation among host animal species at each location. The ability to match environmental isolates to a host origin database may depend on a large number of environmental and host origin isolates that ideally are not geographically separated.